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    • 6. 发明授权
    • Method for accurately enumerating and sensitively qualifying
heterogenous cell populations in cytolytic processing conditions
    • 在细胞溶解处理条件下准确地枚举和敏感鉴定异源细胞群体的方法
    • US5378633A
    • 1995-01-03
    • US197575
    • 1994-02-16
    • Wieland E. von BehrensSherry HaiflichJohn GlazierJohn M. RocheBruce A. Director
    • Wieland E. von BehrensSherry HaiflichJohn GlazierJohn M. RocheBruce A. Director
    • G01N33/49C12M1/34G01N15/14G01N33/50G01N33/48
    • G01N33/5094G01N15/1434G01N15/1459G01N2015/1006G01N2015/1402G01N2015/1413G01N2015/1477Y10T436/107497
    • A method for identifying, characterizing, categorizing and enumerating cells within a cell population. The survivorship characteristics of the different cell populations is used to obtain quantitative and qualitative information about the cell populations initially present in the sample solution before conditions were imposed on the sample solution to elicit a response. The monitored cell survivorship response may be either direct disappearance of intact cells or the appearance of cell structures, carcasses, ghosts or residuum. In a preferred embodiment, a leukocyte cell decay rate in the presence of an erythrolytic agent is determined by monitoring leukocyte counts at several time intervals after the addition of the erythrolytic agent to the sample solution. The leukocyte decay rate is then used to indicate the presence of a fragile leukocyte population, and to accurately estimate the number of leukocytes initially present in the whole blood sample. The use of stronger erythrolytic agents in samples with lyse-resistant erythrocytes while preserving the accuracy of leukocyte counts is allowed. The present method permits calculation of a leukocyte decay rate which can be back extrapolated to time zero to provide an accurate estimate of the leukocyte count initially present in the whole blood sample.
    • 用于识别,表征,分类和枚举细胞群体中的细胞的方法。 不同细胞群体的存活特征用于获得关于样品溶液中最初存在的细胞群的定量和定性信息,然后再对样品溶液施加条件以引发反应。 受监测的细胞存活应答可能是完整细胞的直接消失或细胞结构,胴体,幽灵或残渣的出现。 在优选的实施方案中,在将红细胞溶解剂加入到样品溶液中之后,通过在几个时间间隔监测白细胞计数来确定在存在红血球分解剂的情况下的白血球细胞衰变速率。 然后使用白细胞衰减速率来指示脆性白细胞群的存在,并准确估计最初存在于全血样品中的白细胞数。 允许在具有抗裂红细胞的样品中使用更强的红血球,同时保持白细胞计数的准确性。 本方法允许计算白细胞衰减速率,其可以反向外推到时间零,以提供最初存在于全血样品中的白细胞计数的准确估计。
    • 10. 发明授权
    • Reach-in refrigerated merchandiser
    • 达到冷藏商品
    • US06401399B1
    • 2002-06-11
    • US09676541
    • 2000-09-29
    • John M. RocheJohn A. BehrJohn M. Rasch
    • John M. RocheJohn A. BehrJohn M. Rasch
    • E06B300
    • A47F3/0434E05C17/24E05D7/1011E05D11/06E05Y2900/202E05Y2900/31E06B3/66366E06B3/667F25D23/028F25D23/087
    • A refrigerated, reach-in merchandiser having a product display area with a front opening defined by casing means having at least two mullion members, a reach-in door for closing the front opening and being hingedly mounted on one of the mullion members by door control means, said reach-in door having a transparent panel with a molded frame and at least two glass lites, door control means for hingedly mounting the door on said merchandiser, electric means for said merchandiser including lighting means on one of the mullion members for illuminating the display area, said electric means also including heating means for the glass lites of the transparent panel and including a key member for connecting said heating means to said merchandiser. A thermal door and a method for making the same are also disclosed.
    • 一种具有产品展示区域的冷藏式商品销售商,具有由具有至少两个竖立构件的套管装置限定的前开口,用于封闭前开口并通过门控制铰接安装在竖框构件之一上的入口门 所述入口门具有带有模制框架和至少两个玻璃窗的透明面板,用于将门铰接地安装在所述商品柜上的门控制装置,所述商品销售商的电气装置包括在竖框构件之一上的照明装置,用于照明 所述显示区域,所述电气装置还包括用于所述透明面板的玻璃边缘的加热装置,并且包括用于将所述加热装置连接到所述商品销售器的键构件。 还公开了热门及其制造方法。