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1. 发明授权

US5057438A Electrophoretic antigen-antibody determination with laminate of multiple membranes 失效
标题翻译：电泳抗原 - 抗体用多层膜叠层测定
公开(公告)号：US5057438A
公开(公告)日：1991-10-15
申请号：US31665
申请日：1987-03-30
申请人： Kazumichi Imai , Daizo Tokinaga , Teruaki Kobayashi , Kenji Yasuda , Keiichi Nagai , Satoshi Takahashi
发明人： Kazumichi Imai , Daizo Tokinaga , Teruaki Kobayashi , Kenji Yasuda , Keiichi Nagai , Satoshi Takahashi
IPC分类号： G01N33/561
CPC分类号： G01N33/561 , Y10S436/809 , Y10S436/824
摘要： Determination of a plurality of species of antibodies or antigens is attained by a method which comprises forming a plurality of different kinds of reaction membranes each having a different species of antibody or antigen on an electrophoretic carrier, superposing these reaction membranes, optionally superposing a filter on the laminate of reaction membranes, inserting the laminate in an electrolyte, adding a plurality of different species of antigens or antibodies corresponding to the plurality of species of antibodies or antigens supported in the aforementioned reaction membranes, electrophoretically moving the added antigens or antibodies through the electrolyte and enabling them to react with the antibodies or antigens supported on the reaction membranes, and measuring the concentrations of either the antigens or antibodies resulting from the reaction or the antibodies or antigens supported in an unreacted form on the reaction membranes.
摘要翻译：通过包括在电泳载体上形成多个不同种类的抗体或抗原的不同种类的反应膜，叠加这些反应膜，任选地将过滤器叠加在一起的方法来实现多种抗体或抗原的测定反应膜的层压体，将层压体插入电解质中，加入与上述反应膜中支持的多种抗体或抗原相对应的多种不同种类的抗原或抗体，通过电解质电泳移动加入的抗原或抗体并使其能够与支持在反应膜上的抗体或抗原反应，并测量由反应产生的抗原或抗体的浓度，或反应膜上以未反应形式负载的抗体或抗原的浓度。

2. 发明授权

US4824778A Immunoassay for measuring the concentration of antigen in a sample 失效
标题翻译：用于测量样品中抗原浓度的免疫测定
公开(公告)号：US4824778A
公开(公告)日：1989-04-25
申请号：US867554
申请日：1986-05-28
申请人： Keiichi Nagai , Daizo Tokinaga , Kazumichi Imai , Kenji Yasuda , Satoshi Takahashi , Teruaki Kobayashi
发明人： Keiichi Nagai , Daizo Tokinaga , Kazumichi Imai , Kenji Yasuda , Satoshi Takahashi , Teruaki Kobayashi
IPC分类号： G01N33/543 , G01N33/561 , G01N33/53
CPC分类号： G01N33/561 , Y10S435/968
摘要： An immunoassay comprisingimmobilizing antibody in a matrix for electrophoresis;immobilizing antigen in a measurement sample by subjecting the same to antigen antibody reaction with the above-mentioned immobilized antibody by a procedure of moving the antigen by electrophoresis;either moving labeled antibody to the above-mentioned immobilized antigen by electrophoresis to react the same with the immobilized antigen, or moving labeled antigen to the unreacted portion of the above-mentioned immobilized antibody by electrophoresis to react the same with the unreacted portion; andmeasuring the concentration of antigen in the sample, characterized byusing as a label for the labeled antibody or the labeled antigen an enzyme capable of coverting a substrate into a fluorescent substance,moving the substrate convertible into a fluorescent substance by said enzyme by electrophoresis,reacting the substrate with the label enzyme to convert the same into a fluorescent substance, andmeasuring the concentration of the fluorescent substance in the electrolyte solution.
摘要翻译：免疫测定法，其包括将抗体固定在用于电泳的基质中; 通过使抗原通过电泳移动的方法，将抗原固定在测量样品中，使其与上述固定化抗体进行抗原抗体反应; 通过电泳将标记抗体移动到上述固定化抗原上，使其与固定化抗原反应，或通过电泳将标记的抗原移动到上述固定化抗体的未反应部分，使其与未反应部分反应; 测定样品中的抗原浓度，其特征在于，将标记抗体或标记抗原的标记作为能够将底物覆盖成荧光物质的酶，通过电泳将所述酶转化为荧光物质，使底物与标记酶反应，将其转化为荧光物质，并测量电解质溶液中荧光物质的浓度。

3. 发明授权

US5824481A DNA analyzing method 失效
标题翻译： DNA分析方法
公开(公告)号：US5824481A
公开(公告)日：1998-10-20
申请号：US812698
申请日：1997-03-06
申请人： Hideki Kambara , Kazunori Okano , Satoshi Takahashi , Keiichi Nagai , Kazuko Kawamoto , Hiroko Furuyama
发明人： Hideki Kambara , Kazunori Okano , Satoshi Takahashi , Keiichi Nagai , Kazuko Kawamoto , Hiroko Furuyama
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6846
摘要： A DNA analyzing method which bonds a first oligomer of known base sequence to a DNA fragment obtained by digesting a DNA sample with a restrictive enzyme. The oligomer and DNA fragment are hybridized to other oligomers which have the sequences of all combinations of the types of bases within the length of several bases following the known base sequence. The presence or absence of hybridization or complementary DNA strand extension is determined and identifies the DNA fragment terminal sequence from this result. The DNA fragments are then fractionated and analyzed to determine the sequence. This DNA analyzing method provides an effective analysis of mixtures of long DNAs or DNA fragments.
摘要翻译：将已知碱基序列的第一寡聚体与限制性酶消化DNA样品获得的DNA片段结合的DNA分析方法。寡聚体和DNA片段与其它寡核苷酸杂交，其具有在已知碱基序列之后的几个碱基长度内的碱基类型的所有组合的序列。确定是否存在杂交或互补DNA链延伸，并从该结果鉴定DNA片段末端序列。然后将DNA片段分级并分析以确定序列。该DNA分析方法提供了长DNA或DNA片段的混合物的有效分析。

4. 发明授权

US5356776A DNA measuring method 失效
标题翻译： DNA测定方法
公开(公告)号：US5356776A
公开(公告)日：1994-10-18
申请号：US942470
申请日：1992-09-09
申请人： Hideki Kambara , Kazunori Okano , Satoshi Takahashi , Keiichi Nagai , Tetsuo Nishikawa
发明人： Hideki Kambara , Kazunori Okano , Satoshi Takahashi , Keiichi Nagai , Tetsuo Nishikawa
IPC分类号： C12M1/00 , C12Q1/68 , G01N27/447 , G01N33/53 , G01N33/58 , C12Q1/70
CPC分类号： G01N27/44726 , C12Q1/68 , G01N27/447 , Y10T436/143333
摘要： DNA molecule length can be measured with high precision and efficiency by 1) using such means as electrophoresis gel migration to orient a DNA molecule having a fluorescence label at both its termini into a straight line by its passing through a migration path having in a portion of it an area not more than several micrometers in diameter, detecting the fluorescence label at both the termini at a predetermined location and measuring the interval between the detection of the fluorescence coming from one terminus and that of the fluorescence from the other or by 2) a DNA molecule bound to a fluorescence label at one terminus and to a particle at the other being led as a whole by such means as electric field application into an aperture smaller in diameter than the particle, leaving the particle fixed at the mouth of the aperture to stretch the DNA molecule and detecting the fluorescence position to measure the distance between the bound particle and the bound fluorescence label.
摘要翻译：可以通过以下方式测量DNA分子长度：1）使用电泳凝胶迁移的方式，通过其经过一部分迁移路径将具有两端的荧光标记的DNA分子定向成直线它是直径不超过几微米的区域，在预定位置的两个末端检测荧光标记，并测量从一个末端检测荧光和来自另一个末端的荧光检测之间的间隔，或者通过2）a 在一个末端与一个荧光标记结合的DNA分子和另一个的一个粒子整体通过电场施加到比颗粒直径更小的孔隙中，将颗粒固定在孔的口处拉伸DNA分子并检测荧光位置以测量结合的颗粒和结合的荧光标记之间的距离。

5. 发明授权

US5650274A DNA analyzing method 失效
标题翻译： DNA分析方法
公开(公告)号：US5650274A
公开(公告)日：1997-07-22
申请号：US263663
申请日：1994-06-22
申请人： Hideki Kambara , Kazunori Okano , Satoshi Takahashi , Keiichi Nagai , Kazuko Kawamoto , Hiroko Furuyama
发明人： Hideki Kambara , Kazunori Okano , Satoshi Takahashi , Keiichi Nagai , Kazuko Kawamoto , Hiroko Furuyama
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34 , C12Q1/70
CPC分类号： C12Q1/6869 , C12Q1/6846
摘要： A DNA analyzing method comprising ligating the oligomer of a known base sequence to the DNA fragment obtained by digesting a sample with a restriction enzyme, hybridizing an oligomer and DNA fragment using oligomers which have the sequences of all combinations of the types of the bases within the length of several bases following the known base sequence, checking the presence or absence of hybridization or complementary DNA strand extension, identifying the DNA fragment terminal sequence from this result, and fractionating the DNA fragments and analyzing them. This DNA analyzing method provides an effective analysis of mixtures of long DNAs or DNA fragments.
摘要翻译：一种DNA分析方法，包括将已知碱基序列的寡聚物与通过用限制酶消化样品获得的DNA片段连接，使用寡核苷酸与低聚物和DNA片段杂交，所述寡聚体具有碱基类型的所有组合的序列根据已知碱基序列的几个碱基的长度，检查杂交或互补DNA链延伸的存在或不存在，从该结果鉴定DNA片段末端序列，并分离DNA片段并分析它们。该DNA分析方法提供了长DNA或DNA片段的混合物的有效分析。

6. 发明申请

US20100173287A1 METHOD OF ANALYZING NUCLEIC ACID 审中-公开
标题翻译：分析核酸的方法
公开(公告)号：US20100173287A1
公开(公告)日：2010-07-08
申请号：US12090701
申请日：2006-03-07
申请人： Yukie Nakashima , Keiichi Nagai
发明人： Yukie Nakashima , Keiichi Nagai
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q1/6839 , C12Q2537/119 , C12Q2521/501
摘要： According to the present invention, stable amplification of a small amount of nucleic acid and analysis of the same with good sensitivity can be realized by improving efficiency of hybridization primers or probes with a probe. Specifically, the present invention relates to a method of analyzing nucleic acid comprising: a step of hybridizing at least one type of a first probe comprising a 1st sequence complementary to one strand of double-strand nucleic acid, a 2nd sequence complementary to the other strand thereof with the double-strand nucleic acid, and a 3rd sequence that binds the 1st sequence and the 2nd sequence; and a step of hybridizing at least one type of a second probe with the double-strand nucleic acid.
摘要翻译：根据本发明，通过用探针提高杂交引物或探针的效率，可以实现少量核酸的稳定扩增及其灵敏度的分析。具体地说，本发明涉及分析核酸的方法，包括：将至少一种类型的第一探针杂交的步骤，所述第一探针包含与一条双链核酸互补的第一序列，与另一条链互补的第二序列与第二序列和第二序列结合的第三序列; 以及使至少一种类型的第二探针与双链核酸杂交的步骤。

7. 发明申请

US20070020637A1 Full-length cDNA 审中-公开
标题翻译：全长cDNA
公开(公告)号：US20070020637A1
公开(公告)日：2007-01-25
申请号：US10760320
申请日：2004-01-21
申请人： Takao Isogai , Tomoyasu Sugiyama , Tetsuji Otsuki , Al Wakamatsu , Hiroyuki Sato , Shizuko Ishii , Junichi Yamamoto , Yuko Isono , Keiichi Nagai , Ryotaro Irie
发明人： Takao Isogai , Tomoyasu Sugiyama , Tetsuji Otsuki , Al Wakamatsu , Hiroyuki Sato , Shizuko Ishii , Junichi Yamamoto , Yuko Isono , Keiichi Nagai , Ryotaro Irie
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C07K14/47
摘要： Novel full-length cDNAs are provided. 2,495 cDNA derived from human have been isolated. The full-length nucleotide sequences of the cDNA and amino acid sequences encoded by the nucleotide sequences have been determined. Because the cDNA of the present invention are full-length and contain the translation start site, they provide information useful for analyzing the functions of the polypeptide.
摘要翻译：提供新的全长cDNA。已经分离出来自人的2,495个cDNA。已经确定了由核苷酸序列编码的cDNA和氨基酸序列的全长核苷酸序列。因为本发明的cDNA是全长的并含有翻译起始位点，所以它们提供了可用于分析多肽功能的信息。

8. 发明申请

US20050250144A1 Novel genes encoding protein kinase/protein phosphatase 审中-公开
标题翻译：编码蛋白激酶/蛋白磷酸酶的新基因
公开(公告)号：US20050250144A1
公开(公告)日：2005-11-10
申请号：US11109156
申请日：2005-04-19
申请人： Toshio Ota , Takao Isogai , Tetsuo Nishikawa , Koji Hayashi , Kaoru Otsuka , Jun-Ichi Yamamoto , Shizuko Ishii , Tomoyasu Sugiyama , Ai Wakamatsu , Keiichi Nagai , Tetsuji Otsuki , Shin-Ichi Funahashi , Chiaki Senoo , Jin-Ichi Nezu
发明人： Toshio Ota , Takao Isogai , Tetsuo Nishikawa , Koji Hayashi , Kaoru Otsuka , Jun-Ichi Yamamoto , Shizuko Ishii , Tomoyasu Sugiyama , Ai Wakamatsu , Keiichi Nagai , Tetsuji Otsuki , Shin-Ichi Funahashi , Chiaki Senoo , Jin-Ichi Nezu
IPC分类号： A61K38/00 , C07K14/47 , C12N1/15 , C12N1/19 , C12N1/21 , C12N15/12 , C12Q1/68 , C07H21/04 , C12N9/12 , C12N9/16 , C12N15/09 , C12P21/06
CPC分类号： C07K14/4702 , A61K38/00 , A61K2039/505 , A61K2039/53 , C07K14/47 , C12N2799/021
摘要： Selection of clones having the kinase and/or phosphatase-like structure from clones which had been isolated and the structures thereof had been determined in the Helix Research Institute (helix clones; Japanese Patent Application No. 2000-183767) was conducted. Two novel genes were provided by carrying out homology search for all the helix clones by using the amino acid sequences of known kinases and phosphatases as queries. The genes are expected to be involved in intracellular signal transduction. The physiological functions of the inventive genes can be tested by using reporter gene assay systems capable of detecting signal transduction. The proteins of the present invention are useful as target molecules in drug discovery and in the development of new pharmaceuticals.
摘要翻译：已经在螺旋研究所（螺旋克隆，日本专利申请2000-183767）中确定了已经分离的克隆的克隆和/或磷酸酶样结构的克隆及其结构的选择。通过使用已知的激酶和磷酸酶的氨基酸序列作为查询，通过对所有螺旋克隆进行同源性检索来提供两个新基因。这些基因预期参与细胞内信号转导。可以通过使用能够检测信号转导的报告基因测定系统来测试本发明基因的生理功能。本发明的蛋白质可用作药物发现和开发新药物中的靶分子。

9. 发明授权

US06531044B1 Capillary array electrophoresis apparatus 有权
标题翻译：毛细管阵列电泳仪
公开(公告)号：US06531044B1
公开(公告)日：2003-03-11
申请号：US09425064
申请日：1999-10-25
申请人： Takashi Anazawa , Keiichi Nagai
发明人： Takashi Anazawa , Keiichi Nagai
IPC分类号： G01N2726
CPC分类号： G01N27/44782 , G01N27/44721
摘要： The electrophoresis apparatus includes plural capillaries for separating fluorephore-labeled samples by electrophoresis, fluorescence detecting parts provided in a part of these capillaries arranged in the same place for detecting a fluorescence emitted by fluorephore labels when a part of the plural capillaries is scanned and irradiated by a laser beam, and a fluorescence detection system for detecting this fluorescence. The fluorescence detecting parts are scanned and repeatedly irradiated by the laser bean where a scanning period of the fluorescence detecting parts by the laser bean is t1, and the fluorescence is detected by the fluorescence detecting system where an acquisition time of fluorescence signal is t2 (t1≦t2). The laser bean from a laser source is narrowly converged by a light collecting lens, and a galvanomirror is rotated in a rotation directional of the galvanomirror around the rotation axis of the galvanomirror so as to repeatedly scan the fluorescence detecting parts.
摘要翻译：电泳装置包括多个毛细管，用于通过电泳分离荧光标记的样品，荧光检测部分设置在这些毛细管的一部分中，布置在相同的位置，用于当多个毛细血管的一部分被扫描和照射时，用于检测由荧光标记物发出的荧光激光束和用于检测该荧光的荧光检测系统。荧光检测部件被激光束的荧光检测部的扫描期间为t1的激光束扫描并重复照射，荧光检测系统通过荧光检测系统检测荧光，其中荧光信号的获取时间为t2（t1 <= t2）。来自激光源的激光束由聚光透镜狭窄地收敛，并且电流镜以电流镜的旋转方向围绕电流镜的旋转轴线旋转，以便重复扫描荧光检测部分。

10. 发明授权

US5307148A Fluorescence detection type electrophoresis apparatus 失效
标题翻译：荧光检测型电泳仪
公开(公告)号：US5307148A
公开(公告)日：1994-04-26
申请号：US684113
申请日：1991-04-12
申请人： Hideki Kambara , Keiichi Nagai , Tamotu Simada , Tetsuo Nishikawa , Tomoaki Sumitani
发明人： Hideki Kambara , Keiichi Nagai , Tamotu Simada , Tetsuo Nishikawa , Tomoaki Sumitani
IPC分类号： G01N21/64 , G01N27/447
CPC分类号： G01N21/645 , G01N27/44721 , G01N2021/6441
摘要： A multicolor fluorescence detection type electrophoresis apparatus comprises an electrophoretic device having migration lanes in which fragment groups specifically labeled by fluorophores of different light emission peak wavelengths are mixed, added, and migrated. It identifies and detects the fragment groups. It uses laser beams of different wavelengths for exciting the fluorophores in the migration lanes. The laser beams are irradiated to separate positions on the migration lanes for detection of a few kinds of fluorophores at irradiation positions. For the purpose, multicolor optical filters are arranged near the irradiation positions to identify and detect lights emitted from the fluorophores excited by wavelength at every irradiation position.
摘要翻译：多色荧光检测型电泳装置包括具有迁移通道的电泳装置，其中由不同发光峰波长的荧光团特异性标记的片段组被混合，添加和迁移。它识别和检测片段组。它使用不同波长的激光束激发迁移通道中的荧光团。将激光束照射到迁移道上的分离位置，以便在照射位置检测几种荧光团。为此，在照射位置附近设置多色滤光片，以识别和检测在每个照射位置由被波长激发的荧光团发出的光。

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