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    • 5. 发明授权
    • Processes for producing coenzyme Q10
    • 生产辅酶Q10的方法
    • US09315839B2
    • 2016-04-19
    • US13020500
    • 2011-02-03
    • Kazuyoshi YajimaTakahisa KatoAkihisa KandaShiro KitamuraYasuyoshi Ueda
    • Kazuyoshi YajimaTakahisa KatoAkihisa KandaShiro KitamuraYasuyoshi Ueda
    • C12P7/66C12P7/22
    • C12P7/66C12P7/22
    • The present invention relates to a process for producing reduced coenzyme Q10 which comprises obtaining microbial cells containing reduced coenzyme Q10 at a ratio of not less than 70 mole % among the entire coenzymes Q10, optionally disrupting the cells and recovering thus produced reduced coenzyme Q10. The present invention also relates to a process for producing oxidized coenzyme Q10 which comprises either recovering oxidized coenzyme Q10 after oxidizing the above-mentioned microbial cells or disrupted product thereof, or recovering reduced coenzyme Q10 from the above-mentioned microbial cells or disrupted product thereof to oxidize thus-obtained reduced coenzyme Q10 thereafter. According to the processes of the present invention, reduced coenzyme Q10 and oxidized coenzyme Q10 can be produced simply on the industrial scale.
    • 本发明涉及一种还原型辅酶Q10的制备方法,其包括在整个辅酶Q10中获得含有还原型辅酶Q10比例不小于70摩尔%的微生物细胞,任选地破坏细胞并回收所生成的还原型辅酶Q10。 本发明还涉及氧化型辅酶Q10的制造方法,该方法包括在氧化上述微生物细胞或其破坏的产物后回收氧化型辅酶Q10,或将还原型辅酶Q10从上述微生物细胞或其破坏性产物回收至 此后氧化由此得到的还原型辅酶Q10。 根据本发明的方法,可以简单地在工业规模上制备还原型辅酶Q10和氧化型辅酶Q10。
    • 6. 发明授权
    • Method for preserving reduced coenzyme Q10
    • 保留还原型辅酶Q10的方法
    • US08067217B2
    • 2011-11-29
    • US11315161
    • 2005-12-23
    • Takahiro UedaTadao OnoShiro KitamuraYasuyoshi Ueda
    • Takahiro UedaTadao OnoShiro KitamuraYasuyoshi Ueda
    • C12N9/98
    • A61K9/4816
    • The present invention has its object to provide a method for stably preserving a capsule containing reduced coenzyme Q10, which is useful as foods, functional nutritive foods, specific health foods, nutritional supplements, nutrients, animal drugs, drinks, feeds, cosmetics, medicines, remedies, preventive drugs, etc. The present invention relates to a method for preserving reduced coenzyme Q10 which comprises producing or obtaining a capsule containing reduced coenzyme Q10, and controlling environment surrounding the capsule to a relative humidity of not less than 0% but not more than 60%. According to this method, reduced coenzyme Q10 can be preserved stably, without requiring huge cost and labor, or special equipment.
    • 本发明的目的是提供一种用于稳定保存含有还原型辅酶Q10的胶囊的方法,所述还原型辅酶Q10可用作食品,功能营养食品,特定保健食品,营养补充剂,营养物质,动物药物,饮料,饲料,化妆品,药​​物, 补救剂,预防药物等。本发明涉及一种保存还原型辅酶Q10的方法,其包括制备或获得含有还原型辅酶Q10的胶囊,并且将胶囊周围的环境控制在相对湿度不小于0%但不超过 超过60%。 根据该方法,可以稳定地保持还原型辅酶Q10,而不需要巨大的成本和劳动力,或特殊设备。
    • 10. 发明授权
    • Method of collecting highly pure polyhydroxyalkanoate from microbial cells
    • 从微生物细胞中收集高纯度聚羟基链烷酸酯的方法
    • US07393668B2
    • 2008-07-01
    • US10541389
    • 2004-01-20
    • Yoshifumi YanagitaNoriko OgawaYasuyoshi UedaFumio OsakadaKeiji Matsumoto
    • Yoshifumi YanagitaNoriko OgawaYasuyoshi UedaFumio OsakadaKeiji Matsumoto
    • C12P7/62C12N9/00
    • C12P7/625
    • The present invention has an object to provide a method for separating and purifying a PHA without causing a serious decrease of the molecular weight to obtain a highly pure PHA in a high yield, which comprises efficiently removing cell components other than PHA particles from a cultured PHA-containing microbial cell. Another object of the present invention is to provide a method for obtaining an agglomerate of PHA particles.The method for recovering a PHA according to the present invention is a method which comprises efficiently disrupting a cell to recover the PHA by carrying out a physical disruption treatment and an alkali addition at low temperature for an aqueous suspension of the PHA-containing microbial cell, and then treating the PHA with an enzyme and/or a surfactant. Moreover, the particle diameter of the PHA may be enlarged by suspending the PHA in a hydrophilic solvent and/or water, and stirring at a temperature equal to or below the boiling point of said suspension, to agglomerate said PHA.
    • 本发明的目的是提供一种分离纯化PHA的方法,而不会导致分子量的严重降低以获得高产率的高纯度PHA,其包括从培养的PHA有效除去PHA颗粒以外的细胞成分 含微生物细胞。 本发明的另一个目的是提供一种获得PHA颗粒附聚物的方法。 根据本发明的回收PHA的方法是通过对含PHA的微生物细胞的水性悬浮液进行物理破坏处理和低温碱添加来有效地破坏细胞以回收PHA的方法, 然后用酶和/或表面活性剂处理PHA。 此外,通过将PHA悬浮在亲水溶剂和/或水中并在等于或低于所述悬浮液沸点的温度下搅拌以使所述PHA聚集,可以扩大PHA的粒径。