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    • 3. 发明授权
    • Materials and methods for the alteration of enzyme and acetyl CoA levels in plants
    • 用于改变植物中酶和乙酰CoA水平的材料和方法
    • US07524678B2
    • 2009-04-28
    • US11167856
    • 2005-06-27
    • Basil J. NikolauEve S. WurteleDavid J. OliverPatrick S. SchnableTsui-Jung Wen
    • Basil J. NikolauEve S. WurteleDavid J. OliverPatrick S. SchnableTsui-Jung Wen
    • C12N5/10
    • C12N15/1137C12N9/0008C12N9/0051C12N9/1029C12N9/16C12N9/88C12N9/93C12N15/8243
    • The present invention provides nucleic acid and amino acid sequences of acetyl CoA synthetase (ACS), plastidic pyruvate dehydrogenase (pPDH), ATP citrate lyase (ACL), Arabidopsis pyruvate decarboxylase (PDC), and Arabidopsis aldehyde dehydrogenase (ALDH), specifically ALDH-2 and ALDH-4. The present invention also provides a recombinant vector comprising a nucleic acid sequence encoding one of the aforementioned enzymes, an antisense sequence thereto or a ribozyme therefor, a cell transformed with such a vector, antibodies to the enzymes, a plant cell, a plant tissue, a plant organ or a plant in which the level of an enzyme has been altered, and a method of producing such a plant cell, plant tissue, plant organ or plant. Desirably, alteration of the level of enzyme results in an alteration of the level of acetyl CoA in the plant cell, plant tissue, plant organ or plant. In addition, the present invention provides a recombinant vector comprising an antisense sequence of a nucleic acid sequence encoding pyruvate decarboxylase (PDC), the E1α subunit of pPDH, the E1β subunit of pPDH, the E2 subunit of pPDH, mitochondrial pyruvate dehydrogenase (mtPDH) or aldehyde dehydrogenase (ALDH) or a ribozyme that can cleave an RNA molecule encoding PDC, E1α pPDH, E1β pPDH, E2 pPDH, mtPDH or ALDH.
    • 本发明提供乙酰辅酶A合成酶(ACS),质体丙酮酸脱氢酶(pPDH),ATP柠檬酸裂合酶(ACL),拟南芥丙酮酸脱羧酶(PDC)和拟南芥醛脱氢酶(ALDH),特别是ALDH- 2和ALDH-4。 本发明还提供了一种重组载体,其包含编码上述酶之一的核酸序列,其上的反义序列或其核酶,用该载体转化的细胞,对酶的抗体,植物细胞,植物组织, 其中酶的水平已被改变的植物器官或植物,以及生产这种植物细胞,植物组织,植物器官或植物的方法。 理想地,酶水平的改变导致植物细胞,植物组织,植物器官或植物中乙酰CoA水平的改变。 此外,本发明提供了包含编码丙酮酸脱羧酶(PDC),pPDH的E1alpha亚基,pPDH的E1beta亚基,pPDH的E2亚基,线粒体丙酮酸脱氢酶(mtPDH)的核酸序列的反义序列的重组载体, 或醛脱氢酶(ALDH)或可以切割编码PDC,E1alpha pPDH,E1beta pPDH,E2pPDH,mtPDH或ALDH的RNA分子的核酶。
    • 7. 发明授权
    • Screw threaded locking bolt assembly
    • 螺纹锁紧螺栓总成
    • US5304021A
    • 1994-04-19
    • US956858
    • 1992-10-02
    • David J. OliverDavid P. Halliday
    • David J. OliverDavid P. Halliday
    • F16B39/02F16B39/10F16B39/24
    • F16B39/02
    • Locking bolt assembly comprises a bush (9) fixed in use with respect to a structure to which a bolt of the assembly is to be engaged and a socket (1) carried on the bush (9) and provided with locking means (6) for preventing rotation of the socket (1) on the bush (9) but permitting limited longitudinal movement thereon. The bolt is threaded and has a head (3) dimensioned for rotational locking engagement within socket (1). A coil spring (7) urges the head (3) and socket (1) into rotational locking engagement. In use the socket (1) may be moved longitudinally with respect to said bush (9) against the action of the spring (7) from a position in which the head (3) and the socket (1) are radically locked, to a position in which the head (3) is freed for rotation and engagement with a co-operating nut fixed to the structure.
    • 锁定螺栓组件包括相对于组件的螺栓将被接合的结构固定在使用中的衬套(9)和承载在衬套(9)上的插座(1)并且设置有锁定装置(6),用于 防止插座(1)旋转到衬套(9)上,但允许其上的纵向移动受限。 螺栓是螺纹的并且具有用于在插座(1)内旋转锁定接合的头部(3)。 螺旋弹簧(7)促使头部(3)和插座(1)旋转锁定接合。 在使用中,插座(1)可以克服弹簧(7)从头部(3)和插座(1)被自由锁定的位置相对于所述衬套(9)纵向移动到 头部(3)被释放以与固定到结构的辅助螺母旋转和接合的位置。