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1. 发明申请

US20050257281A1 Method for preparing a sterile transgenic fish 失效
标题翻译：无菌转基因鱼的制备方法
公开(公告)号：US20050257281A1
公开(公告)日：2005-11-17
申请号：US11094286
申请日：2005-03-31
申请人： Wei Hu , Zuoyan Zhu , Yaping Wang , Shuangfei Li
发明人： Wei Hu , Zuoyan Zhu , Yaping Wang , Shuangfei Li
IPC分类号： A01K61/00 , A01K67/027 , C07K14/46
CPC分类号： C07K14/461 , A01K67/0275 , A01K2217/05 , A01K2227/40 , A01K2267/02
摘要： The present application disclosed a method for preparing a sterile transgenic fish, comprising constructing antisense RNA expression vector of salmon-type gonadotropin-releasing hormone, introducing the recombinant DNA fragment into carp oosperm by microinjection, and screening the sterile transgenic fish by Polymerase Chain Reaction and radioimmunoassay, wherein the expression vector comprising a promoter of carp beta actin (β-actin) gene, a complementary DNA fragment of antisense salmon-type gonadotropin-releasing hormone (sGnRH) gene from carp with 323 bp as a target gene comprising sGnRH decapeptide, the coding region of gonadotropin-releasing hormone associated peptide and 3′ non-coding sequence, and 3′ flanking sequence of grass carp growth hormone gene as a stop sequence. The method of the present invention is easy and convenient for operation which provides a basis for providing a technical platform of general applicability for solving the hereditary and ecological safety problems of transgenic fishes.
摘要翻译：本申请公开了一种制备无菌转基因鱼的方法，包括构建鲑鱼型促性腺激素释放激素的反义RNA表达载体，通过显微注射将重组DNA片段引入鲤鱼胚乳，并通过聚合酶链式反应筛选无菌转基因鱼，放射免疫测定法，其中包含鲤鱼β肌动蛋白（β-肌动蛋白）基因启动子的表达载体，具有323bp作为靶基因的来自鲤鱼的反义鲑鱼型促性腺激素释放激素（sGnRH）基因的互补DNA片段，其包含sGnRH十肽，促性腺激素释放激素相关肽和3'非编码序列的编码区和草鱼生长激素基因的3'侧翼序列作为停止序列。本发明的方法操作简单方便，为提供解决转基因鱼遗传和生态安全问题的通用技术平台提供依据。

2. 发明授权

US08472355B2 System, apparatus and methods for broadcasting and transmitting ETWS message 有权
标题翻译：用于广播和传输ETWS消息的系统，设备和方法
公开(公告)号：US08472355B2
公开(公告)日：2013-06-25
申请号：US12976759
申请日：2010-12-22
申请人： Zuoyan Zhu
发明人： Zuoyan Zhu
IPC分类号： H04M11/04
CPC分类号： H04W76/40 , H04W4/90 , H04W76/50
摘要： The present invention relates to an earthquake and tsunami warning system (ETWS) technology, and in particular to a system, apparatus and methods for broadcasting and transmitting an ETWS message. The system for broadcasting and transmitting an ETWS message includes: an ETWS message publishing entity, adapted to publish an ETWS message; a cell broadcast center (CBC), adapted to: encapsulate the ETWS message published by the ETWS message publication entity into a cell broadcast service (CBS) message, and send the CBS message; a mobility management entity (MME), adapted to forward the CBS message sent by the CBC to a radio access network (RAN); and the RAN, adapted to: receive the CBS message forwarded by the MME, and send the received CBS message to a user equipment (UE). The preceding system provides a new technical solution for transmitting an ETWS message, which can guarantee the security of the ETWS message transmission by using the security mechanism of the CBS.
摘要翻译：本发明涉及一种地震和海啸预警系统（ETWS）技术，特别涉及用于广播和发送ETWS消息的系统，设备和方法。用于广播和发送ETWS消息的系统包括：ETWS消息发布实体，适于发布ETWS消息; 小区广播中心（CBC），适于：将由ETWS消息发布实体发布的ETWS消息封装成小区广播服务（CBS）消息，并发送CBS消息; 移动性管理实体（MME），适于将由CBC发送的CBS消息转发到无线电接入网络（RAN）; 和RAN，适于：接收由MME转发的CBS消息，并将接收到的CBS消息发送给用户设备（UE）。上述系统为传输ETWS消息提供了新的技术解决方案，通过使用CBS的安全机制，可以保证ETWS消息传输的安全性。

3. 发明授权

US09642062B2 Method, system and network device for obtaining cell reselection priority 有权
公开(公告)号：US09642062B2
公开(公告)日：2017-05-02
申请号：US12829981
申请日：2010-07-02
申请人： Yinghua Huang , Boyun Xie , Zuoyan Zhu , Mingjiang Xie , Xiaofei Ma , Wen Gao , Xiaoying Xu
发明人： Yinghua Huang , Boyun Xie , Zuoyan Zhu , Mingjiang Xie , Xiaofei Ma , Wen Gao , Xiaoying Xu
IPC分类号： H04W36/34 , H04J11/00 , H04W36/24 , H04W48/16
CPC分类号： H04W36/34 , H04J11/0069 , H04W36/24 , H04W48/16
摘要： A method, a system, and a network device for obtaining cell reselection priority are disclosed. The method includes: The network obtains information for calculating priority; and the network determines a terminal-specific private priority list according to the information, and delivers the determined private priority list specific to the terminal through special signaling; or the terminal obtains all or part of the information for calculating priority from the network, and determines its private priority list according to the obtained information; or both the network and the terminal determine the terminal-specific private priority list according to the information.

4. 发明申请

US20100296504A1 METHOD, SYSTEM AND TERMINAL FOR SCHEDULING SYSTEM INFORMATION 审中-公开
标题翻译：方法，系统和终端用于调度系统信息
公开(公告)号：US20100296504A1
公开(公告)日：2010-11-25
申请号：US12849219
申请日：2010-08-03
申请人： Zuoyan ZHU , Mingjiang XIE , Xiaofei MA
发明人： Zuoyan ZHU , Mingjiang XIE , Xiaofei MA
IPC分类号： H04J3/00
CPC分类号： H04W48/12
摘要： Scheduling System Information (SI) in a radio communication system is disclosed. System Frame Number (SFN) and position of System Information is determined in accordance with the SFN. The SI-x is established, and all SIs on the radio frames are distributed discretely at different times for sending. An SI scheduling system and a UE are also disclosed. The technical solution under the present invention optimizes scheduling of SI, avoids excessive network load caused by sequentially sending of all SIs on different sub-frames, and improves the efficiency of scheduling user data.
摘要翻译：公开了无线电通信系统中的调度系统信息（SI）。系统帧号（SFN）和系统信息的位置根据SFN确定。建立SI-x，无线帧上的所有SI分散在不同的时间发送。还公开了SI调度系统和UE。本发明的技术方案优化了SI的调度，避免了在不同子帧上顺序发送所有SI造成的网络负担过重，提高了用户数据调度的效率。

5. 发明申请

US20100273485A1 METHOD, SYSTEM AND NETWORK DEVICE FOR OBTAINING CELL RESELECTION PRIORITY 有权
标题翻译：方法，用于获取细胞评估优先权的系统和网络设备
公开(公告)号：US20100273485A1
公开(公告)日：2010-10-28
申请号：US12829981
申请日：2010-07-02
申请人： Yinghua HUANG , Boyun XIE , Zuoyan ZHU , Mingjiang XIE , Xiaofei MA , Wen GAO , Xiaoying XU
发明人： Yinghua HUANG , Boyun XIE , Zuoyan ZHU , Mingjiang XIE , Xiaofei MA , Wen GAO , Xiaoying XU
IPC分类号： H04W36/00
CPC分类号： H04W36/34 , H04J11/0069 , H04W36/24 , H04W48/16
摘要： A method, a system, and a network device for obtaining cell reselection priority are disclosed. The method includes: The network obtains information for calculating priority; and the network determines a terminal-specific private priority list according to the information, and delivers the determined private priority list specific to the terminal through special signaling; or the terminal obtains all or part of the information for calculating priority from the network, and determines its private priority list according to the obtained information; or both the network and the terminal determine the terminal-specific private priority list according to the information.
摘要翻译：公开了一种用于获得小区重选优先级的方法，系统和网络设备。该方法包括：网络获取优先级计算信息; 并且网络根据该信息确定终端专用私有优先级列表，并且通过特殊信令传递确定的终端专用优先级列表; 或终端从网络获取用于计算优先级的全部或部分信息，并根据获得的信息确定其私有优先级列表; 或者网络和终端根据该信息确定终端专用私有优先级列表。

6. 发明授权

US08546644B2 Recombinant gene which enhances the ability of fish to tolerate low dissolved oxygen stress and the use thereof 失效
标题翻译：提高鱼容忍低溶解氧应激能力的重组基因及其应用
公开(公告)号：US08546644B2
公开(公告)日：2013-10-01
申请号：US12879931
申请日：2010-09-10
申请人： Wei Hu , Zuoyan Zhu , Hong Ma , Bo Guan , Yuanlei Hu , Zhongping Lin
发明人： Wei Hu , Zuoyan Zhu , Hong Ma , Bo Guan , Yuanlei Hu , Zhongping Lin
IPC分类号： A01K67/027 , C12N15/85 , C12N15/873 , C12N15/90
CPC分类号： C12N15/8509 , A01K2227/40 , A01K2267/02 , C07K14/805 , C12N2830/85
摘要： The invention discloses a recombinant gene which enhances the ability of fish to tolerate low dissolved oxygen (DO) stress and the use thereof. Carp β-actin gene promoter is used as a promoter and Vitreoscilla hemoglobin gene is used as a target gene, so as to construct the recombinant Vitreoscilla hemoglobin gene driven by carp β-actin promoter. The modeling organism zebrafish is used as the research object, and the recombinant gene is microinjected into zygotes of zebrafish. After PCR screening and 156 h low DO stress test, transgenic fish are obtained with a survival rate of 92%, which is significantly different from the survival rate of 65% of the control fish group. The vhb transgenic zebrafish obtain hypoxia tolerance. When the recombinant gene is applied to the economically farmed species, i.e., blunt snout bream (Megalobrama amblycephala) and common carp (Cyprinus carpio L.), it enhances their hypoxia tolerance as well. Such genetically improved breeding technique may be widely used for breeding new excellent farmed species with the hypoxia tolerance.
摘要翻译：本发明公开了一种提高鱼耐受低溶解氧（DO）应力的能力及其用途的重组基因。将鲤鱼β-肌动蛋白基因启动子用作启动子，并使用透明颤菌血红蛋白基因作为靶基因，构建由鲤鱼β-肌动蛋白启动子驱动的重组透明颤菌血红蛋白基因。将造型生物斑马鱼作为研究对象，将重组基因显微注射到斑马鱼的合子中。 PCR筛选和156小时DO胁迫试验后，获得转基因鱼的成活率为92％，与对照鱼组65％的存活率显着不同。 vhb转基因斑马鱼获得缺氧耐受。当将重组基因应用于经济养殖的物种，即钝鼻鲷（Megalobrama amblycephala）和鲤鱼（Cyprinus carpio L.）时，也增强了它们的缺氧耐受性。这种遗传改良育种技术可广泛用于育种具有缺氧耐受性的新优良种植品种。

7. 发明申请

US20110099648A1 RECOMBINANT GENE WHICH ENHANCES THE ABILITY OF FISH TO TOLERATE LOW DISSOLVED OXYGEN STRESS AND THE USE THEREOF 失效
标题翻译：提高鱼的耐受能力，以减少低溶解氧的应力及其使用的重组基因
公开(公告)号：US20110099648A1
公开(公告)日：2011-04-28
申请号：US12879931
申请日：2010-09-10
申请人： WEI HU , ZUOYAN ZHU , HONG MA , BO GUAN , YUANLEI HU , ZHONGPING LIN
发明人： WEI HU , ZUOYAN ZHU , HONG MA , BO GUAN , YUANLEI HU , ZHONGPING LIN
IPC分类号： A01K67/027 , C12N15/63 , C12N15/00
CPC分类号： C12N15/8509 , A01K2227/40 , A01K2267/02 , C07K14/805 , C12N2830/85
摘要： The invention discloses a recombinant gene which enhances the ability of fish to tolerate low dissolved oxygen (DO) stress and the use thereof. Carp β-actin gene promoter is used as a promoter and Vitreoscilla hemoglobin gene is used as a target gene, so as to construct the recombinant Vitreoscilla hemoglobin gene driven by carp β-actin promoter. The modeling organism zebrafish is used as the research object, and the recombinant gene is microinjected into zygotes of zebrafish. After PCR screening and 156 h low DO stress test, transgenic fish are obtained with a survival rate of 92%, which is significantly different from the survival rate of 65% of the control fish group. The vhb transgenic zebrafish obtain hypoxia tolerance. When the recombinant gene is applied to the economically farmed species, i.e., blunt snout bream (Megalobrama amblycephala) and common carp (Cyprinus carpio L.), it enhances their hypoxia tolerance as well. Such genetically improved breeding technique may be widely used for breeding new excellent farmed species with the hypoxia tolerance.
摘要翻译：本发明公开了一种提高鱼耐受低溶解氧（DO）应力的能力及其用途的重组基因。使用鲤鱼β-肌动蛋白基因启动子作为启动子，将透明颤菌血红蛋白基因作为靶基因，构建由鲤鱼和肌动蛋白启动子驱动的重组透明颤菌血红蛋白基因。将造型生物斑马鱼作为研究对象，将重组基因显微注射到斑马鱼的合子中。 PCR筛选和156小时DO胁迫试验后，获得转基因鱼的成活率为92％，与对照鱼组65％的存活率显着不同。 vhb转基因斑马鱼获得缺氧耐受。当将重组基因应用于经济养殖的物种，即钝鼻鲷（Megalobrama amblycephala）和鲤鱼（Cyprinus carpio L.）时，也增强了它们的缺氧耐受性。这种遗传改良育种技术可广泛用于育种具有缺氧耐受性的新优良种植品种。

8. 发明申请

US20110090828A1 SYSTEM, APPARATUS AND METHODS FOR BROADCASTING AND TRANSMITTING ETWS MESSAGE 有权
标题翻译：系统，装置和方法，用于广播和传输ETWS消息
公开(公告)号：US20110090828A1
公开(公告)日：2011-04-21
申请号：US12976759
申请日：2010-12-22
申请人： Zuoyan Zhu
发明人： Zuoyan Zhu
IPC分类号： H04W4/22 , H04W88/04
CPC分类号： H04W76/40 , H04W4/90 , H04W76/50
摘要： The present invention relates to an earthquake and tsunami warning system (ETWS) technology, and in particular to a system, apparatus and methods for broadcasting and transmitting an ETWS message. The system for broadcasting and transmitting an ETWS message includes: an ETWS message publishing entity, adapted to publish an ETWS message; a cell broadcast center (CBC), adapted to: encapsulate the ETWS message published by the ETWS message publication entity into a cell broadcast service (CBS) message, and send the CBS message; a mobility management entity (MME), adapted to forward the CBS message sent by the CBC to a radio access network (RAN); and the RAN, adapted to: receive the CBS message forwarded by the MME, and send the received CBS message to a user equipment (UE). The preceding system provides a new technical solution for transmitting an ETWS message, which can guarantee the security of the ETWS message transmission by using the security mechanism of the CBS.
摘要翻译：本发明涉及一种地震和海啸预警系统（ETWS）技术，特别涉及用于广播和发送ETWS消息的系统，设备和方法。用于广播和发送ETWS消息的系统包括：ETWS消息发布实体，适于发布ETWS消息; 小区广播中心（CBC），适于：将由ETWS消息发布实体发布的ETWS消息封装成小区广播服务（CBS）消息，并发送CBS消息; 移动性管理实体（MME），适于将由CBC发送的CBS消息转发到无线电接入网络（RAN）; 和RAN，适于：接收由MME转发的CBS消息，并将接收到的CBS消息发送给用户设备（UE）。上述系统为传输ETWS消息提供了新的技术解决方案，通过使用CBS的安全机制，可以保证ETWS消息传输的安全性。

9. 发明授权

US07230156B2 Method for preparing a sterile transgenic carp 失效
标题翻译：制备无菌转基因鲤鱼的方法
公开(公告)号：US07230156B2
公开(公告)日：2007-06-12
申请号：US11094286
申请日：2005-03-31
申请人： Wei Hu , Zuoyan Zhu , Yaping Wang , Shuangfei Li
发明人： Wei Hu , Zuoyan Zhu , Yaping Wang , Shuangfei Li
IPC分类号： A01K67/027 , C12N15/00
CPC分类号： C07K14/461 , A01K67/0275 , A01K2217/05 , A01K2227/40 , A01K2267/02
摘要： The present application disclosed a method for preparing a sterile transgenic fish, comprising constructing antisense RNA expression vector of salmon-type gonadotropin-releasing hormone, introducing the recombinant DNA fragment into carp oosperm by microinjection, and screening the sterile transgenic fish by Polymerase Chain Reaction and radioimmunoassay, wherein the expression vector comprising a promoter of carp beta actin (β-actin) gene, a complementary DNA fragment of antisense salmon-type gonadotropin-releasing hormone (sGnRH) gene from carp with 323 bp as a target gene comprising sGnRH decapeptide, the coding region of gonadotropin-releasing hormone associated peptide and 3′ non-coding sequence, and 3′ flanking sequence of grass carp growth hormone gene as a stop sequence. The method of the present invention is easy and convenient for operation which provides a basis for providing a technical platform of general applicability for solving the hereditary and ecological safety problems of transgenic fishes.
摘要翻译：本申请公开了一种制备无菌转基因鱼的方法，包括构建鲑鱼型促性腺激素释放激素的反义RNA表达载体，通过显微注射将重组DNA片段引入鲤鱼胚乳，并通过聚合酶链式反应筛选无菌转基因鱼，放射免疫测定法，其中包含鲤鱼β肌动蛋白（β-肌动蛋白）基因启动子的表达载体，具有323bp作为靶基因的来自鲤鱼的反义鲑鱼型促性腺激素释放激素（sGnRH）基因的互补DNA片段，其包含sGnRH十肽，促性腺激素释放激素相关肽和3'非编码序列的编码区和草鱼生长激素基因的3'侧翼序列作为停止序列。本发明的方法操作简单方便，为提供解决转基因鱼遗传和生态安全问题的通用技术平台提供依据。

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