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1. 发明申请

US20120164693A1 METHODS OF ENZYMATIC DISCRIMINATION ENHANCEMENT AND SURFACE-BOUND DOUBLE-STRANDED DNA 审中-公开
标题翻译：酶切鉴定增强和表面双重双链DNA的方法
公开(公告)号：US20120164693A1
公开(公告)日：2012-06-28
申请号：US11950213
申请日：2007-12-04
申请人： David J. Lockhart , Mark S. Chee , Dirk Vetter , Martin Digglemann
发明人： David J. Lockhart , Mark S. Chee , Dirk Vetter , Martin Digglemann
IPC分类号： C12P19/34 , C40B40/06 , C40B30/04
CPC分类号： C12N15/1093 , B01J19/0046 , B01J2219/00529 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00659 , B01J2219/00722 , C07B2200/11 , C07H21/00 , C12Q1/6827 , C12Q1/683 , C12Q1/6837 , C12Q1/6874 , C40B40/06 , C40B80/00 , G01N33/5308 , G01N33/551
摘要： Methods for discriminating between fully complementary hybrids and those that differ by one or more base pairs and libraries of unimolecular, double-stranded oligonucleotides on a solid support. In one embodiment, the present invention provides methods of using nuclease treatment to improve the quality of hybridization signals on high density oligonucleotide arrays. In another embodiment, the present invention provides methods of using ligation reactions to improve the quality of hybridization signals on high density oligonucleotide arrays. In yet another embodiment, the present invention provides libraries of unimolecular or intermolecular, double-stranded oligonucleotides on a solid support. These libraries are useful in pharmaceutical discovery for the screening of numerous biological samples for specific interactions between the double-stranded oligonucleotides, and peptides, proteins, drugs and RNA. In a related aspect, the present invention provides libraries of conformationally restricted probes on a solid support. The probes are restricted in their movement and flexibility using double-stranded oligonucleotides as scaffolding. The probes are also useful in various screening procedures associated with drug discovery and diagnosis. The present invention further provides methods for the preparation and screening of the above libraries.
摘要翻译：用于区分完全互补的杂交体与通过一个或多个碱基对不同的那些的方法和在固体支持物上的单分子双链寡核苷酸的文库。在一个实施方案中，本发明提供了使用核酸酶处理来提高高密度寡核苷酸阵列上杂交信号质量的方法。在另一个实施方案中，本发明提供了使用连接反应来提高高密度寡核苷酸阵列上杂交信号质量的方法。在另一个实施方案中，本发明提供了在固体支持物上的单分子或分子间双链寡核苷酸的文库。这些文库在药物发现中可用于筛选许多生物样品，用于双链寡核苷酸与肽，蛋白质，药物和RNA之间的特异性相互作用。在相关方面，本发明提供了在固体支持物上的构象限制探针的文库。使用双链寡核苷酸作为脚手架，探针的运动和灵活性受到限制。探针也可用于与药物发现和诊断相关的各种筛选程序。本发明还提供了制备和筛选上述文库的方法。

2. 发明申请

US20100216656A1 METHODS OF ENZYMATIC DISCRIMINATION ENHANCEMENT AND SURFACE-BOUND DOUBLE-STRANDED DNA 有权
标题翻译：酶切鉴定增强和表面双重双链DNA的方法
公开(公告)号：US20100216656A1
公开(公告)日：2010-08-26
申请号：US12187503
申请日：2008-08-07
申请人： David J. Lockhart , Mark S. Chee , Dirk Vetter , Martin Digglemann
发明人： David J. Lockhart , Mark S. Chee , Dirk Vetter , Martin Digglemann
IPC分类号： C40B30/04 , C40B60/12
CPC分类号： C40B60/12 , B01J2219/00427 , B01J2219/00529 , B01J2219/00596 , B01J2219/00608 , B01J2219/00659 , B01J2219/00722 , C12Q1/6837 , C12Q1/6869 , C12Q2565/507 , C12Q2521/501
摘要： Methods for discriminating between fully complementary hybrids and those that differ by one or more base pairs and libraries of unimolecular, double-stranded oligonucleotides on a solid support. In one embodiment, the present invention provides methods of using nuclease treatment to improve the quality of hybridization signals on high density oligonucleotide arrays. In another embodiment, the present invention provides methods of using ligation reactions to improve the quality of hybridization signals on high density oligonucleotide arrays. In yet another embodiment, the present invention provides libraries of unimolecular or intermolecular, double-stranded oligonucleotides on a solid support. These libraries are useful in pharmaceutical discovery for the screening of numerous biological samples for specific interactions between the double-stranded oligonucleotides, and peptides, proteins, drugs and RNA. In a related aspect, the present invention provides libraries of conformationally restricted probes on a solid support. The probes are restricted in their movement and flexibility using double-stranded oligonucleotides as scaffolding. The probes are also useful in various screening procedures associated with drug discovery and diagnosis. The present invention further provides methods for the preparation and screening of the above libraries.
摘要翻译：用于区分完全互补的杂交体与通过一个或多个碱基对不同的那些的方法和在固体支持物上的单分子双链寡核苷酸的文库。在一个实施方案中，本发明提供了使用核酸酶处理来提高高密度寡核苷酸阵列上杂交信号质量的方法。在另一个实施方案中，本发明提供了使用连接反应来提高高密度寡核苷酸阵列上杂交信号质量的方法。在另一个实施方案中，本发明提供了在固体支持物上的单分子或分子间双链寡核苷酸的文库。这些文库在药物发现中可用于筛选许多生物样品，用于双链寡核苷酸与肽，蛋白质，药物和RNA之间的特异性相互作用。在相关方面，本发明提供了在固体支持物上的构象限制探针的文库。使用双链寡核苷酸作为脚手架，探针的运动和灵活性受到限制。探针也可用于与药物发现和诊断相关的各种筛选程序。本发明还提供了制备和筛选上述文库的方法。

3. 发明授权

US08236493B2 Methods of enzymatic discrimination enhancement and surface-bound double-stranded DNA 有权
标题翻译：酶鉴别增强方法和表面结合双链DNA
公开(公告)号：US08236493B2
公开(公告)日：2012-08-07
申请号：US12187503
申请日：2008-08-07
申请人： David J. Lockhart , Mark S. Chee , Martin Digglemann , Dirk Vetter
发明人： David J. Lockhart , Mark S. Chee , Martin Digglemann , Dirk Vetter
IPC分类号： C12Q1/68
CPC分类号： C40B60/12 , B01J2219/00427 , B01J2219/00529 , B01J2219/00596 , B01J2219/00608 , B01J2219/00659 , B01J2219/00722 , C12Q1/6837 , C12Q1/6869 , C12Q2565/507 , C12Q2521/501
摘要： Methods for discriminating between fully complementary hybrids and those that differ by one or more base pairs and libraries of unimolecular, double-stranded oligonucleotides on a solid support. In one embodiment, the present invention provides methods of using nuclease treatment to improve the quality of hybridization signals on high density oligonucleotide arrays. In another embodiment, the present invention provides methods of using ligation reactions to improve the quality of hybridization signals on high density oligonucleotide arrays. In yet another embodiment, the present invention provides libraries of unimolecular or intermolecular, double-stranded oligonucleotides on a solid support. These libraries are useful in pharmaceutical discovery for the screening of numerous biological samples for specific interactions between the double-stranded oligonucleotides, and peptides, proteins, drugs and RNA. In a related aspect, the present invention provides libraries of conformationally restricted probes on a solid support. The probes are restricted in their movement and flexibility using double-stranded oligonucleotides as scaffolding. The probes are also useful in various screening procedures associated with drug discovery and diagnosis. The present invention further provides methods for the preparation and screening of the above libraries.
摘要翻译：用于区分完全互补的杂交体与通过一个或多个碱基对不同的那些的方法和在固体支持物上的单分子双链寡核苷酸的文库。在一个实施方案中，本发明提供了使用核酸酶处理来提高高密度寡核苷酸阵列上杂交信号质量的方法。在另一个实施方案中，本发明提供了使用连接反应来提高高密度寡核苷酸阵列上杂交信号质量的方法。在另一个实施方案中，本发明提供了在固体支持物上的单分子或分子间双链寡核苷酸的文库。这些文库在药物发现中可用于筛选许多生物样品，用于双链寡核苷酸与肽，蛋白质，药物和RNA之间的特异性相互作用。在相关方面，本发明提供了在固体支持物上的构象限制探针的文库。使用双链寡核苷酸作为脚手架，探针的运动和灵活性受到限制。探针也可用于与药物发现和诊断相关的各种筛选程序。本发明还提供了制备和筛选上述文库的方法。

4. 发明授权

US06974666B1 Methods of enzymatic discrimination enhancement and surface-bound double-stranded DNA 失效
标题翻译：酶鉴别增强方法和表面结合双链DNA
公开(公告)号：US06974666B1
公开(公告)日：2005-12-13
申请号：US08533582
申请日：1995-10-18
申请人： David J. Lockhart , Mark S. Chee , Dirk Vetter , Martin Digglemann
发明人： David J. Lockhart , Mark S. Chee , Dirk Vetter , Martin Digglemann
IPC分类号： B01J19/00 , C07B61/00 , C07H21/00 , C12N15/10 , C12Q1/68 , C40B40/06 , G01N33/53 , G01N33/551 , C07H21/02 , C12P19/34
CPC分类号： C12N15/1093 , B01J19/0046 , B01J2219/00529 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00659 , B01J2219/00722 , C07B2200/11 , C07H21/00 , C12Q1/6827 , C12Q1/683 , C12Q1/6837 , C12Q1/6874 , C40B40/06 , C40B80/00 , G01N33/5308 , G01N33/551
摘要： Methods for discriminating between fully complementary hybrids and those that differ by one or more base pairs and libraries of unimolecular, double-stranded oligonucleotides on a solid support. In one embodiment, the present invention provides methods of using nuclease treatment to improve the quality of hybridization signals on high density oligonucleotide arrays. In another embodiment, the present invention provides methods of using ligation reactions to improve the quality of hybridization signals on high density oligonucleotide arrays. In yet another embodiment, the present invention provides libraries of unimolecular or intermolecular, double-stranded oligonucleotides on a solid support. These libraries are useful in pharmaceutical discovery for the screening of numerous biological samples for specific interactions between the double-stranded oligonucleotides, and peptides, proteins, drugs and RNA. In a related aspect, the present invention provides libraries of conformationally restricted probes on a solid support. The probes are restricted in their movement and flexibility using double-stranded oligonucleotides as scaffolding. The probes are also useful in various screening procedures associated with drug discovery and diagnosis. The present invention further provides methods for the preparation and screening of the above libraries.
摘要翻译：用于区分完全互补的杂交体与通过一个或多个碱基对不同的那些的方法和在固体支持物上的单分子双链寡核苷酸的文库。在一个实施方案中，本发明提供了使用核酸酶处理来提高高密度寡核苷酸阵列上杂交信号质量的方法。在另一个实施方案中，本发明提供了使用连接反应来提高高密度寡核苷酸阵列上杂交信号质量的方法。在另一个实施方案中，本发明提供了在固体支持物上的单分子或分子间双链寡核苷酸的文库。这些文库在药物发现中可用于筛选许多生物样品，用于双链寡核苷酸与肽，蛋白质，药物和RNA之间的特异性相互作用。在相关方面，本发明提供了在固体支持物上的构象限制探针的文库。使用双链寡核苷酸作为脚手架，探针的运动和灵活性受到限制。探针也可用于与药物发现和诊断相关的各种筛选程序。本发明还提供了制备和筛选上述文库的方法。

5. 发明申请

US20060292579A1 Methods of enzymatic discrimination enhancement and surface bound double-stranded DNA 审中-公开
标题翻译：酶鉴别增强方法和表面结合双链DNA
公开(公告)号：US20060292579A1
公开(公告)日：2006-12-28
申请号：US11176012
申请日：2005-07-05
申请人： David Lockhart , Mark Chee , Dirk Vetter , Martin Digglemann
发明人： David Lockhart , Mark Chee , Dirk Vetter , Martin Digglemann
IPC分类号： C12Q1/68
CPC分类号： C12N15/1093 , B01J19/0046 , B01J2219/00529 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00659 , B01J2219/00722 , C07B2200/11 , C07H21/00 , C12Q1/6827 , C12Q1/683 , C12Q1/6837 , C12Q1/6874 , C40B40/06 , C40B80/00 , G01N33/5308 , G01N33/551
摘要： Methods for discriminating between fully complementary hybrids and those that differ by one or more base pairs and libraries of unimolecular, double-stranded oligonucleotides on a solid support. In one embodiment, the present invention provides methods of using nuclease treatment to improve the quality of hybridization signals on high density oligonucleotide arrays. In another embodiment, the present invention provides methods of using ligation reactions to improve the quality of hybridization signals on high density oligonucleotide arrays. In yet another embodiment, the present invention provides libraries of unimolecular or intermolecular, double-stranded oligonucleotides on a solid support. These libraries are useful in pharmaceutical discovery for the screening of numerous biological samples for specific interactions between the double-stranded oligonucleotides, and peptides, proteins, drugs and RNA. In a related aspect, the present invention provides libraries of conformationally restricted probes on a solid support. The probes are restricted in their movement and flexibility using double-stranded oligonucleotides as scaffolding. The probes are also useful in various screening procedures associated with drug discovery and diagnosis. The present invention further provides methods for the preparation and screening of the above libraries.
摘要翻译：用于区分完全互补的杂交体与通过一个或多个碱基对不同的那些的方法和在固体支持物上的单分子双链寡核苷酸的文库。在一个实施方案中，本发明提供了使用核酸酶处理来提高高密度寡核苷酸阵列上杂交信号质量的方法。在另一个实施方案中，本发明提供了使用连接反应来提高高密度寡核苷酸阵列上杂交信号质量的方法。在另一个实施方案中，本发明提供了在固体支持物上的单分子或分子间双链寡核苷酸的文库。这些文库在药物发现中可用于筛选许多生物样品，用于双链寡核苷酸与肽，蛋白质，药物和RNA之间的特异性相互作用。在相关方面，本发明提供了在固体支持物上的构象限制探针的文库。使用双链寡核苷酸作为脚手架，探针的运动和灵活性受到限制。探针也可用于与药物发现和诊断相关的各种筛选程序。本发明还提供了制备和筛选上述文库的方法。

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