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    • 10. 发明申请
    • Method for determining nucleotide sequence of nucleic acid molecule by utilizing scanning probe microscope
    • 利用扫描探针显微镜测定核酸分子核苷酸序列的方法
    • US20060286573A1
    • 2006-12-21
    • US11386725
    • 2006-03-23
    • Kazuhiro Takada
    • Kazuhiro Takada
    • C12Q1/68
    • C12Q1/6869C12Q2565/601
    • A nucleotide sequence of a single-stranded nucleic acid molecule immobilized on a substrate is determined with an SPM by analyzing a shape of bases constituting the single-stranded nucleic acid molecule by scanning over a nucleic acid chain of the single-stranded nucleic acid molecule immobilized on the substrate with a probe provided to the SPM; preparing a standard nucleic acid-binding probe and detecting the presence or absence of interaction between the bases constituting the single-stranded nucleic acid molecule and the standard nucleic acid by scanning over the nucleic acid chain of the single-stranded nucleic acid molecule using the standard nucleic acid-binding probe; and determining the nucleotide sequence of the nucleic acid chain by identifying the kinds of bases existing in the nucleic acid chain constituting the single-stranded nucleic acid molecule from the results obtained above.
    • 通过在单链核酸分子的核酸链上扫描固定有单链核酸分子的核酸链,通过分析构成单链核酸分子的碱基的形状,用SPM测定固定在底物上的单链核酸分子的核苷酸序列 在具有提供给SPM的探针的衬底上; 制备标准核酸结合探针,并通过使用标准的核酸分子扫描单链核酸分子的核酸链,检测构成单链核酸分子的碱基与标准核酸之间是否存在相互作用 核酸结合探针; 根据上述结果,鉴定存在于构成单链核酸分子的核酸链中的碱基的种类,从而确定核酸链的核苷酸序列。