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1. 发明申请

US20090203091A1 MUTANTS FOR THE PREPARATION OF D-AMINO ACIDS 审中-公开
标题翻译：用于制备D-氨基酸的突变体
公开(公告)号：US20090203091A1
公开(公告)日：2009-08-13
申请号：US12357218
申请日：2009-01-21
申请人： Oliver MAY , Stefan Buchholz , Michael Schwarm , Karlheinz Drauz , Robert J. Turner , Ian Fotheringham
发明人： Oliver MAY , Stefan Buchholz , Michael Schwarm , Karlheinz Drauz , Robert J. Turner , Ian Fotheringham
IPC分类号： C12P13/04 , C12P13/24 , C12P13/22 , C12N1/21
CPC分类号： C12R1/19 , C12N9/0024 , C12N9/86 , C12N9/90 , C12P13/04 , C12P41/009
摘要： The present invention relates to specific Escherichia. coli mutants which can be used for the synthesis of D-amino acids, and to such a process. The mutants are distinguished by deficiencies in particular enzymes which break down D-amino acids and include those which produce D-amino acids via the carbamoylase/hydantoinase route.
摘要翻译：本发明涉及具体的埃希氏杆菌。可用于合成D-氨基酸的大肠杆菌突变体，以及这种方法。突变体的特征在于分解D-氨基酸的特定酶的缺陷，并且包括通过氨甲酰基酶/乙内酰脲酶途径产生D-氨基酸的那些。

2. 发明申请

US20080216181A1 Novel Alcohol Dehydrogenases 有权
标题翻译：新型酒精脱氢酶
公开(公告)号：US20080216181A1
公开(公告)日：2008-09-04
申请号：US10593119
申请日：2005-03-10
申请人： Renate Schulze , Patrick Lorenz , Jurgen Eck , Oliver May , Harald Groger , Harald Trauthwein
发明人： Renate Schulze , Patrick Lorenz , Jurgen Eck , Oliver May , Harald Groger , Harald Trauthwein
IPC分类号： A01K67/027 , C12N9/02 , C12N15/11 , C12N15/00 , C12N5/06
CPC分类号： C12P7/02 , C12N9/0006 , C12Y101/01002
摘要： The invention relates to novel polypeptides which have the biological activity of an NAD- or NADP-dependent alcohol dehydrogenase. The invention furthermore relates to nucleic acids encoding said polypeptides, to non-human hosts or host cells and to reaction systems which may be used for preparing desired products. The polypeptides of the invention are preferably used in the preparation, starting from aldehydes or ketones, of primary and enantiomerically pure secondary alcohol's which may serve as intermediates for medicaments. Alternatively, the polypeptides of the invention may also be employed in the reverse reaction, i.e. the oxidation of alcohol's with the formation of aldehydes or ketones.
摘要翻译：本发明涉及具有NAD-或NADP依赖性醇脱氢酶的生物活性的新型多肽。本发明还涉及编码所述多肽，非人宿主或宿主细胞的核酸以及可用于制备所需产物的反应体系。本发明的多肽优选用于从醛或酮开始制备可以作为药物中间体的初级和对映体纯的仲醇。或者，也可以将本发明的多肽用于反向反应，即醇与醛或酮的形成的氧化。

3. 发明授权

US07247465B2 Screening process for hydantoin racemases 有权
标题翻译：乙内酰脲消旋体的筛选过程
公开(公告)号：US07247465B2
公开(公告)日：2007-07-24
申请号：US10559434
申请日：2004-05-15
申请人： Oliver May , Karlheinz Drauz , Stefan Buchholz
发明人： Oliver May , Karlheinz Drauz , Stefan Buchholz
IPC分类号： C12N9/88 , C12P1/00 , C12P13/04
CPC分类号： G01N33/573 , C12N9/90 , C12N11/16 , C12P13/04 , C12P41/009
摘要： The present invention relates to a screening process for hydantoin racemases and to novel hydantoin racemases, to the nucleic acid sequences coding therefor and to a proces for mutagenesis.Hydantoin racemases are of interest in connection with the production of enantiomerically enriched amino acids from racemic hydantoins.
摘要翻译：本发明涉及乙内酰脲消旋酶和新型乙内酰脲消旋酶的筛选方法，编码该核酸序列的核酸序列以及用于诱变的过程。海参蛋白消旋酶与从外消旋乙内酰脲生产对映体富集的氨基酸有关。

4. 发明申请

US20070128689A1 Reca-negative and rhab-negative microorganism 审中-公开
标题翻译： Reca阴性和Rhab阴性微生物
公开(公告)号：US20070128689A1
公开(公告)日：2007-06-07
申请号：US10492939
申请日：2002-11-11
申请人： Oliver May , Klaus Liebeton , Jurgen Eck
发明人： Oliver May , Klaus Liebeton , Jurgen Eck
IPC分类号： C12P21/06 , C12N9/16 , C12N15/74 , C07H21/04 , C12N1/21
CPC分类号： C12R1/19 , C12N15/68 , C12N15/70 , C12P13/04 , C12P21/02 , C12P41/006
摘要： The present invention describes new microorganisms specifically for use as expression systems for rhamnose-in-ducible promoters. Due to the combination of rhamnose and recombination deficiency artificially created in these organisms, ex-pression systems which require less of the expensive rhamnose for induction and therefore can be induced more inexpensively, and have a higher genetic stability are obtained.
摘要翻译：本发明描述了专门用作用于鼠李糖苷可启动子的表达系统的新微生物。由于在这些生物体中人造产生的鼠李糖和重组缺陷的组合，需要较少昂贵的鼠李糖用于诱导并因此可以更廉价地诱导并且具有较高遗传稳定性的预压系统。

5. 发明授权

US06524837B1 Hydantoinase variants with improved properties and their use for the production of amino acids 有权
标题翻译：具有改进性质的乙内酰脲酶变体及其用于生产氨基酸的用途
公开(公告)号：US06524837B1
公开(公告)日：2003-02-25
申请号：US09497585
申请日：2000-02-03
申请人： Frances H. Arnold , Oliver May , Karlheinz Drauz , Andreas Bommarius
发明人： Frances H. Arnold , Oliver May , Karlheinz Drauz , Andreas Bommarius
IPC分类号： C12N986
CPC分类号： C12P13/12 , C07H21/04 , C12N9/86 , C12P13/04 , C12P13/06 , C12P13/08 , C12P41/009
摘要： Hydantoinase enzymes which are mutants of a previously isolated hydantoinase having the amino acid SEQ. ID. NO. 2. The mutants include amino acid substitutions at positions 95, 154, 180, 251 and/or 255 of the wild type hydantoinase (SEQ. ID. NO. 2). The mutant hydantoinases, like the parent hydantoinase, are used in the production of optically pure amino acids.
摘要翻译：乙内酰脲酶，其是具有氨基酸SEQ ID NO：1的先前分离的乙内酰脲酶的突变体。 ID。没有。突变体包括野生型乙内酰脲酶（SEQ ID NO：2）的95,54,180,251和/或255位的氨基酸取代。突变的乙内酰脲酶，如亲水乙内酰脲酶，用于生产光学纯氨基酸。

6. 发明申请

US20110177553A1 PREPARATION OF AN ESTERASE 有权
标题翻译：蛋白酶的制备
公开(公告)号：US20110177553A1
公开(公告)日：2011-07-21
申请号：US12667414
申请日：2008-07-04
申请人： Martin Kietzmann , Helmut Schwab , Harald Pichler , Mirela Ivancic , Oliver May , Rudolf Gijsbertus Marie Luiten
发明人： Martin Kietzmann , Helmut Schwab , Harald Pichler , Mirela Ivancic , Oliver May , Rudolf Gijsbertus Marie Luiten
IPC分类号： C12P21/02 , C12N1/21 , C12N15/55 , C12N15/70 , C12P7/40
CPC分类号： C12N9/18 , C12N15/70 , C12Y301/01001
摘要： The present invention relates to a recombinant E. coli strain characterized in that the organisms contains a gene coding for a protein having esterase activity. Such E. coli is suitable for the preparation of a protein with esterase activity, whereby the expression takes place without coexpression of GroEL and/or GroES from a plasmid. The expression of glutathione reductase and/or thioredoxin reductase may be abolished by mutation. Furthermore, the ability of the organism to grow under oxygen-rich conditions has been restored by mutation. The expression of the gene of the protein with esterase activity is unaccompanied by the expression of an additional gene encoding a heat shock chaperone protein.
摘要翻译：本发明涉及重组大肠杆菌菌株，其特征在于生物体含有编码具有酯酶活性的蛋白质的基因。这种大肠杆菌适用于制备具有酯酶活性的蛋白质，从而在不与GroEL和/或GroES共同表达质粒的情况下进行表达。谷胱甘肽还原酶和/或硫氧还蛋白还原酶的表达可以通过突变来消除。此外，生物体在富氧条件下生长的能力已经通过突变恢复。具有酯酶活性的蛋白质基因的表达与编码热休克分子伴侣蛋白的另外的基因的表达无关。

7. 发明申请

US20060210989A1 Screening process for hydantoin racemases 有权
标题翻译：乙内酰脲消旋体的筛选过程
公开(公告)号：US20060210989A1
公开(公告)日：2006-09-21
申请号：US10559434
申请日：2004-05-15
申请人： Oliver May , Karlheinz Drauz , Stefan Buchholz
发明人： Oliver May , Karlheinz Drauz , Stefan Buchholz
IPC分类号： C12Q1/68 , C12Q1/48 , C07H21/04 , C12P13/04 , C12N9/90
CPC分类号： G01N33/573 , C12N9/90 , C12N11/16 , C12P13/04 , C12P41/009
摘要： The present invention relates to a screening process for hydantoin racemases and to novel hydantoin racemases, to the nucleic acid sequences coding therefor and to a proces for mutagenesis. Hydantoin racemases are of interest in connection with the production of enantiomerically enriched amino acids from racemic hydantoins.
摘要翻译：本发明涉及乙内酰脲消旋酶和新型乙内酰脲消旋酶的筛选方法，编码该核酸序列的核酸序列以及用于诱变的过程。海参蛋白消旋酶与从外消旋乙内酰脲生产对映体富集的氨基酸有关。

8. 发明授权

US06825014B2 Process for the preparation of allysine acetal 失效
标题翻译：制备烯丙基缩醛的方法
公开(公告)号：US06825014B2
公开(公告)日：2004-11-30
申请号：US09916501
申请日：2001-07-30
申请人： Hans-Peter Krimmer , Oliver May , Ingo Klement , Karlheinz Drauz , Dietmar Reichert
发明人： Hans-Peter Krimmer , Oliver May , Ingo Klement , Karlheinz Drauz , Dietmar Reichert
IPC分类号： C12P1304
CPC分类号： C12P13/04
摘要： The present invention relates to the preparation of compounds of formula (I): from the corresponding hydantoins by means of an enzymatic process. The L-compound is preferably formed.
摘要翻译：本发明涉及式（I）化合物的制备：通过酶法由相应的乙内酰脲制备。优选形成L-化合物。

9. 发明授权

US07824899B2 Preparation of enantiomerically enriched amines and amides by enzymatic resolution using a sulfonylacetic acid ester as acyl donor 失效
标题翻译：使用磺酰基乙酸酯作为酰基供体通过酶拆分制备对映体富集的胺和酰胺
公开(公告)号：US07824899B2
公开(公告)日：2010-11-02
申请号：US11766164
申请日：2007-06-21
申请人： Harald Groeger , Oliver May , Kai Rossen , Karlheinz Drauz
发明人： Harald Groeger , Oliver May , Kai Rossen , Karlheinz Drauz
IPC分类号： C12P41/00
CPC分类号： C12P11/00 , C12P13/001 , C12P13/02 , C12P41/007
摘要： A process for the preparation of an enantiomerically enriched amine, is performed by a) cleaving a racemic mixture of a reaction product of i) a chiral amine and ii) an acyl donor, in the presence of a hydrolase, to obtain a mixture of an enantiomerically enriched amide and an enantiomerically enriched amine; or b) reacting an amine with an acyl donor, in the presence of a hydrolase, to obtain a mixture of an enantiomerically enriched amide and an enantiomerically enriched amine; and c) separating the enantiomerically enriched amide from the enantiomerically enriched amine, wherein the acyl donor is a sulphonylacetic acid ester. The process (1) leads to high enantioselectivities, and (2) high reactivities, (3) is based on an acyl donor accessible in a simple manner, (4) is suitable for a large number of very different substrates and/or (5) is suitable for carrying out at high substrate concentrations.
摘要翻译：通过a）在水解酶存在下，将i）手性胺和ii）酰基供体的反应产物的外消旋混合物切割，制备对映体富集的胺的方法，得到对映异构体富集的酰胺和对映体富集的胺; 或b）在水解酶存在下使胺与酰基供体反应，得到对映体富集的酰胺和对映体富集的胺的混合物; 和c）从对映异构体富集的胺分离对映体富集的酰胺，其中所述酰基供体是磺酰基乙酸酯。方法（1）导致高对映选择性，和（2）高反应性，（3）基于可以简单方式获得的酰基供体，（4）适用于大量非常不同的底物和/或（5 ）适用于在高底物浓度下进行。

10. 发明申请

US20090258405A1 Preparation of optically active alcohols with whole-cell catalysts 审中-公开
标题翻译：用全细胞催化剂制备光学活性醇
公开(公告)号：US20090258405A1
公开(公告)日：2009-10-15
申请号：US11629407
申请日：2005-06-09
申请人： Harald Groeger , Oliver May , Claudia Rollmann , Francoise Chamouleau , Nicolas Orologas , Karlheinz Drauz
发明人： Harald Groeger , Oliver May , Claudia Rollmann , Francoise Chamouleau , Nicolas Orologas , Karlheinz Drauz
IPC分类号： C12P7/02
CPC分类号： C12P7/02 , C12P7/22
摘要： The present invention relates to a process for the preparation of optically active alcohols from ketones with the aid of whole-cell catalysts comprising an alcohol dehydrogenase and also an enzyme capable of cofactor regeneration, a substrate concentration of at least 500 mM of ketone being provided for the conversion and the conversion being carried out without the addition of an “external” cofactor.
摘要翻译：本发明涉及一种借助于包含醇脱氢酶的全细胞催化剂以及能辅助因子再生的酶制备光学活性醇的方法，提供至少500mM酮的底物浓度用于在不添加“外部”辅因子的情况下进行转换和转换。

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