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    • 1. 发明授权
    • System, apparatus and methods for broadcasting and transmitting ETWS message
    • 用于广播和传输ETWS消息的系统,设备和方法
    • US08472355B2
    • 2013-06-25
    • US12976759
    • 2010-12-22
    • Zuoyan Zhu
    • Zuoyan Zhu
    • H04M11/04
    • H04W76/40H04W4/90H04W76/50
    • The present invention relates to an earthquake and tsunami warning system (ETWS) technology, and in particular to a system, apparatus and methods for broadcasting and transmitting an ETWS message. The system for broadcasting and transmitting an ETWS message includes: an ETWS message publishing entity, adapted to publish an ETWS message; a cell broadcast center (CBC), adapted to: encapsulate the ETWS message published by the ETWS message publication entity into a cell broadcast service (CBS) message, and send the CBS message; a mobility management entity (MME), adapted to forward the CBS message sent by the CBC to a radio access network (RAN); and the RAN, adapted to: receive the CBS message forwarded by the MME, and send the received CBS message to a user equipment (UE). The preceding system provides a new technical solution for transmitting an ETWS message, which can guarantee the security of the ETWS message transmission by using the security mechanism of the CBS.
    • 本发明涉及一种地震和海啸预警系统(ETWS)技术,特别涉及用于广播和发送ETWS消息的系统,设备和方法。 用于广播和发送ETWS消息的系统包括:ETWS消息发布实体,适于发布ETWS消息; 小区广播中心(CBC),适于:将由ETWS消息发布实体发布的ETWS消息封装成小区广播服务(CBS)消息,并发送CBS消息; 移动性管理实体(MME),适于将由CBC发送的CBS消息转发到无线电接入网络(RAN); 和RAN,适于:接收由MME转发的CBS消息,并将接收到的CBS消息发送给用户设备(UE)。 上述系统为传输ETWS消息提供了新的技术解决方案,通过使用CBS的安全机制,可以保证ETWS消息传输的安全性。
    • 2. 发明申请
    • Method for preparing a sterile transgenic fish
    • 无菌转基因鱼的制备方法
    • US20050257281A1
    • 2005-11-17
    • US11094286
    • 2005-03-31
    • Wei HuZuoyan ZhuYaping WangShuangfei Li
    • Wei HuZuoyan ZhuYaping WangShuangfei Li
    • A01K61/00A01K67/027C07K14/46
    • C07K14/461A01K67/0275A01K2217/05A01K2227/40A01K2267/02
    • The present application disclosed a method for preparing a sterile transgenic fish, comprising constructing antisense RNA expression vector of salmon-type gonadotropin-releasing hormone, introducing the recombinant DNA fragment into carp oosperm by microinjection, and screening the sterile transgenic fish by Polymerase Chain Reaction and radioimmunoassay, wherein the expression vector comprising a promoter of carp beta actin (β-actin) gene, a complementary DNA fragment of antisense salmon-type gonadotropin-releasing hormone (sGnRH) gene from carp with 323 bp as a target gene comprising sGnRH decapeptide, the coding region of gonadotropin-releasing hormone associated peptide and 3′ non-coding sequence, and 3′ flanking sequence of grass carp growth hormone gene as a stop sequence. The method of the present invention is easy and convenient for operation which provides a basis for providing a technical platform of general applicability for solving the hereditary and ecological safety problems of transgenic fishes.
    • 本申请公开了一种制备无菌转基因鱼的方法,包括构建鲑鱼型促性腺激素释放激素的反义RNA表达载体,通过显微注射将重组DNA片段引入鲤鱼胚乳,并通过聚合酶链式反应筛选无菌转基因鱼, 放射免疫测定法,其中包含鲤鱼β肌动蛋白(β-肌动蛋白)基因启动子的表达载体,具有323bp作为靶基因的来自鲤鱼的反义鲑鱼型促性腺激素释放激素(sGnRH)基因的互补DNA片段,其包含sGnRH十肽, 促性腺激素释放激素相关肽和3'非编码序列的编码区和草鱼生长激素基因的3'侧翼序列作为停止序列。 本发明的方法操作简单方便,为提供解决转基因鱼遗传和生态安全问题的通用技术平台提供依据。
    • 4. 发明授权
    • Recombinant gene which enhances the ability of fish to tolerate low dissolved oxygen stress and the use thereof
    • 提高鱼容忍低溶解氧应激能力的重组基因及其应用
    • US08546644B2
    • 2013-10-01
    • US12879931
    • 2010-09-10
    • Wei HuZuoyan ZhuHong MaBo GuanYuanlei HuZhongping Lin
    • Wei HuZuoyan ZhuHong MaBo GuanYuanlei HuZhongping Lin
    • A01K67/027C12N15/85C12N15/873C12N15/90
    • C12N15/8509A01K2227/40A01K2267/02C07K14/805C12N2830/85
    • The invention discloses a recombinant gene which enhances the ability of fish to tolerate low dissolved oxygen (DO) stress and the use thereof. Carp β-actin gene promoter is used as a promoter and Vitreoscilla hemoglobin gene is used as a target gene, so as to construct the recombinant Vitreoscilla hemoglobin gene driven by carp β-actin promoter. The modeling organism zebrafish is used as the research object, and the recombinant gene is microinjected into zygotes of zebrafish. After PCR screening and 156 h low DO stress test, transgenic fish are obtained with a survival rate of 92%, which is significantly different from the survival rate of 65% of the control fish group. The vhb transgenic zebrafish obtain hypoxia tolerance. When the recombinant gene is applied to the economically farmed species, i.e., blunt snout bream (Megalobrama amblycephala) and common carp (Cyprinus carpio L.), it enhances their hypoxia tolerance as well. Such genetically improved breeding technique may be widely used for breeding new excellent farmed species with the hypoxia tolerance.
    • 本发明公开了一种提高鱼耐受低溶解氧(DO)应力的能力及其用途的重组基因。 将鲤鱼β-肌动蛋白基因启动子用作启动子,并使用透明颤菌血红蛋白基因作为靶基因,构建由鲤鱼β-肌动蛋白启动子驱动的重组透明颤菌血红蛋白基因。 将造型生物斑马鱼作为研究对象,将重组基因显微注射到斑马鱼的合子中。 PCR筛选和156小时DO胁迫试验后,获得转基因鱼的成活率为92%,与对照鱼组65%的存活率显着不同。 vhb转基因斑马鱼获得缺氧耐受。 当将重组基因应用于经济养殖的物种,即钝鼻鲷(Megalobrama amblycephala)和鲤鱼(Cyprinus carpio L.)时,也增强了它们的缺氧耐受性。 这种遗传改良育种技术可广泛用于育种具有缺氧耐受性的新优良种植品种。
    • 5. 发明申请
    • SYSTEM, APPARATUS AND METHODS FOR BROADCASTING AND TRANSMITTING ETWS MESSAGE
    • 系统,装置和方法,用于广播和传输ETWS消息
    • US20110090828A1
    • 2011-04-21
    • US12976759
    • 2010-12-22
    • Zuoyan Zhu
    • Zuoyan Zhu
    • H04W4/22H04W88/04
    • H04W76/40H04W4/90H04W76/50
    • The present invention relates to an earthquake and tsunami warning system (ETWS) technology, and in particular to a system, apparatus and methods for broadcasting and transmitting an ETWS message. The system for broadcasting and transmitting an ETWS message includes: an ETWS message publishing entity, adapted to publish an ETWS message; a cell broadcast center (CBC), adapted to: encapsulate the ETWS message published by the ETWS message publication entity into a cell broadcast service (CBS) message, and send the CBS message; a mobility management entity (MME), adapted to forward the CBS message sent by the CBC to a radio access network (RAN); and the RAN, adapted to: receive the CBS message forwarded by the MME, and send the received CBS message to a user equipment (UE). The preceding system provides a new technical solution for transmitting an ETWS message, which can guarantee the security of the ETWS message transmission by using the security mechanism of the CBS.
    • 本发明涉及一种地震和海啸预警系统(ETWS)技术,特别涉及用于广播和发送ETWS消息的系统,设备和方法。 用于广播和发送ETWS消息的系统包括:ETWS消息发布实体,适于发布ETWS消息; 小区广播中心(CBC),适于:将由ETWS消息发布实体发布的ETWS消息封装成小区广播服务(CBS)消息,并发送CBS消息; 移动性管理实体(MME),适于将由CBC发送的CBS消息转发到无线电接入网络(RAN); 和RAN,适于:接收由MME转发的CBS消息,并将接收到的CBS消息发送给用户设备(UE)。 上述系统为传输ETWS消息提供了新的技术解决方案,通过使用CBS的安全机制,可以保证ETWS消息传输的安全性。
    • 6. 发明授权
    • Method for preparing a sterile transgenic carp
    • 制备无菌转基因鲤鱼的方法
    • US07230156B2
    • 2007-06-12
    • US11094286
    • 2005-03-31
    • Wei HuZuoyan ZhuYaping WangShuangfei Li
    • Wei HuZuoyan ZhuYaping WangShuangfei Li
    • A01K67/027C12N15/00
    • C07K14/461A01K67/0275A01K2217/05A01K2227/40A01K2267/02
    • The present application disclosed a method for preparing a sterile transgenic fish, comprising constructing antisense RNA expression vector of salmon-type gonadotropin-releasing hormone, introducing the recombinant DNA fragment into carp oosperm by microinjection, and screening the sterile transgenic fish by Polymerase Chain Reaction and radioimmunoassay, wherein the expression vector comprising a promoter of carp beta actin (β-actin) gene, a complementary DNA fragment of antisense salmon-type gonadotropin-releasing hormone (sGnRH) gene from carp with 323 bp as a target gene comprising sGnRH decapeptide, the coding region of gonadotropin-releasing hormone associated peptide and 3′ non-coding sequence, and 3′ flanking sequence of grass carp growth hormone gene as a stop sequence. The method of the present invention is easy and convenient for operation which provides a basis for providing a technical platform of general applicability for solving the hereditary and ecological safety problems of transgenic fishes.
    • 本申请公开了一种制备无菌转基因鱼的方法,包括构建鲑鱼型促性腺激素释放激素的反义RNA表达载体,通过显微注射将重组DNA片段引入鲤鱼胚乳,并通过聚合酶链式反应筛选无菌转基因鱼, 放射免疫测定法,其中包含鲤鱼β肌动蛋白(β-肌动蛋白)基因启动子的表达载体,具有323bp作为靶基因的来自鲤鱼的反义鲑鱼型促性腺激素释放激素(sGnRH)基因的互补DNA片段,其包含sGnRH十肽, 促性腺激素释放激素相关肽和3'非编码序列的编码区和草鱼生长激素基因的3'侧翼序列作为停止序列。 本发明的方法操作简单方便,为提供解决转基因鱼遗传和生态安全问题的通用技术平台提供依据。