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1. 发明公开

US20240284915A1 MICROBIAL FERMENTATION METHODS AND COMPOSITIONS 审中-公开
公开(公告)号：US20240284915A1
公开(公告)日：2024-08-29
申请号：US18649162
申请日：2024-04-29
申请人： NewLeaf Symbiotics, Inc.
发明人： GREGG BOGOSIAN
IPC分类号： A01N63/20 , C12N1/20 , C12N11/02 , C12N11/14 , C12N11/16
CPC分类号： A01N63/20 , C12N1/20 , C12N11/02 , C12N11/14 , C12N11/16
摘要： The present invention provides methods for the cultivation of the Methylobacterium genus of bacteria. In particular the method provides methods for the efficient and inexpensive cultivation of these bacteria. Additionally, the invention provides methods for the utilization of these bacterial cultures to improve plant agriculture.

2. 发明公开

US20240182935A1 METHOD OF PRODUCING & PROCESSING DIAMINES 审中-公开
公开(公告)号：US20240182935A1
公开(公告)日：2024-06-06
申请号：US18321585
申请日：2023-05-22
申请人： Genomatica, Inc.
发明人： Lauri H. Suominen , Connor J. Galleher , Michael Japs , Mark J. Burk , Cara Tracewell
IPC分类号： C12P13/00 , C12N1/02 , C12N1/20 , C12N9/88 , C12N9/96 , C12N11/16 , C12N15/52
CPC分类号： C12P13/001 , C12N1/02 , C12N1/20 , C12N9/88 , C12N9/96 , C12N11/16 , C12N15/52 , C12Y402/01001
摘要： Provided is a method of producing and isolating a diamine produced by microbial fermentation that minimizes undesirable salt formation to provide a lower cost process.

3. 发明申请

US20170002342A1 GRANULES COMPRISING ISOMALTULOSE SYNTHASE 审中-公开
标题翻译：包含异构体合成酶的颗粒
公开(公告)号：US20170002342A1
公开(公告)日：2017-01-05
申请号：US15123514
申请日：2015-03-03
申请人： Frank Hellmers , Thomas Hueller , Thomas Dassinger , Oliver Thum
发明人： Frank Hellmers , Thomas Hueller , Thomas Dassinger , Oliver Thum
IPC分类号： C12N9/98 , C12N9/90 , C08L33/12
CPC分类号： C12N9/98 , C08L33/04 , C08L33/12 , C12N9/90 , C12N11/16 , C12Y504/99011 , C08F220/14 , C08F2220/1808
摘要： The invention provides granules comprising A) at least one enzyme selected from at least one of the groups selected from transferases of EC 2, hydrolases of EC 3, lyases of EC 4 and isomerases of EC 5, B) at least one polymer selected from C1-C10-alkyl acrylate polymer, C1-C10-alkyl methacrylate polymer and C1-C10-alkyl acrylate-C1-C10-alkyl methacrylate copolymer, preferably C1-C10-alkyl acrylate-C1-C10-alkyl methacrylate copolymer and C) at least one inorganic carrier material.
摘要翻译：本发明涉及含有A）至少一种选自由EC2转移酶，EC 3水解酶，EC 4裂解酶和EC 5异构酶组成的组中的至少一种的B）至少一种选自C1 C 10 - 烷基丙烯酸酯聚合物，甲基丙烯酸C1-C10烷基酯聚合物和丙烯酸C1-C10烷基酯 - 甲基丙烯酸C1-C10烷基酯共聚物，优选C1-C10烷基丙烯酸酯-C1-C10-烷基甲基丙烯酸酯共聚物，C）至少一种无机载体材料。

4. 发明申请

US20150337256A1 Microbial Fermentation Methods and Compositions 审中-公开
标题翻译：微生物发酵方法和组成
公开(公告)号：US20150337256A1
公开(公告)日：2015-11-26
申请号：US14404922
申请日：2013-05-31
申请人： NewLeaf Symbiotics, Inc.
发明人： Gregg Bogosian
IPC分类号： C12N1/20 , A01N63/00
CPC分类号： C12N11/16 , A01N63/00 , A01N63/02 , C12N1/20 , C12N11/02 , C12N11/14 , A01N25/00 , A01N25/08
摘要： The present invention provides methods for the cultivation of the Methylobacterium genus of bacteria. In particular the method provides methods for the efficient and inexpensive cultivation of these bacteria. Additionally, the invention provides methods for the utilization of these bacterial cultures to improve plant agriculture.
摘要翻译：本发明提供了培养细菌的细杆菌属的方法。特别地，该方法提供了用于有效和廉价地培养这些细菌的方法。另外，本发明提供了利用这些细菌培养物来改善植物农业的方法。

5. 发明申请

US20130324407A1 Microbial Fermentation Methods and Compositions 有权
公开(公告)号：US20130324407A1
公开(公告)日：2013-12-05
申请号：US13907161
申请日：2013-05-31
申请人： NewLeaf Symbiotics, Inc.
发明人： Gregg Bogosian
IPC分类号： C12N11/02 , A01N63/00
CPC分类号： C12N11/16 , A01N63/00 , A01N63/02 , C12N1/20 , C12N11/02 , C12N11/14 , A01N25/00 , A01N25/08
摘要： The present invention provides methods for the cultivation of the Methylobacterium genus of bacteria. In particular the method provides methods for the efficient and inexpensive cultivation of these bacteria. Additionally, the invention provides methods for the utilization of these bacterial cultures to improve plant agriculture.

6. 发明申请

US20110233125A1 SYSTEMS AND METHODS OF CREATING A BIOFILM FOR THE REDUCTION OF WATER CONTAMINATION 审中-公开
标题翻译：制造生物膜以减少水污染的系统和方法
公开(公告)号：US20110233125A1
公开(公告)日：2011-09-29
申请号：US13032408
申请日：2011-02-22
申请人： Robert Gavin Jones , Gordon Alf Plishker
发明人： Robert Gavin Jones , Gordon Alf Plishker
IPC分类号： C02F3/34
CPC分类号： C12N1/20 , C02F3/108 , C02F3/341 , C02F3/348 , C12N11/16 , C12P39/00 , Y02W10/15
摘要： In some embodiments, a system may reduce contaminants in water. A system may include a biofilm in a container. The biofilm may be formed from one or more bacteria coupled to one or more substrates. The bacteria may be selected to maximize the reduction of contaminants in water. The system may include one or more bacteria generators to provide bacteria to the biofilm and/or one or more air sources to provide an air bubble stream to the container and/or the bacteria generator. In some embodiments, bacteria may be preserved in a starvation phase. Bacteria may be incubated until they reach a starvation phase. The bacteria may then be preserved as beads or immobilized on a substrate. The preserved bacteria may be used in a system for the reduction of contaminants in water.
摘要翻译：在一些实施方案中，系统可以减少水中的污染物。系统可以在容器中包括生物膜。生物膜可以由一种或多种与一种或多种底物偶联的细菌形成。可以选择细菌以最大限度地减少水中的污染物。该系统可以包括一个或多个细菌发生器以向生物膜和/或一个或多个空气源提供细菌以向容器和/或细菌发生器提供气泡流。在一些实施方案中，细菌可以在饥饿期保存。细菌可以孵育直至达到饥饿期。然后可以将细菌保存为珠粒或固定在基底上。保存的细菌可用于减少水中的污染物的系统中。

7. 发明申请

US20080138663A1 MICROBIAL ELECTRODE AND FUEL CELL AND SENSOR USING THE SAME 审中-公开
标题翻译：微电极和燃料电池及传感器
公开(公告)号：US20080138663A1
公开(公告)日：2008-06-12
申请号：US11947271
申请日：2007-11-29
申请人： Wataru Kubo , Tsuyoshi Nomoto
发明人： Wataru Kubo , Tsuyoshi Nomoto
IPC分类号： H01M8/16 , C25B11/06
CPC分类号： H01M8/16 , C12N11/16 , C12N13/00 , C12Q1/001 , C12Q1/02 , Y02E60/527
摘要： The present invention is directed to overcoming a problem of rate-limiting diffusion of a substrate toward an enzyme, which is a constituent of a microorganism, while ensuring proliferation of the microorganism, in a microbial electrode using a microorganism as an electrode catalyst. A microbial electrode is prepared using a microorganism expressing an enzyme on the surface layer of the cell membrane or cell wall thereof.
摘要翻译：本发明旨在克服在使用微生物作为电极催化剂的微生物电极的同时确保微生物的增殖的基板向作为微生物的成分的酶的扩散速率的限制的问题。使用表达酶的微生物在细胞膜或其细胞壁的表面层上制备微生物电极。

8. 发明申请

US20040175407A1 Microorganism coating components, coatings, and coated surfaces 审中-公开
标题翻译：微生物涂料组分，涂料和涂层表面
公开(公告)号：US20040175407A1
公开(公告)日：2004-09-09
申请号：US10792516
申请日：2004-03-03
申请人： REACTIVE SURFACES, LTD.
发明人： C. Steven McDaniel
IPC分类号： C12M001/34 , A61F002/00
CPC分类号： A62D5/00 , A62D3/02 , A62D2101/02 , A62D2101/26 , C09D5/00 , C09D5/34 , C09D7/63 , C09J11/02 , C12N9/16 , C12N11/08 , C12N11/16
摘要： Disclosed herein are novel paints and coatings comprising a cell-based particulate material. Specifically disclosed herein is cell-based particulate material prepared from microorganisms for use as a coating component. Also disclosed herein are methods of preparing a coating that comprises a cell-based particulate material.
摘要翻译：本文公开了包含基于细胞的颗粒材料的新型涂料和涂料。本文具体公开的是由用作涂层组分的微生物制备的基于细胞的颗粒材料。本文还公开了制备包含基于细胞的颗粒材料的涂层的方法。

9. 发明申请

US20040106118A1 Method for exposing peptides and polypeptides on the cell surface of bacteria 失效
标题翻译：在细菌细胞表面上暴露肽和多肽的方法
公开(公告)号：US20040106118A1
公开(公告)日：2004-06-03
申请号：US10415165
申请日：2003-10-15
发明人： Harald Kolmar , Andreas Christmann , Alexander Wentzel
IPC分类号： C12Q001/68
CPC分类号： C12N15/70 , C07K14/245 , C07K2319/00 , C12N11/16
摘要： The inventive method allows peptides or polypeptides to be exposed on the surface of gram-negative host bacteria using specific intimin-based anchor modules. Intimins with shortened carboxy terminals have been found to be particularly suitable anchor modules for passenger domains in the exterior E.coli cell membrane. According to said method, host bacteria are transformed using vectors, on which are located a fused nucleic acid sequence consisting of a sequence segment which codes for an intimin with a shortened carboxy terminal and a nucleic acid sequence segment which codes for the passenger peptide that is to be exposed. The invention permits a particularly large number of passenger domains to be exposed on the cell surface of the bacteria, without adversely affecting the viability of the bacteria.
摘要翻译：本发明的方法允许使用特定的基于Intimin的锚模块将肽或多肽暴露在革兰氏阴性宿主细菌的表面上。已经发现具有缩短的羧基末端的内皮素特别适用于外部大肠杆菌细胞膜中的乘客域的锚模块。根据所述方法，使用载体转化宿主细菌，所述载体位于由编码具有缩短的羧基末端的内含子的序列区段和由所述载体肽编码的核酸序列区段组成的融合核酸序列被暴露。本发明允许特别大量的乘客区域暴露在细菌的细胞表面上，而不会不利地影响细菌的存活力。

10. 发明授权

US06190662B1 Materials and methods relating to the attachment and display of substances on cell surfaces 失效
标题翻译：与细胞表面物质的附着和显示有关的材料和方法
公开(公告)号：US06190662B1
公开(公告)日：2001-02-20
申请号：US09036609
申请日：1998-03-06
申请人： Lothar Steidler , Erik Remaut , Jeremy Mark Wells
发明人： Lothar Steidler , Erik Remaut , Jeremy Mark Wells
IPC分类号： A61K3900
CPC分类号： C12N15/625 , A61K38/00 , A61K39/00 , C07K14/31 , C07K14/33 , C07K14/36 , C07K2319/00 , C07K2319/02 , C07K2319/035 , C07K2319/22 , C07K2319/55 , C12N11/16 , C12N15/746
摘要： Methods for obtaining surface expression of a desired protein or polypeptide in Gram-positive host organisms are provided. In addition, vectors useful in such methods as well as Gram-positive host organisms transformed with such vectors are disclosed.
摘要翻译：提供了在革兰氏阳性宿主生物中获得所需蛋白质或多肽表面表达的方法。此外，公开了可用于这些方法的载体以及用这些载体转化的革兰氏阳性宿主生物。

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