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1. 发明授权

US09304070B2 All-in-one sample preparation device and method 有权
公开(公告)号：US09304070B2
公开(公告)日：2016-04-05
申请号：US13534570
申请日：2012-06-27
申请人： Christopher A. Scott , Timothy K. Nadler , Kurt Greenizen , Louis Bonhomme , Sara D. Gutierrez , Masaharu Mabuchi , David Briggs , Phillip Clark , Ralph T. Scaduto
发明人： Christopher A. Scott , Timothy K. Nadler , Kurt Greenizen , Louis Bonhomme , Sara D. Gutierrez , Masaharu Mabuchi , David Briggs , Phillip Clark , Ralph T. Scaduto
IPC分类号： B01L3/00 , B01D63/08 , B01D25/22 , B01D21/26 , G01N1/34 , G01N1/40 , B01D61/18 , B01D63/06 , B01D63/16 , C07K1/22 , C07K1/16
CPC分类号： G01N1/34 , B01D61/18 , B01D63/06 , B01D63/16 , B01D2315/02 , B01L3/5021 , B01L3/5085 , B01L2300/0618 , B01L2300/0681 , C07K1/16 , C07K1/22 , G01N1/405 , G01N1/4077 , G01N2001/4088 , Y10T436/25375
摘要： Sample preparation device that allows for a complete bind, wash, elute, buffer-exchange and concentration process to be carried out without sample transfer between multiple devices. The device includes a reservoir, a column for holding chromatography media, a holder region for holding a filtration device, and an outlet. The filtration device plugs into the holder region of the centrifugal device, and the assembly can be placed in an optional holder. The assembly, with or without the optional holder, can be placed in a conventional centrifuge tube for centrifugation. The entire bind, wash, elute, buffer exchange and concentration steps can be carried out with the apparatus without any pipette transfers (and the associated sample losses. The sample preparation device also can be used for binding and washing steps, in which case the filtration device is not needed, and for buffer exchange and concentration steps, in which case the media is not needed.

2. 发明申请

US20130017620A1 All-In-One Sample Preparation Device And Method 有权
标题翻译：一体式样品制备装置及方法
公开(公告)号：US20130017620A1
公开(公告)日：2013-01-17
申请号：US13534570
申请日：2012-06-27
申请人： Christopher A. Scott , Timothy K. Nadler , Kurt Greenizen , Louis Bonhomme , Sara D. Gutierrez , Masaharu Mabuchi , David Briggs , Phillip Clark , Ralph T. Scaduto
发明人： Christopher A. Scott , Timothy K. Nadler , Kurt Greenizen , Louis Bonhomme , Sara D. Gutierrez , Masaharu Mabuchi , David Briggs , Phillip Clark , Ralph T. Scaduto
IPC分类号： G01N1/34
CPC分类号： G01N1/34 , B01D61/18 , B01D63/06 , B01D63/16 , B01D2315/02 , B01L3/5021 , B01L3/5085 , B01L2300/0618 , B01L2300/0681 , C07K1/16 , C07K1/22 , G01N1/405 , G01N1/4077 , G01N2001/4088 , Y10T436/25375
摘要： Sample preparation device that allows for a complete bind, wash, elute, buffer-exchange and concentration process to be carried out without sample transfer between multiple devices. The device includes a reservoir, a column for holding chromatography media, a holder region for holding a filtration device, and an outlet. The filtration device plugs into the holder region of the centrifugal device, and the assembly can be placed in an optional holder. The assembly, with or without the optional holder, can be placed in a conventional centrifuge tube for centrifugation. The entire bind, wash, elute, buffer exchange and concentration steps can be carried out with the apparatus without any pipette transfers (and the associated sample losses. The sample preparation device also can be used for binding and washing steps, in which case the filtration device is not needed, and for buffer exchange and concentration steps, in which case the media is not needed.
摘要翻译：允许完全结合，洗涤，洗脱，缓冲交换和浓缩过程的样品制备装置，在多个装置之间没有样品转移的情况下进行。该装置包括储存器，用于保持色谱介质的柱，用于保持过滤装置的保持器区域和出口。过滤装置插入到离心装置的保持器区域中，并且组件可以放置在可选的保持器中。具有或不具有任选的保持器的组件可以放置在用于离心的常规离心管中。整个结合，洗涤，洗脱，缓冲液交换和浓缩步骤可以在没有任何移液管转移（和相关样品损失）的情况下进行，样品制备装置也可用于结合和洗涤步骤，在这种情况下，过滤设备不需要，并且用于缓冲区交换和集中步骤，在这种情况下不需要介质。

3. 发明授权

US08652421B2 Immunoassay product and process 有权
公开(公告)号：US08652421B2
公开(公告)日：2014-02-18
申请号：US11582599
申请日：2006-10-18
申请人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
发明人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
IPC分类号： G01N33/50 , C12M1/40
CPC分类号： B01L3/5025 , B01L3/50255 , B01L3/50853 , B01L2200/0642 , B01L2300/043 , B01L2300/069 , B01L2300/0829 , B01L2400/0487
摘要： The invention is directed to an apparatus useful in conducting detection of compounds on blotting membranes. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and optionally a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane and the support layer is a grid or sintered porous material. The distributor and support are held together to form an envelope around the membrane(s). The use of a hinge, clips and other such devices is preferred in doing so.

4. 发明授权

US08652422B2 Immunoassay product and process 有权
标题翻译：免疫测定产品和方法
公开(公告)号：US08652422B2
公开(公告)日：2014-02-18
申请号：US13078290
申请日：2011-04-01
申请人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
发明人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
IPC分类号： G01N33/50 , C12M1/40
CPC分类号： B01L3/5025 , B01L3/50255 , B01L3/50853 , B01L2200/0642 , B01L2300/043 , B01L2300/069 , B01L2300/0829 , B01L2400/0487
摘要： The invention is directed to an apparatus useful in conducting detection of compounds on blotting membranes. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and optionally a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane and the support layer is a grid or sintered porous material. The distributor and support are held together to form an envelope around the membrane(s). The use of a hinge, clips and other such devices is preferred in doing so.
摘要翻译：本发明涉及一种用于在印迹膜上进行化合物检测的装置。该装置包括多个层，包括印迹膜下面的多孔支撑层，印迹膜上方的流量分布器，以及任选的流量分配器上的孔，以将液体包含到期望的区域并允许降低这种液体的起始体积。优选地，流动分配器是非结合或低结合的亲水性多孔膜，例如0.22微米的膜，并且支撑层是格栅或烧结的多孔材料。分配器和支撑件保持在一起以在膜周围形成一个信封。在这样做时优选使用铰链，夹子和其它这样的装置。

5. 发明申请

US20070243628A1 Immunoassay product and process 有权
标题翻译：免疫测定产品和方法
公开(公告)号：US20070243628A1
公开(公告)日：2007-10-18
申请号：US11582727
申请日：2006-10-18
申请人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
发明人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
IPC分类号： G01N33/53 , B01J19/00
CPC分类号： G01N33/5302 , B01L3/50255 , B01L2400/0487 , G01N33/54366 , G01N33/54393 , G01N33/561 , Y10T436/2575
摘要： A rapid, efficient and convenient method to detect one or more biological entities on a blotting membrane is provided. The detection can relate to the position, nature or amount of the biological substance on one or more membranes. The invention method involves a pressure assisted regiment (such as vacuum or positive gas pressure) for the supply and removal of reagents and permits washing of the contaminants from substances embedded in the membrane to be detected using very low volumes of liquid. This method enables completion of the blocking, washing and antibody binding steps in about 30 minutes without comprising blot quality. In another aspect, the invention is directed to an apparatus useful in conducting the method of the invention. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane.
摘要翻译：提供了一种快速，有效和方便地检测印迹膜上的一个或多个生物实体的方法。检测可以涉及一个或多个膜上的生物物质的位置，性质或数量。本发明方法包括用于供应和去除试剂的压力辅助团（例如真空或正气压），并且允许使用非常低体积的液体从嵌入在待检测膜中的物质洗涤污染物。该方法能够在约30分钟内完成阻断，洗涤和抗体结合步骤，而不包括印迹质量。另一方面，本发明涉及可用于实施本发明方法的装置。该装置由多个层组成，包括印迹膜下方的多孔支撑层，印迹膜上方的流动分布器以及流量分配器上的孔，以将液体容纳到所需区域并允许较低的开始这种液体的体积。优选地，流动分配器是非结合或低结合的亲水性多孔膜，例如0.22微米的膜。

6. 发明申请

US20070098601A1 Immunoassay product and process 有权
标题翻译：免疫测定产品和方法
公开(公告)号：US20070098601A1
公开(公告)日：2007-05-03
申请号：US11582599
申请日：2006-10-18
申请人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
发明人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
IPC分类号： B01L9/00
CPC分类号： B01L3/5025 , B01L3/50255 , B01L3/50853 , B01L2200/0642 , B01L2300/043 , B01L2300/069 , B01L2300/0829 , B01L2400/0487
摘要： The invention is directed to an apparatus useful in conducting detection of compounds on blotting membranes. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and optionally a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane and the support layer is a grid or sintered porous material. The distributor and support are held together to form an envelope around the membrane(s). The use of a hinge, clips and other such devices is preferred in doing so.
摘要翻译：本发明涉及一种用于在印迹膜上进行化合物检测的装置。该装置包括多个层，包括印迹膜下面的多孔支撑层，印迹膜上方的流量分布器，以及任选的流量分配器上的孔，以将液体包含到期望的区域并允许降低这种液体的起始体积。优选地，流动分配器是非结合或低结合的亲水性多孔膜，例如0.22微米的膜，并且支撑层是格栅或烧结的多孔材料。分配器和支撑件保持在一起以在膜周围形成一个信封。在这样做时优选使用铰链，夹子和其它这样的装置。

7. 发明申请

US20110038757A1 IMMUNOASSAY PRODUCT AND PROCESS 有权
标题翻译：免疫产品和过程
公开(公告)号：US20110038757A1
公开(公告)日：2011-02-17
申请号：US12911407
申请日：2010-10-25
申请人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
发明人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
IPC分类号： G01N33/48
CPC分类号： B01L3/5025 , B01L3/50255 , B01L3/50853 , B01L2200/0642 , B01L2300/043 , B01L2300/069 , B01L2300/0829 , B01L2400/0487
摘要： The invention is directed to an apparatus useful in conducting detection of compounds on blotting membranes. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and optionally a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane and the support layer is a grid or sintered porous material. The distributor and support are held together to form an envelope around the membrane(s). The use of a hinge, clips, and other such devices is preferred in doing so.
摘要翻译：本发明涉及一种用于在印迹膜上进行化合物检测的装置。该装置包括多个层，包括印迹膜下面的多孔支撑层，印迹膜上方的流量分布器，以及任选的流量分配器上的孔，以将液体包含到期望的区域并允许降低这种液体的起始体积。优选地，流动分配器是非结合或低结合的亲水性多孔膜，例如0.22微米的膜，并且支撑层是格栅或烧结的多孔材料。分配器和支撑件保持在一起以在膜周围形成一个信封。在这样做时优选使用铰链，夹子和其它这样的装置。

8. 发明授权

US08557600B2 Immunoassay product and process 有权
标题翻译：免疫测定产品和方法
公开(公告)号：US08557600B2
公开(公告)日：2013-10-15
申请号：US11582727
申请日：2006-10-18
申请人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
发明人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
IPC分类号： G01N1/10
CPC分类号： G01N33/5302 , B01L3/50255 , B01L2400/0487 , G01N33/54366 , G01N33/54393 , G01N33/561 , Y10T436/2575
摘要： A rapid, efficient and convenient method to detect one or more biological entities on a blotting membrane is provided. The detection can relate to the position, nature or amount of the biological substance on one or more membranes. The invention method involves a pressure assisted regiment (such as vacuum or positive gas pressure) for the supply and removal of reagents and permits washing of the contaminants from substances embedded in the membrane to be detected using very low volumes of liquid. This method enables completion of the blocking, washing and antibody binding steps in about 30 minutes without comprising blot quality. In another aspect, the invention is directed to an apparatus useful in conducting the method of the invention. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane.
摘要翻译：提供了一种快速，有效和方便地检测印迹膜上的一个或多个生物实体的方法。检测可以涉及一个或多个膜上的生物物质的位置，性质或数量。本发明方法包括用于供应和去除试剂的压力辅助团（例如真空或正气压），并且允许使用非常低体积的液体从嵌入在待检测膜中的物质洗涤污染物。该方法能够在约30分钟内完成封闭，洗涤和抗体结合步骤，而不包括印迹质量。另一方面，本发明涉及可用于实施本发明方法的装置。该装置由多个层组成，包括印迹膜下方的多孔支撑层，印迹膜上方的流动分布器以及流量分配器上的孔，以将液体容纳到所需区域并允许较低的开始这种液体的体积。优选地，流动分配器是非结合或低结合的亲水性多孔膜，例如0.22微米的膜。

9. 发明授权

US08460618B2 Immunoassay product and process 有权
标题翻译：免疫测定产品和方法
公开(公告)号：US08460618B2
公开(公告)日：2013-06-11
申请号：US12911407
申请日：2010-10-25
申请人： Masaharu Mabuchi , Hiroko Ykohama , Marc Emerick , Phillip Clark , Kurt Greenizen
发明人： Masaharu Mabuchi , Hiroko Ykohama , Marc Emerick , Phillip Clark , Kurt Greenizen
IPC分类号： G01N33/50 , C12M1/40
CPC分类号： B01L3/5025 , B01L3/50255 , B01L3/50853 , B01L2200/0642 , B01L2300/043 , B01L2300/069 , B01L2300/0829 , B01L2400/0487
摘要： The invention is directed to an apparatus useful in conducting detection of compounds on blotting membranes. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and optionally a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane and the support layer is a grid or sintered porous material. The distributor and support are held together to form an envelope around the membrane(s). The use of a hinge, clips and other such devices is preferred in doing so.
摘要翻译：本发明涉及一种用于在印迹膜上进行化合物检测的装置。该装置包括多个层，包括印迹膜下面的多孔支撑层，印迹膜上方的流量分布器，以及任选的流量分配器上的孔，以将液体包含到期望的区域并允许降低这种液体的起始体积。优选地，流动分配器是非结合或低结合的亲水性多孔膜，例如0.22微米的膜，并且支撑层是格栅或烧结的多孔材料。分配器和支撑件保持在一起以在膜周围形成一个信封。在这样做时优选使用铰链，夹子和其它这样的装置。

10. 发明申请

US20110256025A1 IMMUNOASSAY PRODUCT AND PROCESS 有权
标题翻译：免疫产品和过程
公开(公告)号：US20110256025A1
公开(公告)日：2011-10-20
申请号：US13078290
申请日：2011-04-01
申请人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
发明人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
IPC分类号： G01N33/53
CPC分类号： B01L3/5025 , B01L3/50255 , B01L3/50853 , B01L2200/0642 , B01L2300/043 , B01L2300/069 , B01L2300/0829 , B01L2400/0487
摘要： The invention is directed to an apparatus useful in conducting detection of compounds on blotting membranes. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and optionally a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane and the support layer is a grid or sintered porous material. The distributor and support are held together to form an envelope around the membrane(s). The use of a hinge, clips and other such devices is preferred in doing so.
摘要翻译：本发明涉及一种用于在印迹膜上进行化合物检测的装置。该装置包括多个层，包括印迹膜下面的多孔支撑层，印迹膜上方的流量分布器，以及任选的流量分配器上的孔，以将液体包含到期望的区域并允许降低这种液体的起始体积。优选地，流动分配器是非结合或低结合的亲水性多孔膜，例如0.22微米的膜，并且支撑层是格栅或烧结的多孔材料。分配器和支撑件保持在一起以在膜周围形成一个信封。在这样做时优选使用铰链，夹子和其它这样的装置。

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