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1. 发明授权

US08546644B2 Recombinant gene which enhances the ability of fish to tolerate low dissolved oxygen stress and the use thereof 失效
标题翻译：提高鱼容忍低溶解氧应激能力的重组基因及其应用
公开(公告)号：US08546644B2
公开(公告)日：2013-10-01
申请号：US12879931
申请日：2010-09-10
申请人： Wei Hu , Zuoyan Zhu , Hong Ma , Bo Guan , Yuanlei Hu , Zhongping Lin
发明人： Wei Hu , Zuoyan Zhu , Hong Ma , Bo Guan , Yuanlei Hu , Zhongping Lin
IPC分类号： A01K67/027 , C12N15/85 , C12N15/873 , C12N15/90
CPC分类号： C12N15/8509 , A01K2227/40 , A01K2267/02 , C07K14/805 , C12N2830/85
摘要： The invention discloses a recombinant gene which enhances the ability of fish to tolerate low dissolved oxygen (DO) stress and the use thereof. Carp β-actin gene promoter is used as a promoter and Vitreoscilla hemoglobin gene is used as a target gene, so as to construct the recombinant Vitreoscilla hemoglobin gene driven by carp β-actin promoter. The modeling organism zebrafish is used as the research object, and the recombinant gene is microinjected into zygotes of zebrafish. After PCR screening and 156 h low DO stress test, transgenic fish are obtained with a survival rate of 92%, which is significantly different from the survival rate of 65% of the control fish group. The vhb transgenic zebrafish obtain hypoxia tolerance. When the recombinant gene is applied to the economically farmed species, i.e., blunt snout bream (Megalobrama amblycephala) and common carp (Cyprinus carpio L.), it enhances their hypoxia tolerance as well. Such genetically improved breeding technique may be widely used for breeding new excellent farmed species with the hypoxia tolerance.
摘要翻译：本发明公开了一种提高鱼耐受低溶解氧（DO）应力的能力及其用途的重组基因。将鲤鱼β-肌动蛋白基因启动子用作启动子，并使用透明颤菌血红蛋白基因作为靶基因，构建由鲤鱼β-肌动蛋白启动子驱动的重组透明颤菌血红蛋白基因。将造型生物斑马鱼作为研究对象，将重组基因显微注射到斑马鱼的合子中。 PCR筛选和156小时DO胁迫试验后，获得转基因鱼的成活率为92％，与对照鱼组65％的存活率显着不同。 vhb转基因斑马鱼获得缺氧耐受。当将重组基因应用于经济养殖的物种，即钝鼻鲷（Megalobrama amblycephala）和鲤鱼（Cyprinus carpio L.）时，也增强了它们的缺氧耐受性。这种遗传改良育种技术可广泛用于育种具有缺氧耐受性的新优良种植品种。

2. 发明申请

US20110099648A1 RECOMBINANT GENE WHICH ENHANCES THE ABILITY OF FISH TO TOLERATE LOW DISSOLVED OXYGEN STRESS AND THE USE THEREOF 失效
标题翻译：提高鱼的耐受能力，以减少低溶解氧的应力及其使用的重组基因
公开(公告)号：US20110099648A1
公开(公告)日：2011-04-28
申请号：US12879931
申请日：2010-09-10
申请人： WEI HU , ZUOYAN ZHU , HONG MA , BO GUAN , YUANLEI HU , ZHONGPING LIN
发明人： WEI HU , ZUOYAN ZHU , HONG MA , BO GUAN , YUANLEI HU , ZHONGPING LIN
IPC分类号： A01K67/027 , C12N15/63 , C12N15/00
CPC分类号： C12N15/8509 , A01K2227/40 , A01K2267/02 , C07K14/805 , C12N2830/85
摘要： The invention discloses a recombinant gene which enhances the ability of fish to tolerate low dissolved oxygen (DO) stress and the use thereof. Carp β-actin gene promoter is used as a promoter and Vitreoscilla hemoglobin gene is used as a target gene, so as to construct the recombinant Vitreoscilla hemoglobin gene driven by carp β-actin promoter. The modeling organism zebrafish is used as the research object, and the recombinant gene is microinjected into zygotes of zebrafish. After PCR screening and 156 h low DO stress test, transgenic fish are obtained with a survival rate of 92%, which is significantly different from the survival rate of 65% of the control fish group. The vhb transgenic zebrafish obtain hypoxia tolerance. When the recombinant gene is applied to the economically farmed species, i.e., blunt snout bream (Megalobrama amblycephala) and common carp (Cyprinus carpio L.), it enhances their hypoxia tolerance as well. Such genetically improved breeding technique may be widely used for breeding new excellent farmed species with the hypoxia tolerance.
摘要翻译：本发明公开了一种提高鱼耐受低溶解氧（DO）应力的能力及其用途的重组基因。使用鲤鱼β-肌动蛋白基因启动子作为启动子，将透明颤菌血红蛋白基因作为靶基因，构建由鲤鱼和肌动蛋白启动子驱动的重组透明颤菌血红蛋白基因。将造型生物斑马鱼作为研究对象，将重组基因显微注射到斑马鱼的合子中。 PCR筛选和156小时DO胁迫试验后，获得转基因鱼的成活率为92％，与对照鱼组65％的存活率显着不同。 vhb转基因斑马鱼获得缺氧耐受。当将重组基因应用于经济养殖的物种，即钝鼻鲷（Megalobrama amblycephala）和鲤鱼（Cyprinus carpio L.）时，也增强了它们的缺氧耐受性。这种遗传改良育种技术可广泛用于育种具有缺氧耐受性的新优良种植品种。

3. 发明申请

US20110033946A1 DETECTION OF SHORT-CHAIN FATTY ACIDS IN BIOLOGICAL SAMPLES 审中-公开
标题翻译：检测生物样品中短链脂肪酸
公开(公告)号：US20110033946A1
公开(公告)日：2011-02-10
申请号：US12834720
申请日：2010-07-12
申请人： Ronald J. Berenson , Patrick Bobbitt , Zhongping Lin
发明人： Ronald J. Berenson , Patrick Bobbitt , Zhongping Lin
IPC分类号： G01N33/50
CPC分类号： G01N30/88 , G01N2030/8822 , Y10T436/201666
摘要： Described herein is a method of detecting and/or quantifying analytes, such as short-chain fatty acids. Analysis for the presence and/or quantity of the small molecule can be performed on a biological sample from a subject. In some embodiments, a liquid chromatography/mass spectrometry (LC-MS/MS) instrumentation is combined with a solid-phase extraction (SPE). Methods of derivatization can also be incorporated with LC-MS/MS and SPE instrumentation to detect and quantify target analytes. In addition to derivation, methods of reconstituting derivatized molecules can also be incorporated with LC-MS/MS and SPE instrumentation to detect and quantify target analytes.
摘要翻译：本文描述了检测和/或定量分析物的方法，例如短链脂肪酸。可以对来自受试者的生物样品进行小分子的存在和/或数量的分析。在一些实施方案中，将液相色谱/质谱（LC-MS / MS）仪器与固相萃取（SPE）组合。衍生化方法也可以与LC-MS / MS和SPE仪器结合，以检测和定量目标分析物。除了衍生之外，重组衍生化分子的方法也可以与LC-MS / MS和SPE仪器结合，以检测和定量目标分析物。

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