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    • 4. 发明授权
    • Cell culture vessel and cultured cell
    • 细胞培养容器和培养细胞
    • US07655457B2
    • 2010-02-02
    • US10989910
    • 2004-11-17
    • Kosuke KuwabaraAkihiro MiyauchiMasahiko OginoTakashi Ando
    • Kosuke KuwabaraAkihiro MiyauchiMasahiko OginoTakashi Ando
    • C12M1/14C12M3/04C12M3/00C12M1/00
    • C12M23/20C12M21/08C12M23/10
    • It is an object of this invention to provide a cell culture vessel which has a simple and convenient structure, can prevent the injury on the cells at the time of peeling, and accelerates the transportation of nutrients and discharge of waste materials. In order to solve the problem mentioned above, the cell culture vessel of this invention has protrusions having a corresponding diameter not smaller than 10 nm and not greater than 10 μm and a height not smaller than 10 nm and not greater than 1 mm on the surface of the cell culture vessel. The protrusions make the culture fluid permeate into the under part of the cells, accelerate the supply of nutrients and discharge of waste materials, and makes the contact between the cells and the vessel as a point-contact and thereby prevents the cells from the injury which the cells undergo at the time of peeling.
    • 本发明的目的是提供一种结构简单方便的细胞培养容器,可以防止剥离时对细胞的损伤,加速营养物质的运输和废料排出。 为了解决上述问题,本发明的细胞培养容器具有相对应的直径不小于10nm且不大于10um的突起,并且在表面上具有不小于10nm且不大于1mm的高度 的细胞培养容器。 突起使得培养液渗透到细胞的下部,加速营养物质的供给和排出废物,并使细胞与血管之间的接触作为点接触,从而防止细胞受损, 细胞在剥离时经历。
    • 8. 发明申请
    • Imprint device and microstructure transfer method
    • 压印装置和微结构转印方法
    • US20060286193A1
    • 2006-12-21
    • US11438336
    • 2006-05-23
    • Takashi AndoSusumu KomoriyaMasahiko OginoChiseki HaginoyaAkihiro Miyauchi
    • Takashi AndoSusumu KomoriyaMasahiko OginoChiseki HaginoyaAkihiro Miyauchi
    • B28B11/08
    • G03F7/0002B82Y10/00B82Y40/00G03F9/703G03F9/7088
    • In an imprint device and a microstructure transfer method, a fluid is ejected at the back of at least either a stamper or a transfer target body during pressurization of the stamper and the transfer target body. The fluid is ejected through plural holes formed in a stage disposed at the back of at least either the stamper or the transfer target body. The plural holes are connected to independent pressure regulating mechanisms, which can individually control the amount of fluid ejection, the timing of start of ejection, and so on. When the stamper is peeled from the transfer target body, the plural holes are evacuated to fix the stamper or the transfer target body to the stage by suction so as to peel the stamper. The present invention enables to apply uniform pressure to the stamper against the surface of the target substrate, to control the in-plane pressure distribution according to the surface profile or external appearance of the stamper or the target substrate, and to peel the stamper from the target substrate immediately after pressurization.
    • 在压印装置和微结构转印方法中,在压模和转印体的加压期间,在至少压模或转印体的至少一个的背面喷射流体。 流体通过形成在设置在压模或转印体中的至少一个的背面的台中的多个孔喷出。 多个孔连接到独立的压力调节机构,其可以单独控制流体喷射的量,喷射开始的时间等。 当压模从转印体上剥离时,将多个孔抽真空,通过抽吸将压模或转印目标体固定在平台上,从而剥离压模。 本发明能够对压模施加均匀的压力以抵抗目标基板的表面,以根据压模或目标基板的表面轮廓或外观来控制平面内的压力分布,并且将压模从 加压后立即进行目标基材。