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1. 发明授权

US08652421B2 Immunoassay product and process 有权
公开(公告)号：US08652421B2
公开(公告)日：2014-02-18
申请号：US11582599
申请日：2006-10-18
申请人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
发明人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
IPC分类号： G01N33/50 , C12M1/40
CPC分类号： B01L3/5025 , B01L3/50255 , B01L3/50853 , B01L2200/0642 , B01L2300/043 , B01L2300/069 , B01L2300/0829 , B01L2400/0487
摘要： The invention is directed to an apparatus useful in conducting detection of compounds on blotting membranes. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and optionally a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane and the support layer is a grid or sintered porous material. The distributor and support are held together to form an envelope around the membrane(s). The use of a hinge, clips and other such devices is preferred in doing so.

2. 发明申请

US20070243628A1 Immunoassay product and process 有权
标题翻译：免疫测定产品和方法
公开(公告)号：US20070243628A1
公开(公告)日：2007-10-18
申请号：US11582727
申请日：2006-10-18
申请人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
发明人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
IPC分类号： G01N33/53 , B01J19/00
CPC分类号： G01N33/5302 , B01L3/50255 , B01L2400/0487 , G01N33/54366 , G01N33/54393 , G01N33/561 , Y10T436/2575
摘要： A rapid, efficient and convenient method to detect one or more biological entities on a blotting membrane is provided. The detection can relate to the position, nature or amount of the biological substance on one or more membranes. The invention method involves a pressure assisted regiment (such as vacuum or positive gas pressure) for the supply and removal of reagents and permits washing of the contaminants from substances embedded in the membrane to be detected using very low volumes of liquid. This method enables completion of the blocking, washing and antibody binding steps in about 30 minutes without comprising blot quality. In another aspect, the invention is directed to an apparatus useful in conducting the method of the invention. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane.
摘要翻译：提供了一种快速，有效和方便地检测印迹膜上的一个或多个生物实体的方法。检测可以涉及一个或多个膜上的生物物质的位置，性质或数量。本发明方法包括用于供应和去除试剂的压力辅助团（例如真空或正气压），并且允许使用非常低体积的液体从嵌入在待检测膜中的物质洗涤污染物。该方法能够在约30分钟内完成阻断，洗涤和抗体结合步骤，而不包括印迹质量。另一方面，本发明涉及可用于实施本发明方法的装置。该装置由多个层组成，包括印迹膜下方的多孔支撑层，印迹膜上方的流动分布器以及流量分配器上的孔，以将液体容纳到所需区域并允许较低的开始这种液体的体积。优选地，流动分配器是非结合或低结合的亲水性多孔膜，例如0.22微米的膜。

3. 发明申请

US20070098601A1 Immunoassay product and process 有权
标题翻译：免疫测定产品和方法
公开(公告)号：US20070098601A1
公开(公告)日：2007-05-03
申请号：US11582599
申请日：2006-10-18
申请人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
发明人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
IPC分类号： B01L9/00
CPC分类号： B01L3/5025 , B01L3/50255 , B01L3/50853 , B01L2200/0642 , B01L2300/043 , B01L2300/069 , B01L2300/0829 , B01L2400/0487
摘要： The invention is directed to an apparatus useful in conducting detection of compounds on blotting membranes. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and optionally a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane and the support layer is a grid or sintered porous material. The distributor and support are held together to form an envelope around the membrane(s). The use of a hinge, clips and other such devices is preferred in doing so.
摘要翻译：本发明涉及一种用于在印迹膜上进行化合物检测的装置。该装置包括多个层，包括印迹膜下面的多孔支撑层，印迹膜上方的流量分布器，以及任选的流量分配器上的孔，以将液体包含到期望的区域并允许降低这种液体的起始体积。优选地，流动分配器是非结合或低结合的亲水性多孔膜，例如0.22微米的膜，并且支撑层是格栅或烧结的多孔材料。分配器和支撑件保持在一起以在膜周围形成一个信封。在这样做时优选使用铰链，夹子和其它这样的装置。

4. 发明授权

US08652422B2 Immunoassay product and process 有权
标题翻译：免疫测定产品和方法
公开(公告)号：US08652422B2
公开(公告)日：2014-02-18
申请号：US13078290
申请日：2011-04-01
申请人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
发明人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
IPC分类号： G01N33/50 , C12M1/40
CPC分类号： B01L3/5025 , B01L3/50255 , B01L3/50853 , B01L2200/0642 , B01L2300/043 , B01L2300/069 , B01L2300/0829 , B01L2400/0487
摘要： The invention is directed to an apparatus useful in conducting detection of compounds on blotting membranes. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and optionally a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane and the support layer is a grid or sintered porous material. The distributor and support are held together to form an envelope around the membrane(s). The use of a hinge, clips and other such devices is preferred in doing so.
摘要翻译：本发明涉及一种用于在印迹膜上进行化合物检测的装置。该装置包括多个层，包括印迹膜下面的多孔支撑层，印迹膜上方的流量分布器，以及任选的流量分配器上的孔，以将液体包含到期望的区域并允许降低这种液体的起始体积。优选地，流动分配器是非结合或低结合的亲水性多孔膜，例如0.22微米的膜，并且支撑层是格栅或烧结的多孔材料。分配器和支撑件保持在一起以在膜周围形成一个信封。在这样做时优选使用铰链，夹子和其它这样的装置。

5. 发明申请

US20160137961A2 Layered Flask Cell Culture System 有权
标题翻译：分层烧瓶细胞培养系统
公开(公告)号：US20160137961A2
公开(公告)日：2016-05-19
申请号：US12460265
申请日：2009-07-16
申请人： Phillip Clark , Kurt Greenizen , Christopher A. Scott , Marc Emerick
发明人： Phillip Clark , Kurt Greenizen , Christopher A. Scott , Marc Emerick
IPC分类号： C12M1/24 , C12M3/00 , C12M1/12
CPC分类号： C12M23/08 , C12M21/08 , C12M23/04 , C12M23/22 , C12M23/34 , C12M23/38 , C12M23/58 , C12M25/06 , C12M37/02
摘要： The present invention is a cell cultivating vessel or device, such as a single or multitier flask, including a cover having a top plate, a side wall and a resealable port; an intermediate tray for receiving cells and cell culture media, having a bottom plate, a side wall, and a gap region formed between an interior upwardly angled lip located on an interior portion of the intermediate tray bottom plate and an adjacent outwardly angled side wall portion of the intermediate tray bottom plate, wherein the lip has a outwardly swooping curvilinear edge feature; and a base tray for receiving the cells and cell culture media, including a bottom plate and a side wall. The intermediate tray is positioned between the cover and the base tray, such that the gap region of the intermediate tray bottom plate is in alignment with the port located on the cover, resulting in the port, the intermediate tray and the base tray in fluid communication with one another which provides direct access, such as by a user to remove and/or add cells, cell media, and nutrients located on each of the intermediate and/or the base trays. Alternatively, the cell cultivating flask includes a plurality of intermediate trays stacked on top of one another and the gap regions of each intermediate tray are in alignment with each other and with the port on the cover.
摘要翻译：本发明是一种细胞培养容器或装置，例如单个或多个烧瓶，包括具有顶板，侧壁和可再密封端口的盖; 用于接收细胞和细胞培养基的中间托盘，具有底板，侧壁和形成在位于中间托盘底板的内部部分上的内部向上倾斜的唇缘和相邻的向外成角度的侧壁部分之间的间隙区域的中间托盘底板，其中所述唇部具有向外的转动曲线边缘特征; 以及用于接纳细胞和细胞培养基的底盘，包括底板和侧壁。中间托盘定位在盖和基托之间，使得中间托盘底板的间隙区域与位于盖上的端口对准，导致端口，中间托盘和底盘处于流体连通彼此之间提供直接访问，诸如由用户去除和/或添加位于中间和/或底盘中的每一个上的细胞，细胞培养基和营养物质。或者，细胞培养瓶包括堆叠在彼此顶部的多个中间托盘，并且每个中间托盘的间隙区域彼此对准并且与盖上的端口对齐。

6. 发明授权

US09304070B2 All-in-one sample preparation device and method 有权
公开(公告)号：US09304070B2
公开(公告)日：2016-04-05
申请号：US13534570
申请日：2012-06-27
申请人： Christopher A. Scott , Timothy K. Nadler , Kurt Greenizen , Louis Bonhomme , Sara D. Gutierrez , Masaharu Mabuchi , David Briggs , Phillip Clark , Ralph T. Scaduto
发明人： Christopher A. Scott , Timothy K. Nadler , Kurt Greenizen , Louis Bonhomme , Sara D. Gutierrez , Masaharu Mabuchi , David Briggs , Phillip Clark , Ralph T. Scaduto
IPC分类号： B01L3/00 , B01D63/08 , B01D25/22 , B01D21/26 , G01N1/34 , G01N1/40 , B01D61/18 , B01D63/06 , B01D63/16 , C07K1/22 , C07K1/16
CPC分类号： G01N1/34 , B01D61/18 , B01D63/06 , B01D63/16 , B01D2315/02 , B01L3/5021 , B01L3/5085 , B01L2300/0618 , B01L2300/0681 , C07K1/16 , C07K1/22 , G01N1/405 , G01N1/4077 , G01N2001/4088 , Y10T436/25375
摘要： Sample preparation device that allows for a complete bind, wash, elute, buffer-exchange and concentration process to be carried out without sample transfer between multiple devices. The device includes a reservoir, a column for holding chromatography media, a holder region for holding a filtration device, and an outlet. The filtration device plugs into the holder region of the centrifugal device, and the assembly can be placed in an optional holder. The assembly, with or without the optional holder, can be placed in a conventional centrifuge tube for centrifugation. The entire bind, wash, elute, buffer exchange and concentration steps can be carried out with the apparatus without any pipette transfers (and the associated sample losses. The sample preparation device also can be used for binding and washing steps, in which case the filtration device is not needed, and for buffer exchange and concentration steps, in which case the media is not needed.

7. 发明申请

US20110038757A1 IMMUNOASSAY PRODUCT AND PROCESS 有权
标题翻译：免疫产品和过程
公开(公告)号：US20110038757A1
公开(公告)日：2011-02-17
申请号：US12911407
申请日：2010-10-25
申请人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
发明人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
IPC分类号： G01N33/48
CPC分类号： B01L3/5025 , B01L3/50255 , B01L3/50853 , B01L2200/0642 , B01L2300/043 , B01L2300/069 , B01L2300/0829 , B01L2400/0487
摘要： The invention is directed to an apparatus useful in conducting detection of compounds on blotting membranes. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and optionally a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane and the support layer is a grid or sintered porous material. The distributor and support are held together to form an envelope around the membrane(s). The use of a hinge, clips, and other such devices is preferred in doing so.
摘要翻译：本发明涉及一种用于在印迹膜上进行化合物检测的装置。该装置包括多个层，包括印迹膜下面的多孔支撑层，印迹膜上方的流量分布器，以及任选的流量分配器上的孔，以将液体包含到期望的区域并允许降低这种液体的起始体积。优选地，流动分配器是非结合或低结合的亲水性多孔膜，例如0.22微米的膜，并且支撑层是格栅或烧结的多孔材料。分配器和支撑件保持在一起以在膜周围形成一个信封。在这样做时优选使用铰链，夹子和其它这样的装置。

8. 发明授权

US09783769B2 Layered flask cell culture system 有权
公开(公告)号：US09783769B2
公开(公告)日：2017-10-10
申请号：US12460265
申请日：2009-07-16
申请人： Phillip Clark , Kurt Greenizen , Christopher A. Scott , Marc Emerick
发明人： Phillip Clark , Kurt Greenizen , Christopher A. Scott , Marc Emerick
IPC分类号： C12M1/24 , C12M1/00 , C12M1/12 , C12M3/00
CPC分类号： C12M23/08 , C12M21/08 , C12M23/04 , C12M23/22 , C12M23/34 , C12M23/38 , C12M23/58 , C12M25/06 , C12M37/02
摘要： The present invention is a cell cultivating vessel or device, such as a single or multitier flask, including a cover having a top plate, a side wall and a resealable port; an intermediate tray for receiving cells and cell culture media, having a bottom plate, a side wall, and a gap region formed between an interior upwardly angled lip located on an interior portion of the intermediate tray bottom plate and an adjacent outwardly angled side wall portion of the intermediate tray bottom plate, wherein the lip has a outwardly swooping curvilinear edge feature; and a base tray for receiving the cells and cell culture media, including a bottom plate and a side wall. The intermediate tray is positioned between the cover and the base tray, such that the gap region of the intermediate tray bottom plate is in alignment with the port located on the cover, resulting in the port, the intermediate tray and the base tray in fluid communication with one another which provides direct access, such as by a user to remove and/or add cells, cell media, and nutrients located on each of the intermediate and/or the base trays. Alternatively, the cell cultivating flask includes a plurality of intermediate trays stacked on top of one another and the gap regions of each intermediate tray are in alignment with each other and with the port on the cover.

9. 发明授权

US08557600B2 Immunoassay product and process 有权
标题翻译：免疫测定产品和方法
公开(公告)号：US08557600B2
公开(公告)日：2013-10-15
申请号：US11582727
申请日：2006-10-18
申请人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
发明人： Masaharu Mabuchi , Hiroko Kimura , Marc Emerick , Phillip Clark , Kurt Greenizen
IPC分类号： G01N1/10
CPC分类号： G01N33/5302 , B01L3/50255 , B01L2400/0487 , G01N33/54366 , G01N33/54393 , G01N33/561 , Y10T436/2575
摘要： A rapid, efficient and convenient method to detect one or more biological entities on a blotting membrane is provided. The detection can relate to the position, nature or amount of the biological substance on one or more membranes. The invention method involves a pressure assisted regiment (such as vacuum or positive gas pressure) for the supply and removal of reagents and permits washing of the contaminants from substances embedded in the membrane to be detected using very low volumes of liquid. This method enables completion of the blocking, washing and antibody binding steps in about 30 minutes without comprising blot quality. In another aspect, the invention is directed to an apparatus useful in conducting the method of the invention. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane.
摘要翻译：提供了一种快速，有效和方便地检测印迹膜上的一个或多个生物实体的方法。检测可以涉及一个或多个膜上的生物物质的位置，性质或数量。本发明方法包括用于供应和去除试剂的压力辅助团（例如真空或正气压），并且允许使用非常低体积的液体从嵌入在待检测膜中的物质洗涤污染物。该方法能够在约30分钟内完成封闭，洗涤和抗体结合步骤，而不包括印迹质量。另一方面，本发明涉及可用于实施本发明方法的装置。该装置由多个层组成，包括印迹膜下方的多孔支撑层，印迹膜上方的流动分布器以及流量分配器上的孔，以将液体容纳到所需区域并允许较低的开始这种液体的体积。优选地，流动分配器是非结合或低结合的亲水性多孔膜，例如0.22微米的膜。

10. 发明授权

US08460618B2 Immunoassay product and process 有权
标题翻译：免疫测定产品和方法
公开(公告)号：US08460618B2
公开(公告)日：2013-06-11
申请号：US12911407
申请日：2010-10-25
申请人： Masaharu Mabuchi , Hiroko Ykohama , Marc Emerick , Phillip Clark , Kurt Greenizen
发明人： Masaharu Mabuchi , Hiroko Ykohama , Marc Emerick , Phillip Clark , Kurt Greenizen
IPC分类号： G01N33/50 , C12M1/40
CPC分类号： B01L3/5025 , B01L3/50255 , B01L3/50853 , B01L2200/0642 , B01L2300/043 , B01L2300/069 , B01L2300/0829 , B01L2400/0487
摘要： The invention is directed to an apparatus useful in conducting detection of compounds on blotting membranes. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and optionally a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane and the support layer is a grid or sintered porous material. The distributor and support are held together to form an envelope around the membrane(s). The use of a hinge, clips and other such devices is preferred in doing so.
摘要翻译：本发明涉及一种用于在印迹膜上进行化合物检测的装置。该装置包括多个层，包括印迹膜下面的多孔支撑层，印迹膜上方的流量分布器，以及任选的流量分配器上的孔，以将液体包含到期望的区域并允许降低这种液体的起始体积。优选地，流动分配器是非结合或低结合的亲水性多孔膜，例如0.22微米的膜，并且支撑层是格栅或烧结的多孔材料。分配器和支撑件保持在一起以在膜周围形成一个信封。在这样做时优选使用铰链，夹子和其它这样的装置。

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