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1. 发明申请

US20070281312A1 NUCLEIC ACID-BINDING CHIPS FOR THE DETECTION OF PHOSPHATE DEFICIENCY CONDITIONS IN THE FRAMEWORK OF BIOPROCESS MONITORING 审中-公开
标题翻译：用于检测生物监测框架中磷酸盐缺乏条件的核酸结合剂
公开(公告)号：US20070281312A1
公开(公告)日：2007-12-06
申请号：US11764926
申请日：2007-06-19
申请人： Thomas SCHWEDER , Britta JURGEN , Stefan EVERS , Karl-Heinz MAURER , Le Thi HOI , Michael HECKER , Birgit VOIGT , Jorg FEESCHE
发明人： Thomas SCHWEDER , Britta JURGEN , Stefan EVERS , Karl-Heinz MAURER , Le Thi HOI , Michael HECKER , Birgit VOIGT , Jorg FEESCHE
IPC分类号： C12Q1/68 , C12M3/00
CPC分类号： C12Q1/689
摘要： The present application relates to nucleic acid-binding chips for monitoring bioprocesses, especially for detecting phosphate deficiency conditions. Said chips support probes for at least three of the following 47 genes: yhcR, tatCD, ctaC, gene for a putative acetoin reductase, spoIIGa, nasE, pstA, spoIIAA, gene for a hypothetical protein, yhbD, cotE, gene for a conserved hypothetical protein, yurl, spoVID, gene for a putative aromatic-specific dioxygenase, yhbE, gene for a putative benzoate transport protein, pstBB, spoIIIAH, gene for a hypothetical protein, spoIIQ, spoIIIAG, yvmA, gene for a putative ribonuclease, dhaS, yrbE, gene for a putative decarboxylase/dehydratase, htpG, yfkH, spoIIAB, spoIIIAF, alsD, gdh, yfkN, pstC, yfmQ, pstBA, dhaS homolog, gene for a putative phosphatase, phy, cypX, alsS, phoD, pstS, phoB, yvnA, yvmC, the total number of phosphate metabolism-specific different probes on the nucleic acid-binding chips not exceeding 100. The present application further relates to the use of corresponding gene probes, in particular on such chips, and to corresponding methods and possible uses.
摘要翻译：本申请涉及用于监测生物过程的核酸结合芯片，特别是用于检测磷酸盐缺乏条件。所述芯片支持以下47个基因中的至少三个基因的探针：yhcR，tatCD，ctaC，推定的乙偶姻还原酶基因，spoIIGa，nasE，pstA，spoIIAA，假设蛋白质的基因，yhbD，cotE，保守假设的基因蛋白质，yurl，spoVID，推定的芳香族特异性双加氧酶的基因，yhbE，推定的苯甲酸转运蛋白的基因，pstBB，spoIIIAH，假设蛋白质的基因，spoIIQ，spoIIIAG，yvmA，推定的核糖核酸酶的基因，dhaS，yrbE ，推定的脱羧酶/脱水酶的基因，htpG，yfkH，spoIIAB，spoIIIAF，alsD，gdh，yfkN，pstC，yfmQ，pstBA，dhaS同源物，推定的磷酸酶，phy，cypX，alsS，phoD，pstS，phoB， yvnA，yvmC，核酸结合芯片上的磷酸代谢特异性不同探针的总数不超过100.本申请还涉及相应的基因探针，特别是在这样的芯片上的应用，以及相应的方法和可能的用途。

2. 发明申请

US20070212693A1 FACTOR RECA FROM BACILLUS LICHENIFORMIS AND RECA-INACTIVATED SAFETY STEMS USED FOR BIOTECHNOLOGICAL PRODUCTION 审中-公开
标题翻译：生物技术生产生物反应器的生物反应器和回收失活的安全系统
公开(公告)号：US20070212693A1
公开(公告)日：2007-09-13
申请号：US10593425
申请日：2005-02-16
申请人： Jorg Feesche , Friedhelm Meinhardt , Hannes Nahrstedt , Jens Waldeck , Mark Grone , Renee Eichstadt
发明人： Jorg Feesche , Friedhelm Meinhardt , Hannes Nahrstedt , Jens Waldeck , Mark Grone , Renee Eichstadt
IPC分类号： C12Q1/68 , G01N33/554 , C07H21/04 , C12N1/21 , C12N15/74 , G01N33/569 , C07K14/32
CPC分类号： C07K14/32
摘要： The invention relates to the factor RecA from bacillus licheniformis DSM 13 (SEQ ID NO: 2), along with the associated gene recA (SEQ ID NO: 1), including related proteins and genes thereof, such as the variant indicated under SEQ ID NOS: 31 and 32, among others. According to the invention, gene recA is used for constructing gram-positive bacterial safety stems for biotechnological production, among other things, by inactivating the same in the respective stems. In a special embodiment, said stems are provided with additional functional deletions in phase-IV sporulation genes, preferably in gene SpoIV (in Bacillus licheniformis), gene yqfD (in B. subtilis), or the respective gene that is homologous thereto if said stems are naturally able to form spores. Furthermore, the inventive RecA represents a protein which can be used in molecular biological assays or for modulating the molecular biological activities of cells, especially in connection with DNA polymerization or recombination processes.
摘要翻译：本发明涉及来自地衣芽孢杆菌DSM13（SEQ ID NO：2）的因子RecA以及相关基因recA（SEQ ID NO：1），包括相关蛋白及其基因，例如SEQ ID NOS ：31和32等。根据本发明，基因recA用于构建用于生物技术生产的革兰氏阳性细菌安全茎，除其他外，通过在相应的茎中灭活它们。在一个特殊的实施方案中，所述茎在阶段-IV孢子形成基因中提供额外的功能缺失，优选在基因SpoIV（地衣芽孢杆菌），基因yqfD（枯草芽孢杆菌）中）或与其同源的相应基因如果所述茎自然能形成孢子。此外，本发明的RecA表示可用于分子生物学测定或调节细胞的分子生物学活性的蛋白质，特别是与DNA聚合或重组过程相关的蛋白质。

3. 发明申请

US20070190604A1 Novel Gene Products From Bacillus Licheniformis Forming Or Decomposing Polyamino Acids And Improved Biotechnological Production Methods Based Thereon 有权
标题翻译：来自地衣芽孢杆菌形成或分解聚氨基酸的新型基因产物及其改进的生物技术生产方法
公开(公告)号：US20070190604A1
公开(公告)日：2007-08-16
申请号：US11611945
申请日：2006-12-18
申请人： Jorg FEESCHE , Cornelius BESSLER , Stefan EVERS , Karl-Heinz MAURER , Armin EHRENREICH , Birgit VEITH , Heiko LIESEGANG , Anke HENNE , Christina HERZBERG , Gerhard GOTTSCHALK
发明人： Jorg FEESCHE , Cornelius BESSLER , Stefan EVERS , Karl-Heinz MAURER , Armin EHRENREICH , Birgit VEITH , Heiko LIESEGANG , Anke HENNE , Christina HERZBERG , Gerhard GOTTSCHALK
IPC分类号： C12P21/06 , C07H21/04 , C12N1/21 , C07K14/32 , C12N15/74
CPC分类号： C07K14/32 , C12N15/52 , C12P13/04
摘要： The invention relates to five or four novel genes and the gene products thereof from Bacillus licheniformis and sufficiently similar genes and proteins which are involved in vivo in the formation of polyamino acids. The gene in question is ywsC, ywsC′, ywtA, ywtB and ywtD or proteins coded thereby. The gene ywsC, ywsC′, ywtA and ywtB can be used to improve biotechnological production methods by microorganisms, wherein they are functionally inactivated; the gene ywtD which codes for a peptide decomposing poly-gamma glutamate can, inversely, contribute to the improvement of biotechnological production methods by increased expression. Said genes can be used positively, preferably to result in a modification or decomposition of poly-gamma glutamate.
摘要翻译：本发明涉及5或4种新型基因及其从地衣芽孢杆菌中得到的基因产物以及与体内在聚氨基酸形成有关的充分相似的基因和蛋白质。所讨论的基因是ywsC，ywsC'，ywtA，ywtB和ywtD或由此编码的蛋白质。基因ywsC，ywsC'，ywtA和ywtB可用于通过微生物改善生物技术生产方法，其中它们在功能上失活; 编码分解聚-γ谷氨酸的肽的基因ywtD可以相反地通过增加表达来促进生物技术生产方法的改进。所述基因可以积极使用，优选导致聚-γ谷氨酸的修饰或分解。

4. 发明申请

US20100112552A1 NUCLEIC ACID-BINDING CHIPS FOR DETECTING NITROGEN DEFICIENCIES AS PART OF BIOPROCESS CONTROL 审中-公开
标题翻译：用于检测氮缺乏症的核酸结合剂作为生物控制的一部分
公开(公告)号：US20100112552A1
公开(公告)日：2010-05-06
申请号：US11991761
申请日：2006-09-07
申请人： Stefan Evers , Jorg Feesche , Karl-Heinz Maurer , Thomas Schweder , Michael Hecker , Birgit Voigt , Britta Jurgen , Le Thi Hoi
发明人： Stefan Evers , Jorg Feesche , Karl-Heinz Maurer , Thomas Schweder , Michael Hecker , Birgit Voigt , Britta Jurgen , Le Thi Hoi
IPC分类号： C12Q1/68 , G01N33/00
CPC分类号： C12Q1/689 , C12Q1/6837 , C12Q2600/158
摘要： The invention relates to nucleic acid-binding chips for monitoring bioprocesses, specifically for detecting nitrogen deficiencies. Said chips carry probes that are sensitive to at least three of the following 50 genes: kdgR, citA, htrA, ycn1, yppF, trpB, ggt, alsR, glnA, nrgA, yciC, yvtA, nrgB, ycnJ, glnR, yvlA, yncE, yvlB, trpF, ydfS, trpD, ycnK, trpB, trpC, nasD, ycdH, nasC, nasB, trpE, pckA, nasF, yrkC, and tnrA or the homolgs to SEQ ID NO: 91, 41, 53, 19, 55, 47, 21, 17, 9, 85, 45, 49, 95, 63, 15, 93, or 81 at a maximum of 80 different probes that are specific of nitrogen metabolism. The invention also relates to the use of corresponding gene probes, especially on the aforementioned chips, to corresponding methods and possible uses.
摘要翻译：本发明涉及用于监测生物过程的核酸结合芯片，专门用于检测氮缺乏。所述芯片携带对以下50个基因中的至少三个基因敏感的探针：kdgR，citA，htrA，ycn1，yppF，trpB，ggt，alsR，glnA，nrgA，yciC，yvtA，nrgB，ycnJ，glnR，yv1A，yncE ，yvlB，trpF，ydfS，trpD，ycnK，trpB，trpC，nasD，ycdH，nasC，nasB，trpE，pckA，nasF，yrkC和tnrA或与SEQ ID NO：91,41,53,19,55 ，47,21,17,9,55,45,49,95,63,15,133或81，最多80个不同的氮代谢特异性探针。本发明还涉及相应的基因探针，特别是在上述芯片上的应用与相应的方法和可能的用途。

5. 发明申请

US20070190605A1 GENE PRODUCTS OF BACILLUS LICHENIFORMIS WHICH FORM ODOROUS SUBSTANCES AND IMPROVED BIOTECHNOLOGICAL PRODUCTION METHODS BASED THEREON 有权
标题翻译：基于生物技术的生物技术生产方法基于生物反应器的基因产物
公开(公告)号：US20070190605A1
公开(公告)日：2007-08-16
申请号：US11616319
申请日：2006-12-27
申请人： Cornelius Bessler , Jorg Feesche , Stefan Evers , Karl-Heinz Maurer , Armin Ehrenreich , Birgit Veith , Heiko Liesegang , Anke Henne , Christina Herzberg , Gerhard Gottschalk
发明人： Cornelius Bessler , Jorg Feesche , Stefan Evers , Karl-Heinz Maurer , Armin Ehrenreich , Birgit Veith , Heiko Liesegang , Anke Henne , Christina Herzberg , Gerhard Gottschalk
IPC分类号： C07H21/04 , C12P21/06 , C12P7/40 , C12N9/02 , C12N9/16 , C12N9/32 , C12N9/42 , C12N9/50 , C12N9/64 , C12N15/74 , C12N1/21
CPC分类号： C12N15/52 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/0016 , C12N9/1025 , C12N9/1029 , C12N9/1096 , C12N9/1217 , C12N9/16 , C12N9/78 , C12N9/80 , C12N9/84 , C12N9/88 , C12N9/93 , C12N15/75 , C12P7/16 , C12P7/40 , C12P7/42 , C12P7/52 , C12P13/001 , C12Y101/01 , C12Y101/01035 , C12Y101/01157 , C12Y102/04002 , C12Y103/99 , C12Y203/01019 , C12Y206/01042 , C12Y207/02007 , C12Y305/01001 , C12Y305/01011 , C12Y401/01018 , C12Y401/01019 , C12Y402/01017 , C12Y402/01055 , C12Y602/01 , C12Y602/01001 , Y02E50/10 , Y02P20/52
摘要： The present invention relates to 25 hitherto undescribed genes of B. licheniformis and gene products derived therefrom and all sufficiently homologous nucleic acids and proteins thereof. They occur in five different metabolic pathways for the formation of odorous substances. The metabolic pathways in question are for the synthesis of: 1) isovalerian acid (as part of the catabolism of leucine), 2) 2-methylbutyric acid and/or isobutyric acid (as part of the catabolism of valine and/or isoleucine), 3) butanol and/or butyric acid (as part of the metabolism of butyric acid), 4) propyl acid (as part of the metabolism of propionate) and/or 5) cadaverine and/or putrescine (as parts of the catabolism of lysine and/or arginine). The identification of these genes allows biotechnological production methods to be developed that are improved to the extent that, to assist these nucleic acids, the formation of the odorous substances synthesized via these metabolic pathways can be reduced by deactivating the corresponding genes in the micro-organism used for the biotechnological production. In addition, these gene products are thus available for preparing reactions or for methods according to their respective biochemical properties.
摘要翻译：本发明涉及地衣芽孢杆菌的25个迄今未描述的基因及其衍生的基因产物及其全部充分同源的核酸及其蛋白质。它们发生在五种不同的代谢途径中，用于形成气味物质。所讨论的代谢途径是用于合成：1）异戊酸（作为亮氨酸分解代谢的一部分），2）2-甲基丁酸和/或异丁酸（作为缬氨酸和/或异亮氨酸分解代谢的一部分） 3）丁醇和/或丁酸（作为丁酸代谢的一部分），4）丙酸（作为丙酸代谢的一部分）和/或5）尸胺和/或腐胺（作为赖氨酸分解代谢的一部分）和/或精氨酸）。这些基因的鉴定允许开发生物技术生产方法，其被改进到对于辅助这些核酸的程度，可以通过使微生物中相应的基因失活来减少通过这些代谢途径合成的恶臭物质的形成用于生物技术生产。此外，这些基因产物因此可用于制备反应或根据其各自生物化学性质的方法。

6. 发明申请

US20060057674A1 Translocating enzyme as a selection marker 审中-公开
标题翻译：转移酶作为选择标记
公开(公告)号：US20060057674A1
公开(公告)日：2006-03-16
申请号：US11216333
申请日：2005-08-31
申请人： Maren Hintz , Roland Freudl , Jorg Feesche , Roland Breves
发明人： Maren Hintz , Roland Freudl , Jorg Feesche , Roland Breves
IPC分类号： C12P21/06 , C07H21/04 , C12N9/00 , C12N15/74
CPC分类号： C12P21/02 , C12N9/14 , C12N15/74
摘要： The subject of the present invention is a selection system for microorganisms, which is based on the inactivation of an essential translocating enzyme and the curing of this inactivation by means of an indentically acting factor which is made available to the cells concerned by means of a vector. One important area of application for this system is includes processses for protein production by culturing cells of a microorganism strain that are characterized by this selection system, particularly such that the transgene of interest is located on the same vector performing the cure. Appropriate microorganisms, possible uses for genes of translocation enzymes and vectors are likewise presented, including in particular the use of the gene secA from gram-negative or gram-positive bacteria such as B. licheniformis.
摘要翻译：本发明的主题是微生物的选择系统，其基于对基本易位酶的失活和通过借助于载体可用于相关细胞的潜在作用因子来固化该失活。该系统的一个重要应用领域包括通过培养以该选择系统为特征的微生物菌株的细胞进行蛋白质生产的方法，特别是使感兴趣的转基因位于执行固化的相同载体上。同样提供适当的微生物，易位酶和载体基因的可能用途，特别包括来自革兰氏阴性或革兰氏阳性细菌如地衣芽孢杆菌的基因secA的使用。

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