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1. 发明申请

US20060263822A1 Detection of rpoB sequences of mycobacterium tuberculosis 有权
标题翻译：检测结核分枝杆菌rpoB序列
公开(公告)号：US20060263822A1
公开(公告)日：2006-11-23
申请号：US11494963
申请日：2006-07-28
申请人： Markus Jucker , Steven Brentano , Francisco Delgado , Philippe Cleuziat
发明人： Markus Jucker , Steven Brentano , Francisco Delgado , Philippe Cleuziat
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/689 , C12Q2600/156
摘要： A method of detecting rpoB sequences of Mycobacterium tuberculosis present in a biological sample that includes steps of amplifying the M. tuberculosis rpoB sequence in vitro in a nucleic acid amplification mixture that includes specific disclosed primer sequences, and detecting the amplified sequences by using probes that provide information by their specific hybridization to portions of the amplified nucleic acid is disclosed. Compositions for amplifying and detecting in vitro the rpoB sequences of M. tuberculosis in a sample are disclosed.
摘要翻译：一种检测存在于生物样品中的结核分枝杆菌的rpoB序列的方法，其包括在包括特异性公开的引物序列的核酸扩增混合物中体外扩增结核分枝杆菌rpoB序列的步骤，并通过使用提供的探针检测扩增的序列公开了通过其与部分扩增核酸的特异性杂交的信息。公开了用于在体外扩增和检测样品中结核分枝杆菌的rpoB序列的组合物。

2. 发明授权

US07879581B2 Nucleic acid amplification and detection of mycobacterium species 有权
标题翻译：核酸扩增和检测分枝杆菌种
公开(公告)号：US07879581B2
公开(公告)日：2011-02-01
申请号：US11938684
申请日：2007-11-12
申请人： Steven T. Brentano , Markus T. Jucker , Francisco D. Delgado , Philippe Cleuziat , Marc Rodrigue
发明人： Steven T. Brentano , Markus T. Jucker , Francisco D. Delgado , Philippe Cleuziat , Marc Rodrigue
IPC分类号： C12P19/32 , C07H21/04
CPC分类号： C12Q1/689 , C12Q2600/16
摘要： Oligonucleotides used to prime in vitro nucleic acid amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences for many species within the genus Mycobacterium are disclosed. Kits including such oligonucleotides are disclosed. Methods of detecting Mycobacterium species using the oligonucleotides in in vitro nucleic acid amplification are disclosed.
摘要翻译：公开了用于引发16S rRNA序列的体外核酸扩增的寡核苷酸或编码16S rRNA序列的DNA，用于分枝杆菌属中的许多物种。公开了包括这种寡核苷酸的试剂盒。公开了在体外核酸扩增中使用寡核苷酸检测分枝杆菌物种的方法。

3. 发明申请

US20090029360A1 Nucleic Acid Amplification and Detection of Mycobacterium Species 有权
标题翻译：核酸扩增和检测分枝杆菌种
公开(公告)号：US20090029360A1
公开(公告)日：2009-01-29
申请号：US11938684
申请日：2007-11-12
申请人： Steven T. Brentano , Markus T. Jucker , Francisco D. Delgado , Philippe Cleuziat , Marc Rodrigue
发明人： Steven T. Brentano , Markus T. Jucker , Francisco D. Delgado , Philippe Cleuziat , Marc Rodrigue
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q2600/16
摘要： Oligonucleotides used to prime in vitro nucleic acid amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences for many species within the genus Mycobacterium are disclosed. Kits including such oligonucleotides are disclosed. Methods of detecting Mycobacterium species using the oligonucleotides in in vitro nucleic acid amplification are disclosed.
摘要翻译：公开了用于引发16S rRNA序列的体外核酸扩增的寡核苷酸或编码16S rRNA序列的DNA，用于分枝杆菌属中的许多物种。公开了包括这种寡核苷酸的试剂盒。公开了在体外核酸扩增中使用寡核苷酸检测分枝杆菌物种的方法。

4. 发明授权

US6015531A Single-use analysis card comprising a liquid flow duct 失效
标题翻译：一次性分析卡，包括液体流动管道
公开(公告)号：US6015531A
公开(公告)日：2000-01-18
申请号：US983492
申请日：1998-03-05
申请人： Bruno Colin , Cecile Jaravel , Philippe Cleuziat
发明人： Bruno Colin , Cecile Jaravel , Philippe Cleuziat
IPC分类号： G01N33/543 , B01L3/00 , G01N35/02 , G01N21/00
CPC分类号： B01L3/502
摘要： The invention features an analysing device (1) comprising a body (2) in which are arranged or provided: an intake aperture (3) for a starting liquid sample, a liquid flow circuit (5) comprising at least one operating cell (6) for a processed liquid sample, obtained from all or part of the original sample, communicating with the said intake aperture (3), the said flow circuit defining, in at least two dimensions of the card, one determined geometric line, such that any alteration in the card orientation in a three-dimensional reference frame, causes the liquid to flow under gravity only, from one part of the said circuit to another,for instance from one side or another of the operating cell, (6) characterised in that, the flow circuit (5) is continuous, and looped on itself between the said aperture (3) and the said operating cell (6).
摘要翻译： PCT No.PCT / FR97 / 01020 Sec。 371日期：1998年3月5日 102（e）1998年3月5日PCT 1997年6月9日PCT公布。 WO97 / 46318 PCT公开号日期1997年12月11日本发明的特征在于一种分析装置（1），包括其中布置或提供的主体（2）;用于起始液体样品的进气孔（3），包括至少一个从所述原始样品的全部或部分获得的用于经处理的液体样品的操作室（6）与所述进气孔（3）连通，所述流动回路在卡的至少两个维度上限定一个确定的几何线，使得三维参考系中的卡方向的任何改变使得液体仅在重力作用下从所述电路的一部分流向另一部分，例如来自操作单元的一侧或另一侧（6 ），其特征在于，流动回路（5）是连续的，并且在所述孔（3）和所述操作单元（6）之间自身环绕。

5. 发明授权

US07294489B2 Nucleic acid amplification and detection of Mycobacterium species 有权
标题翻译：核酸扩增和检测分枝杆菌种
公开(公告)号：US07294489B2
公开(公告)日：2007-11-13
申请号：US10665708
申请日：2003-09-18
申请人： Steven T. Brentano , Markus T. Jucker , Francisco D. Delgado , Philippe Cleuziat , Marc Rodrigue
发明人： Steven T. Brentano , Markus T. Jucker , Francisco D. Delgado , Philippe Cleuziat , Marc Rodrigue
IPC分类号： C12P19/34 , C07H21/04
CPC分类号： C12Q1/689 , C12Q2600/16
摘要： Oligonucleotides used to prime in vitro nucleic acid amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences for many species within the genus Mycobacterium are disclosed. Kits including such oligonucleotides are disclosed. Methods of detecting Mycobacterium species using the oligonucleotides in in vitro nucleic acid amplification are disclosed.
摘要翻译：公开了用于引发16S rRNA序列的体外核酸扩增的寡核苷酸或编码16S rRNA序列的DNA，用于分枝杆菌属中的许多物种。公开了包括这种寡核苷酸的试剂盒。公开了在体外核酸扩增中使用寡核苷酸检测分枝杆菌物种的方法。

6. 发明申请

US20050100915A1 Nucleic acid amplification and detection of Mycobacterium species 有权
标题翻译：核酸扩增和检测分枝杆菌种
公开(公告)号：US20050100915A1
公开(公告)日：2005-05-12
申请号：US10665708
申请日：2003-09-18
申请人： Steven Brentano , Markus Jucker , Francisco Delgado , Philippe Cleuziat , Marc Rodrigue
发明人： Steven Brentano , Markus Jucker , Francisco Delgado , Philippe Cleuziat , Marc Rodrigue
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/689 , C12Q2600/16
摘要： Oligonucleotides used to prime in vitro nucleic acid amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences for many species within the genus Mycobacterium are disclosed. Kits including such oligonucleotides are disclosed. Methods of detecting Mycobacterium species using the oligonucleotides in in vitro nucleic acid amplification are disclosed.
摘要翻译：公开了用于引发16S rRNA序列的体外核酸扩增的寡核苷酸或编码16S rRNA序列的DNA，用于分枝杆菌属中的许多物种。公开了包括这种寡核苷酸的试剂盒。公开了在体外核酸扩增中使用寡核苷酸检测分枝杆菌物种的方法。

7. 发明授权

US06686195B1 Method and apparatus for ultrasonic lysis of biological cells 失效
标题翻译：生物细胞超声裂解的方法和装置
公开(公告)号：US06686195B1
公开(公告)日：2004-02-03
申请号：US09937992
申请日：2001-12-13
申请人： Bruno Colin , Philippe Cleuziat , Patrick Broyer , Claude Mabilat , Sandra Incardona
发明人： Bruno Colin , Philippe Cleuziat , Patrick Broyer , Claude Mabilat , Sandra Incardona
IPC分类号： C12M133
CPC分类号： B01J19/10 , B06B3/00 , C12M45/02
摘要： An apparatus including at least one sonotrode (2) designed to generate ultrasound of variable power in at least one biological sample (5) containing cells to be lysed, the sample (5) being contained in at least one receptacle (4 or 10) such that the sonotrode (2) is in direct contact with the receptacle(s) (4). Also disclosed is a method for using ultrasound to lyse a biological sample (5) contained in a receptacle (4), which includes placing the receptacle (4) in direct contact with the sonotrode (2), and activating the sonotrode (2) for long enough to lyse the cells in the sample (5) but preserve the DNA and/or RNA molecules released for subsequent operations, e.g. amplification. The invention is particularly applicable in the discipline of molecular biology.
摘要翻译：一种装置，包括至少一个超声波发生器（2），其被设计成在至少一个含有待裂解细胞的生物样品（5）中产生可变功率的超声波，所述样品（5）包含在至少一个容器（4或10）中，超声波发生器（2）与容器（4）直接接触。还公开了一种使用超声波来溶解包含在容器（4）中的生物样品（5）的方法，其包括将容器（4）放置在与超声焊极（2）直接接触的位置，并且激活超声焊极（2）以用于足以裂解样品（5）中的细胞，但保留释放用于后续操作的DNA和/或RNA分子，例如放大。本发明特别适用于分子生物学学科。

8. 发明授权

US06664081B2 Nucleic acid amplification and detection of mycobacterium species 有权
标题翻译：核酸扩增和检测分枝杆菌种
公开(公告)号：US06664081B2
公开(公告)日：2003-12-16
申请号：US09738274
申请日：2000-12-15
申请人： Steven T. Brentano , Markus T. Jucker , Francisco D. Delgado , Philippe Cleuziat , Marc Rodrigue
发明人： Steven T. Brentano , Markus T. Jucker , Francisco D. Delgado , Philippe Cleuziat , Marc Rodrigue
IPC分类号： C12P1934
CPC分类号： C12Q1/689 , C12Q2600/16
摘要： Methods of detecting Mycobacterium species using oligonucleotides to amplify in vitro 16S rRNA sequences or DNA encoding 16S rRNA sequences for many species in the genus Mycobacterium are disclosed. Amplification oligonucleotides for in vitro amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences from many Mycobacterium species are disclosed. Kits containing oligonucleotides useful for in vitro amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences from many Mycobacterium species are disclosed.
摘要翻译：公开了使用寡核苷酸来检测分枝杆菌物种的方法来扩增体外16S rRNA序列或编码16S rRNA序列的DNA，用于分枝杆菌属中的许多物种。公开了用于体外扩增来自许多分枝杆菌物种的16S rRNA序列或编码16S rRNA序列的DNA的扩增寡核苷酸。公开了含有可用于体外扩增16S rRNA序列的寡核苷酸或编码来自许多分枝杆菌物种的16S rRNA序列的DNA的试剂盒。

9. 发明授权

US5744308A Chimera oligonucleotide and its utilization for obtaining transcripts of a nucleic acid 失效
标题翻译：嵌合体寡核苷酸及其用于获得核酸转录本的用途
公开(公告)号：US5744308A
公开(公告)日：1998-04-28
申请号：US533912
申请日：1995-09-26
申请人： Fran.cedilla.oise Guillou-Bonnici , Philippe Cleuziat , Fran.cedilla.ois Mallet , Pierre Levasseur , William McAllister
发明人： Fran.cedilla.oise Guillou-Bonnici , Philippe Cleuziat , Fran.cedilla.ois Mallet , Pierre Levasseur , William McAllister
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6865
摘要： A chimera oligonucleotide is provided that can be used in a process for obtaining transcripts and/or amplification of a target sequence of a nucleic acid, having, at its 3' end, a downstream sequence. The oligonucleotide comprises successively, from 5' to 3', 1) a first oligonucleotide segment, of the DNA type, comprising a sense sequence of a promoter of an RNA polymerase, 2) a second oligonucleotide segment, of the DNA type, capable of hybridizing with the downstream sequence, and 3) a third oligonucleotide segment, of the RNA type, capable of hybridizing with a part of the target sequence contiguous to the downstream sequence, the third segment being blocked at 3'. A process using the chimera oligonucleotide and an enzyme system containing DNA polymerase activity, RNA polymerase activity, and a third activity, for example, an RNase H activity provides transcription products of the target. By adding a second chimera oligonucleotide capable of hybridizing with the complement of the target, cyclic amplification of the target and its complement are obtained.
摘要翻译：提供嵌合体寡核苷酸，其可用于获得在其3'末端具有下游序列的核酸的靶序列的转录物和/或扩增的方法。所述寡核苷酸依次包含5'至3'，1）DNA型的第一寡核苷酸片段，其包含RNA聚合酶的启动子的有义序列，2）DNA型的第二寡核苷酸片段，能够与下游序列杂交，以及3）能够与下游序列连续的靶序列的一部分杂交的RNA型的第三寡核苷酸片段，第3片段在3'处被阻断。使用嵌合寡核苷酸的方法和含有DNA聚合酶活性，RNA聚合酶活性和第三活性的酶系统，例如RNase H活性提供靶标的转录产物。通过添加能够与靶的补体杂交的第二嵌合寡核苷酸寡核苷酸寡核苷酸寡核苷酸，获得靶标及其互补序列的循环扩增。

10. 发明授权

US07723095B2 Method for cellular lysis of prokaryotes or eukaryotes or simultaneous cellular lysis of prokaryotes and eukaryotes 有权
标题翻译：细胞裂解原核生物或真核生物或同时细胞裂解原核生物和真核生物的方法
公开(公告)号：US07723095B2
公开(公告)日：2010-05-25
申请号：US10343109
申请日：2001-07-26
申请人： Philippe Cleuziat , Sandra Incardona , Corinne Jay
发明人： Philippe Cleuziat , Sandra Incardona , Corinne Jay
IPC分类号： C12N1/00
CPC分类号： C12N1/06 , C12M47/06
摘要： A method for lysing prokaryotic or eukaryotic cells, or for simultaneous lysis of both, which includes at least three of the following: a mass of active, small-diameter beads corresponding to 50% or less than a mass of active, larger-diameter beads, and/or a total mass of lysing beads (consisting of a single type of bead or a mixture of smaller and larger beads) corresponding to between 50 and 100% of the total mass of the processed biological sample, and/or a lysis time of from 10 to 20 minutes, and/or seven or less non-lysing glass beads to drive the movement of the lysing beads, and/or from five to fifteen non-lysing iron beads to drive the movement of the lysing beads, depending on whether sonication, mechanical vortex centrifugation or magnetic vortex centrifugation is used.
摘要翻译：用于裂解原核或真核细胞或同时裂解两者的方法，其包括以下至少三种：大量活性的小直径珠子，其对应于活性较大直径珠粒的质量的50％或更小，和/或相当于处理的生物样品总质量的50-100％的裂解珠（由单一类型的珠粒或较小和较大珠粒的混合物组成）的总质量，和/或裂解时间 10分钟至20分钟，和/或7个或更少的非裂解玻璃珠，以驱动裂解珠的移动，和/或5至15个非裂解铁珠以驱动溶胞珠的运动，这取决于是否使用超声处理，机械涡流离心或磁涡旋离心。

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