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1. 发明申请

US20090101839A1 LASER SCANNER APPARATUS FOR FLUORESCENCE MEASUREMENTS 有权
公开(公告)号：US20090101839A1
公开(公告)日：2009-04-23
申请号：US12252748
申请日：2008-10-16
申请人： ANDREAS ERLBACHER , Harald Gebetsroither , Andreas Gfrorer , Gyoergy Wenczel
发明人： ANDREAS ERLBACHER , Harald Gebetsroither , Andreas Gfrorer , Gyoergy Wenczel
IPC分类号： G02B21/06 , G01N21/64
CPC分类号： G02B21/0076 , G02B5/04 , G02B26/105 , G02B27/141
摘要： The invention relates to a laser scanner apparatus (1) for imaging and/or measuring fluorescent samples which are located on specimen slides (8) and treated with two different fluorescent dyes and also to a corresponding method. This laser scanner apparatus (1) comprises a motor-drivable sample table (2) with a receptacle (34) for specimen slides (8) in a sample plane (49); at least one laser (51,52) and a first optical system (53) for providing two laser beams (54,55) of differing wavelength oriented parallel to each other and extending parallel to this plane (49); a scanner means (72) comprising a scanner head (50) which can move back and forth parallel to this plane (49) and in a direction of movement (75) and has an optical deflection element (56) for deflecting the laser beams (54,55) toward the sample; and a first objective (57) for focusing the laser beams (54,55) on the sample in the plane (49). The laser scanner apparatus (1) additionally comprises a second optical system (58) for forwarding to detectors (61,61′) emission beam bundles (59,60) which are triggered by the laser beams (54,55) on the sample and deflected by the first objective (57) and the deflection element (56) in a direction substantially parallel to the plane (49) and two detectors (61,61′) for detecting the emission beam bundles (59,60) of differing wavelength coming from the samples. The optical deflection element (56) of the laser scanner apparatus (1) according to the invention comprises a wedge-shaped dichroic mirror (62) with front and rear dichroic surfaces (63,64) which are arranged at an intermediate angle (β) to each other. The wedge-shaped dichroic mirror (62) is adjusted in such a way that the two laser beams (54,55) are each reflected at one of the surfaces (63,64). Thus, the wedge-shaped dichroic mirror (62) causes through the intermediate angle (β) a spatial separation of the two resulting focal points (65) and the two emission beam bundles (59,60) guided in the direction of the detectors (61,61′).

2. 发明授权

US08013313B2 Laser scanner apparatus for fluorescence measurements 有权
标题翻译：激光扫描仪用于荧光测量
公开(公告)号：US08013313B2
公开(公告)日：2011-09-06
申请号：US12853484
申请日：2010-08-10
申请人： Andreas Erlbacher , Harald Gebetsroither , Andreas Gfrorer , Gyoergy Wenczel
发明人： Andreas Erlbacher , Harald Gebetsroither , Andreas Gfrorer , Gyoergy Wenczel
IPC分类号： G01N21/64
CPC分类号： G02B21/0076 , G02B5/04 , G02B26/105 , G02B27/141
摘要： Laser scanner and method images or measures fluorescent samples on specimen slides treated with different fluorescent dyes. Motor-driveable sample table for the slides includes a laser and first optical system creating two laser beams of differing wavelength, a scanner with movable head and an optical deflection element for deflecting the beams toward the sample. Scanner has second optical system for forwarding emission beam bundles to two detectors for detecting emission beam bundles coming from the samples. A drive and counter oscillator connected to the drive by connecting rods drives the head for rapid back-and-forth movement and the counter oscillator has a mass at least equivalent to the head for compensation by opposite movement to the head. The optical deflection element causes spatial separation of two resulting focal points and a spatial separation of the two beam bundles in the direction of the detectors.
摘要翻译：激光扫描仪和方法图像或测量用不同荧光染料处理的标本载玻片上的荧光样品。用于滑块的电机可驱动样品台包括激光器和第一光学系统，其产生两个不同波长的激光束，具有可移动头的扫描仪和用于将光束偏转到样品的光学偏转元件。扫描仪具有用于将发射束束转发到两个检测器的第二光学系统，用于检测来自样品的发射束束。通过连杆连接到驱动器的驱动器和反相振荡器驱动头部用于快速的往复运动，并且反相振荡器具有至少相当于头部的质量，用于通过相对于头部的运动进行补偿。光学偏转元件引起两个所产生的焦点的空间分离以及两个束束在检测器的方向上的空间分离。

3. 发明授权

US07777200B2 Laser scanner apparatus for fluorescence measurements 有权
标题翻译：激光扫描仪用于荧光测量
公开(公告)号：US07777200B2
公开(公告)日：2010-08-17
申请号：US12252748
申请日：2008-10-16
申请人： Andreas Erlbacher , Harald Gebetsroither , Andreas Gfrörer , Gyoergy Wenczel
发明人： Andreas Erlbacher , Harald Gebetsroither , Andreas Gfrörer , Gyoergy Wenczel
IPC分类号： G01N21/64
CPC分类号： G02B21/0076 , G02B5/04 , G02B26/105 , G02B27/141
摘要： A laser scanner apparatus images and/or measures a fluorescent sample on a moveable table. At least one laser and an optical system provide two laser beams at differing wavelengths parallel to the fluorescent sample. A moveable wedge-shaped dichroic mirror deflects the laser beams through an objective toward the sample causing spatial separation of the focal points and two non-parallel emission beams. The emission beams are guided through an optical system to two detectors.
摘要翻译：激光扫描仪装置在可移动台上成像和/或测量荧光样品。至少一个激光器和光学系统提供与荧光样品平行的不同波长的两个激光束。可移动的楔形分色镜使激光束通过物镜朝向样品偏转，导致焦点和两个非平行发射光束的空间分离。发射光束通过光学系统被引导到两个检测器。

4. 发明申请

US20100167406A1 MEASURING APPARATUS AND METHOD FOR DETERMINING FLUID PARAMETERS PROVIDED BY A LABORATORY SYSTEM 审中-公开
标题翻译：用于确定实验室系统提供的流体参数的测量装置和方法
公开(公告)号：US20100167406A1
公开(公告)日：2010-07-01
申请号：US12617237
申请日：2009-11-12
申请人： WOLFGANG STREIT , Gerald Probst , Juha Koota , Gyoergy Wenczel
发明人： WOLFGANG STREIT , Gerald Probst , Juha Koota , Gyoergy Wenczel
IPC分类号： G01N31/00 , G01F1/00 , G01L7/00 , G01N33/00 , G01N7/00 , G01N1/00
CPC分类号： B01L3/502 , B01L7/52 , B01L2200/028 , B01L2200/146 , B01L2200/147 , B01L2300/024 , B01L2300/043 , B01L2300/0636 , B01L2300/0663 , B01L2300/0822 , B01L2300/0877 , B01L2400/0487 , Y10T436/25
摘要： Relates to a measuring apparatus (4) and corresponding methods carried out in e.g. hybridization systems for determining fluid parameters provided by a laboratory system (1). The measuring apparatus (4) comprises a measuring unit (5) and a processing unit (6) for determining the fluid parameters provided by the laboratory system (1) and the measuring unit (5) is accomplished to be able to be integrated into this laboratory system (1). The measuring apparatus (4) according to the invention is characterised in that the measuring unit (5) comprises a measuring block (15) without a reaction room (34) and at least one sensor (17). The measuring block (15) comprises hollow spaces (16) for receiving or guiding fluids (13) provided by the laboratory system (1). The at least one sensor (17) is arranged, for determining physical and/or chemical parameters of fluids (13) located in the hollow spaces (16), on or in fluidic operative connection with these hollow spaces (16) of the measuring block (15).
摘要翻译：关于测量装置（4）和相应的方法。用于确定由实验室系统（1）提供的流体参数的杂交系统。测量装置（4）包括测量单元（5）和用于确定由实验室系统（1）提供的流体参数的处理单元（6），并且测量单元（5）被实现为能够集成到该测量单元实验室系统（1）。根据本发明的测量装置（4）的特征在于，测量单元（5）包括没有反应室（34）和至少一个传感器（17）的测量块（15）。测量块（15）包括用于接收或引导由实验室系统（1）提供的流体（13）的中空空间（16）。布置所述至少一个传感器（17），用于确定位于所述中空空间（16）中的流体（13）的物理和/或化学参数，或者与所述测量块的这些中空空间（16）流体地操作连接（15）。

5. 发明授权

US07618812B2 Device and process unit for providing a hybridization chamber 有权
标题翻译：用于提供杂交室的装置和处理装置
公开(公告)号：US07618812B2
公开(公告)日：2009-11-17
申请号：US11191327
申请日：2005-07-28
申请人： Wolfgang Streit , Gyoergy Wenczel , Anton Mathes , Waltraud Lamprecht
发明人： Wolfgang Streit , Gyoergy Wenczel , Anton Mathes , Waltraud Lamprecht
IPC分类号： C12M1/34
CPC分类号： B01L3/502715 , B01F5/10 , B01F11/0071 , B01F13/0059 , B01F15/00123 , B01F15/00318 , B01F15/00396 , B01F15/0244 , B01F2015/00084 , B01F2215/0037 , B01F2215/0073 , B01J2219/00353 , B01J2219/00396 , B01J2219/00477 , B01J2219/00479 , B01J2219/00533 , B01J2219/00659 , B01L3/50273 , B01L3/502738 , B01L3/50855 , B01L9/06 , B01L2200/0689 , B01L2300/0636 , B01L2300/0822 , B01L2300/0877 , B01L2400/0481 , C40B60/14
摘要： A device provides a hybridization chamber for hybridizing nucleic acid samples, proteins or tissue sections on a slide, and is an essentially rectangular body movable opposite the slide. The device includes a surface, lines for supplying and/or removing media, a specimen supply line and an agitating device. The agitation device includes at least one membrane which separates a pressure chamer to be fillable with a pressure fluid via one of the lines, from at least one agitation chamber connected via an agitation line to the hybridization chamber. The lines are continuously open so the media therein can permanently flow unhindered. Four such devices can be combined into a process unit for providing a hybridization chamber. Such unit is pivotable around an axis and has a holder with four seats, which is lockable in relation to a baseplate, one device being insertable into each one of the seats.
摘要翻译：一种装置提供用于将载体上的核酸样品，蛋白质或组织切片杂交的杂交室，并且是与载片相对移动的基本上矩形的主体。该装置包括表面，用于供应和/或移除介质的管线，试样供应管线和搅拌装置。所述搅拌装置包括至少一个膜，其通过所述管线将至少一个通过搅动管线连接的搅拌室与压力流体分离成压缩倒角，所述至少一个搅拌室通过所述杂交室。线路是连续打开的，因此其中的介质可以永久地不受阻碍地流动。四个这样的装置可以组合成用于提供杂交室的处理单元。这种单元可围绕轴线枢转并且具有具有四个座的保持器，该座具可相对于底板锁定，一个装置可插入每个座中。

6. 发明授权

US07615370B2 System having device for preventing air bubbles in a hybridization chamber and corresponding method 有权
标题翻译：具有用于在杂交室中防止气泡的装置的系统和相应的方法
公开(公告)号：US07615370B2
公开(公告)日：2009-11-10
申请号：US10931432
申请日：2004-09-01
申请人： Wolfgang Streit , Gyoergy Wenczel , Waltraud Lamprecht , Heribert Eglauer
发明人： Wolfgang Streit , Gyoergy Wenczel , Waltraud Lamprecht , Heribert Eglauer
IPC分类号： C12M1/34 , C12M3/00
CPC分类号： B01L3/502723 , B01L3/50855 , B01L2200/0684 , B01L2300/0822 , B01L2300/14
摘要： The present invention relates to a system (1) having hybridization chambers (5) for hybridizing nucleic acid samples, proteins, or tissue sections immobilized on slides (27), each hybridization chamber (5) being defined as an essentially gap-shaped chamber, which is essentially fillable with a liquid, between one of these slides (27) and a cover (26), and the cover (26) being positioned in relation to the slide (27) in such a way that the hybridization chamber (5) is sealed to the surrounding air, the system (1) including a device for preventing air bubbles in the hybridization chambers (5). The system according to the present invention is distinguished in that this device for preventing air bubbles in the hybridization chambers (5) is implemented as a pressure device to build up a chamber pressure in the hybridization chambers (5), this chamber pressure lying above the normal atmospheric pressure existing in the surrounding air. The present invention additionally relates to a method for preventing air bubbles in the hybridization chambers (5) of such a system (1) and is distinguished in that, using a pressure device of this system (1), a chamber pressure is implemented in the hybridization chambers (5) which lies above the normal atmospheric pressure existing in the surrounding air.
摘要翻译：本发明涉及一种具有杂交室（5）的系统（1），用于将固定在载片（27）上的核酸样品，蛋白质或组织切片进行杂交，每个杂交室（5）被定义为基本间隙形的室，其基本上可用液体填充，在这些滑动件（27）中的一个和盖（26）之间，并且盖（26）相对于载玻片（27）定位成使得杂交室（5）密封到周围空气，系统（1）包括用于防止杂交室（5）中的气泡的装置。根据本发明的系统的特征在于，用于防止杂交室（5）中的气泡的装置被实施为用于在杂交室（5）中建立室压力的压力装置，该室压力位于周围空气中存在正常的大气压力。本发明还涉及一种用于防止这种系统（1）的杂交室（5）中的气泡的方法，其区别在于，使用该系统（1）的压力装置，在位于周围空气中存在的正常大气压力之上的杂交室（5）。

7. 发明申请

US20060003440A1 Device and process unit for providing a hybridization chamber 有权
公开(公告)号：US20060003440A1
公开(公告)日：2006-01-05
申请号：US11191327
申请日：2005-07-28
申请人： Wolfgang Streit , Gyoergy Wenczel , Anton Mathes , Waltraud Lamprecht
发明人： Wolfgang Streit , Gyoergy Wenczel , Anton Mathes , Waltraud Lamprecht
IPC分类号： C12M1/34
CPC分类号： B01L3/502715 , B01F5/10 , B01F11/0071 , B01F13/0059 , B01F15/00123 , B01F15/00318 , B01F15/00396 , B01F15/0244 , B01F2015/00084 , B01F2215/0037 , B01F2215/0073 , B01J2219/00353 , B01J2219/00396 , B01J2219/00477 , B01J2219/00479 , B01J2219/00533 , B01J2219/00659 , B01L3/50273 , B01L3/502738 , B01L3/50855 , B01L9/06 , B01L2200/0689 , B01L2300/0636 , B01L2300/0822 , B01L2300/0877 , B01L2400/0481 , C40B60/14
摘要： A device provides a hybridization chamber for hybridizing nucleic acid samples, proteins or tissue sections on a slide, and is an essentially rectangular body movable opposite the slide. The device includes a surface, lines for supplying and/or removing media, a specimen supply line and an agitating device. The agitation device includes at least one membrane which separates a pressure chamer to be fillable with a pressure fluid via one of the lines, from at least one agitation chamber connected via an agitation line to the hybridization chamber. The lines are continuously open so the media therein can permanently flow unhindered. Four such devices can be combined into a process unit for providing a hybridization chamber. Such unit is pivotable around an axis and has a holder with four seats, which is lockable in relation to a baseplate, one device being insertable into each one of the seats.

8. 发明申请

US20060008812A1 System having device for preventing air bubbles in a hybridization chamber and corresponding method 有权
标题翻译：具有用于在杂交室中防止气泡的装置的系统和相应的方法
公开(公告)号：US20060008812A1
公开(公告)日：2006-01-12
申请号：US10931432
申请日：2004-09-01
申请人： Wolfgang Streit , Gyoergy Wenczel , Waltraud Lamprecht , Heribert Eglauer
发明人： Wolfgang Streit , Gyoergy Wenczel , Waltraud Lamprecht , Heribert Eglauer
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： B01L3/502723 , B01L3/50855 , B01L2200/0684 , B01L2300/0822 , B01L2300/14
摘要： The present invention relates to a system (1) having hybridization chambers (5) for hybridizing nucleic acid samples, proteins, or tissue sections immobilized on slides (27), each hybridization chamber (5) being defined as an essentially gap-shaped chamber, which is essentially fillable with a liquid, between one of these slides (27) and a cover (26), and the cover (26) being positioned in relation to the slide (27) in such a way that the hybridization chamber (5) is sealed to the surrounding air, the system (1) including a device for preventing air bubbles in the hybridization chambers (5). The system according to the present invention is distinguished in that this device for preventing air bubbles in the hybridization chambers (5) is implemented as a pressure device to build up a chamber pressure in the hybridization chambers (5), this chamber pressure lying above the normal atmospheric pressure existing in the surrounding air. The present invention additionally relates to a method for preventing air bubbles in the hybridization chambers (5) of such a system (1) and is distinguished in that, using a pressure device of this system (1), a chamber pressure is implemented in the hybridization chambers (5) which lies above the normal atmospheric pressure existing in the surrounding air.
摘要翻译：本发明涉及一种具有杂交室（5）的系统（1），用于将固定在载片（27）上的核酸样品，蛋白质或组织切片进行杂交，每个杂交室（5）被定义为基本间隙形的室，其基本上可用液体填充，在这些滑动件（27）中的一个和盖（26）之间，并且盖（26）相对于载玻片（27）定位成使得杂交室（5）密封到周围空气，系统（1）包括用于防止杂交室（5）中的气泡的装置。根据本发明的系统的特征在于，用于防止杂交室（5）中的气泡的装置被实施为用于在杂交室（5）中建立室压力的压力装置，该室压力位于周围空气中存在正常的大气压力。本发明还涉及一种用于防止这种系统（1）的杂交室（5）中的气泡的方法，其区别在于，使用该系统（1）的压力装置，在位于周围空气中存在的正常大气压力之上的杂交室（5）。

9. 发明授权

US07459306B2 Device and method for providing a hybridization chamber and for influencing air bubbles in the same 有权
标题翻译：用于提供杂交室并用于影响其中气泡的装置和方法
公开(公告)号：US07459306B2
公开(公告)日：2008-12-02
申请号：US10909521
申请日：2004-08-02
申请人： Wolfgang Streit , Gyoergy Wenczel , Waltraud Lamprecht , Heribert Eglauer
发明人： Wolfgang Streit , Gyoergy Wenczel , Waltraud Lamprecht , Heribert Eglauer
IPC分类号： C12M1/34 , C12M3/00 , B01L3/00
CPC分类号： B01D19/0021 , B01F13/0059 , B01F2005/0621 , B01L3/502723 , B01L3/502746 , B01L7/52 , B01L2200/0684 , B01L2300/0636 , B01L2300/0822 , B01L2300/0877 , B01L2300/1805 , B01L2400/086
摘要： A device, appropriate process units and/or systems for providing a gap-shaped hybridization space to hybridize nucleic acid samples, proteins or tissue sections over a slide. Said items of device are able to move in relation to the slide and comprise an annular sealing surface to seal the hybridization space by application to a surface of the slide. Said items of device additionally comprise lines to introduce media to and remove them from the hybridization space. Said items of device furthermore comprise a specimen supply line and preferably an agitation device to move fluids in relation to the samples immobilized on the surface of the slide. The devices according to the invention are characterized in that they limit a hybridization space that features relief structures to conduct and/or block air bubbles on at least one surface of the device and the slide directed at the interior of this hybridization space. According to the method of the invention, air bubbles present in the hybridization space are conducted and/or blocked by these relief structures.
摘要翻译：用于提供间隙形杂交空间以在载玻片上杂交核酸样品，蛋白质或组织切片的装置，适当的处理单元和/或系统。所述装置的物品能够相对于滑块移动，并且包括环形密封表面，以通过施加到载玻片的表面来密封杂交空间。所述装置的项目还包括将介质引入杂交空间并将其从杂交空间移除的线。所述装置的物品还包括样品供应管线，优选地是相对于固定在载玻片表面上的样品移动流体的搅拌装置。根据本发明的装置的特征在于，它们限制了杂交空间，该杂交空间具有浮雕结构以在装置的至少一个表面和指向该杂交空间内部的载玻片上传导和/或阻挡气泡。根据本发明的方法，杂交空间中存在的气泡由这些浮雕结构进行和/或阻挡。

10. 发明申请

US20060014269A1 Device and method for providing a hybridization chamber and for influencing air bubbles in the same 有权
标题翻译：用于提供杂交室并用于影响其中气泡的装置和方法
公开(公告)号：US20060014269A1
公开(公告)日：2006-01-19
申请号：US10909521
申请日：2004-08-02
申请人： Wolfgang Streit , Gyoergy Wenczel , Waltraud Lamprecht , Heribert Eglauer
发明人： Wolfgang Streit , Gyoergy Wenczel , Waltraud Lamprecht , Heribert Eglauer
IPC分类号： C12M1/34
CPC分类号： B01D19/0021 , B01F13/0059 , B01F2005/0621 , B01L3/502723 , B01L3/502746 , B01L7/52 , B01L2200/0684 , B01L2300/0636 , B01L2300/0822 , B01L2300/0877 , B01L2300/1805 , B01L2400/086
摘要： A device (1), appropriate process units (18) and/or systems (38) for providing a gap-shaped hybridization space (2) to hybridize nucleic acid samples, proteins or tissue sections over a slide (3). Said items of device (1) are able to move in relation to the slide (3) and comprise an annular sealing surface (4) to seal the hybridization space (2) by application to a surface (5) of the slide (3). Said items of device (1) additionally comprise lines (6,6′) to introduce media to and remove them from the hybridization space (2). Said items of device (1) furthermore comprise a specimen supply line (7) and preferably an agitation device (8) to move fluids in relation to the samples immobilized on the surface (5) of the slide (3). The devices according to the invention are characterized in that they limit a hybridization space (2) that features relief structures (101) to conduct and/or block air bubbles (102) on at least one surface (5,14) of the device (1) and the slide (3) directed at the interior of this hybridization space (2). According to the method of the invention, air bubbles (102) present in the hybridization space (2) are conducted and/or blocked by these relief structures (101).
摘要翻译：一种用于提供间隙形杂交空间（2）的装置（1），适当的处理单元（18）和/或系统（38），以使载体（3）上的核酸样品，蛋白质或组织切片杂交。装置（1）的所述物品能够相对于滑动件（3）移动并且包括环形密封表面（4），以通过施加到滑动件（3）的表面（5）来密封杂交空间（2）。装置（1）的所述物品还包括用于将介质引入杂交空间（2）并将其从杂交空间（2）中移除的线（6,6'）。装置（1）的所述物品还包括样品供应管线（7），优选地相对于固定在载玻片（3）的表面（5）上的样品）移动流体的搅拌装置（8）。根据本发明的装置的特征在于它们限制了具有浮雕结构（101）以在装置的至少一个表面（5,14）上传导和/或阻挡气泡（102）的杂交空间（2） 1）和指向该杂交空间（2）内部的载玻片（3）。根据本发明的方法，存在于杂交空间（2）中的气泡（102）由这些浮雕结构（101）进行和/或阻挡。

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