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1. 发明申请

US20060078872A1 Cell-preservation liquid 审中-公开
标题翻译：细胞保存液
公开(公告)号：US20060078872A1
公开(公告)日：2006-04-13
申请号：US11246366
申请日：2005-10-11
申请人： Atsushi Taguchi , Takeshi Sato , Noritsugu Yabe
发明人： Atsushi Taguchi , Takeshi Sato , Noritsugu Yabe
IPC分类号： A01N1/02
CPC分类号： A01N1/02 , A01N1/0226
摘要： A cell-preservation liquid containing at least a saccharide, sodium ion, and potassium ion, which is characterized in that the molar concentration of the contained sodium ion is equal to or more than that of the potassium ion is provided. The preservation liquid enables not only cryopreservation but also cold preservation, and an easy cell-preservation method may be provided. Moreover, when the cell-preservation liquid of the present invention is used, cell culture may be performed by inoculating cells in a medium with the cells suspended in the preservation liquid without a washing step or the like, so that the cells may be easily cultured.
摘要翻译：含有至少一种糖类，钠离子和钾离子的细胞保存液，其特征在于，含有的钠离子的摩尔浓度等于或大于钾离子的摩尔浓度。保存液不仅能够进行冷冻保存，而且能够进行冷藏保存，可以提供容易的保存细胞的方法。此外，当使用本发明的细胞保存液时，可以通过在没有洗涤步骤等的情况下将细胞接种在具有悬浮在保存液中的细胞的培养基中进行细胞培养，使得细胞可以容易地培养。

2. 发明授权

US5468635A In vitro method for stimulating cell growth by culturing cells in a serum-free culture medium comprising human lysozyme 失效
标题翻译：通过在包含人溶菌酶的无血清培养基中培养细胞来刺激细胞生长的体外方法
公开(公告)号：US5468635A
公开(公告)日：1995-11-21
申请号：US243074
申请日：1994-05-16
申请人： Koji Komiya , Tetsuya Takezono , Noritsugu Yabe , Hisao Matsui
发明人： Koji Komiya , Tetsuya Takezono , Noritsugu Yabe , Hisao Matsui
IPC分类号： C12N5/10 , C12N5/07 , C12N5/078 , C12N5/0783 , C12N5/09 , C12N5/16 , C12P21/00 , C12P21/08 , C12R1/91 , C12N5/00
CPC分类号： C12N5/0636 , C12N2500/90 , C12N2501/23 , C12N2501/70
摘要： The invention relates to an agent for promoting growth in animal cells. The agent contains a serum-free medium and lysozyme. The agent can optionally contain cytokines. The invention further relates to an in vitro method of stimulating the growth of T lymphoblastoid cells and peripheral blood lymphocytes, which comprises the step of culturing the T lymphoblastoid cells or peripheral blood lymphocytes in a serum-free culture medium comprising human lysozyme, as a growth stimulating agent, at a concentration of about 4-100 mg/liter.
摘要翻译：本发明涉及促进动物细胞生长的药剂。该试剂含有无血清培养基和溶菌酶。该试剂可任选地含有细胞因子。本发明还涉及刺激T淋巴母细胞细胞和外周血淋巴细胞生长的体外方法，其包括在包含人溶菌酶的无血清培养基中培养T淋巴母细胞细胞或外周血淋巴细胞作为生长的步骤浓度为约4-100mg /升的刺激剂。

3. 发明授权

US08815597B2 Baglike container for centrifugation and method of gene transfer using the same 有权
标题翻译：用于离心的袋状容器和使用其的基因转移方法
公开(公告)号：US08815597B2
公开(公告)日：2014-08-26
申请号：US12377234
申请日：2007-08-23
申请人： Hideto Chono , Junichi Mineno , Kazutoh Takesako , Takao Yoshida , Takashi Morimura , Kenji Sakai , Shin-ichi Yamada , Noritsugu Yabe , Yuko Taguchi
发明人： Hideto Chono , Junichi Mineno , Kazutoh Takesako , Takao Yoshida , Takashi Morimura , Kenji Sakai , Shin-ichi Yamada , Noritsugu Yabe , Yuko Taguchi
IPC分类号： C12N1/00 , C12M1/42 , C12N15/87 , A61J1/10 , C12M1/00 , C12M3/00 , C12N15/00 , C12P21/04 , C12P21/06 , B01L3/00
CPC分类号： A61J1/10 , B01L3/5021 , B01L3/505 , C12M23/14 , C12M35/04 , C12N15/87
摘要： The present invention provides a baglike container for centrifugation that is mounted in a centrifuge to thereby allow centrifugation of a dispersion liquid accommodated therein. The baglike container for centrifugation is less likely to tear or break as a result of centrifugation by disposing a container wall surface of the baglike container so as to apply centrifugal force perpendicular to the container wall surface.
摘要翻译：本发明提供一种用于离心的袋状容器，其安装在离心机中，从而允许其中容纳的分散液的离心。用于离心的袋状容器由于通过设置袋状容器的容器壁表面而离心而不易撕裂或破裂，从而施加垂直于容器壁表面的离心力。

4. 发明申请

US20100136680A1 BAGLIKE CONTAINER FOR CENTRIFUGATION AND METHOD OF GENE TRANSFER USING THE SAME 失效
标题翻译：用于离心的巴格莱集装箱和使用该方法的基因转移方法
公开(公告)号：US20100136680A1
公开(公告)日：2010-06-03
申请号：US12377234
申请日：2006-08-23
申请人： Hideto Chono , Junichi Mineno , Kazutoh Takesako , Takao Yoshida , Takashi Morimura , Kenji Sakai , Shin-ichi Yamada , Noritsugu Yabe , Yuko Taguchi
发明人： Hideto Chono , Junichi Mineno , Kazutoh Takesako , Takao Yoshida , Takashi Morimura , Kenji Sakai , Shin-ichi Yamada , Noritsugu Yabe , Yuko Taguchi
IPC分类号： C12N5/10 , B04B7/12
CPC分类号： A61J1/10 , B01L3/5021 , B01L3/505 , C12M23/14 , C12M35/04 , C12N15/87
摘要： Baglike container (1) that is fitted in a centrifuge to thereby allow centrifugation of any dispersion liquid placed therein. The baglike container for centrifugation is one comprising a main frame constituted of first container wall (3) with recess portion (6) formed so as to allow fitting in a centrifuge and with flange portion (7) provided on an open face border of the recess portion (6) and constituted of flexible second container wall (4) having its peripheral portion sealed mutually with the flange portion (7) of the first container wall (3) so as to define storing space (6′) for dispersion liquid in cooperation with the recess portion (6) and further comprising port (5) fitted to the main frame in a fashion allowing communication with the storing space (6′), wherein the recess portion (6) defining the storing space (6′) can be set in the centrifuge so that centrifugal force perpendicular to the bottom plane of the recess portion (6) applies. Moreover, there is disclosed a method of gene introduction using this baglike container.
摘要翻译：袋状容器（1），其装配在离心机中，从而允许离心分离任何分散液体。用于离心的袋状容器是包括由具有凹部（6）的第一容器壁（3）构成的主框架，所述第一容器壁（3）形成为能够安装在离心机中，并且具有设置在凹部的开放面边界上的凸缘部（7）部分（6），并且由具有与第一容器壁（3）的凸缘部分（7）相互密封的周边部分的柔性第二容器壁（4）构成，以便在合作中限定用于分散液体的储存空间（6'）与所述凹部（6）相连并且还包括以允许与所述存放空间（6'）连通的方式装配到所述主框架的端口（5），其中限定所述存储空间（6'）的所述凹部（6）可以设置在离心机中，使得垂直于凹部（6）的底面的离心力适用。此外，公开了使用该袋状容器的基因导入方法。

5. 发明授权

US5318906A Agent for stimulating growth of animal cells and serum-free medium containing same 失效
标题翻译：用于刺激动物细胞生长的试剂和含有该培养基的无血清培养基
公开(公告)号：US5318906A
公开(公告)日：1994-06-07
申请号：US893647
申请日：1992-06-04
申请人： Ko Sakata , Tetsuya Taekzono , Noritsugu Yabe , Hisao Matsui
发明人： Ko Sakata , Tetsuya Taekzono , Noritsugu Yabe , Hisao Matsui
IPC分类号： A61K35/14 , C12N5/02 , C12N5/0783 , C12N5/00 , A61K35/78 , C12P1/00 , C12Q1/00
CPC分类号： C12N5/0636 , A61K35/17 , A61K36/258 , A61K2039/5158 , C12N2500/76 , C12N2500/90 , C12N2501/23
摘要： An agent for stimulating growth of animal cells in vitro comprises ginseng or an active ingredient extracted from ginseng Serum-free culture media containing the agent are disclosed. The present serum-free media contain less impure protein than serum-containing media, so that isolation and purification of substances produced by animal cells cultured therein is facilitated. The present serum-free media enhance the growth of lymphocytes in vitro, and can be used for vital cell transplant therapy, particularly for adoptive immunotherapy.
摘要翻译：用于刺激体内动物细胞生长的试剂包括人参或从人参提取的活性成分公开了含有该药剂的无血清培养基。目前的无血清培养基含有比含血清培养基少的不纯的蛋白质，从而促进了在其中培养的动物细胞产生的物质的分离和纯化。目前的无血清培养基体外增强淋巴细胞的生长，可用于重要的细胞移植治疗，特别是过继免疫治疗。

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