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1. 发明授权

US6057773A Unanchored sensor for fluid characteristics 失效
标题翻译：不带传感器的流体特性
公开(公告)号：US6057773A
公开(公告)日：2000-05-02
申请号：US094629
申请日：1998-06-15
申请人： Ashok K. Shukla , Mukta M Shukla
发明人： Ashok K. Shukla , Mukta M Shukla
IPC分类号： G01F23/00 , G01F23/28 , G01F23/36 , G01F23/64 , G01F23/68 , G01F23/70 , G01F23/76 , G01K13/02 , G01N27/07 , G01N27/28 , G01N33/18 , H01H29/20 , H01H35/18 , G08B21/00
CPC分类号： B01F13/0818 , B01F15/00207 , B01F15/00266 , G01F23/003 , G01F23/0038 , G01F23/282 , G01F23/36 , G01F23/64 , G01F23/68 , G01F23/70 , G01F23/76 , G01K13/02 , G01N33/1886 , H01H35/18 , G01N27/4167 , H01H29/20 , H01H35/186
摘要： A device and method for sensing fluid characteristics, including, temperature, pH and chemistry, comprises a switch affixed to or embedded in a container of a size, shape and density such that said container floats stable in said liquid sensors which measure and report other characteristics of the liquid, such as temperature, pH, viscosity, chemistry or biochemistry.
摘要翻译：用于检测流体特性（包括温度，pH和化学）的装置和方法包括固定到或嵌入容器中的尺寸，形状和密度的开关，使得所述容器在所述液体传感器中稳定地浮动，所述液体传感器测量并报告其它特性的液体，如温度，pH，粘度，化学或生物化学。

2. 发明申请

US20120045788A1 Device for sample preparation 审中-公开
标题翻译：样品制备装置
公开(公告)号：US20120045788A1
公开(公告)日：2012-02-23
申请号：US13199104
申请日：2011-08-19
申请人： Ashok K. Shukla , Mukta M. Shukla
发明人： Ashok K. Shukla , Mukta M. Shukla
IPC分类号： C12Q1/02 , G01N33/68 , C08F10/00 , C08F14/26 , C08B3/06 , C08F112/08 , C08G75/23 , C08F10/06 , C08F10/02 , C08B1/00 , B01J8/00 , C08F212/10
CPC分类号： B01L3/0275 , B01J20/261 , B01J20/28016 , B01J20/28019 , B01J20/282 , B01J20/283 , B01J20/284 , B01J20/285 , B01J2220/445 , B01J2220/64 , B01L2300/0681 , C08L81/06 , G01N30/467 , G01N30/52 , G01N30/54 , G01N30/6091
摘要： A pipette tip or tube containing chromatographic media contained and held in place in said tube by using low melting point porous polymer particles. Such pipette tips or tubes can be used for sample preparation, filtration and synthesis of small molecules and biomolecules.
摘要翻译：含有色谱介质的移液管尖端或管通过使用低熔点多孔聚合物颗粒包含并保持在所述管中的适当位置。这种移液管吸头或管可用于样品制备，过滤和合成小分子和生物分子。

3. 发明授权

US06103195A Micro-volume spin columns for sample preparation 失效
标题翻译：用于样品制备的微量旋转柱
公开(公告)号：US06103195A
公开(公告)日：2000-08-15
申请号：US908931
申请日：1997-08-08
申请人： Ashok K. Shukla , Amita Shukla , Mukta Shukla
发明人： Ashok K. Shukla , Amita Shukla , Mukta Shukla
IPC分类号： G01N30/38 , G01N30/46 , G01N30/52 , G01N30/60 , G01N30/02
CPC分类号： G01N30/38 , G01N2030/381 , G01N2030/527 , G01N30/461 , G01N30/6065
摘要： This invention relates to micro volume spin columns used for the preparation and purification of small chemical and/or biological samples by centrifugation. The present invention relates to the use of the conventional luer lock fitting in micro volume spin columns. This invention also relates to multi-spin column systems based on joining two or more spin columns by their ends or by combining different filtration or ultra-filtration membranes with spin columns.
摘要翻译：本发明涉及用于通过离心制备和纯化小型化学和/或生物样品的微量体积旋转柱。本发明涉及传统的鲁尔锁配件在微体积旋转柱中的应用。本发明还涉及通过其两端连接两个或更多个旋转塔或通过将不同的过滤或超滤膜与旋转塔结合的多旋转塔系统。

4. 发明授权

US5922186A Staining, de-staining and quantification of proteins by coomassie-blue and related dyes 失效
标题翻译：通过考马斯蓝和相关染料染色，去污染和定量蛋白质
公开(公告)号：US5922186A
公开(公告)日：1999-07-13
申请号：US903456
申请日：1997-07-28
申请人： Ashok K. Shukla , Amita M. Shukla
发明人： Ashok K. Shukla , Amita M. Shukla
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44721 , G01N27/44704
摘要： The present invention presents a novel method for both staining electrophoresis gels, as well as removing the background dye during de-staining. This novel and simple pad-based technique minimizes solvent consumption, is environmentally friendly, and does not leave any solid residues on the gel. Furthermore, a novel method for the quantitative and qualitative determination of protein concentration in solution is also presented. This method is simple, easy-to-use and has an easily visible color change from clear to blue in the presence of protein.
摘要翻译：本发明提供了一种染色电泳凝胶的新方法，以及脱色期间去除背景染料。这种新颖简单的基于垫的技术可最大限度地减少溶剂消耗，是环保的，并且不会在凝胶上留下任何固体残留物。此外，还提出了一种定量和定性测定溶液中蛋白质浓度的新方法。这种方法简单，易于使用，在蛋白质存在下可以从透明到蓝色容易地看到颜色的变化。

5. 发明授权

US08124727B2 Use of modified metal oxides for enrichment of phosphopeptides 有权
标题翻译：使用改性金属氧化物富集磷酸肽
公开(公告)号：US08124727B2
公开(公告)日：2012-02-28
申请号：US12288646
申请日：2008-10-22
申请人： Ashok K. Shukla , Mukta M Shukla
发明人： Ashok K. Shukla , Mukta M Shukla
IPC分类号： C07K1/14 , C07K17/00
CPC分类号： C07K1/16 , C07K1/14
摘要： Use of modified metal oxides for the purification and enrichment of negatively charged biomolecules such as peptides, proteins, DNA, RNA, Lipids, carbohydrates, glyco molecules. These metal oxides are modified in such a way that the density of the Lewis acid group is reduced due to modification.
摘要翻译：使用改性金属氧化物来净化和富集带负电荷的生物分子如肽，蛋白质，DNA，RNA，脂质，碳水化合物，糖分子。这些金属氧化物以使路易斯酸基的密度由于改性而降低的方式进行了改性。

6. 发明授权

US5773238A Droplet chemical reaction chamber 失效
标题翻译：液滴化学反应室
公开(公告)号：US5773238A
公开(公告)日：1998-06-30
申请号：US499667
申请日：1995-07-07
申请人： Ashok K. Shukla
发明人： Ashok K. Shukla
IPC分类号： C12N5/00 , C12P1/00 , C12N11/04 , C12N13/00
CPC分类号： B01L3/502792 , B01L3/5088 , C12N5/0068 , B01F13/0071 , B01L2200/16 , B01L2300/165 , C12N2533/30
摘要： A reaction chamber is constructed of a reactant-containing aqueous solution, which may be in droplet form, coated with a fluoropolymer powder such as Polytetrafluoroethylene (PTFE), preferably having a particle size of less than 500 microns. After a reaction, the droplet is destroyed by adding a substance such as a detergent or organic solvent, and the fluoropolymer powder is removed by centrifuging and filtering. Using a micropipette, size of the droplet chamber is increased or decreased by removing or adding liquid, or liquid is transferred from one droplet chamber to another. Charcoal, metal powder or silica powder can be inserted inside or on the surface of the droplet. A droplet chamber containing a first reactant such as an enzyme-bound bead is combined with a second droplet chamber containing a second reactant to react the first and second reactants. A dialysis chamber contains a filtration membrane in contact with the droplet chamber. Placing the droplet chamber between two electrodes and producing current flow between the electrodes provides an electrochemical reaction. A temperature-sensitive reaction such as the Polymerase Chain Reaction (PCR) is performed by heating or cooling the droplet chamber. The droplet reaction chamber is advantageous for microvolumes of sample since container walls are not present on which the sample can disperse, and the chamber does not require significant energy for heating or cooling. In tissue culture, coating a solution containing tissue cells with the fluoropolymer powder reduces sticking of the cells to surfaces of a container.
摘要翻译：反应室由可以是液滴形式的含反应物的水溶液构成，涂覆有氟聚合物粉末，例如聚四氟乙烯（PTFE），优选具有小于500微米的粒度。反应后，通过添加洗涤剂或有机溶剂等物质来破坏液滴，通过离心分离除去含氟聚合物粉末。使用微量移液管，通过去除或添加液体来增加或减少液滴室的尺寸，或者液体从一个液滴室转移到另一液滴室。木炭，金属粉末或二氧化硅粉末可以插入液滴的内部或表面。含有第一反应物如酶结合的珠的液滴室与含有第二反应物的第二液滴室结合以使第一和第二反应物反应。透析室包含与液滴室接触的过滤膜。将液滴室放置在两个电极之间并在电极之间产生电流提供电化学反应。通过加热或冷却液滴室来进行诸如聚合酶链式反应（PCR）的温度敏感反应。液滴反应室对于样品的微量体积是有利的，因为不存在样品可以分散的容器壁，并且室不需要显着的能量用于加热或冷却。在组织培养中，用含氟聚合物粉末涂布含有组织细胞的溶液可以减少细胞对容器表面的粘附。

7. 发明授权

US5745918A Necktie as an information source 失效
标题翻译：领带作为信息来源
公开(公告)号：US5745918A
公开(公告)日：1998-05-05
申请号：US853478
申请日：1997-05-09
申请人： Mukta M. Shukla , Ashok K. Shukla
发明人： Mukta M. Shukla , Ashok K. Shukla
IPC分类号： A41D25/00 , A41D27/08
CPC分类号： A41D25/00 , A41D27/08
摘要： This invention relates to the use of the conventional necktie as a source of information or for communicating a message in text and/or graphic, either by printing, painting, embroidering, or by thermal, chemical, and physical bonding to the surface of the necktie.
摘要翻译：本发明涉及常规领带作为信息来源或通过印刷，绘画，刺绣或通过热，化学和物理粘合到领带的表面来传递文本和/或图形中的消息的用途。

8. 发明授权

US5733442A Microdialysis/Microelectrodialysis system 失效
标题翻译：微透析/微电解透析系统
公开(公告)号：US5733442A
公开(公告)日：1998-03-31
申请号：US225667
申请日：1994-04-11
申请人： Ashok K. Shukla
发明人： Ashok K. Shukla
IPC分类号： B01D57/02 , B01D61/28 , B01D61/42 , B01F13/08 , C12N15/10 , G01N1/34 , G01N27/447 , B01D63/00
CPC分类号： B01D57/02 , B01D61/28 , B01D61/422 , B01D61/425 , B01F13/0818 , C12N15/101 , G01N27/4473 , G01N2001/4016
摘要： A microdialysis system for the dialysis of small sample volumes of protein, nucleic acid, peptide, and other biomolecules has been developed. The device may contain a built-in magnet to permit remote rotation of the device during dialysis. Double sided dialysis chambers can be used for electrodialysis, electroelution, or electroconcentration. Two or more chambers can be joined either by a union or directly for equilibrium dialysis, or other applications.
摘要翻译：已经开发了用于透析小样品体积的蛋白质，核酸，肽和其他生物分子的微透析系统。该装置可以包含内置的磁体，以允许透析期间设备的远程旋转。双面透析室可用于电渗析，电洗脱或电浓缩。两个或更多个室可以通过联合或直接连接用于平衡透析或其它应用。

9. 发明授权

US08034239B2 Device for sample preparation 有权
标题翻译：样品制备装置
公开(公告)号：US08034239B2
公开(公告)日：2011-10-11
申请号：US11985517
申请日：2007-11-15
申请人： Ashok K. Shukla , Mukta Shukla
发明人： Ashok K. Shukla , Mukta Shukla
IPC分类号： B01D15/08
CPC分类号： B01D15/22 , B01J2220/64 , G01N1/405 , G01N1/4077 , G01N2001/4088 , G01N2035/1053
摘要： This invention relates to a pipette tip or tube designed to perform biological or chemical sample preparation, purification or separation. The narrow end of the pipette tip is twisted in such a way that it forms a narrow spiral passage, which allows the retention of the chromatographic material in the pipette tip. The narrow spiral formed by twisting the capillary or the tube creates an opening that is large enough to permit the passage of fluids or smaller particles, but small enough to block the passage of solid materials or larger particles, The device described herein can be used for sample separation or purification using a vacuum, pressure, centrifugation, gravitation or other separation methods.
摘要翻译：本发明涉及一种设计用于进行生物或化学样品制备，纯化或分离的移液器吸头或管。移液管尖端的窄端以这样的方式被扭转，使得其形成窄的螺旋通道，这允许将色谱材料保留在吸头中。通过扭转毛细管或管形成的窄螺旋形成足够大的开口，以允许流体或较小颗粒通过，但足够小以阻止固体材料或较大颗粒的通过。本文所述的装置可用于使用真空，压力，离心，重力或其他分离方法进行样品分离或纯化。

10. 发明申请

US20090269658A1 Grid for Lead-Acid Battery with Electroconductive Polymer Coating 审中-公开
标题翻译：具有导电聚合物涂层的铅酸电池网格
公开(公告)号：US20090269658A1
公开(公告)日：2009-10-29
申请号：US11794585
申请日：2004-12-31
申请人： Ashok K. Shukla , Surendra Kumar Martha , Bellie Hariprakash , Shaik Abdul Gaffoor , Dinesh Chandra Trivedi
发明人： Ashok K. Shukla , Surendra Kumar Martha , Bellie Hariprakash , Shaik Abdul Gaffoor , Dinesh Chandra Trivedi
IPC分类号： H01M4/66 , H01M4/60 , H01M2/02 , C25D5/02
CPC分类号： C09D5/4461 , C25D5/56 , C25D13/04 , H01M4/0402 , H01M4/661 , H01M4/667 , H01M4/73 , H01M10/16 , Y02E60/126 , Y02P70/54
摘要： The present invention provides a method for manufacture of a grid structure for use in lead acid batteries using a substrate having low density and low melting point, the method comprising coating the substrate material with a first metal layer and a subsequent metal layer of lead/lead alloy and electrodepositing an electroconductive polymer to form a protective layer thereon.
摘要翻译：本发明提供一种制造用于使用具有低密度和低熔点的基板的铅酸电池的栅格结构的方法，所述方法包括用第一金属层和随后的铅/铅的金属层涂覆基板材料合金并电沉积导电聚合物以在其上形成保护层。

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