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1. 发明授权

US09714942B2 Detection method and determination method for detection target 有权
公开(公告)号：US09714942B2
公开(公告)日：2017-07-25
申请号：US12810454
申请日：2008-12-25
申请人： Toshiya Sawai , Eri Oowada , Hirokazu Nagaoka , Satoru Sugita , Toshiya Ueki
发明人： Toshiya Sawai , Eri Oowada , Hirokazu Nagaoka , Satoru Sugita , Toshiya Ueki
IPC分类号： G01N27/72 , G01N33/553 , G01N33/543 , G01N27/00
CPC分类号： G01N33/553 , G01N33/54333 , G01N33/54393
摘要： It is intended to provide a detection method and a determination method for detection target capable of detecting and determining a detection target promptly and simply with high accuracy at low cost. The detection method includes the steps of: mixing a first conjugate 10 in which a first substance containing a stimulus-responsive polymer 11 and a particulate magnetic substance 19 is conjugated to a first antibody 13 against a detection target 50 with a sample; applying a magnetic force after placing the resulting mixture in a condition capable of aggregating the stimulus-responsive polymer 11; measuring a generated magnetic field; and detecting the detection target 50 based on the degree of increase in the magnetic field after applying the magnetic force.

2. 发明授权

US09372187B2 Detection method and determination method for detection target 有权
标题翻译：检测目标的检测方法和测定方法
公开(公告)号：US09372187B2
公开(公告)日：2016-06-21
申请号：US12810038
申请日：2008-12-25
申请人： Toshiya Sawai , Eri Oowada , Yasunobu Sato , Hirokazu Nagaoka , Satoru Sugita
发明人： Toshiya Sawai , Eri Oowada , Yasunobu Sato , Hirokazu Nagaoka , Satoru Sugita
IPC分类号： G01N33/533 , G01N33/543
CPC分类号： G01N33/54333 , G01N33/54306
摘要： A detection/determination kit with which a substance to be detected or the amount thereof can be rapidly, inexpensively, and easily detected or determined; and a detection/determination method. The kit, which is for detecting a substance to be detected (50) contained in a specimen, comprises: a first compound (10) comprising a first substance including a stimulus-responsive polymer (11) and, bonded to the first substance, a first antibody (13) against the substance (50); and a second compound (20) comprising a second substance (21) which is hydrophilic and, bonded thereto, a second antibody (23) against the substance (50). The first antibody (13) and second antibody (23) can simultaneously combine with the substance (50) at different sites in the substance (50).
摘要翻译：可以快速，低成本地且容易地检测或确定待检测物质或其量的检测/测定试剂盒; 和检测/确定方法。用于检测样品中所含检测物质（50）的试剂盒包括：第一化合物（10），其包含包含刺激响应性聚合物（11）的第一物质，并且与第一物质结合，针对物质（50）的第一抗体（13）; 和包含第二物质（21）的第二化合物（20），所述第二物质（21）是亲水性的，并且与第二物质（21）键合到针对物质（50）的第二抗体（23）。第一抗体（13）和第二抗体（23）可以与物质（50）中不同部位的物质（50）同时组合。

3. 发明申请

US20110014713A1 DETECTION METHOD AND DETERMINATION METHOD FOR DETECTION TARGET 有权
标题翻译：用于检测目标的检测方法和确定方法
公开(公告)号：US20110014713A1
公开(公告)日：2011-01-20
申请号：US12810038
申请日：2008-12-25
申请人： Toshiya Sawai , Eri Oowada , Yasunobu Sato , Hirokazu Nagaoka , Satoru Sugita
发明人： Toshiya Sawai , Eri Oowada , Yasunobu Sato , Hirokazu Nagaoka , Satoru Sugita
IPC分类号： G01N33/50
CPC分类号： G01N33/54333 , G01N33/54306
摘要： A detection/determination kit with which a substance to be detected or the amount thereof can be rapidly, inexpensively, and easily detected or determined; and a detection/determination method. The kit, which is for detecting a substance to be detected (50) contained in a specimen, comprises: a first compound (10) comprising a first substance including a stimulus-responsive polymer (11) and, bonded to the first substance, a first antibody (13) against the substance (50); and a second compound (20) comprising a second substance (21) which is hydrophilic and, bonded thereto, a second antibody (23) against the substance (50). The first antibody (13) and second antibody (23) can simultaneously combine with the substance (50) at different sites in the substance (50).
摘要翻译：可以快速，低成本地且容易地检测或确定待检测物质或其量的检测/测定试剂盒; 和检测/确定方法。用于检测样品中所含检测物质（50）的试剂盒包括：第一化合物（10），其包含包含刺激响应性聚合物（11）的第一物质，并且与第一物质结合，针对物质（50）的第一抗体（13）; 和包含第二物质（21）的第二化合物（20），所述第二物质（21）是亲水性的，并且与第二物质（21）键合到针对物质（50）的第二抗体（23）。第一抗体（13）和第二抗体（23）可以与物质（50）中不同部位的物质（50）同时组合。

4. 发明授权

US07517678B2 Method for protein production 失效
标题翻译：蛋白质生产方法
公开(公告)号：US07517678B2
公开(公告)日：2009-04-14
申请号：US11366285
申请日：2006-03-02
申请人： Daisuke Koma , Kuniki Kino , Toshiya Sawai
发明人： Daisuke Koma , Kuniki Kino , Toshiya Sawai
IPC分类号： C12P1/00 , C12P21/06 , C12P21/04 , C12N9/00 , C12N9/10 , C12N9/48
CPC分类号： C12P21/02 , C12N9/1096
摘要： Provided is a novel comprising culturing host cells into which a foreign gene has been introduced for producing a protein in a soluble form encoded by the foreign gene, recombinant cells are cultured in the temperature range lower than or equal to the upper temperature limit for growth of the host cells and higher than 5 degrees below the upper temperature limit for growth of the host cells after induction of expression.
摘要翻译：本发明提供一种新颖的方法，其中培养外源基因导入的宿主细胞以外源基因编码的可溶形式产生蛋白质，将重组细胞在低于或等于生长的上限温度的温度范围内培养宿主细胞和诱导表达后宿主细胞生长上限温度高于5度。

5. 发明授权

US6084058A Composition for liquid crystal aligning film, liquid crystal aligning film, liquid crystal displays and liquid crystal display element 失效
标题翻译：液晶取向膜，液晶取向膜，液晶显示器和液晶显示元件的组成
公开(公告)号：US6084058A
公开(公告)日：2000-07-04
申请号：US142559
申请日：1998-09-11
申请人： Shizuo Murata , Toshiya Sawai , Satoshi Tanioka , Haruo Kato
发明人： Shizuo Murata , Toshiya Sawai , Satoshi Tanioka , Haruo Kato
IPC分类号： C08G73/10 , C09K19/30 , G02F1/1337 , G02F1/13
CPC分类号： C09K19/3001 , C08G73/1042 , G02F1/133723 , Y10T428/1018 , Y10T428/31721
摘要： A composition for a polyimide type liquid crystal aligning film characterized in that a diamino compound constituting polyimide contains one, two or more diamine selected from 4,4'-diaminodiphenyl methane, 3,3'-dimethyl-4,4'-diaminodiphenyl methane, 2,2'-dimethyl-4,4'-diaminodiphenyl methane, 3,3',5,5'-tetramethyl-4,4'-diaminodiphenyl methane and 4,4'-ethylene di-meta-toluidine.
摘要翻译： PCT No.PCT / JP97 / 04763 Sec。 371日期：1998年9月11日 102（e）日期1998年9月11日PCT 1997年12月24日PCT公布。第WO98 / 31725号公报日期：1998年7月23日一种聚酰亚胺型液晶取向膜用组合物，其特征在于，构成聚酰亚胺的二氨基化合物含有选自4,4'-二氨基二苯甲烷，3,3'-二甲基-4,4 二氨基二苯基甲烷，2,2'-二甲基-4,4'-二氨基二苯基甲烷，3,3'，5,5'-四甲基-4,4'-二氨基二苯基甲烷和4,4'-亚乙基二 - 甲苯胺。

6. 发明授权

US5807961A Polyamic acid, polyimide film and liquid crystal aligning film and liquid crystal display element using the formers 失效
标题翻译：聚酰胺酸，聚酰亚胺薄膜和液晶取向膜以及使用前者的液晶显示元件
公开(公告)号：US5807961A
公开(公告)日：1998-09-15
申请号：US860013
申请日：1997-06-20
申请人： Toshiya Sawai , Masaaki Yazawa , Seiji Oikawa , Shizuo Murata , Masaharu Hayakawa , Etsuo Nakagawa
发明人： Toshiya Sawai , Masaaki Yazawa , Seiji Oikawa , Shizuo Murata , Masaharu Hayakawa , Etsuo Nakagawa
IPC分类号： C08G73/10 , C09K19/30 , G02F1/1337
CPC分类号： C09K19/3001 , C08G73/101 , C08G73/1014 , C08G73/1017 , C08G73/1042 , C08G73/106 , G02F1/133723 , Y10T428/31721
摘要： This invention is directed to a polyimide film useful as use applications for electronic materials; a liquid crystal aligning film using the same; a liquid crystal display element provided with the liquid crystal aligning film; and a polyamic acid used as a raw material of the polyimide constituting the polyimide film. The polyimide used for preparing the above polyimide films, consists of a diamine component, the 50% by mol or more based upon the total diamine, of which is a diamine having a core structure having no polar group such as 1,2-bis(4-(4-aminobenzyl)phenyl)ethane, 1,6-bis(4-(4-aminobenzyl)phenyl)hexane, 1,1-bis(4-(4-aminobenzyl)phenyl)heptane, etc., and a tetracarboxylic acid dianhydride, and if necessary, an aminosilicon compound, is used as a liquid crystal aligning film.
摘要翻译： PCT No.PCT / JP96 / 03029 Sec。 371日期：1997年6月20日 102（e）日期1996年6月20日PCT 1996年10月17日PCT公布。第WO97 / 14742号公报日期1997年04月24日本发明涉及可用作电子材料用途的聚酰亚胺膜; 使用其的液晶取向膜; 设置有液晶取向膜的液晶显示元件; 和作为构成聚酰亚胺膜的聚酰亚胺的原料的聚酰胺酸。用于制备上述聚酰亚胺膜的聚酰亚胺由二胺组分组成，基于总二胺的50摩尔％以上，其为具有不具有极性基团的核心结构的二胺，例如1,2-双（ 4-（4-氨基苄基）苯基）乙烷，1,6-双（4-（4-氨基苄基）苯基）己烷，1,1-双（4-（4-氨基苄基）苯基）庚烷等，四羧酸二酐，如果需要，使用氨基硅化合物作为液晶取向膜。

7. 发明授权

US5430195A Diamino compounds and liquid crystal aligning films 失效
公开(公告)号：US5430195A
公开(公告)日：1995-07-04
申请号：US328463
申请日：1994-10-25
申请人： Minoru Nakayama , Toshiya Sawai , Shizuo Murata
发明人： Minoru Nakayama , Toshiya Sawai , Shizuo Murata
IPC分类号： C07C217/90 , C08G73/10 , G02F1/1337 , C07C205/35 , C07C217/14
CPC分类号： C08G73/105 , C07C217/90 , G02F1/133723 , C07C2101/14 , Y10T428/1014 , Y10T428/1027
摘要： The present invention is to provide new diamino compounds useful for raw materials for production of liquid crystal aligning films that have excellent aligning properties and uniform and high pretilt angles over the whole display surface of wide substrates without afterimage phenomenons. The liquid crystal aligning films are constituted by a certain polyimide having as a principal constituent a structure unit represented by the formula; ##STR1##

8. 发明授权

US5414126A Diamino compounds and liquid crystal aligning films 失效
标题翻译：二氨基化合物和液晶取向膜
公开(公告)号：US5414126A
公开(公告)日：1995-05-09
申请号：US141600
申请日：1993-10-27
申请人： Minoru Nakayama , Toshiya Sawai , Shizuo Murata
发明人： Minoru Nakayama , Toshiya Sawai , Shizuo Murata
IPC分类号： C07C217/90 , C08G73/10 , G02F1/1337 , C07C217/14
CPC分类号： C08G73/105 , C07C217/90 , G02F1/133723 , C07C2101/14 , Y10T428/1014 , Y10T428/1027
摘要： The present invention is to provide new diamino compounds useful for raw materials for production of liquid crystal aligning films that have excellent aligning properties and uniform and high pretilt angles over the whole display surface of wide substrates without afterimage phenomenons. The liquid crystal aligning films are constituted by a certain polyimide having as a principal constituent a structure unit represented by the formula; ##STR1##
摘要翻译：本发明提供新的二氨基化合物，其用于生产液晶取向膜的原料，其具有优异的取向性能，在宽的基材的整个显示表面上具有均匀且高的预倾角，而没有残留图像现象。液晶取向膜由具有下式表示的结构单元作为主要成分的某种聚酰亚胺构成; （4）

9. 发明申请

US20100330688A1 DETECTION METHOD AND DETERMINATION METHOD FOR DETECTION TARGET 有权
标题翻译：用于检测目标的检测方法和确定方法
公开(公告)号：US20100330688A1
公开(公告)日：2010-12-30
申请号：US12810454
申请日：2008-12-25
申请人： Toshiya Sawai , Eri Oowada , Hirokazu Nagaoka , Satoru Sugita , Toshiya Ueki
发明人： Toshiya Sawai , Eri Oowada , Hirokazu Nagaoka , Satoru Sugita , Toshiya Ueki
IPC分类号： G01N27/72
CPC分类号： G01N33/553 , G01N33/54333 , G01N33/54393
摘要： It is intended to provide a detection method and a determination method for detection target capable of detecting and determining a detection target promptly and simply with high accuracy at low cost. The detection method includes the steps of: mixing a first conjugate 10 in which a first substance containing a stimulus-responsive polymer 11 and a particulate magnetic substance 19 is conjugated to a first antibody 13 against a detection target 50 with a sample; applying a magnetic force after placing the resulting mixture in a condition capable of aggregating the stimulus-responsive polymer 11; measuring a generated magnetic field; and detecting the detection target 50 based on the degree of increase in the magnetic field after applying the magnetic force.
摘要翻译：旨在提供一种能够以低成本快速且简单地以高精度检测和确定检测目标的检测目标的检测方法和确定方法。检测方法包括以下步骤：将含有刺激响应聚合物11的第一物质和颗粒状磁性物质19的第一共轭物10与第一抗体13相对于检测靶50与样品混合; 在将所得混合物置于能够聚集刺激响应性聚合物11的条件之后施加磁力; 测量产生的磁场; 并且基于施加磁力后的磁场的增加程度来检测检测目标50。

10. 发明申请

US20060199249A1 Method for protein production 失效
标题翻译：蛋白质生产方法
公开(公告)号：US20060199249A1
公开(公告)日：2006-09-07
申请号：US11366285
申请日：2006-03-02
申请人： Daisuke Koma , Kuniki Kino , Toshiya Sawai
发明人： Daisuke Koma , Kuniki Kino , Toshiya Sawai
IPC分类号： C12P21/06 , C12N15/74 , C12N1/21 , C07K14/185
CPC分类号： C12P21/02 , C12N9/1096
摘要： To provide a novel method for obtaining a protein of interest efficiently in a soluble form when the protein is produced by expressing a foreign gene in host cells such as microorganisms etc. In a method for, by culturing host cells into which a foreign gene has been introduced, producing a protein encoded by the foreign gene, after induction of expression, recombinant cells are cultured in the temperature range lower than or equal to the upper temperature limit for growth of the host cells and higher than 5 degrees below the upper temperature limit for growth of the host cells.
摘要翻译：提供了通过在诸如微生物等宿主细胞中表达外源基因而产生蛋白质时，以可溶形式有效获得感兴趣的蛋白质的新方法。在通过培养外源基因已经被培养的宿主细胞的方法中引入，产生由外源基因编码的蛋白质，在诱导表达后，重组细胞在低于或等于宿主细胞生长的上限温度的温度范围内培养，高于上限温度以上5度以上宿主细胞的生长。

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