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1. 发明授权

US07879594B2 Method and device for detecting biomolecules 失效
标题翻译：用于检测生物分子的方法和装置
公开(公告)号：US07879594B2
公开(公告)日：2011-02-01
申请号：US10548869
申请日：2004-03-15
申请人： Bernard Mandrand , Agnes Dupont-Filliard
发明人： Bernard Mandrand , Agnes Dupont-Filliard
IPC分类号： C12M1/00
CPC分类号： B82Y5/00 , C12Q1/6825 , C12Q1/6837 , C12Q2563/125 , C12Q2565/113 , C12Q2565/607 , G01N33/5438 , Y10S977/70 , Y10S977/702 , Y10S977/704 , Y10S977/705 , Y10S977/72
摘要： The present invention relates to a method for detecting a target biomolecule in a sample comprising a plurality of biomolecules, whereby the target biomolecule is provided with a tag, said tag comprising a catalytic active moiety which catalyses a reaction yielding an insoluble reaction product which precipitates on flexible electrically conductive nanoelectrodes. The precipitation onto said nanoelectrodes causes a change in their electroconductivity which is accessible to electroanalytical methods. The invention relates further to a biochip comprising a solid support with nanoelectrodes attached thereto and probe molecules bound to all or to a plurality of said nanoelectrodes which may be the same or different, a segment of said probe molecules being able to interact specifically with a segment of the target biomolecules.
摘要翻译：本发明涉及用于检测包含多个生物分子的样品中的目标生物分子的方法，由此所述靶生物分子被提供有标签，所述标签包含催化活性部分，其催化产生不溶性反应产物的反应，所述反应产物在柔性导电纳米电极。所述纳米电极上的沉淀导致其电导率的变化，其可用于电分析方法。本发明进一步涉及一种生物芯片，其包括固体支持体，其上附着有纳米电极，以及探针分子结合到全部或多个所述纳米电极上，所述纳米电极可以相同或不同，所述探针分子的一段能够与片段的目标生物分子。

2. 发明申请

US20070037153A1 Method and device for detecting biomolecules 失效
标题翻译：用于检测生物分子的方法和装置
公开(公告)号：US20070037153A1
公开(公告)日：2007-02-15
申请号：US10548869
申请日：2004-03-15
申请人： Bernard Mandrand , Agnes Dupont-Filliard
发明人： Bernard Mandrand , Agnes Dupont-Filliard
IPC分类号： C12Q1/68 , G01N33/53
CPC分类号： B82Y5/00 , C12Q1/6825 , C12Q1/6837 , C12Q2563/125 , C12Q2565/113 , C12Q2565/607 , G01N33/5438 , Y10S977/70 , Y10S977/702 , Y10S977/704 , Y10S977/705 , Y10S977/72
摘要： The present invention relates to a method for detecting a target biomolecule in a sample comprising a plurality of biomolecules, whereby the target biomolecule is provided with a tag, said tag comprising a catalytic active moiety which catalyses a reaction yielding an insoluble reaction product which precipitates on flexible electrically conductive nanoelectrodes. The precipitation onto said nanoelectrodes causes a change in their electroconductivity which is accessible to electroanalytical methods. The invention relates further to a biochip comprising a solid support with nanoelectrodes attached thereto and probe molecules bound to all or to a plurality of said nanoelectrodes which may be the same or different, a segment of said probe molecules being able to interact specifically with a segment of the target biomolecules.
摘要翻译：本发明涉及用于检测包含多个生物分子的样品中的靶生物分子的方法，由此所述靶生物分子被提供有标签，所述标签包含催化活性部分，其催化产生不溶性反应产物的反应，所述反应产物在柔性导电纳米电极。所述纳米电极上的沉淀导致其电导率的变化，其可用于电分析方法。本发明进一步涉及一种生物芯片，其包括固体支持体，其上附着有纳米电极，并且探针分子结合到所有或多个所述纳米电极上，所述纳米电极可以相同或不同，所述探针分子的一段能够与片段的目标生物分子。

3. 发明授权

US07771927B1 Multiple sclerosis-associated retroviral (MSRV) nucleic acids corresponding to the Env region 失效
标题翻译：对应于Env区域的多发性硬化相关逆转录病毒（MSRV）核酸
公开(公告)号：US07771927B1
公开(公告)日：2010-08-10
申请号：US09319156
申请日：1998-07-07
申请人： Glaucia Paranhos-Baccala , Florence Komurian-Pradel , Frederic Bedin , Mireille Sodoyer , Catherine Ott , Francois Mallet , Herve Perron , Bernard Mandrand
发明人： Glaucia Paranhos-Baccala , Florence Komurian-Pradel , Frederic Bedin , Mireille Sodoyer , Catherine Ott , Francois Mallet , Herve Perron , Bernard Mandrand
IPC分类号： C12Q1/70 , C07H21/04 , A61K39/21
CPC分类号： C07K14/4713 , C07K14/005 , C12N2740/10022
摘要： The invention concerns a nucleic material, in isolated or purified state, and a nucleotide fragment comprising a nucleotide sequence selected from the group consisting in (i) the sequences SEQ ID NO: 112, SEQ ID NO:114, SEQ ID NO: 117, SEQ ID NO: 120, SEQ ID NO. 124, SEQ ID NO: 130, SEQ ID NO: 141 and SEQ ID NO: 142, (ii) the complementary sequences of sequences (i); and (iii) the sequences equivalent to sequences (ii) and (iii), in particular the sequence having for every series of 100 contiguous monomers, at least 50%, preferably 70% homology with sequences (i) and (ii) respectively. The invention also concerns their uses for detecting a retrovirus associated with multiple sclerosis and/or rheumatoid arthritis.
摘要翻译：本发明涉及分离或纯化状态的核酸，以及包含选自（i）序列SEQ ID NO：112，SEQ ID NO：114，SEQ ID NO：117， SEQ ID NO：120，SEQ ID NO： 124，SEQ ID NO：130，SEQ ID NO：141和SEQ ID NO：142，（ii）序列（i）的互补序列; 和（iii）与序列（ii）和（iii）相当的序列，特别是具有每100个连续单体的序列的序列，分别与序列（i）和（ii）具有至少50％，优选70％的同源性。本发明还涉及其用于检测与多发性硬化和/或类风湿性关节炎相关的逆转录病毒的用途。

4. 发明授权

US07674888B2 Viral material and nucleotide fragments associated with multiple sclerosis, for diagnostic, prophylactic and therapeutic purposes 有权
标题翻译：与多发性硬化相关的病毒材料和核苷酸片段，用于诊断，预防和治疗目的
公开(公告)号：US07674888B2
公开(公告)日：2010-03-09
申请号：US11581030
申请日：2006-10-16
申请人： Herve Perron , Frederic Beseme , Frederic Bedin , Glaucia Paranhos-Baccala , Florence Komurian-Pradel , Colette Jolivet-Reynaud , Bernard Mandrand , Jeremy Alexander Garson , Philip William Tuke
发明人： Herve Perron , Frederic Beseme , Frederic Bedin , Glaucia Paranhos-Baccala , Florence Komurian-Pradel , Colette Jolivet-Reynaud , Bernard Mandrand , Jeremy Alexander Garson , Philip William Tuke
IPC分类号： C07H21/02
CPC分类号： C07K14/005 , A61K38/00 , C12N7/00 , C12N9/1241 , C12N2740/10022 , C12N2740/10051 , C12Q1/702
摘要： The invention provides viral material and nucleotide fragments associated with multiple sclerosis and/or rheumatoid arthritis for use in method of diagnosis, prophylaxis, and therapy.
摘要翻译：本发明提供了用于诊断，预防和治疗方法的与多发性硬化和/或类风湿性关节炎有关的病毒物质和核苷酸片段。

5. 发明授权

US07060804B2 Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses 有权
公开(公告)号：US07060804B2
公开(公告)日：2006-06-13
申请号：US10182126
申请日：2001-01-22
申请人： Abdelhamid Elaissari , Bernard Mandrand , Thierry Delair , Doran Spencer , Ahmed Arkis
发明人： Abdelhamid Elaissari , Bernard Mandrand , Thierry Delair , Doran Spencer , Ahmed Arkis
IPC分类号： A23J1/00 , C07K1/00 , C07K14/00 , C07K16/00 , C07K17/00
CPC分类号： C12N15/1013 , C07K1/22 , G01N33/5434
摘要： The invention concerns a method for isolating proteins and/or protein and nucleic acid associations in a sample, comprising steps which consist in: contacting said sample and magnetic colloidal particles comprising a core and a coat wherein: the core is magnetic and is coated with at least a polymer comprising functional groups X selected among amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups or mixtures thereof, whereof at least one fraction has reacted with other functional groups of the coat, and the coat consists of a polymer bearing functional groups Z and Z′, capable of ionisation, identical or different, selected among amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulphide, α-halogenocarbonyl, sulphonic acid, maleimide, isocyanate and isothiocyanate groups to form a mixture; incubating said mixture in predetermined conditions; and separating from the mixture the protein and/or protein and nucleic acid associations complexed on the colloidal particles by applying a magnetic field. The invention also concerns a complex consisting of colloidal particles and proteins, a reagent comprising such a complex or colloidal particles.

6. 发明授权

US06270953B1 Method for detecting and/or quantifying a gliotoxic factor 失效
标题翻译：用于检测和/或定量胶质毒性因子的方法
公开(公告)号：US06270953B1
公开(公告)日：2001-08-07
申请号：US09202118
申请日：1999-03-29
申请人： Carine Malcus-Vocanson , Herve Perron , Bernard Mandrand
发明人： Carine Malcus-Vocanson , Herve Perron , Bernard Mandrand
IPC分类号： C12Q100
CPC分类号： G01N33/564 , G01N33/5014 , G01N33/6896
摘要： The invention proposes a method for detecting and/or quantifying, in a biological sample, a cytotoxic factor, in particular a gliotoxic factor, with respect to adherent target cells, in particular macroglial cells, the toxicity of which causes the death by apoptosis of said cells. The method comprises providing an initial fraction of the sample, optionally enriched with the toxic factor by previous treatment, incubating the initial toxic factor with a reference culture medium comprising adherent target cells, and detecting and/or quantifying in the adherent target cells killed by apoptosis, by flow cytometry, at least one direct or indirect characteristic associated with the apoptotic adherent cells of the whole or part of the incubated medium, which, if it is present and/or is quantified, qualifies the sample as positive, i.e. as containing said toxic factor. The initial biological sample is preferably a urine specimen.
摘要翻译：本发明提出了一种用于在生物样品中检测和/或定量细胞毒性因子，特别是胶质毒性因子的方法，所述细胞毒性因子涉及粘附的靶细胞，特别是大胶质细胞，其毒性导致所述细胞。该方法包括提供样品的初始级分，任选地通过先前的处理富集毒性因子，将初始毒性因子与包含贴壁靶细胞的参照培养基孵育，并在凋亡死亡的贴壁靶细胞中检测和/或定量通过流式细胞术，与整个或部分培养的培养基的凋亡贴壁细胞相关联的至少一个直接或间接特征，如果存在和/或定量，则将样品鉴定为阳性，即含有所述毒性因素。初始生物样品优选为尿样。

7. 发明授权

US5876954A Cytotoxic factor as is associated with multiple sclerosis, its detection and its quantification 失效
标题翻译：与多发性硬化相关的细胞毒性因子，其检测及其定量
公开(公告)号：US5876954A
公开(公告)日：1999-03-02
申请号：US389164
申请日：1995-02-15
申请人： Herve Perron , Tomas Dobransky , Fran.cedilla.ois Rieger , Bernard Mandrand
发明人： Herve Perron , Tomas Dobransky , Fran.cedilla.ois Rieger , Bernard Mandrand
IPC分类号： G01N33/53 , A61K38/00 , C07K1/18 , C07K14/47 , C12Q1/02 , G01N33/50 , G01N33/566 , C12Q1/37 , C12Q1/00 , C12Q1/34
CPC分类号： C07K14/4713 , G01N33/5091 , A61K38/00 , Y10S424/16 , Y10S435/964 , Y10S436/827 , Y10S436/828
摘要： Gliotoxic factor in the isolated or purified state, characterized in that it possesses toxic activity with respect to human or animal astrocytic cells, having the effect of a cytomorphological disorganization of their network of intermediate filaments and/or a degradation of the proteins of said intermediate filaments and/or cell death, in particular by apoptosis.
摘要翻译：分离或纯化状态下的胶质毒性因子，其特征在于其对人或动物星形细胞细胞具有毒性活性，具有其中间丝的网络的细胞形态学紊乱和/或所述中间丝的蛋白质降解的作用和/或细胞死亡，特别是凋亡。

8. 发明授权

US5824517A Method for amplifying nucleic acid sequences by strand displacement using DNA/RNA chimeric primers 失效
标题翻译：使用DNA / RNA嵌合引物通过链置换扩增核酸序列的方法
公开(公告)号：US5824517A
公开(公告)日：1998-10-20
申请号：US817035
申请日：1997-05-16
申请人： Philippe Cleuziat , Bernard Mandrand
发明人： Philippe Cleuziat , Bernard Mandrand
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6844
摘要： A target nucleic acid may be amplified in the presence of an enzymatic system including DNA polymerase, strand translocation and RNAse H activities by using a chimeric primer that includes, in the 5' to 3' direction, an RNA-type segment capable of hybridizing with a 3'-terminal segment of the target and a DNA-type segment capable of hybridizing with a segment adjacent to the 3'-terminal segment of the target and a DNA- or RNA-type primer capable of hybridizing with the 3'-terminal segment of the target. A cyclic amplification that may be implemented isothermally on the basis of either a DNA or an RNA target is achieved even when the terminals are not defined.
摘要翻译： PCT No.PCT / FR96 / 01166 Sec。 371日期：1997年5月16日 102（e）日期1997年5月16日PCT提交1996年7月24日PCT公布。出版物WO97 / 04126 日期1997年2月6日目标核酸可以在包括DNA聚合酶，链转运和RNA酶H活性的酶系统存在下扩增，使用包含在5'至3'方向的RNA型的嵌合引物能够与靶标的3'末端区段杂交的区段和能够与靶标的3'-末端区段相邻的区段杂交的DNA型区段以及能够与靶标杂交的DNA或RNA型引物目标的3'-末端区段。即使没有限定端子，也可以基于DNA或RNA靶标等温地进行循环扩增。

9. 发明授权

US5723344A Device for the capture of target molecules, and capturing process using the device 失效
标题翻译：用于捕获目标分子的装置，以及使用该装置的捕获过程
公开(公告)号：US5723344A
公开(公告)日：1998-03-03
申请号：US264996
申请日：1994-06-24
申请人： Claude Mabilat , Philippe Cros , Bernard Mandrand , Marie-Helene Charles , Marie-Noelle Erout , Christian Pichot
发明人： Claude Mabilat , Philippe Cros , Bernard Mandrand , Marie-Helene Charles , Marie-Noelle Erout , Christian Pichot
IPC分类号： G01N33/543 , C12Q1/68 , G01N33/544
CPC分类号： G01N33/54353 , G01N33/54366 , Y10S435/975 , Y10S436/807
摘要： Device for capturing a target molecule for the purpose of detecting it and/or assaying it, including a solid support on which is immobilized a ligand, the ligand being provided in the form of a conjugate resulting from the covalent coupling of a polymer with a plurality of molecules of the ligand. The polymer is an N-vinylpyrrolidone copolymer, and the conjugate is immobilized on the solid support by adsorption. When the ligand is capable of forming a complex with the target, the device is specific for a given target. When the device comprises, in addition, a bifunctional reagent capable of forming a complex, on the one hand, with the ligand and, on the other hand, with the target, the support on which the ligand is immobilized constitutes a universal capturing system.
摘要翻译：用于捕获靶分子以用于检测和/或测定靶分子的装置，包括其上固定有配体的固体支持体，所述配体以由聚合物与多个共价键共价偶联得到的缀合物形式提供的配体分子。聚合物是N-乙烯基吡咯烷酮共聚物，并且通过吸附将缀合物固定在固体载体上。当配体能够与目标物形成复合物时，该装置对于给定的靶标是特异性的。此外，当该装置另外包含能够与配体一起形成络合物的双功能试剂，另一方面，另外，与靶标配位的配体固定的载体构成通用捕获系统。

10. 发明申请

US20090314660A1 Novel Electropolymerisable Monomers, Soluble in an Aqueous Solution and Comprising a Metalloporphyrin 失效
标题翻译：新型可电聚合单体，可溶于水溶液并包含金属卟啉
公开(公告)号：US20090314660A1
公开(公告)日：2009-12-24
申请号：US12084476
申请日：2006-11-02
申请人： Frédéric Canonne , Hafsa Korri-Youssoufi , Jean-Pierre Mahy , Bernard Mandrand , Martine Perree-Fauvet
发明人： Frédéric Canonne , Hafsa Korri-Youssoufi , Jean-Pierre Mahy , Bernard Mandrand , Martine Perree-Fauvet
IPC分类号： G01N27/26 , H01B1/12 , C07D207/46 , C07H21/00 , C07K14/00 , C07K16/00 , C25B3/00
CPC分类号： C08G61/124 , C07D487/22 , G01N27/48
摘要： The invention relates to novel electropolymerisable monomers which are to be polymerised in an aqueous solution and comprise: an electropolymerisable pattern selected from acetylene, pyrrols, thiophenes, indols, anilines, azines, p-phenylene vinylenes, p-phenylenes, pyrenes, furanes, selenophenes, pyrridazines, carbazoles, acrylates, methacrylates and the derivatives thereof, and a metalloporphyrine which is substituted by at least two ionised or ionisable entities in an aqueous solution. The invention also relates to a method for the polymerisation of such monomers, to the electroactive probe that can be obtained by the polymerisation of such monomers, and to a method for detecting a target ligand in a biological sample using one such electroactive probe.
摘要翻译：本发明涉及要在水溶液中聚合的新型可电聚合单体，包括：可电聚合图案，其选自乙炔，吡咯，噻吩，吲哚，苯胺，吖嗪，对亚苯基乙烯，对亚苯基，芘，呋喃，硒吩吡咯嗪，咔唑，丙烯酸酯，甲基丙烯酸酯及其衍生物，以及在水溶液中由至少两个电离或电离实体取代的金属卟啉。本发明还涉及这种单体的聚合方法，可以通过这些单体的聚合获得的电活性探针，以及使用一种这样的电活性探针检测生物样品中的靶配体的方法。

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