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1. 发明授权

US08921080B2 Pig liver esterases 有权
标题翻译：猪肝酯酶
公开(公告)号：US08921080B2
公开(公告)日：2014-12-30
申请号：US13265178
申请日：2010-04-26
申请人： Martin Kietzmann , Harald Pichler , Helmut Schwab , Amin El-Heliebi , Christine Winkler , Andreas Braun
发明人： Martin Kietzmann , Harald Pichler , Helmut Schwab , Amin El-Heliebi , Christine Winkler , Andreas Braun
IPC分类号： C12N9/18
CPC分类号： C12N9/18
摘要： The invention relates to an isolated polypeptide having esterase activity comprising an amino acid sequence shown in any one of SEQ ID NO's 2, 4, 6, 8, 10, 12 or 14 or a homologue thereof, comprising an amino acid substitution or deletion of one or more amino acids as shown in said SEQ ID NO's and resulting in a mutant polypeptide having an increased concentration of the fraction of the mutant polypeptide being present as an active and soluble protein in cleared lysate of the mutant polypeptide expressed in E. coli relative to the concentration of the fraction of the polypeptide without the mutation being present as an active and soluble protein in cleared lysate of the polypeptide without the one or more deletion or substitution expressed in E. coli under the same conditions. The invention also relates to nucleic acid encoding the polypeptides according to the invention, and the use of the polypeptides.
摘要翻译：本发明涉及具有酯酶活性的分离多肽，其包含SEQ ID NO：2,4,6,8,10,12或14中任一项所示的氨基酸序列或其同源物，其包含氨基酸取代或缺失的一个或更多的氨基酸，如所述SEQ ID NO所示，并且产生突变体多肽的浓度增加，所述突变体多肽作为活性和可溶性蛋白质存在于大肠杆菌中表达的突变多肽的澄清裂解物中，相对于不存在突变的多肽的级分的浓度作为活性和可溶性蛋白质存在于多肽的澄清裂解物中，而在相同条件下在大肠杆菌中表达的一个或多个缺失或取代。本发明还涉及编码根据本发明的多肽的核酸和多肽的用途。

2. 发明授权

US08642312B2 Polypeptide having esterase activity and recombinant esterase and use thereof 有权
标题翻译：具有酯酶活性的多肽和重组酯酶及其用途
公开(公告)号：US08642312B2
公开(公告)日：2014-02-04
申请号：US13417734
申请日：2012-03-12
申请人： Gerhard Steinbauer , Micheal Stanek , Peter Pojarliev , Wolfgang Skranc , Helmut Schwab , Joannes Kierkels , Marcel Wubbolts , Harald Pichler , Manuela Hermann , Christoph Zenzmaier
发明人： Gerhard Steinbauer , Micheal Stanek , Peter Pojarliev , Wolfgang Skranc , Helmut Schwab , Joannes Kierkels , Marcel Wubbolts , Harald Pichler , Manuela Hermann , Christoph Zenzmaier
IPC分类号： C12N9/18
CPC分类号： C12N9/18 , C12P7/02 , C12P41/005
摘要： Polypeptide and recombinant protein having esterase activity which exhibit the amino acid sequence SEQ. ID. No. 1 and the use thereof.
摘要翻译：多肽和具有酯酶活性的重组蛋白质，其表现出氨基酸序列SEQ。 ID。 1及其用途。

3. 发明授权

US08617858B2 Method for the enzyme-catalysed hydrolysis of polyacrylic acid esters, and esterases used therefor 失效
标题翻译：聚丙烯酸酯的酶催化水解方法和用于其的酯酶的方法
公开(公告)号：US08617858B2
公开(公告)日：2013-12-31
申请号：US13003597
申请日：2009-07-17
申请人： Bernhard Hauer , Hans Wolfgang Höffken , Cecilia Kvarnström Branneby , Helmut Schwab , Sabine Feichtenhofer , Marlene Buchebner , Brigitte Pohn
发明人： Bernhard Hauer , Hans Wolfgang Höffken , Cecilia Kvarnström Branneby , Helmut Schwab , Sabine Feichtenhofer , Marlene Buchebner , Brigitte Pohn
IPC分类号： C12P7/52
CPC分类号： C12P7/62 , C08F8/12 , C12N9/16 , C12N9/18 , C12Y301/01001 , C08F120/18
摘要： The invention relates to a method for the enzyme-catalysed hydrolysis of polyacrylic acid esters. According to the method, at least one polyacrylic acid ester is provided and incubated with at least one enzyme selected from enzymes (EC 3.1) acting on ester bindings, until the ester groups contained in the polyacrylic acid ester are partially or fully hydrolytically split, and optionally the modified polymer obtained thereby is isolated. The invention also relates to the enzymes used and mutants thereof, nucleic acids coding for the enzymes, vectors comprising the nucleic acids, micro-organisms comprising the vectors, and the use of the enzymes, the vectors or the micro-organisms for carrying out a method for the enzyme-catalysed hydrolysis of polyacrylic acid esters. The present application also relates to polymer reaction products that can be obtained by the method, and methods for producing esterases.
摘要翻译：本发明涉及聚丙烯酸酯的酶催化水解方法。根据该方法，提供至少一种聚丙烯酸酯，并与至少一种选自作用于酯结合的酶（EC 3.1）的酶进行温育，直到聚丙烯酸酯中所含的酯基部分或完全水解分裂为止，以及由此分离获得的改性聚合物。本发明还涉及使用的酶及其突变体，编码酶的核酸，包含核酸的载体，包含载体的微生物，以及使用酶，载体或微生物进行酶聚丙烯酸酯酶催化水解的方法。本申请还涉及可以通过该方法获得的聚合物反应产物和生产酯酶的方法。

4. 发明授权

US08546112B2 Process for the stereoselective enzymatic hydrolysis of 5-methyl-3-nitromethyl-hexanoic acid ester 失效
标题翻译： 5-甲基-3-硝基甲基 - 己酸酯的立体选择性酶水解的方法
公开(公告)号：US08546112B2
公开(公告)日：2013-10-01
申请号：US12993132
申请日：2009-05-19
申请人： Martin Albert , Ferdinand Zepeck , Andreas Berger , Waander Riethorst , Helmut Schwab , Daniel Luschnig , Peter Remler , Joerg Salchenegger , Doris Osl , Dominic De Souza
发明人： Martin Albert , Ferdinand Zepeck , Andreas Berger , Waander Riethorst , Helmut Schwab , Daniel Luschnig , Peter Remler , Joerg Salchenegger , Doris Osl , Dominic De Souza
IPC分类号： C12P13/04
CPC分类号： C12P41/005 , C07B2200/07 , C07C67/343 , C07C201/12 , C07C205/51 , C07C227/04 , C07C229/08 , C07D207/46 , C12P13/008 , C07C69/533
摘要： The present invention relates to processes for the preparation of 5-methyl-3-nitromethyl-hexanoic acid ester and its salts. Also disclosed are processes for the preparation of 5-methyl-3-nitromethyl-hexanoic acid salt and a process for the preparation of 3-(aminomethyl)-5-methylhexanoic acid. (S)-5-Methyl-3-nitromethyl-hexanoic acid or (R)-5-methyl-3-nitromethyl-hexanoic acid in enantioenriched form or enantiopure form as well as salts thereof, (S)-5-methyl-3-nitromethyl-hexanoic acid ester or (R)-5-methyl-3-nitromethyl-hexanoic acid ester in enantioenriched form or enantiopure form and a compound, namely Formula (XIII), in racemic form, enantioenriched form or enantiopure form are also disclosed.
摘要翻译：本发明涉及5-甲基-3-硝基甲基 - 己酸酯及其盐的制备方法。还公开了制备5-甲基-3-硝基甲基 - 己酸盐的方法和制备3-（氨基甲基）-5-甲基己酸的方法。（S）-5-甲基-3-硝基甲基 - 己酸或（R）-5-甲基-3-硝基甲基 - 己酸，其具有对映体形式或对映体形式及其盐，（S）-5-甲基-3- - 硝基甲基 - 己酸酯或具有对映体形式或对映体形式的（R）-5-甲基-3-硝基甲基 - 己酸酯和式（XIII）化合物，外消旋形式，对映体形式或对映体形式也被公开。

5. 发明申请

US20120100582A1 PIG LIVER ESTERASES 有权
标题翻译：猪肝病
公开(公告)号：US20120100582A1
公开(公告)日：2012-04-26
申请号：US13265178
申请日：2010-04-26
申请人： Martin Kietzmann , Harald Pichler , Helmut Schwab , Amin El-Heliebi , Christine Winkler , Andreas Braun
发明人： Martin Kietzmann , Harald Pichler , Helmut Schwab , Amin El-Heliebi , Christine Winkler , Andreas Braun
IPC分类号： C12P7/62 , C12P7/02 , C12P7/40 , C12N9/18 , C12N15/55
CPC分类号： C12N9/18
摘要： The invention relates to an isolated polypeptide having esterase activity comprising an amino acid sequence shown in any one of SEQ ID NO's 2, 4, 6, 8, 10, 12 or 14 or a homologue thereof, comprising an amino acid substitution or deletion of one or more amino acids as shown in said SEQ ID NO's and resulting in a mutant polypeptide having an increased concentration of the fraction of the mutant polypeptide being present as an active and soluble protein in cleared lysate of the mutant polypeptide expressed in E. coli relative to the concentration of the fraction of the polypeptide without the mutation being present as an active and soluble protein in cleared lysate of the polypeptide without the one or more deletion or substitution expressed in E. coli under the same conditions. The invention also relates to nucleic acid encoding the polypeptides according to the invention, and the use of the polypeptides.
摘要翻译：本发明涉及具有酯酶活性的分离多肽，其包含SEQ ID NO：2,4,6,8,10,12或14中任一项所示的氨基酸序列或其同源物，其包含氨基酸取代或缺失的一个或更多的氨基酸，如所述SEQ ID NO所示，并且产生突变体多肽的浓度增加，所述突变体多肽作为活性和可溶性蛋白质存在于大肠杆菌中表达的突变多肽的澄清裂解物中，相对于未突变的多肽的级分的浓度作为活性和可溶性蛋白质存在于多肽的澄清裂解物中，而在相同条件下在大肠杆菌中不表达一个或多个缺失或取代。本发明还涉及编码根据本发明的多肽的核酸和多肽的用途。

6. 发明申请

US20060099696A1 Method for Producing Chiral Alpha-Hydroxycarboxylic Crystalline Acids 失效
标题翻译：生产手性α-羟基羧酸结晶酸的方法
公开(公告)号：US20060099696A1
公开(公告)日：2006-05-11
申请号：US10544103
申请日：2004-01-30
申请人： Herfried Griengl , Ingrid Osprian , Hans Schoemaker , Christoph Reisinger , Helmut Schwab
发明人： Herfried Griengl , Ingrid Osprian , Hans Schoemaker , Christoph Reisinger , Helmut Schwab
IPC分类号： C12P7/42 , C12N1/20
CPC分类号： C12P7/42
摘要： The invention relates to a method for producing chiral α-hydroxycarboxylic crystalline acids consisting in transforming cyanhydrins (R) or (S) into α-hydroxycarboxylic acids (R) or (S), respectively by enzymatic hydrolysis in the presence of Rhodococcus erythropolis NCIMB 11540.
摘要翻译：本发明涉及一种生产手性α-羟基羧酸结晶酸的方法，其通过在红球菌红霉素NCIMB 11540的存在下分别通过酶水解将氰尿酸（R）或（S）转化成α-羟基羧酸（R）或（S）。

7. 发明授权

US06861243B2 Genes containing a DNA sequence coding for hydroxynitrile lyase, recombinant proteins derived therefrom and having hydroxynitrile lyase activity, and use thereof 有权
标题翻译：含有编码羟基腈裂解酶的DNA序列的基因，由其衍生并具有羟基腈裂解酶活性的重组蛋白质及其用途
公开(公告)号：US06861243B2
公开(公告)日：2005-03-01
申请号：US10046232
申请日：2002-01-16
申请人： Helmut Schwab , Anton Glieder , Christoph Kratky , Ingrid Dreveny , Peter Pöchlauer , Wolfgang Skranc , Herbert Mayrhofer , Irma Wirth , Rudolf Neuhofer , Rodolfo Bona
发明人： Helmut Schwab , Anton Glieder , Christoph Kratky , Ingrid Dreveny , Peter Pöchlauer , Wolfgang Skranc , Herbert Mayrhofer , Irma Wirth , Rudolf Neuhofer , Rodolfo Bona
IPC分类号： C12N15/09 , C07K19/00 , C12N9/04 , C12N9/88 , C12N15/52 , C12N15/60 , C12P13/00
CPC分类号： C12P13/004 , C12N9/88 , C12N15/52
摘要： New genes containing a DNA sequence coding for hydroxynitrile lyase, which genes can be prepared via a primer combination based on the DNA sequence of the 5′-region of the mdl genes from Prunus serotina and from Prunus amygdalus and/or a primer 2 based on the 3′-region of the DNA sequences of one of the hydroxynitrile lyase isoenzymes from Prunus serotina or from Prunus amygdalus, subsequent amplification with a DNA polymerase using a DNA from organisms, containing genes coding for hydroxynitrile lyase, as templates and cloning, and also recombinant proteins which can be prepared in suitable host cells by heterologous expression of the DNA sequence of said HNL genes, and proteins and fusion proteins derived therefrom and use of said proteins for preparing (R)- or (S)-cyanohydrins.
摘要翻译：含有编码羟基腈裂解酶的DNA序列的新基因，该基因可以通过引物组合基于来自黑麦白浆和来自杏仁核的md1基因的5'区域的DNA序列和/或基于其中一个羟基腈裂解酶同工酶的一个来自山梨果皮或杏仁杏仁的DNA序列的3'区域，随后用含有编码羟基腈裂解酶的基因的来自生物体的DNA的DNA聚合酶扩增作为模板和克隆，以及可以通过异源表达所述HNL基因的DNA序列以及从其衍生的蛋白质和融合蛋白质以及所述蛋白质用于制备（R） - 或（S） - 氰基氢化物的方法，在合适的宿主细胞中制备重组蛋白质。

8. 发明申请

US20140199734A1 (R)- SELECTIVE AMINATION 审中-公开
标题翻译：（R） - 选择性氨基化
公开(公告)号：US20140199734A1
公开(公告)日：2014-07-17
申请号：US13810004
申请日：2011-07-14
申请人： Martin Schürmann , Wijnand Peter Helena Peeters , Natascha Hubertina Smeets , Helmut Schwab , Kerstin Steiner , Kateryna Mykoiayivna Lypetska , Gernot Strohmeier
发明人： Martin Schürmann , Wijnand Peter Helena Peeters , Natascha Hubertina Smeets , Helmut Schwab , Kerstin Steiner , Kateryna Mykoiayivna Lypetska , Gernot Strohmeier
IPC分类号： C12N9/10 , C12P13/00
CPC分类号： C12P13/001 , C12N9/1096 , C12Y206/01
摘要： The present invention relates to a method for the enzymatic synthesis of enantiomerically enriched (R)-amines of general formula [1][c], from the corresponding ketones of general formula [1][a], by using novel transaminases. These novel transaminases are selected from two different groups: either from a group of some 20 proteins with sequences as specified herein, or from a group of proteins having transaminase activity and isolated from a microorganism selected from the group of organisms consisting of Rahnella aquatilis, Ochrobactrum anthropi, Ochrobactrum tritici, Sinorhizobium morelense, Curtobacterium pusilllum, Paecilomyces lilacinus, Microbacterium ginsengisoli, Microbacterium trichothecenolyticum, Pseudomonas citronellolis, Yersinia kristensenii, Achromobacter spanius, Achromobacter insolitus, Mycobacterium fortuitum, Mycobacterium frederiksbergense, Mycobacterium sacrum, Mycobacterium fluoranthenivorans, Burkholderia sp., Burkholderia tropica, Cosmospora episphaeria, and Fusarium oxysporum.
摘要翻译：本发明涉及通过使用新型转氨酶从通式[1] [a]的相应酮酶促合成通式[1] [c]的对映异构体富集的（R） - 胺的方法。这些新型转氨酶选自两个不同的组：来自约20个具有本文所述序列的蛋白质的组，或来自具有转氨酶活性的一组蛋白质，并且从选自以下的微生物中分离：微生物，其由Rahnella aquatilis，Ochrobactrum anthropi，苍白杆菌小麦，苜蓿morelense，Curtobacterium属pusilllum，淡紫拟青霉，微ginsengisoli，微trichothecenolyticum，假单胞菌citronellolis，耶尔森kristensenii，无色spanius，无色insolitus，偶发分枝杆菌，结核分枝frederiksbergense，结核分枝骶骨，结核分枝fluoranthenivorans，鼻疽菌，类鼻疽tropica ，天蛾孢子和尖孢镰刀菌（Fusarium oxysporum）。

9. 发明授权

US07390647B2 Method for producing chiral α-hydroxycarboxylic crystalline acids 失效
标题翻译：生产手性α-羟基羧酸结晶酸的方法
公开(公告)号：US07390647B2
公开(公告)日：2008-06-24
申请号：US10544103
申请日：2004-01-30
申请人： Herfried Griengl , Ingrid Osprian , Hans Schoemaker , Christoph Reisinger , Helmut Schwab
发明人： Herfried Griengl , Ingrid Osprian , Hans Schoemaker , Christoph Reisinger , Helmut Schwab
IPC分类号： C12P41/00
CPC分类号： C12P7/42
摘要： The invention relates to a method for producing chiral α-hydroxycarboxylic crystalline acids consisting in transforming cyanhydrins (R) or (S) into α-hydroxycarboxylic acids (R) or (S), respectively by enzymatic hydrolysis in the presence of Rhodococcus erythropolis NCIMB 11540.
摘要翻译：本发明涉及一种生产手性α-羟基羧酸结晶酸的方法，其通过在红球菌红霉素NCIMB 11540的存在下分别通过酶水解将氰尿酸（R）或（S）转化成α-羟基羧酸（R）或（S）。

10. 发明申请

US20060199256A1 Method for producing chiral alpha-hydroxycarboxylic acids by enzymatic hydrolysis of chiral cyanohydrins 失效
公开(公告)号：US20060199256A1
公开(公告)日：2006-09-07
申请号：US11222020
申请日：2005-09-09
申请人： Herfried Griengl , Ingrid Osprian , Hans Schoemaker , Christoph Reisinger , Helmut Schwab
发明人： Herfried Griengl , Ingrid Osprian , Hans Schoemaker , Christoph Reisinger , Helmut Schwab
IPC分类号： C12N9/00 , C12N1/12
CPC分类号： C12P7/42
摘要： The invention relates to a method for producing chiral α-hydroxycarboxylic crystalline acids consisting in transforming cyanhydrins (R) or (S) into α-hydroxycarboxylic acids (R) or (S), respectively by enzymatic hydrolysis in the presence of Rhodococcus erythropolis NCIMB 11540.

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