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1. 发明授权

US09085798B2 Nucleic acid constructs and methods of use 有权
标题翻译：核酸构建体和使用方法
公开(公告)号：US09085798B2
公开(公告)日：2015-07-21
申请号：US13266568
申请日：2010-04-29
申请人： Mark S. Chee
发明人： Mark S. Chee
IPC分类号： C12P19/34 , C12Q1/68 , C07H21/02
CPC分类号： C12Q1/6869 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6834 , C12Q1/6874 , C12Q1/6876 , C12Q2563/179 , C12Q2563/149 , C12Q2539/103 , C12Q2537/143 , C12Q2525/155 , C12Q2525/131
摘要： The present invention provides oligonucleotide constructs, sets of such oligonucleotide constructs, and methods of using such oligonucleotide constructs to provide validated sequences or sets of validated sequences corresponding to desired ROIs. Such validated ROIs and constructs containing these have a wide variety of uses, including in synthetic biology, quantitative nucleic acid analysis, polymorphism and/or mutation screening, and the like.
摘要翻译：本发明提供寡核苷酸构建体，这样的寡核苷酸构建体的集合，以及使用这样的寡核苷酸构建体提供与期望的ROI相对应的验证序列或经验证序列的方法。这些验证的ROI和包含它们的构建体具有广泛的用途，包括合成生物学，定量核酸分析，多态性和/或突变筛选等。

2. 发明授权

US08906626B2 Multiplex nucleic acid reactions 有权
标题翻译：多重核酸反应
公开(公告)号：US08906626B2
公开(公告)日：2014-12-09
申请号：US13604872
申请日：2012-09-06
申请人： Arnold Oliphant , John R. Stuelpnagel , Mark S. Chee , Scott L. Butler , Jian-Bing Fan , Min-Jui Richard Shen
发明人： Arnold Oliphant , John R. Stuelpnagel , Mark S. Chee , Scott L. Butler , Jian-Bing Fan , Min-Jui Richard Shen
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6876 , C12Q1/6834 , C12Q1/6837 , C12Q2600/16 , Y10T436/143333 , C12Q2525/155 , C12Q2563/131 , C12Q2563/179
摘要： A method for detecting nucleic acids by (a) providing a sample having target nucleic acids, each nucleic acid having contiguous first, second, and third domains; (b) contacting the sample with probe sets to form hybridization complexes, wherein each probe set includes (i) a first probe having a sequence that is complementary to the first domain; and (ii) a second probe having a sequence substantially complementary to the third domain; (c) extending the first probes along the second domains of the complexes while the complexes are immobilized on a solid support; (d) ligating the extended first probes to the second probes to form templates; (e) amplifying the templates with primers that are complementary to the first and second priming sequences to produce amplicons; and (f) detecting the amplicons on the surface of a nucleic acid array.
摘要翻译：一种用于通过（a）提供具有靶核酸的样品来检测核酸的方法，每个核酸具有连续的第一，第二和第三结构域; （b）使样品与探针组接触以形成杂交复合物，其中每个探针组包括（i）具有与第一结构域互补的序列的第一探针; 和（ii）具有与第三结构域基本上互补的序列的第二探针; （c）沿络合物的第二结构域扩展第一探针，同时将复合物固定在固体支持物上; （d）将扩展的第一探针连接到第二探针以形成模板; （e）用与第一和第二引物序列互补的引物扩增模板以产生扩增子; 和（f）检测核酸阵列表面上的扩增子。

3. 发明授权

US08563246B2 Combinatorial decoding of random nucleic acid arrays 有权
公开(公告)号：US08563246B2
公开(公告)日：2013-10-22
申请号：US13532606
申请日：2012-06-25
申请人： Mark S. Chee , David R. Walt
发明人： Mark S. Chee , David R. Walt
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , B01J2219/005 , B01J2219/00572 , B01J2219/00585 , B01J2219/00596 , B01J2219/00648 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , C07B2200/11 , C07H21/00 , C12Q1/6837 , C40B20/04 , C40B40/06 , C40B70/00 , C12Q2563/161 , C12Q2563/185 , C12Q2525/179 , C12Q2563/155 , C12Q2565/514
摘要： Methods disclosed herein relate to identification of nucleotides in a nucleotide sequence.

4. 发明授权

US08288103B2 Multiplex nucleic acid reactions 有权
标题翻译：多重核酸反应
公开(公告)号：US08288103B2
公开(公告)日：2012-10-16
申请号：US12790757
申请日：2010-05-28
申请人： Arnold Oliphant , John R. Stuelpnagel , Mark S. Chee , Scott L. Butler , Jian-Bing Fan , Min-Jui Richard Shen
发明人： Arnold Oliphant , John R. Stuelpnagel , Mark S. Chee , Scott L. Butler , Jian-Bing Fan , Min-Jui Richard Shen
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6834 , C12Q1/6837 , C12Q2600/16 , Y10T436/143333 , C12Q2525/155 , C12Q2563/131 , C12Q2563/179
摘要： The invention is directed to a variety of multiplexing methods used to amplify and/or genotype a variety of samples simultaneously.
摘要翻译：本发明涉及用于同时扩增和/或基因型多种样品的多种多样化方法。

5. 发明申请

US20120164693A1 METHODS OF ENZYMATIC DISCRIMINATION ENHANCEMENT AND SURFACE-BOUND DOUBLE-STRANDED DNA 审中-公开
标题翻译：酶切鉴定增强和表面双重双链DNA的方法
公开(公告)号：US20120164693A1
公开(公告)日：2012-06-28
申请号：US11950213
申请日：2007-12-04
申请人： David J. Lockhart , Mark S. Chee , Dirk Vetter , Martin Digglemann
发明人： David J. Lockhart , Mark S. Chee , Dirk Vetter , Martin Digglemann
IPC分类号： C12P19/34 , C40B40/06 , C40B30/04
CPC分类号： C12N15/1093 , B01J19/0046 , B01J2219/00529 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00659 , B01J2219/00722 , C07B2200/11 , C07H21/00 , C12Q1/6827 , C12Q1/683 , C12Q1/6837 , C12Q1/6874 , C40B40/06 , C40B80/00 , G01N33/5308 , G01N33/551
摘要： Methods for discriminating between fully complementary hybrids and those that differ by one or more base pairs and libraries of unimolecular, double-stranded oligonucleotides on a solid support. In one embodiment, the present invention provides methods of using nuclease treatment to improve the quality of hybridization signals on high density oligonucleotide arrays. In another embodiment, the present invention provides methods of using ligation reactions to improve the quality of hybridization signals on high density oligonucleotide arrays. In yet another embodiment, the present invention provides libraries of unimolecular or intermolecular, double-stranded oligonucleotides on a solid support. These libraries are useful in pharmaceutical discovery for the screening of numerous biological samples for specific interactions between the double-stranded oligonucleotides, and peptides, proteins, drugs and RNA. In a related aspect, the present invention provides libraries of conformationally restricted probes on a solid support. The probes are restricted in their movement and flexibility using double-stranded oligonucleotides as scaffolding. The probes are also useful in various screening procedures associated with drug discovery and diagnosis. The present invention further provides methods for the preparation and screening of the above libraries.
摘要翻译：用于区分完全互补的杂交体与通过一个或多个碱基对不同的那些的方法和在固体支持物上的单分子双链寡核苷酸的文库。在一个实施方案中，本发明提供了使用核酸酶处理来提高高密度寡核苷酸阵列上杂交信号质量的方法。在另一个实施方案中，本发明提供了使用连接反应来提高高密度寡核苷酸阵列上杂交信号质量的方法。在另一个实施方案中，本发明提供了在固体支持物上的单分子或分子间双链寡核苷酸的文库。这些文库在药物发现中可用于筛选许多生物样品，用于双链寡核苷酸与肽，蛋白质，药物和RNA之间的特异性相互作用。在相关方面，本发明提供了在固体支持物上的构象限制探针的文库。使用双链寡核苷酸作为脚手架，探针的运动和灵活性受到限制。探针也可用于与药物发现和诊断相关的各种筛选程序。本发明还提供了制备和筛选上述文库的方法。

6. 发明申请

US20120129716A1 USE OF MICROFLUIDIC SYSTEMS IN THE DETECTION OF TARGET ANALYTES USING MICROSPHERE ARRAYS 有权
标题翻译：微流感系统在使用微波阵列检测目标分析仪时的应用
公开(公告)号：US20120129716A1
公开(公告)日：2012-05-24
申请号：US13299235
申请日：2011-11-17
申请人： Mark S. Chee , Todd A. Dickinson , Kevin Gunderson , Don O'Neil , John R. Stuelpnagel
发明人： Mark S. Chee , Todd A. Dickinson , Kevin Gunderson , Don O'Neil , John R. Stuelpnagel
IPC分类号： C40B30/04 , C40B60/12
CPC分类号： B01L3/502761 , B01L2200/0668 , B01L2300/0816 , B01L2300/0867 , B01L2400/0415 , C12Q1/68 , C12Q1/6816 , C12Q1/6869 , C12Q1/6874 , C12Q2565/629 , C12Q2563/149
摘要： The invention relates generally to methods and apparatus for conducting analyses, particularly microfluidic devices for the detection of target analytes.
摘要翻译：本发明一般涉及用于进行分析的方法和装置，特别是用于检测目标分析物的微流体装置。

7. 发明申请

US20110245101A1 CO-LOCALIZATION AFFINITY ASSAYS 审中-公开
标题翻译：共定位亲和力测定
公开(公告)号：US20110245101A1
公开(公告)日：2011-10-06
申请号：US13079878
申请日：2011-04-05
申请人： Mark S. Chee , Igor A. Kozlov , Anita D. Wentworth , Stephanie J. Kosakovsky Pond
发明人： Mark S. Chee , Igor A. Kozlov , Anita D. Wentworth , Stephanie J. Kosakovsky Pond
IPC分类号： C40B30/04
CPC分类号： G01N33/6845 , G01N2458/10
摘要： The invention provides a new assay format for high throughput molecular binding studies at a single molecule level. The invention enables creation of binding event identifiers in a highly parallel way. Individual binding events occur between two agents of a binding pair, e.g., a protein-based binding pair or a binding pair comprising a protein and a chemical moiety. The binding event identifier created through the binding of the two binding agents is unique to that pair, and identification of the binding event identifier is indicative of the binding of these specific may be assessed through a readout that is digital in nature. The invention enables very large sets of thousands or more of different binding agents or potential binding agents to be assayed simultaneously, resolving millions or more of potential interactions, and distinguishing specific interactions from those that are less specific.
摘要翻译：本发明提供了在单一分子水平上用于高通量分子结合研究的新的测定形式。本发明能够以高度并行的方式创建绑定事件标识符。个体结合事件发生在结合对的两个试剂之间，例如基于蛋白质的结合对或包含蛋白质和化学部分的结合对。通过两个绑定代理的绑定而创建的绑定事件标识符对于该对是唯一的，并且绑定事件标识符的标识指示可以通过本质上是数字的读出来评估这些特定的绑定。本发明使得能够同时测定成千上万或更多种不同结合剂或潜在的结合剂的非常大的组，解决数百万或更多的潜在相互作用，并且将特异性相互作用与不太具体的相互作用区分开来。

8. 发明授权

US08003354B2 Multiplex nucleic acid reactions 有权
公开(公告)号：US08003354B2
公开(公告)日：2011-08-23
申请号：US12032581
申请日：2008-02-15
申请人： Min-Jui Richard Shen , Arnold Oliphant , Scott L. Butler , John R. Stuelpnagel , Mark S. Chee , Kenneth M. Kuhn , Jian-Bing Fan
发明人： Min-Jui Richard Shen , Arnold Oliphant , Scott L. Butler , John R. Stuelpnagel , Mark S. Chee , Kenneth M. Kuhn , Jian-Bing Fan
IPC分类号： C12P19/34 , C12Q1/68 , C12M1/34 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6827 , C12Q1/6858 , C12Q2535/125 , C12Q2537/143 , C12Q2561/125 , C12Q2565/515
摘要： The invention is directed to a variety of multiplexing methods used to amplify and/or genotype a variety of samples simultaneously.

9. 发明申请

US20110143965A1 OLIGONUCLEOTIDE SYNTHESIZER 有权
标题翻译：寡核苷酸合成酶
公开(公告)号：US20110143965A1
公开(公告)日：2011-06-16
申请号：US13023406
申请日：2011-02-08
申请人： Michal Lebl , Mark S. Chee , Steven M. Barnard , David L. Heiner , Vit Pokorny , Mark J. Nibbe , Aaron Christopher Jones
发明人： Michal Lebl , Mark S. Chee , Steven M. Barnard , David L. Heiner , Vit Pokorny , Mark J. Nibbe , Aaron Christopher Jones
IPC分类号： C40B50/08 , C40B60/14
CPC分类号： B01J19/0046 , B01J2219/00283 , B01J2219/00286 , B01J2219/00295 , B01J2219/00308 , B01J2219/00315 , B01J2219/00326 , B01J2219/00328 , B01J2219/00369 , B01J2219/00389 , B01J2219/00412 , B01J2219/00421 , B01J2219/00423 , B01J2219/00454 , B01J2219/0049 , B01J2219/005 , B01J2219/00585 , B01J2219/0059 , B01J2219/00689 , B01J2219/0072 , B01J2219/00722 , C40B40/06 , C40B50/14 , C40B60/14 , G01N35/025 , G01N35/028 , G01N35/1067 , G01N2035/00504 , G01N2035/1037 , G01N2035/1069 , Y10T436/111666 , Y10T436/12 , Y10T436/2575
摘要： An apparatus for high-throughput combinatorial synthesis of organic molecules including a reaction vessel for containing a combinatorial chemistry synthetic reaction, a liquid dispenser for dispensing the liquid, a liquid aspirator and an adjustment mechanism. The reaction vessel includes an ingress aperture allowing a liquid to enter into an interior of the vessel and an egress aperture for aspirating the liquid from the vessel. The liquid dispenser dispenses liquid through the ingress aperture. The liquid aspirator aspirates liquid through the egress aperture and includes a rotor for carrying the vessel and orbiting the vessel about an axis of rotation. The rotor is oriented generally in a horizontal plane and includes an adjustment mechanism for adjusting the angle of the vessel relative to the horizontal plane in response to the centrifugal force generated by orbiting the vessel about the axis or rotation. A method of combinatorial synthesis of organic molecules is also disclosed.
摘要翻译：一种用于高通量组合合成有机分子的装置，包括用于包含组合化学合成反应的反应容器，用于分配液体的液体分配器，液体抽吸器和调节机构。反应容器包括允许液体进入容器内部的入口孔和用于从容器吸出液体的出口孔。液体分配器通过入口孔分配液体。液体吸入器通过出口孔吸出液体，并且包括用于承载容器并绕容器围绕旋转轴线运动的转子。转子通常在水平面定向，并且包括调节机构，用于响应于通过围绕轴线或旋转绕过容器而产生的离心力来调节容器相对于水平面的角度。还公开了组合合成有机分子的方法。

10. 发明授权

US07955794B2 Multiplex nucleic acid reactions 有权
标题翻译：多重核酸反应
公开(公告)号：US07955794B2
公开(公告)日：2011-06-07
申请号：US10177727
申请日：2002-06-20
申请人： Mun-Jui Richard Shen , Arnold Oliphant , Scott L. Butler , John E. Stuelpnagel , Mark S. Chee , Kenneth M. Kuhn , Jian-Bing Fan
发明人： Mun-Jui Richard Shen , Arnold Oliphant , Scott L. Butler , John E. Stuelpnagel , Mark S. Chee , Kenneth M. Kuhn , Jian-Bing Fan
IPC分类号： C12Q1/68 , C12P19/34 , C12M1/34 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6827 , C12Q1/6858 , C12Q2535/125 , C12Q2537/143 , C12Q2561/125 , C12Q2565/515
摘要： The invention is directed to a variety of multiplexing methods used to amplify and/or genotype a variety of samples simultaneously.
摘要翻译：本发明涉及用于同时扩增和/或基因型多种样品的多种多样化方法。

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