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1. 发明申请

US20070281312A1 NUCLEIC ACID-BINDING CHIPS FOR THE DETECTION OF PHOSPHATE DEFICIENCY CONDITIONS IN THE FRAMEWORK OF BIOPROCESS MONITORING 审中-公开
标题翻译：用于检测生物监测框架中磷酸盐缺乏条件的核酸结合剂
公开(公告)号：US20070281312A1
公开(公告)日：2007-12-06
申请号：US11764926
申请日：2007-06-19
申请人： Thomas SCHWEDER , Britta JURGEN , Stefan EVERS , Karl-Heinz MAURER , Le Thi HOI , Michael HECKER , Birgit VOIGT , Jorg FEESCHE
发明人： Thomas SCHWEDER , Britta JURGEN , Stefan EVERS , Karl-Heinz MAURER , Le Thi HOI , Michael HECKER , Birgit VOIGT , Jorg FEESCHE
IPC分类号： C12Q1/68 , C12M3/00
CPC分类号： C12Q1/689
摘要： The present application relates to nucleic acid-binding chips for monitoring bioprocesses, especially for detecting phosphate deficiency conditions. Said chips support probes for at least three of the following 47 genes: yhcR, tatCD, ctaC, gene for a putative acetoin reductase, spoIIGa, nasE, pstA, spoIIAA, gene for a hypothetical protein, yhbD, cotE, gene for a conserved hypothetical protein, yurl, spoVID, gene for a putative aromatic-specific dioxygenase, yhbE, gene for a putative benzoate transport protein, pstBB, spoIIIAH, gene for a hypothetical protein, spoIIQ, spoIIIAG, yvmA, gene for a putative ribonuclease, dhaS, yrbE, gene for a putative decarboxylase/dehydratase, htpG, yfkH, spoIIAB, spoIIIAF, alsD, gdh, yfkN, pstC, yfmQ, pstBA, dhaS homolog, gene for a putative phosphatase, phy, cypX, alsS, phoD, pstS, phoB, yvnA, yvmC, the total number of phosphate metabolism-specific different probes on the nucleic acid-binding chips not exceeding 100. The present application further relates to the use of corresponding gene probes, in particular on such chips, and to corresponding methods and possible uses.
摘要翻译：本申请涉及用于监测生物过程的核酸结合芯片，特别是用于检测磷酸盐缺乏条件。所述芯片支持以下47个基因中的至少三个基因的探针：yhcR，tatCD，ctaC，推定的乙偶姻还原酶基因，spoIIGa，nasE，pstA，spoIIAA，假设蛋白质的基因，yhbD，cotE，保守假设的基因蛋白质，yurl，spoVID，推定的芳香族特异性双加氧酶的基因，yhbE，推定的苯甲酸转运蛋白的基因，pstBB，spoIIIAH，假设蛋白质的基因，spoIIQ，spoIIIAG，yvmA，推定的核糖核酸酶的基因，dhaS，yrbE ，推定的脱羧酶/脱水酶的基因，htpG，yfkH，spoIIAB，spoIIIAF，alsD，gdh，yfkN，pstC，yfmQ，pstBA，dhaS同源物，推定的磷酸酶，phy，cypX，alsS，phoD，pstS，phoB， yvnA，yvmC，核酸结合芯片上的磷酸代谢特异性不同探针的总数不超过100.本申请还涉及相应的基因探针，特别是在这样的芯片上的应用，以及相应的方法和可能的用途。

2. 发明申请

US20070190604A1 Novel Gene Products From Bacillus Licheniformis Forming Or Decomposing Polyamino Acids And Improved Biotechnological Production Methods Based Thereon 有权
标题翻译：来自地衣芽孢杆菌形成或分解聚氨基酸的新型基因产物及其改进的生物技术生产方法
公开(公告)号：US20070190604A1
公开(公告)日：2007-08-16
申请号：US11611945
申请日：2006-12-18
申请人： Jorg FEESCHE , Cornelius BESSLER , Stefan EVERS , Karl-Heinz MAURER , Armin EHRENREICH , Birgit VEITH , Heiko LIESEGANG , Anke HENNE , Christina HERZBERG , Gerhard GOTTSCHALK
发明人： Jorg FEESCHE , Cornelius BESSLER , Stefan EVERS , Karl-Heinz MAURER , Armin EHRENREICH , Birgit VEITH , Heiko LIESEGANG , Anke HENNE , Christina HERZBERG , Gerhard GOTTSCHALK
IPC分类号： C12P21/06 , C07H21/04 , C12N1/21 , C07K14/32 , C12N15/74
CPC分类号： C07K14/32 , C12N15/52 , C12P13/04
摘要： The invention relates to five or four novel genes and the gene products thereof from Bacillus licheniformis and sufficiently similar genes and proteins which are involved in vivo in the formation of polyamino acids. The gene in question is ywsC, ywsC′, ywtA, ywtB and ywtD or proteins coded thereby. The gene ywsC, ywsC′, ywtA and ywtB can be used to improve biotechnological production methods by microorganisms, wherein they are functionally inactivated; the gene ywtD which codes for a peptide decomposing poly-gamma glutamate can, inversely, contribute to the improvement of biotechnological production methods by increased expression. Said genes can be used positively, preferably to result in a modification or decomposition of poly-gamma glutamate.
摘要翻译：本发明涉及5或4种新型基因及其从地衣芽孢杆菌中得到的基因产物以及与体内在聚氨基酸形成有关的充分相似的基因和蛋白质。所讨论的基因是ywsC，ywsC'，ywtA，ywtB和ywtD或由此编码的蛋白质。基因ywsC，ywsC'，ywtA和ywtB可用于通过微生物改善生物技术生产方法，其中它们在功能上失活; 编码分解聚-γ谷氨酸的肽的基因ywtD可以相反地通过增加表达来促进生物技术生产方法的改进。所述基因可以积极使用，优选导致聚-γ谷氨酸的修饰或分解。

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