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    • 2. 发明授权
    • Cell culture vessel and cultured cell
    • 细胞培养容器和培养细胞
    • US07655457B2
    • 2010-02-02
    • US10989910
    • 2004-11-17
    • Kosuke KuwabaraAkihiro MiyauchiMasahiko OginoTakashi Ando
    • Kosuke KuwabaraAkihiro MiyauchiMasahiko OginoTakashi Ando
    • C12M1/14C12M3/04C12M3/00C12M1/00
    • C12M23/20C12M21/08C12M23/10
    • It is an object of this invention to provide a cell culture vessel which has a simple and convenient structure, can prevent the injury on the cells at the time of peeling, and accelerates the transportation of nutrients and discharge of waste materials. In order to solve the problem mentioned above, the cell culture vessel of this invention has protrusions having a corresponding diameter not smaller than 10 nm and not greater than 10 μm and a height not smaller than 10 nm and not greater than 1 mm on the surface of the cell culture vessel. The protrusions make the culture fluid permeate into the under part of the cells, accelerate the supply of nutrients and discharge of waste materials, and makes the contact between the cells and the vessel as a point-contact and thereby prevents the cells from the injury which the cells undergo at the time of peeling.
    • 本发明的目的是提供一种结构简单方便的细胞培养容器,可以防止剥离时对细胞的损伤,加速营养物质的运输和废料排出。 为了解决上述问题,本发明的细胞培养容器具有相对应的直径不小于10nm且不大于10um的突起,并且在表面上具有不小于10nm且不大于1mm的高度 的细胞培养容器。 突起使得培养液渗透到细胞的下部,加速营养物质的供给和排出废物,并使细胞与血管之间的接触作为点接触,从而防止细胞受损, 细胞在剥离时经历。
    • 4. 发明申请
    • Cell culture vessel and cultured cell
    • 细胞培养容器和培养细胞
    • US20050214935A1
    • 2005-09-29
    • US10989910
    • 2004-11-17
    • Kosuke KuwabaraAkihiro MiyauchiMasahiko OginoTakashi Ando
    • Kosuke KuwabaraAkihiro MiyauchiMasahiko OginoTakashi Ando
    • C12M1/14C12M3/00C12M3/04
    • C12M23/20C12M21/08C12M23/10
    • It is an object of this invention to provide a cell culture vessel which has a simple and convenient structure, can prevent the injury on the cells at the time of peeling, and accelerates the transportation of nutrients and discharge of waste materials. In order to solve the problem mentioned above, the cell culture vessel of this invention has protrusions having a corresponding diameter not smaller than 10 nm and not greater than 10 μm and a height not smaller than 10 nm and not greater than 1 mm on the surface of the cell culture vessel. The protrusions make the culture fluid permeate into the under part of the cells, accelerate the supply of nutrients and discharge of waste materials, and makes the contact between the cells and the vessel as a point-contact and thereby prevents the cells from the injury which the cells undergo at the time of peeling.
    • 本发明的目的是提供一种结构简单方便的细胞培养容器,可以防止剥离时对细胞的损伤,加速营养物质的运输和废料排出。 为了解决上述问题,本发明的细胞培养容器具有相对应的直径不小于10nm且不大于10um的突起,并且在表面上具有不小于10nm且不大于1mm的高度 的细胞培养容器。 突起使得培养液渗透到细胞的下部,加速营养物质的供给和排出废物,并使细胞与血管之间的接触作为点接触,从而防止细胞受损, 细胞在剥离时经历。