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1. 发明申请

US20090197303A1 PROCESS FOR PRODUCING DIPEPTIDES 审中-公开
标题翻译：生产皂苷的方法
公开(公告)号：US20090197303A1
公开(公告)日：2009-08-06
申请号：US12097806
申请日：2006-12-27
申请人： Kuniki Kino , Atsushi Noguchi , Yuji Nakazawa , Makoto Yagasaki
发明人： Kuniki Kino , Atsushi Noguchi , Yuji Nakazawa , Makoto Yagasaki
IPC分类号： C12P21/02 , C07K16/18 , C12N15/11 , C12N15/00 , C12N1/21
CPC分类号： C12P21/02 , C12N9/93
摘要： The present invention provides: a protein having dipeptide-synthesizing activity; DNA encoding the protein; a recombinant DNA comprising the DNA; a transformant transformed with the recombinant DNA; a process for producing the protein having dipeptide-synthesizing activity using the transformant or the like; a process for producing a dipeptide using the protein having dipeptide-synthesizing activity; and a process for producing a dipeptide using, as an enzyme source, a culture of a transformant or a microorganism which produces the protein having dipeptide-synthesizing activity or the like.
摘要翻译：本发明提供：具有二肽合成活性的蛋白质; 编码蛋白质的DNA; 包含DNA的重组DNA; 用重组DNA转化的转化体; 使用转化体等制备具有二肽合成活性的蛋白质的方法; 使用具有二肽合成活性的蛋白质生产二肽的方法; 使用产生具有二肽合成活性的蛋白质的转化体或微生物的培养物作为酶源来生产二肽的方法。

2. 发明申请

US20070218525A1 Process for Producing Dipeptides 审中-公开
标题翻译：生产二肽的方法
公开(公告)号：US20070218525A1
公开(公告)日：2007-09-20
申请号：US11547491
申请日：2005-03-04
申请人： Kuniki Kino , Masaru Sato
发明人： Kuniki Kino , Masaru Sato
IPC分类号： C12P1/04
CPC分类号： C07K5/06 , C12N9/93 , C12P21/02
摘要： The present invention provides a process for producing a dipeptide other than D-alanyl-D-alanine and D-alanyl-D-serine using, as an enzyme source, a culture of a microorganism having the ability to produce a protein having D-alanine-D-alanine ligase activity or a treated matter of the culture, and a process for producing a dipeptide comprising D-amino acid using, as enzyme sources, a culture of a microorganism having the ability to produce the D-amino acid or a treated matter of the culture and a culture of a microorganism having the ability to produce a protein having D-alanine-D-alanine ligase activity or a treated matter of the culture.
摘要翻译：本发明提供了使用D-丙氨酸-D-丙氨酸和D-丙氨酰-D-丝氨酸以外的二肽的制造方法，其使用具有产生具有D-丙氨酸的蛋白质的能力的微生物的培养物作为酶源 D-丙氨酸连接酶活性或培养物的处理物质，以及使用具有生产D-氨基酸能力的微生物的培养物或经处理的D-氨基酸的生产二氨基酸的方法，所述二肽包含D-氨基酸，作为酶源，培养物质和具有产生具有D-丙氨酸-D-丙氨酸连接酶活性的蛋白质的能力的微生物的培养物或培养物的处理物质。

3. 发明授权

US4729952A Process for producing L-glutamic acid by fermentation 失效
标题翻译：通过发酵生产L-谷氨酸的方法
公开(公告)号：US4729952A
公开(公告)日：1988-03-08
申请号：US582883
申请日：1984-02-23
申请人： Kiyoji Hattori , Yukinobu Kotani , Kuniki Kino
发明人： Kiyoji Hattori , Yukinobu Kotani , Kuniki Kino
IPC分类号： C12P13/14 , C12N1/20 , C12R1/13 , C12R1/15
CPC分类号： C12R1/14 , C12P13/14 , C12R1/15 , Y10S435/84 , Y10S435/843
摘要： A process is disclosed for producing L-glutamic acid, the process involves culturing a microorganism belonging to the genus Corynebacterium or Brevibacterium and having both an ability to produce L-glutamic acid and a resistance to .alpha.-naphthoquinoline, an antibiotic inhibiting energy metabolism or a precursor for ubiquinone biosynthesis in a nutrient medium until L-glutamic acid is accumulated in the resulting culture liquor, and recovering the L-glutamic acid therefrom.

4. 发明授权

US07939294B2 DNA encoding proteins having dipeptide-synthesizing activity and methods of using the same 有权
标题翻译：编码具有二肽合成活性的蛋白质的DNA及其使用方法
公开(公告)号：US07939294B2
公开(公告)日：2011-05-10
申请号：US12442472
申请日：2007-09-25
申请人： Kuniki Kino , Youichi Kotanaka , Makoto Yagasaki
发明人： Kuniki Kino , Youichi Kotanaka , Makoto Yagasaki
IPC分类号： C12P21/06
CPC分类号： C12N9/10 , C12P21/02
摘要： The present invention provides a protein having dipeptide-synthesizing activity, a DNA encoding the protein, a recombinant DNA containing the DNA, a transformant obtained via transformation with the recombinant DNA, a process for producing a protein having dipeptide-synthesizing activity using the transformant, a process for producing a dipeptide using a protein having dipeptide-synthesizing activity, and a process for producing a dipeptide using a culture or the like of a transformant or a microorganism that produces a protein having dipeptide-synthesizing activity as an enzyme source.
摘要翻译：本发明提供了具有二肽合成活性的蛋白质，编码该蛋白质的DNA，含有该DNA的重组DNA，通过用该重组DNA转化得到的转化体，使用该转化体产生具有二肽合成活性的蛋白质的方法，使用具有二肽合成活性的蛋白质生产二肽的方法，以及使用产生具有二肽合成活性的蛋白质作为酶源的转化体或微生物的培养物等制备二肽的方法。

5. 发明授权

US07067289B1 Method for producing histidine by fermentation with E. coli 有权
标题翻译：用大肠杆菌发酵生成组氨酸的方法
公开(公告)号：US07067289B1
公开(公告)日：2006-06-27
申请号：US09665617
申请日：2000-09-19
申请人： Kuniki Kino , Tetsuya Abe
发明人： Kuniki Kino , Tetsuya Abe
IPC分类号： C12P13/24
CPC分类号： C12P13/04 , C12P13/24 , C12R1/19
摘要： The present invention provides a method for producing an amino acid selected from the group consisting of L-alanine, L-valine, L-leucine, L-isoleucine, L-methionine, L-phenylalanine, L-proline, glycine, L-serine, L-threonine, L-cysteine, L-tyrosine, L-asparagine, L-glutamine, L-lysine, L-histidine, L-arginine, L-aspartic acid and L-glutamic acid and useful as medicament, chemical agent, food material and feed additive at high industrial efficiency, the method comprising culturing a microorganism having an ability to produce the amino acid and having resistance to an aminoquinoline derivative in a medium, producing and accumulating the amino acid in the present invention in the culture, and recovering the amino acid from the culture.
摘要翻译：本发明提供了选自L-丙氨酸，L-缬氨酸，L-亮氨酸，L-异亮氨酸，L-甲硫氨酸，L-苯丙氨酸，L-脯氨酸，甘氨酸，L-丝氨酸的氨基酸的制备方法，L-苏氨酸，L-半胱氨酸，L-酪氨酸，L-天冬酰胺，L-谷氨酰胺，L-赖氨酸，L-组氨酸，L-精氨酸，L-天冬氨酸和L-谷氨酸，可用作药物，食品原料和饲料添加剂，该方法包括在培养基中培养具有产生氨基酸并具有氨基喹啉衍生物的能力的微生物，在培养物中产生和积累本发明中的氨基酸，以及从培养物中回收氨基酸。

6. 发明授权

US5919670A Process for producing L-amino acids by fermentation 失效
公开(公告)号：US5919670A
公开(公告)日：1999-07-06
申请号：US99036
申请日：1998-06-18
申请人： Kazuyuki Okamoto , Masato Ikeda , Kuniki Kino
发明人： Kazuyuki Okamoto , Masato Ikeda , Kuniki Kino
IPC分类号： C12N1/20 , C12P13/04 , C12P13/08 , C12R1/19
CPC分类号： C12P13/08 , C12P13/04 , Y10S435/849
摘要： The present invention provides a process for producing an L-amino acid which comprises culturing in a nutrient medium a microorganism which is capable of producing the L-amino acid and which can not grow in a synthetic medium containing said L-amino acid as the sole nitrogen source in an amount of 5 mg/ml or below, allowing the L-amino acid to accumulate in the culture, and recovering the L-amino acid from the culture.

7. 发明授权

US5916782A Process for producing aspartase and process for producing L-aspartic acid 失效
标题翻译：生产天冬氨酸的方法和生产L-天冬氨酸的方法
公开(公告)号：US5916782A
公开(公告)日：1999-06-29
申请号：US822002
申请日：1997-03-24
申请人： Junichi Takano , Kuniki Kino
发明人： Junichi Takano , Kuniki Kino
IPC分类号： C12N9/88 , C12P13/20 , C12P21/00
CPC分类号： C12P13/20 , C12N9/88
摘要： Disclosed are a process for producing aspartase which comprises culturing a microorganism belonging to the genus Escherichia being capable of producing aspartase in a medium until aspartase is produced and accumulated in the culture, and recovering aspartase therefrom, wherein dissolved oxygen concentration of the medium is in the range of 0 to 1 ppm on the stage that microbial growth is middle and/or late logarithmic growth phase; and a process for producing L-aspartic acid which comprises converting fumaric acid and ammonia into L-aspartic acid in an aqueous medium in the presence of an enzyme source, wherein said enzyme source is a culture produced in accordance with said aspartase-producing process, cells isolated from the culture, or processed cells thereof.
摘要翻译：公开了一种生产天冬氨酸酶的方法，其包括在培养基中培养属于埃希氏杆菌属的能够产生天冬氨酸酶的微生物，直到天冬氨酸酶产生并累积在培养物中，并从其中回收天冬氨酸酶，其中培养基的溶解氧浓度在在微生物生长是中间和/或后期对数生长期的阶段，0〜1ppm的范围; 以及L-天冬氨酸的制造方法，其特征在于，在酶源的存在下，在水性介质中将富马酸和氨转化为L-天冬氨酸，其中，所述酶源为根据所述天冬氨酰酶生产方法生产的培养物，从培养物中分离的细胞，或其加工细胞。

8. 发明授权

US5484716A Process for producing L-tryptophan by a Corynebacterium strain having decreased phosphoenolpyruvate carboxylase activity 失效
标题翻译：通过具有降低的磷酸烯醇丙酮酸羧化酶活性的棒状杆菌菌株产生L-色氨酸的方法
公开(公告)号：US5484716A
公开(公告)日：1996-01-16
申请号：US130995
申请日：1993-10-04
申请人： Ryoichi Katsumata , Kuniki Kino
发明人： Ryoichi Katsumata , Kuniki Kino
IPC分类号： C12P13/22 , C12R1/13 , C12R1/15
CPC分类号： C12P13/22 , Y10S435/84 , Y10S435/843
摘要： The invention relates to a bacterial process for producing L-tryptophan, L-tyrosine or L-phenylalanine. The process utilizes a coryneform glutamic acid-producing bacterium being capable of producing L-tryptophan, L-tyrosine or L-phenylalanine and also decreased or lacked in phosphoenolpyruvate carboxylase activity. The mutant strain is then cultured in order to accumulate the amino acid in a medium and the amino acid is recovered therefrom.
摘要翻译：本发明涉及生产L-色氨酸，L-酪氨酸或L-苯丙氨酸的细菌方法。该方法利用产生L-色氨酸，L-酪氨酸或L-苯丙氨酸的棒状谷氨酸生产细菌，并且还降低或缺乏磷酸烯醇丙酮酸羧化酶活性。然后培养突变菌株以将氨基酸在培养基中积累，并从中回收氨基酸。

9. 发明授权

US4742007A Process for the preparation of L-tryptophan 失效
标题翻译： L-色氨酸的制备方法
公开(公告)号：US4742007A
公开(公告)日：1988-05-03
申请号：US702560
申请日：1985-02-15
申请人： Kuniki Kino , Toshihide Nakanishi , Masahiro Sugimoto
发明人： Kuniki Kino , Toshihide Nakanishi , Masahiro Sugimoto
IPC分类号： C12P13/22 , C12R1/15 , C12N1/20
CPC分类号： C12R1/15 , C12P13/227 , Y10S435/843
摘要： L-tryptophan can be prepared in good yield by a fermentation process which comprises culturing a novel L-tryptophan-producing microorganism of the genus Corynebacterium, which is resistant to at least one member selected from glyphosate [N-phosphonomethyl glycine], paraquat [1,1'-dimethyl-4,4'-bispyridinium] and derivatives thereof, and recovering L-tryptophan from the culture broth.
摘要翻译：可以通过发酵方法以良好的产率制备L-色氨酸，其包括培养棒状杆菌属的新型L-色氨酸生产微生物，其对选自草甘膦[N-膦酰基甲基甘氨酸]，百草枯[1 ，1'-二甲基-4,4'-二吡啶鎓]及其衍生物，并从培养液中回收L-色氨酸。

10. 发明授权

US09081199B2 Process for the production of dipeptides by a dipeptide-synthesizing enzyme 有权
标题翻译：通过二肽合成酶生产二肽的方法
公开(公告)号：US09081199B2
公开(公告)日：2015-07-14
申请号：US11817905
申请日：2006-03-17
申请人： Kuniki Kino , Yuji Nakazawa , Makoto Yagasaki
发明人： Kuniki Kino , Yuji Nakazawa , Makoto Yagasaki
IPC分类号： C12P21/00 , G02B21/26 , C12N9/00 , G02B21/36
CPC分类号： G02B21/26 , C12N9/93 , G02B21/362
摘要： The present invention provides: a protein having dipeptide-synthesizing activity; DNA encoding the protein; a recombinant DNA comprising the DNA; a transformant transformed with the recombinant DNA; a process for producing the protein having dipeptide-synthesizing activity using the transformant or the like; a process for producing a dipeptide using the protein having dipeptide-synthesizing activity; and a process for producing a dipeptide using, as an enzyme source, a culture of a transformant or a microorganism which produces the protein having dipeptide-synthesizing activity or the like.
摘要翻译：本发明提供：具有二肽合成活性的蛋白质; 编码蛋白质的DNA; 包含DNA的重组DNA; 用重组DNA转化的转化体; 使用转化体等制备具有二肽合成活性的蛋白质的方法; 使用具有二肽合成活性的蛋白质生产二肽的方法; 使用产生具有二肽合成活性的蛋白质的转化体或微生物的培养物作为酶源来生产二肽的方法。

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