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1. 发明授权

US4384042A Method and test composition for determination of hydrogen peroxide 失效
标题翻译：用于测定过氧化氢的方法和试验组合物
公开(公告)号：US4384042A
公开(公告)日：1983-05-17
申请号：US288123
申请日：1981-07-29
申请人： Akira Miike , Yoshiaki Shimizu , Toshio Tatano , Katsuyuki Watanabe
发明人： Akira Miike , Yoshiaki Shimizu , Toshio Tatano , Katsuyuki Watanabe
IPC分类号： G01N33/50 , C12Q1/28 , G01N31/00 , G01N33/52 , C12Q1/26 , C12Q1/44 , C12Q1/48 , C12Q1/54 , C12Q1/60 , C12Q1/62
CPC分类号： C12Q1/28 , G01N33/52 , C12Q2326/00 , Y10S435/805 , Y10S435/81
摘要： Disclosed is a method for the colorimetric determination of hydrogen peroxide in a sample by reacting a particular chromogen with the hydrogen peroxide in the presence of peroxidase and measuring the absorbancy of the reaction solution in the visible ray region. Also disclosed is a test composition for carrying out the determination.
摘要翻译：公开了一种通过在过氧化物酶存在下使特定色原体与过氧化氢反应并测量可见光区域中的反应溶液的吸光度的方式来比较测定样品中的过氧化氢的方法。还公开了用于进行测定的测试组合物。

2. 发明授权

US4851353A Method and test composition for determination of lipid peroxide 失效
标题翻译：测定脂质过氧化物的方法和试验组合物
公开(公告)号：US4851353A
公开(公告)日：1989-07-25
申请号：US11401
申请日：1987-02-04
申请人： Akira Miike , Yoshiaki Shimizu , Toshio Tatano , Katsuyuki Watanabe
发明人： Akira Miike , Yoshiaki Shimizu , Toshio Tatano , Katsuyuki Watanabe
IPC分类号： G01N33/50 , C12Q1/00 , C12Q1/28 , G01N31/00 , G01N33/52 , G01N33/72
CPC分类号： C12Q1/28 , G01N33/52 , G01N33/72 , C12Q2326/00 , Y10S436/904 , Y10T436/19 , Y10T436/193333 , Y10T436/206664
摘要： A method for the determination of peroxide in a sample is disclosed which comprises reacting the peroxide with a chromogen represented by the general formula (I) or (II) ##STR1## wherein R.sub.1 and R.sub.3 represent amino, mono- or di- substituted amino, hydroxyl or hydroxyalkyl, R.sub.4 and R.sub.5 represent hydrogen, alkyl, alkylene, acyl, halogen, sulphone, nitro, carboxyl, hydroxyl or hydroxyalkyl, R.sub.2 represents hydrogen, ##STR2## wherein R.sub.6 represents hydrogen, alkyl, aralkyl, alkylene, aryl or mono- or di- substituted aryl, and --Z-- may change to --Z.dbd. by resonance and represents --S--, --O--, --N.dbd., ##STR3## wherein R.sub.7, R.sub.8, R.sub.9 and R.sub.10 have the same significance as R.sub.6 in the presence of heme compound, iodide or bromide and measuring the absorbancy of the reaction solution in the visible ray region. Also disclosed is a test composition for the determination of peroxide which comprises a chromogen as defined above and a compound selected from a heme compound, iodide and bromide.
摘要翻译：公开了一种用于测定样品中过氧化物的方法，其包括使过氧化物与由通式（I）或（II）通式（I）表示的发色原反应：通式（II）：其中R1和R3代表氨基，单取代或二取代的氨基，羟基或羟基烷基，R4和R5代表氢，烷基，亚烷基，酰基，卤素，砜，硝基，羧基，羟基或羟烷基，R2代表氢，其中R 6表示氢，烷基，芳烷基，亚烷基，芳基或单取代或二取代的芳基，-Z-可以通过共振转变为-Z =，表示-S-，-O-，-N =，其中R7，R8，R9和R10在血红素化合物，碘化物或溴化物的存在下与R6具有相同的意义，并测量可见光区域中的反应溶液的吸光度。还公开了用于测定过氧化物的测试组合物，其包含如上定义的色原和选自血红素化合物，碘化物和溴化物的化合物。

3. 发明授权

US4978615A Method for the determination of the compound having mercapto group 失效
标题翻译：测定具有巯基的化合物的方法
公开(公告)号：US4978615A
公开(公告)日：1990-12-18
申请号：US157141
申请日：1988-02-10
申请人： Norihito Aoyama , Akira Miike , Yoshiaki Shimizu , Toshio Tatano
发明人： Norihito Aoyama , Akira Miike , Yoshiaki Shimizu , Toshio Tatano
IPC分类号： G01N33/50 , C12Q1/26 , C12Q1/28 , G01N33/68
CPC分类号： C12Q1/26 , C12Q1/28 , C12Q2326/00 , Y10S435/81
摘要： The present invention provides a method for the determination of a compound having mercapto group which comprises reacting the compound with a chromogen represented by the following general formula (I): ##STR1## wherein Y is hydrogen or hydroxyl; R.sub.1, R.sub.2 and R.sub.3 may be the same or different, and are groups represented by the following general formula (II), (III) or (IV): ##STR2## wherein Z (which may be the same or different) is hydroxyl, amino, substituted amino, alkyl, substituted alkyl, alkenyl, aryl, substituted aryl, acyl, halogen, nitro, sulfo, carboxyl or alkoxy; n is 0, 1, 2, or 3; provided that at least one Z in R.sub.1, R.sub.2 and R.sub.3 is hydroxyl, amino, or substituted amino, in the presence of peroxidase or thiol oxide reductase, and determining the pigment thus formed.
摘要翻译：本发明提供了一种用于测定具有巯基的化合物的方法，该方法包括使化合物与由以下通式（I）表示的色原体反应：其中Y为氢或羟基; （II），（III）或（IV）表示的基团可以相同或不同，R1，R2和R3可以相同或不同，并且是由以下通式（II），（III）或（IV）表示的基团：）其中Z（其可以相同或不同）为羟基，氨基，取代的氨基，烷基，取代的烷基，烯基，芳基，取代的芳基，酰基，卤素，硝基，磺基，羧基或烷氧基; n为0,1,2或3; 条件是在过氧化物酶或硫醇氧化物还原酶的存在下，R1，R2和R3中的至少一个Z是羟基，氨基或取代的氨基，并且确定如此形成的颜料。

4. 发明授权

US4824779A Method for the determination of the reduced form of nicotinamide adenine dinucleotide 失效
标题翻译：测定烟酰胺腺嘌呤二核苷酸还原形式的方法
公开(公告)号：US4824779A
公开(公告)日：1989-04-25
申请号：US706404
申请日：1985-02-28
申请人： Norihito Aoyama , Akira Miike , Yoshiaki Shimizu , Toshio Tatano
发明人： Norihito Aoyama , Akira Miike , Yoshiaki Shimizu , Toshio Tatano
IPC分类号： G01N33/50 , C12Q1/00 , C12Q1/26 , C12Q1/28
CPC分类号： C12Q1/008 , C12Q1/28 , C12Q2326/00
摘要： The present invention provides a method for the determination of NAD(P)H which comprises reacting a chromogen represented by the following general formula (I): ##STR1## wherein Y is hydrogen or hydroxyl; R.sub.1, R.sub.2 and R.sub.3 may be the same or different, and are groups represented by the following general formula (II), (III) or (IV): ##STR2## wherein Z is hydrogen, hydroxyl, amino, substituted amino, alkyl, substituted alkyl, alkenyl, aryl, substituted aryl, acyl, halogen, nitro, sulfo, carboxyl or alkoxy; n is 1, 2, or 3; provided that at least one Z in R.sub.1, R.sub.2 and R.sub.3 is hydroxyl, amino, or substituted amino; and Z.sub.s in Z.sub.n may be the same or different, with NAD(P)H in the presence of (1) peroxidase or thiol oxide reductase and (2) diaphorase or an electron carrier, and determining the pigment thus formed.
摘要翻译：本发明提供了一种测定NAD（P）H的方法，该方法包括使由以下通式（I）表示的色原反应：其中Y为氢或羟基; （II），（III）或（IV）表示的基团可以相同或不同，R1，R2和R3可以相同或不同，并且是由以下通式（II），（III）或（IV）表示的基团：）其中Z是氢，羟基，氨基，取代的氨基，烷基，取代的烷基，烯基，芳基，取代的芳基，酰基，卤素，硝基，磺基，羧基或烷氧基; n为1,2或3; 条件是R 1，R 2和R 3中的至少一个Z是羟基，氨基或取代的氨基; 并且在（1）过氧化物酶或硫醇氧化物还原酶和（2）心肌黄酶或电子载体的存在下，Zn中的Zs可以相同或不同，具有NAD（P）H，并且确定如此形成的颜料。

5. 发明授权

US4916058A Method and test composition for determination of hydrogen peroxide 失效
标题翻译：用于测定过氧化氢的方法和试验组合物
公开(公告)号：US4916058A
公开(公告)日：1990-04-10
申请号：US65126
申请日：1987-06-23
申请人： Norihito Aoyama , Akira Miike , Yoshiaki Shimizu , Toshio Tatano
发明人： Norihito Aoyama , Akira Miike , Yoshiaki Shimizu , Toshio Tatano
IPC分类号： G01N33/50 , C12Q1/28 , G01N31/00 , G01N31/22
CPC分类号： G01N31/22 , C12Q1/28 , C12Q2326/00 , Y10S435/81
摘要： Disclosed is a method for the colorimetric determination of hydrogen peroxide in a sample by reacting a particular chromogen with the hydrogen perioxide in the presence of peroxidase and measuring the absorbancy of the reaction solution in the visible or near inflared ray region. Also disclosed is a test composition for carrying out the determination.
摘要翻译：公开了通过在过氧化物酶的存在下使特定的色原体与氢氧化铂反应并测量可见光或近似辐射的光线区域中的反应溶液的吸光度来比较测定样品中过氧化氢的方法。还公开了用于进行测定的测试组合物。

6. 发明授权

US4416982A Composition and method for decomposing hydrogen peroxide 失效
标题翻译：用于分解过氧化氢的组合物和方法
公开(公告)号：US4416982A
公开(公告)日：1983-11-22
申请号：US321603
申请日：1981-11-16
申请人： Mitsuru Tsuda , Akira Miike , Yoshiaki Shimizu , Yasuharu Yokote , Toshio Tatano
发明人： Mitsuru Tsuda , Akira Miike , Yoshiaki Shimizu , Yasuharu Yokote , Toshio Tatano
IPC分类号： C01B15/01 , C12P1/00 , C12P3/00 , C12Q1/28 , C12Q1/60 , C12Q1/00 , C12Q1/26 , C12Q1/40 , C12Q1/42 , C12Q1/44 , C12Q1/52 , C12Q1/54
CPC分类号： C12Q1/28 , C12Q2326/00 , C12Q2326/96
摘要： A method for decomposing hydrogen peroxide is disclosed. The hydrogen peroxide is reacted with a compound represented by the formula (I) ##STR1## wherein Z represents OH or NR.sub.4.R.sub.5 wherein R.sub.4 and R.sub.5 are the same or different and represent hydrogen, alkyl, substituted alkyl or acyl, and R.sub.1,R.sub.2 and R.sub.3 are the same or different and represent hydrogen, halogen, alkyl, alkoxy, amino, nitro, carboxyl or sulphonyl, in the presence of peroxidase.
摘要翻译：公开了一种分解过氧化氢的方法。过氧化氢与式（I）表示的化合物反应，其中Z表示OH或NR4R5，其中R4和R5相同或不同，代表氢，烷基，取代的烷基或酰基，以及在过氧化物酶存在下，R 1，R 2和R 3相同或不同，代表氢，卤素，烷基，烷氧基，氨基，硝基，羧基或磺酰基。

7. 发明授权

US4877727A Substrate for determining leucine aminopeptidase or gamma-glutamyltranspeptase activity 失效
标题翻译：用于测定亮氨酸氨基肽酶或γ-谷氨酰转肽酶活性的底物
公开(公告)号：US4877727A
公开(公告)日：1989-10-31
申请号：US248347
申请日：1988-09-20
申请人： Akira Miike , Yukio Katsumata , Toshio Tatano
发明人： Akira Miike , Yukio Katsumata , Toshio Tatano
IPC分类号： C12Q1/37 , C12Q1/48
CPC分类号： C12Q1/48 , C12Q1/37 , C12Q2337/10
摘要： Leucine aminopeptidase (LAP) activity or .gamma.-glutamyltranspeptidase (.gamma.-GTP) activity in a sample is determined by converting a substrate represented by the formula (I): ##STR1## wherein Z represents (CH.sub.3).sub.2 CHCH.sub.2 CH(NH.sub.2)--or (NH.sub.2)(COOH) CHCH.sub.2 CH.sub.2 --, R.sub.1 represents hydrogen, alkyl or substituted alkyl, R.sub.2 represents alkylene or hydroxyalkylene, R.sub.3 and R.sub.4 are the same or different and represents hydrogen, halogen, nitro, hydroxyl, sulfo, carboxyl, alkyl, and alkoxy, and salts thereof into a compound represented by the formula (II): ##STR2## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4 have the same meanings as defined above. The compound (II) is reacted with a chromogen to form a pigment or converted into a diazonium salt which is reacted with a coupling agent to form an azo dye. Absorption of a resultant colored reaction solution is measured.
摘要翻译：通过转化由式（I）表示的底物来确定样品中的亮氨酸氨基肽酶（LAP）活性或γ-谷氨酰转肽酶（γ-GTP）活性：其中Z表示（CH 3）2 CHCH 2 CH（NH 2） - 或（NH 2）（COOH）CHCH 2 CH 2 - ，R 1表示氢，烷基或取代的烷基，R 2表示亚烷基或羟基亚烷基，R 3和R 4相同或不同，表示氢，卤素，硝基，羟基，磺基，羧基，烷基和（II）表示的化合物：其中R 1，R 2，R 3和R 4具有与上述相同的含义。使化合物（II）与色原反应形成颜料或转化为重氮盐，其与偶联剂反应以形成偶氮染料。测量所得有色反应溶液的吸收。

8. 发明授权

US5302513A Method for determination of components 失效
标题翻译：元件测定方法
公开(公告)号：US5302513A
公开(公告)日：1994-04-12
申请号：US727369
申请日：1991-07-09
申请人： Akira Miike , Toshio Tatano
发明人： Akira Miike , Toshio Tatano
IPC分类号： C12Q1/00 , C12Q1/40 , C12Q1/48 , C12Q1/26 , C12Q1/37 , C12Q1/52
CPC分类号： C12Q1/48 , C12Q1/008 , C12Q1/40
摘要： The present invention relates to a method for quantitative determination of NAD(P)H derived from a specific component, which comprises converting NAD(P)H present in a sample or formed from components other than the objective component by reactions into NAD(P) by the action of glutathione of oxidation type and glutathione reductase; decomposing the remaining glutathione of oxidation type by the action of .gamma.-glutamyl transpeptidase in the presence or in the absence of a mercapto compound; forming NAD(P)H from the component to be determined in the sample utilizing an NAD(P)H-forming reaction system; and quantitatively determining NAD(P)H.According to the method of the present invention, amylase activity, etc. in vital components containing maltose and glucose can be determined accurately.
摘要翻译：本发明涉及一种定量测定由特定组分衍生的NAD（P）H的方法，该方法包括将样品中存在的NAD（P）H或通过反应成NAD（P）的目标成分以外的成分形成NAD 通过氧化型和谷胱甘肽还原酶的谷胱甘肽的作用; 在存在或不存在巯基化合物的情况下，通过（γ） - 谷氨酰转肽酶的作用分解剩余的氧化型谷胱甘肽; 使用形成NAD（P）H的反应体系从样品中确定的组分形成NAD（P）H; 并定量测定NAD（P）H。根据本发明的方法，可以准确地测定含有麦芽糖和葡萄糖的重要成分中的淀粉酶活性等。

9. 发明授权

US5196312A Method and test composition for determination of enzyme activity 失效
标题翻译：用于测定酶活性的方法和测试组合物
公开(公告)号：US5196312A
公开(公告)日：1993-03-23
申请号：US270676
申请日：1988-11-14
申请人： Akira Miike , Toshio Tatano
发明人： Akira Miike , Toshio Tatano
IPC分类号： G01N21/75 , C12Q1/00 , C12Q1/26 , C12Q1/34 , C12Q1/37 , C12Q1/40 , C12Q1/42 , C12Q1/48 , C12Q1/56 , G01N21/59
CPC分类号： C12Q1/56 , C12Q1/26 , C12Q1/37 , C12Q1/40 , C12Q1/42 , C12Q2334/00 , G01N2333/96444 , G01N2333/96455 , G01N2333/968 , G01N2333/974
摘要： Disclosed is a method for determination of an activity of .gamma.-glutamyl transpeptidase, leucine aminopeptidase, alanine aminopeptidase, cystine aminopeptidase, X factor as a coagulation factor, thrombin, plasmin of plasminogen series, kallikrein, chymotrypsin, alkali phosphatase, N-acetyl glucosaminase and amylase, by allowing a particular substrate to act on the enzyme to thereby form an enhancer; oxidizing a chromogen by an oxidase in the presence of the enhancer and oxygen to form a pigment; and determining the pigment. Also disclosed is a test composition for carrying out the determination.

10. 发明授权

US5223404A Composition for testing periodontal diseases 失效
标题翻译：牙周病检测组成
公开(公告)号：US5223404A
公开(公告)日：1993-06-29
申请号：US639742
申请日：1991-01-11
申请人： Hirohisa Suido , Akira Miike , Kenji Hasegawa , Norihiko Kayahara , Toru Eguchi , Toshio Tatano , Koichi Nakashima
发明人： Hirohisa Suido , Akira Miike , Kenji Hasegawa , Norihiko Kayahara , Toru Eguchi , Toshio Tatano , Koichi Nakashima
IPC分类号： C12Q1/26 , C12Q1/37
CPC分类号： C12Q1/26 , C12Q1/37 , C12Q2334/00 , Y10S435/81 , Y10S530/802
摘要： A composition for testing periodontal diseases which diagnoses or prognosticates contraction or progress of the diseases or diagnoses the therapeutic value by promptly determining peptidase-like enzymatic activity in a specimen. The composition is a combination of a compound of the formula [1] or [2] or a mixture thereof, a chromogen and an oxidase:X-T-Pro-Y [1]orX'-Z'-Arg-Y' [2]wherein Pro is proline residue; Arg is arginine residue; X and X' are hydrogen or an amino protecting group, respectively; Y and Y' are a residue of a compound which can increase oxidation reaction rate of a chromogen with a oxidase in the presence of oxygen and is attached to the C-terminal of Pro or Arg, respectively; and T and Z' are an amino acid or peptide residue composed of 0 to 4 amino acids or their protected derivatives the C-terminal of which is attached to the N-terminal of Pro or Arg, respectively.
摘要翻译：用于测定牙周疾病的组合物，其通过迅速测定样品中的肽酶样酶活性来诊断或预测疾病的收缩或进展或诊断治疗价值。该组合物是式[1]或[2]的化合物或其混合物，色原和氧化酶：XT-Pro-Y [1]或X'-Z'-Arg-Y'[2 ]其中Pro为脯氨酸残基; Arg是精氨酸残基; X和X'分别为氢或氨基保护基; Y和Y'是化合物的残基，其可以在氧的存在下与氧化酶一起增加色原体的氧化反应速率，并分别连接到Pro或Arg的C-末端; 并且T和Z'是由0至4个氨基酸组成的氨基酸或肽残基或其C末端分别连接到Pro或Arg的N末端的受保护的衍生物。

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