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    • 6. 发明授权
    • Nanospearing for molecular transportation into cells
    • 纳米粒子用于分子运输到细胞中
    • US07935517B2
    • 2011-05-03
    • US11233481
    • 2005-09-22
    • Dong CaiDavid L. Carnahan
    • Dong CaiDavid L. Carnahan
    • C12M1/42
    • C12N15/89C12M35/02C12N15/87Y10S977/746Y10S977/748
    • A nanostructured molecular delivery vehicle comprising magnetic materials and configured to receive passenger biomolecules. The application of a an appropriate magnetic field having a gradient orients and drives the vehicle into a biological target, which may comprise cells, cell masses, tissue slices, tissues, etc. Under the control of the magnetic field, these vehicles can penetrate cell membranes. Then, the biomolecules carried by the vehicle can be released into the cells to perform their functions. Using this “nanospearing” technique, unprecendented high transfection efficiency has been achieved in several difficult-to-transfect cells. These include, but are not limited to, Bal 17 cells, ex vivo B cells, primary cultured cortical neurons, etc. This method advances the state of the art, providing an improved technique for the introduction of exogenous molecules to cells, with the clinical applications including, but not being limited to, drug delivery, gene therapy, vaccination, etc.
    • 纳米结构分子递送载体,其包含磁性材料并且构造成接收乘客生物分子。 施加具有梯度的合适的磁场使得车辆进入生物靶,其可以包括细胞,细胞块,组织切片,组织等。在磁场的控制下,这些载体可以穿透细胞膜 。 然后,车辆携带的生物分子可以释放到细胞中以发挥其功能。 使用这种“纳米级”技术,在几个难以转染的细胞中已经实现了非常重要的高转染效率。 这些方法包括但不限于Bal 17细胞,离体B细胞,原代培养的皮质神经元等。该方法改进了现有技术,提供了一种用于将外源性分子引入细胞的改进技术,其中临床 应用包括但不限于药物递送,基因治疗,疫苗接种等
    • 9. 发明授权
    • Nanoscale grasping device, method for fabricating the same, and method for operating the same
    • 纳米抓握装置及其制造方法及其操作方法
    • US06950296B2
    • 2005-09-27
    • US10057262
    • 2002-01-25
    • David L. Carnahan
    • David L. Carnahan
    • G01Q60/00G01Q90/00H02N13/00H05F3/00
    • H02N13/00B81C99/002B82Y10/00Y10S977/725
    • A nanoscale grasping device comprising at least three electrostatically actuated grasping elements. The use of at least three elements, which together define a plane, allows an object to be grasped more accurately, more easily held, and more readily manipulated. The grasping elements preferably comprise conductive nanotubes which are grown at specific points on a substrate (e.g., directly on an electrode), using chemical vapor deposition (“CVD”) techniques, thereby allowing the grasping device to be manufactured with greater control. Different types of electrostatic forces may be used to open or close the grasping tool. Such attractive and repulsive forces can be created through the application of either a constant voltage or an oscillating voltage.
    • 包括至少三个静电驱动的抓握元件的纳米级抓握装置。 使用一起限定平面的至少三个元件,允许更准确地,更容易地握住物体并且更容易地操纵物体。 抓握元件优选地包括使用化学气相沉积(“CVD”)技术在衬底(例如,直接在电极上)上的特定点生长的导电纳米管,从而允许以更大的控制制造把持装置。 可以使用不同类型的静电力来打开或关闭把持工具。 这种有吸引力和排斥力可以通过施加恒定电压或振荡电压来产生。