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1. 发明授权

US4628035A Immunoassay method 失效
标题翻译：免疫测定法
公开(公告)号：US4628035A
公开(公告)日：1986-12-09
申请号：US638423
申请日：1984-08-07
申请人： Daizo Tokinaga , Teruaki Kobayashi , Kazumichi Imai
发明人： Daizo Tokinaga , Teruaki Kobayashi , Kazumichi Imai
IPC分类号： A61K39/44 , G01N33/561 , G01N33/58 , G01N33/543
CPC分类号： G01N33/561
摘要： An immunoassay method for measuring a concentration of an antigen for a short period of time by immobilizing an antibody over the whole zone of an effective supporting matrix for electrophoresis and fixing an antigen in a sample to be measured by electrophoresis for the antigen-antibody reaction between said immobilized antibody and said antigen.
摘要翻译：一种免疫测定方法，用于通过将抗体固定在有效支持基质的整个区域上进行电泳和固定待测样品中的抗原，将抗体固定在短时间内的抗原浓度，以通过电泳进行抗原抗体反应所述固定化抗体和所述抗原。

2. 发明授权

US5057438A Electrophoretic antigen-antibody determination with laminate of multiple membranes 失效
标题翻译：电泳抗原 - 抗体用多层膜叠层测定
公开(公告)号：US5057438A
公开(公告)日：1991-10-15
申请号：US31665
申请日：1987-03-30
申请人： Kazumichi Imai , Daizo Tokinaga , Teruaki Kobayashi , Kenji Yasuda , Keiichi Nagai , Satoshi Takahashi
发明人： Kazumichi Imai , Daizo Tokinaga , Teruaki Kobayashi , Kenji Yasuda , Keiichi Nagai , Satoshi Takahashi
IPC分类号： G01N33/561
CPC分类号： G01N33/561 , Y10S436/809 , Y10S436/824
摘要： Determination of a plurality of species of antibodies or antigens is attained by a method which comprises forming a plurality of different kinds of reaction membranes each having a different species of antibody or antigen on an electrophoretic carrier, superposing these reaction membranes, optionally superposing a filter on the laminate of reaction membranes, inserting the laminate in an electrolyte, adding a plurality of different species of antigens or antibodies corresponding to the plurality of species of antibodies or antigens supported in the aforementioned reaction membranes, electrophoretically moving the added antigens or antibodies through the electrolyte and enabling them to react with the antibodies or antigens supported on the reaction membranes, and measuring the concentrations of either the antigens or antibodies resulting from the reaction or the antibodies or antigens supported in an unreacted form on the reaction membranes.
摘要翻译：通过包括在电泳载体上形成多个不同种类的抗体或抗原的不同种类的反应膜，叠加这些反应膜，任选地将过滤器叠加在一起的方法来实现多种抗体或抗原的测定反应膜的层压体，将层压体插入电解质中，加入与上述反应膜中支持的多种抗体或抗原相对应的多种不同种类的抗原或抗体，通过电解质电泳移动加入的抗原或抗体并使其能够与支持在反应膜上的抗体或抗原反应，并测量由反应产生的抗原或抗体的浓度，或反应膜上以未反应形式负载的抗体或抗原的浓度。

3. 发明授权

US4824778A Immunoassay for measuring the concentration of antigen in a sample 失效
标题翻译：用于测量样品中抗原浓度的免疫测定
公开(公告)号：US4824778A
公开(公告)日：1989-04-25
申请号：US867554
申请日：1986-05-28
申请人： Keiichi Nagai , Daizo Tokinaga , Kazumichi Imai , Kenji Yasuda , Satoshi Takahashi , Teruaki Kobayashi
发明人： Keiichi Nagai , Daizo Tokinaga , Kazumichi Imai , Kenji Yasuda , Satoshi Takahashi , Teruaki Kobayashi
IPC分类号： G01N33/543 , G01N33/561 , G01N33/53
CPC分类号： G01N33/561 , Y10S435/968
摘要： An immunoassay comprisingimmobilizing antibody in a matrix for electrophoresis;immobilizing antigen in a measurement sample by subjecting the same to antigen antibody reaction with the above-mentioned immobilized antibody by a procedure of moving the antigen by electrophoresis;either moving labeled antibody to the above-mentioned immobilized antigen by electrophoresis to react the same with the immobilized antigen, or moving labeled antigen to the unreacted portion of the above-mentioned immobilized antibody by electrophoresis to react the same with the unreacted portion; andmeasuring the concentration of antigen in the sample, characterized byusing as a label for the labeled antibody or the labeled antigen an enzyme capable of coverting a substrate into a fluorescent substance,moving the substrate convertible into a fluorescent substance by said enzyme by electrophoresis,reacting the substrate with the label enzyme to convert the same into a fluorescent substance, andmeasuring the concentration of the fluorescent substance in the electrolyte solution.
摘要翻译：免疫测定法，其包括将抗体固定在用于电泳的基质中; 通过使抗原通过电泳移动的方法，将抗原固定在测量样品中，使其与上述固定化抗体进行抗原抗体反应; 通过电泳将标记抗体移动到上述固定化抗原上，使其与固定化抗原反应，或通过电泳将标记的抗原移动到上述固定化抗体的未反应部分，使其与未反应部分反应; 测定样品中的抗原浓度，其特征在于，将标记抗体或标记抗原的标记作为能够将底物覆盖成荧光物质的酶，通过电泳将所述酶转化为荧光物质，使底物与标记酶反应，将其转化为荧光物质，并测量电解质溶液中荧光物质的浓度。

4. 发明授权

US4913883A Particle agglutination immunoassay apparatus 失效
标题翻译：粒子凝集免疫测定仪
公开(公告)号：US4913883A
公开(公告)日：1990-04-03
申请号：US221971
申请日：1988-07-20
申请人： Kazumichi Imai , Daizo Tokinaga , Koichi Yokosawa
发明人： Kazumichi Imai , Daizo Tokinaga , Koichi Yokosawa
IPC分类号： G01N33/543 , G01N35/00
CPC分类号： G01N33/54333 , G01N33/54366 , G01N35/0098
摘要： The degree of agglutination is measured in a magnetic manner instead of optical manner. Microparticles are composed of a magnetic material and the agglutinated condition is measured using a magnetometer. The sizes and distribution of agglutinated materials formed by the antigen-antibody coupling reaction can be correctly measured without affected by light-scattering materials such as proteins or blood cells contained in the specimen, or by absorbant such as pigment, or by fluorescent material. Therefore, the concentration of antigen is measured maintaining high precision.
摘要翻译：以磁性方式代替光学方式测量凝集度。微粒由磁性材料组成，并且使用磁力计测量凝集条件。通过抗原 - 抗体偶联反应形成的凝集物质的大小和分布可以在不受光散射物质如样品中所含的蛋白质或血液细胞，或吸收剂如颜料或荧光材料的影响的情况下被正确地测量。因此，保持高精度地测量抗原的浓度。

5. 发明申请

US20130157264A1 NUCLEIC ACID ANALYSIS DEVICE, NUCLEIC ACID ANALYSIS APPARATUS, AND NUCLEIC ACID ANALYSIS METHOD 审中-公开
标题翻译：核酸分析装置，核酸分析装置和核酸分析方法
公开(公告)号：US20130157264A1
公开(公告)日：2013-06-20
申请号：US13818704
申请日：2011-07-19
申请人： Takayuki Obara , Kazumichi Imai , Toshiro Saito , Satoshi Takahashi
发明人： Takayuki Obara , Kazumichi Imai , Toshiro Saito , Satoshi Takahashi
IPC分类号： G01N21/64
CPC分类号： G01N21/6486 , C12Q1/6837 , G01N21/6428 , G01N21/6452 , G01N21/648 , C12Q2563/173
摘要： In a nucleic acid analysis device which detects a fluorescent dye on a nucleic acid sample immobilized on a surface of a substrate by exciting the fluorescent dye with an evanescent wave, the detection of a fluorescence signal with a high SN ratio is realized even for a long nucleic acid sample.The nucleic acid analysis device according to the invention is a nucleic acid analysis device in which a plurality of regions for immobilizing a nucleic acid sample are provided on a surface of a support base and a single molecule of a nucleic acid sample is immobilized on at least one of the regions, and which performs sequence determination by performing an extension reaction of the immobilized nucleic acid sample, wherein the immobilization of the single molecule of the nucleic acid sample on the support base is performed at two or more points.
摘要翻译：在通过用ev逝波激发荧光染料来检测固定在基板表面上的核酸样品上的荧光染料的核酸分析装置中，即使长时间地实现高SN比的荧光信号的检测核酸样品。根据本发明的核酸分析装置是核酸分析装置，其中在支持基底的表面上提供用于固定核酸样品的多个区域，并且将至少一个核酸样品的单个分子固定在至少其中一个区域，并且通过进行固定化的核酸样品的延伸反应进行序列测定，其中将核酸样品的单分子固定在载体基底上进行两点或更多点。

6. 发明授权

US07662269B2 Capillary electrophoretic instrument and capillary array assembly 有权
标题翻译：毛细管电泳仪和毛细管阵列组件
公开(公告)号：US07662269B2
公开(公告)日：2010-02-16
申请号：US12359115
申请日：2009-01-23
申请人： Muneo Maeshima , Kazumichi Imai , Masaya Kojima , Satoshi Takahashi , Hiromi Yamashita
发明人： Muneo Maeshima , Kazumichi Imai , Masaya Kojima , Satoshi Takahashi , Hiromi Yamashita
IPC分类号： G01N27/453
CPC分类号： G01N27/44713 , G01N27/447 , G01N27/44704 , G01N27/44743 , G01N27/44782
摘要： The troublesomeness during the setting of a plurality of capillaries is eliminated by composing pairs of electrodes, which are electrically connected to the common electrode, and capillaries. By bringing electrodes installed in the vicinity of each capillary disposed at the pitch of wells on the side of sample plate (within the area of the wells) into electrical contact with a common electrode, the capillaries and electrodes are made integral in construction. When a voltage is applied to the electrophoretic instrument via a common electrode portion, the voltage is applied to the electrodes for each capillary. This enables an inexpensive microtiter plate, etc. to be used and a multiple of capillaries to be simultaneously inserted, attached and detached.
摘要翻译：通过构成电连接到公共电极的电极对和毛细管来消除在多个毛细管的设置期间的麻烦。通过将安装在每个毛细管附近的电极安装在样品板侧面（在该区域内）的孔的间距与公共电极电接触，毛细管和电极在结构上是一体的。当通过公共电极部分向电泳仪施加电压时，对每个毛细管的电极施加电压。这使得可以使用廉价的微量滴定板等，同时插入，附着和分离多个毛细管。

7. 发明申请

US20070278101A1 Capillary electrophoresis apparatus 有权
标题翻译：毛细管电泳仪
公开(公告)号：US20070278101A1
公开(公告)日：2007-12-06
申请号：US11806889
申请日：2007-06-05
申请人： Takashi Gomi , Ryoji Inaba , Motohiro Yamazaki , Hidenori Namba , Jin Matsumura , Hiromi Yamashita , Seiichi Ugai , Kazumichi Imai
发明人： Takashi Gomi , Ryoji Inaba , Motohiro Yamazaki , Hidenori Namba , Jin Matsumura , Hiromi Yamashita , Seiichi Ugai , Kazumichi Imai
IPC分类号： G01N27/00
CPC分类号： G01N27/44721
摘要： The present invention provides a capillary electrophoresis apparatus in which a capillary is easily attached to and detached from a migration medium filling unit without mixing impurities into the capillary. Mixture of impurities such as dust is also prevented when a capillary negative-electrode end is brought into contact with a sample stored in a vessel. Furthermore, temperature control is efficiently performed in the capillary. In the capillary electrophoresis apparatus, the whole of capillary array can be supported by grasping a grip portion by hand. A migration medium filling mechanism includes a slide mechanism which moves a polymer block with respect to a capillary head. The capillary electrophoresis apparatus includes a vessel in which a sample and a buffer can simultaneously be stored. Temperatures of the capillary and an irradiation and detection unit are controlled by a temperature control function provided in a thermostatic device.
摘要翻译：本发明提供了一种毛细管电泳装置，其中毛细管容易地附着到迁移介质填充单元上并从迁移介质填充单元中分离而不将杂质混入毛细管中。当毛细管负极端与存储在容器中的样品接触时，也可以防止诸如灰尘等杂质的混合物。此外，在毛细管中有效地进行温度控制。在毛细管电泳装置中，通过用手抓握把手部分可以支撑整个毛细管阵列。迁移介质填充机构包括相对于毛细管头移动聚合物嵌段的滑动机构。毛细管电泳装置包括可以同时存储样品和缓冲液的容器。毛细管和照射和检测单元的温度由恒温装置中提供的温度控制功能控制。

8. 发明授权

US06191425B1 Multicolor fluorescence detection type electrophoretic analyzer 失效
标题翻译：多色荧光检测型电泳分析仪
公开(公告)号：US06191425B1
公开(公告)日：2001-02-20
申请号：US09023561
申请日：1998-02-13
申请人： Kazumichi Imai
发明人： Kazumichi Imai
IPC分类号： G01N2726
CPC分类号： G01N27/44721
摘要： A plurality of samples are separated into sample components by means of electrophoresis in a plurality of capillaries. Light is radiated onto the separated sample components. Fluorescence emitted from the sample components is measured by a detector unit. In the detector unit, the fluorescence from the sample components is collected by a lens, filtered by a filter set to select light of predetermined wavelengths, split into four bundle of rays by right-angle prisms, and focussed on four linear array sensors so as to be detected.
摘要翻译：多个样品通过多个毛细管中的电泳分离成样品成分。光照射到分离的样品组分上。通过检测器单元测量从样品组分发射的荧光。在检测器单元中，来自样品组分的荧光由透镜收集，由滤光片组滤波，以选择预定波长的光，通过直角棱镜分成四束光束，并聚焦在四个线性阵列传感器上，以便被检测。

9. 发明授权

US09207235B2 Nucleic acid analyzer, reaction device for nucleic acid analysis and substrate of reaction device for nucleic acid analysis 有权
标题翻译：核酸分析仪，核酸分析反应装置和核酸分析反应装置底物
公开(公告)号：US09207235B2
公开(公告)日：2015-12-08
申请号：US13575564
申请日：2010-12-01
申请人： Yoshiaki Sugimura , Masatoshi Narahara , Kazumichi Imai , Toshiro Saito , Ryoji Inaba , Takuya Matsui
发明人： Yoshiaki Sugimura , Masatoshi Narahara , Kazumichi Imai , Toshiro Saito , Ryoji Inaba , Takuya Matsui
IPC分类号： B01L3/00 , G01N33/543 , G01N21/64 , G01N33/53 , G01N33/58 , G01N35/00
CPC分类号： G01N33/54313 , B01L3/502715 , B01L2200/0668 , B01L2300/0609 , B01L2300/0877 , B01L2300/0883 , G01N21/6452 , G01N21/648 , G01N33/5308 , G01N33/54373 , G01N33/582 , G01N35/00 , G01N2035/00564
摘要： Provided is a reaction device for nucleic acid analysis wherein microparticles, which carry a nucleic acid to be detected having been immobilized thereon, are aligned in a lattice form on a substrate according to the pixel size of a two-dimensional sensor. By this reaction device for nucleic acid analysis which is provided with a channel-forming reaction chamber on the substrate (101), the nucleic acid having been immobilized on the microparticles (103) on the substrate (101) is detected. The microparticles (103), which carry the nucleic acid to be detected having been immobilized thereon, are arranged by microstructures (102) aligned on the substrate (101).
摘要翻译：提供了用于核酸分析的反应装置，其中携带已被固定在其上的被检测核酸的微粒根据二维传感器的像素尺寸以格子形式排列在基板上。通过在基板（101）上设置有通道形成反应室的核酸分析用反应装置，检测固定在基板（101）上的微粒（103）上的核酸。携带已被固定在其上的被检测核酸的微粒（103）通过在基板（101）上排列的微结构（102）布置。

10. 发明申请

US20130309675A1 Microparticle having single-molecule nucleic acid probe, method for producing same, method for analyzing nucleic acid using same, device for nucleic acid analysis, and apparatus for analyzing nucleic acid 有权
标题翻译：具有单分子核酸探针的微粒，其制备方法，使用其的核酸分析方法，核酸分析装置和核酸分析装置
公开(公告)号：US20130309675A1
公开(公告)日：2013-11-21
申请号：US13981983
申请日：2012-01-26
申请人： Toshiro Saito , Kazumichi Imai , Takayuki Obara , Eri Tarasawa
发明人： Toshiro Saito , Kazumichi Imai , Takayuki Obara , Eri Tarasawa
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6806 , C12Q1/6834 , C12Q1/6876 , C12Q2525/131 , C12Q2525/197 , C12Q2535/122 , C12Q2537/125 , C12Q2537/159 , C12Q2563/155
摘要： A microparticle having a probe molecule able to capture a specific nucleic acid group to be analyzed is used to extract only the specific nucleic acid group to be analyzed from a nucleic acid sample and the microparticle is thereafter directly immobilized on a smooth plate, whereby a device for nucleic acid analysis is rapidly prepared. Immobilizing a single capture probe molecule onto an individual microparticle in advance and forming, at regular positions on the smooth substrate, an adhesion pad on which a functional group that binds to the microparticle has been introduced makes it possible to readily and rapidly prepare the device for nucleic analysis, where the nucleic acid sample to be analyzed is arranged molecule by molecule in a lattice shape on the smooth substrate.
摘要翻译：使用具有能够捕获要分析的特定核酸组的探针分子的微粒从核酸样品中仅提取待分析的特异性核酸组，然后将微粒直接固定在光滑平板上，用于核酸分析快速准备。将单个捕获探针分子预先固定在单个微粒上，并且在光滑基底上的规则位置处形成粘附有与微粒结合的官能团的粘合垫，使得可以容易且快速地制备用于核酸分析，其中要分析的核酸样品以平滑基底上的格子状分子排列。

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