专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20060057674A1 Translocating enzyme as a selection marker 审中-公开
标题翻译：转移酶作为选择标记
公开(公告)号：US20060057674A1
公开(公告)日：2006-03-16
申请号：US11216333
申请日：2005-08-31
申请人： Maren Hintz , Roland Freudl , Jorg Feesche , Roland Breves
发明人： Maren Hintz , Roland Freudl , Jorg Feesche , Roland Breves
IPC分类号： C12P21/06 , C07H21/04 , C12N9/00 , C12N15/74
CPC分类号： C12P21/02 , C12N9/14 , C12N15/74
摘要： The subject of the present invention is a selection system for microorganisms, which is based on the inactivation of an essential translocating enzyme and the curing of this inactivation by means of an indentically acting factor which is made available to the cells concerned by means of a vector. One important area of application for this system is includes processses for protein production by culturing cells of a microorganism strain that are characterized by this selection system, particularly such that the transgene of interest is located on the same vector performing the cure. Appropriate microorganisms, possible uses for genes of translocation enzymes and vectors are likewise presented, including in particular the use of the gene secA from gram-negative or gram-positive bacteria such as B. licheniformis.
摘要翻译：本发明的主题是微生物的选择系统，其基于对基本易位酶的失活和通过借助于载体可用于相关细胞的潜在作用因子来固化该失活。该系统的一个重要应用领域包括通过培养以该选择系统为特征的微生物菌株的细胞进行蛋白质生产的方法，特别是使感兴趣的转基因位于执行固化的相同载体上。同样提供适当的微生物，易位酶和载体基因的可能用途，特别包括来自革兰氏阴性或革兰氏阳性细菌如地衣芽孢杆菌的基因secA的使用。

2. 发明申请

US20110129894A1 SECRETION OPTIMIZED MICROORGANISM 审中-公开
标题翻译：分泌优化微生物
公开(公告)号：US20110129894A1
公开(公告)日：2011-06-02
申请号：US12955219
申请日：2010-11-29
申请人： SANDRA SCHEELE , ROLAND FREUDL , JOHANNES BONGAERTS , KARL-HEINZ MAURER
发明人： SANDRA SCHEELE , ROLAND FREUDL , JOHANNES BONGAERTS , KARL-HEINZ MAURER
IPC分类号： C12N9/96 , C12N1/21
CPC分类号： C12P21/02 , C12N9/0006 , C12N15/63
摘要： Proteins having a cofactor can be secreted in an improved manner in a microorganism belonging to the genus Corynebacterium provided that the microorganism contains a nucleic acid sequence which is not naturally present in it and which comprises at least the following sequence sections: a) nucleic acid sequence coding for a protein which contains a cofactor, and b) a nucleic acid sequence which is at least 20% identical to the sequence given in SEQ ID NO. 1 or a nucleic acid sequence which is a structural homologue to this sequence, wherein the amino acid sequence which is encoded by the nucleic acid sequence b) functionally interacts with the amino acid sequence encoded by the nucleic acid sequence a) in such a manner that at least the amino acid sequence encoded by the nucleic acid sequence a) is excreted by the microorganism.
摘要翻译：具有辅因子的蛋白质可以以改进的方式分泌到属于棒杆菌属的微生物中，条件是微生物含有不天然存在的核酸序列，并且至少包含以下序列部分：a）核酸序列编码含有辅因子的蛋白质，和b）与SEQ ID NO：1中给出的序列至少20％相同的核酸序列。 1或与该序列结构同源的核酸序列，其中由核酸序列b）编码的氨基酸序列与由核酸序列a）编码的氨基酸序列功能性相互作用，使得至少由核酸序列a）编码的氨基酸序列被微生物排泄。

3. 发明授权

US07807808B2 Bacteria with increased levels of protein secretion, nucleotide sequences coding for a SecA protein with increased levels of protein secretion, and method for producing proteins 有权
标题翻译：蛋白质分泌水平升高的细菌，编码具有增加的蛋白质分泌水平的SecA蛋白质的核苷酸序列和产生蛋白质的方法
公开(公告)号：US07807808B2
公开(公告)日：2010-10-05
申请号：US12615209
申请日：2009-11-09
申请人： Oliver Koberling , Roland Freudl
发明人： Oliver Koberling , Roland Freudl
IPC分类号： C12N15/11 , C12N15/63 , C12N15/09 , C12N9/00 , C12N9/14 , C12N9/10 , C12N9/12 , C12N9/20 , C12N9/26 , C12N9/48 , C12N9/90 , C12P1/00 , C12P21/06 , C12P21/04 , C12P21/02 , C07H21/04
CPC分类号： C12N9/14
摘要： The invention relates to bacteria that have increased levels of protein secretion due to genetic modification, to nucleotide sequences and gene structures containing at least one gene coding for a SecA protein having increased levels of protein secretion, to a SecA having increased levels of protein secretion, and to a method for producing desired proteins using the inventive bacteria. The invention also relates to nucleic acids coding for a SecA protein having increased levels of protein secretion, containing a SecA gene sequence or allele, a SecA homologue or derivative, or nucleotide sequences hybridising therewith and comprising at least one mutation. Surprisingly, just one mutation in a nucleotide of a SecA gene leads to increased levels of protein secretion or to protein secretion for the first time.
摘要翻译：本发明涉及由于遗传修饰引起的蛋白质分泌水平升高的核酸序列和含有至少一种编码具有增加的蛋白质分泌水平的SecA蛋白质的基因的基因结构的细菌与具有增加的蛋白质分泌水平的SecA的细菌，以及使用本发明细菌产生所需蛋白质的方法。本发明还涉及编码具有增加的蛋白质分泌水平的SecA蛋白的核酸，其含有SecA基因序列或等位基因，SecA同源物或衍生物或与其杂交并包含至少一个突变的核苷酸序列。令人惊讶的是，SecA基因核苷酸中仅有一个突变首次导致蛋白质分泌水平升高或蛋白质分泌增加。

4. 发明申请

US20100184142A1 BACTERIA WITH INCREASED LEVELS OF PROTEIN SECRETION, NUCLEOTIDE SEQUENCES CODING FOR A SECA PROTEIN WITH INCREASED LEVELS OF PROTEIN SECRETION, AND METHOD FOR PRODUCING PROTEINS 有权
标题翻译：具有提高蛋白质分泌水平的细菌，具有提高的蛋白质分泌水平的SECA蛋白的核苷酸序列编码和用于生产蛋白质的方法
公开(公告)号：US20100184142A1
公开(公告)日：2010-07-22
申请号：US12615209
申请日：2009-11-09
申请人： Oliver Koberling , Roland Freudl
发明人： Oliver Koberling , Roland Freudl
IPC分类号： C12P21/00 , C12N15/31 , C12N15/63 , C12N1/21 , C12N9/00 , C12N9/48 , C12N9/26 , C12N9/16 , C12N9/90 , C12N9/10 , C12N9/12
CPC分类号： C12N9/14
摘要： The invention relates to bacteria that have increased levels of protein secretion due to genetic modification, to nucleotide sequences and gene structures containing at least one gene coding for a SecA protein having increased levels of protein secretion, to a SecA having increased levels of protein secretion, and to a method for producing desired proteins using the inventive bacteria. The invention also relates to nucleic acids coding for a SecA protein having increased levels of protein secretion and containing a gene sequence SecA or an allele, homologue or derivative of said nucleotide sequences or nucleotide sequences hybridising therewith and comprising at least one mutation. Surprisingly, just one mutation in a nucleotide of a SecA gene leads to increased levels of protein secretion or to protein secretion for the first time.
摘要翻译：本发明涉及由于遗传修饰引起的蛋白质分泌水平升高的核酸序列和含有至少一种编码具有增加的蛋白质分泌水平的SecA蛋白质的基因的基因结构的细菌与具有增加的蛋白质分泌水平的SecA的细菌，以及使用本发明细菌产生所需蛋白质的方法。本发明还涉及编码具有增加的蛋白质分泌水平并含有所述核苷酸序列的基因序列SecA或等位基因，同源物或衍生物或与其杂交的核苷酸序列并包含至少一个突变的SecA蛋白的核酸。令人惊讶的是，SecA基因核苷酸中仅有一个突变首次导致蛋白质分泌水平升高或蛋白质分泌增加。

5. 发明授权

US07655754B2 Bacteria with increased levels of protein secretion, nucleotide sequences coding for a SecA protein with increased levels of protein secretion, and methods for producing proteins 失效
标题翻译：具有增加的蛋白质分泌水平的细菌，编码具有增加的蛋白质分泌水平的SecA蛋白质的核苷酸序列和产生蛋白质的方法
公开(公告)号：US07655754B2
公开(公告)日：2010-02-02
申请号：US10554755
申请日：2004-05-28
申请人： Oliver Köberling , Roland Freudl
发明人： Oliver Köberling , Roland Freudl
IPC分类号： C07K14/00 , C12N9/00 , C12N9/10 , C12N9/12
CPC分类号： C12N9/14
摘要： The invention relates to bacteria that have increased levels of protein secretion due to genetic modification, to nucleotide sequences and gene structures containing at least one gene coding for a SecA protein having increased levels of protein secretion, to a SecA having increased levels of protein secretion, and to a method for producing desired proteins using the inventive bacteria. The invention also relates to nucleic acids coding for a SecA protein having increased levels of protein secretion, containing a SecA gene sequence or allele, a SecA homologue or derivative, or nucleotide sequences hybridising therewith and comprising at least one mutation. Surprisingly, just one mutation in a nucleotide of a SecA gene leads to increased levels of protein secretion or to protein secretion for the first time.
摘要翻译：本发明涉及由于遗传修饰引起的蛋白质分泌水平升高的核酸序列和含有至少一种编码具有增加的蛋白质分泌水平的SecA蛋白质的基因的基因结构的细菌与具有增加的蛋白质分泌水平的SecA的细菌，以及使用本发明细菌产生所需蛋白质的方法。本发明还涉及编码具有增加的蛋白质分泌水平的SecA蛋白的核酸，其含有SecA基因序列或等位基因，SecA同源物或衍生物或与其杂交并包含至少一个突变的核苷酸序列。令人惊讶的是，SecA基因核苷酸中仅有一个突变首次导致蛋白质分泌水平升高或蛋白质分泌增加。

6. 发明申请

US20070031836A1 Bacteria with increased levels of protein secretion, nucleotide sequences coding for a seca protein with increased levels of protein secretion, and methods for producing proteins 失效
标题翻译：具有增加的蛋白质分泌水平的细菌，编码具有增加的蛋白质分泌水平的Seca蛋白质的核苷酸序列和用于产生蛋白质的方法
公开(公告)号：US20070031836A1
公开(公告)日：2007-02-08
申请号：US10554755
申请日：2004-05-28
申请人： Oliver Koberling , Roland Freudl
发明人： Oliver Koberling , Roland Freudl
IPC分类号： C12Q1/68 , C07H21/04 , C12P21/06 , C12N1/21 , C07K14/32
CPC分类号： C12N9/14
摘要： The invention relates to bacteria that have increased levels of protein secretion due to genetic modification, to nucleotide sequences and gene structures containing at least one gene coding for a SecA protein having increased levels of protein secretion, to a SecA having increased levels of protein secretion, and to a method for producing desired proteins using the inventive bacteria. The invention also relates to nucleic acids coding for a SecA protein having increased levels of protein secretion and containing a gene sequence SecA or an allele, homologue or derivative of said nucleotide sequences or nucleotide sequences hybridising therewith and comprising at least one mutation. Surprisingly, just one mutation in a nucleotide of a SecA gene leads to increased levels of protein secretion or to protein secretion for the first time.
摘要翻译：本发明涉及由于遗传修饰引起的蛋白质分泌水平升高的核酸序列和含有至少一种编码具有增加的蛋白质分泌水平的SecA蛋白质的基因的基因结构的细菌与具有增加的蛋白质分泌水平的SecA的细菌，以及使用本发明细菌产生所需蛋白质的方法。本发明还涉及编码具有增加的蛋白质分泌水平并含有基因序列SecAi或所述核苷酸序列的等位基因，同系物或衍生物或与其杂交的核苷酸序列的SecA蛋白的核酸，至少一个突变。令人惊讶的是，SecA基因核苷酸中仅有一个突变首次导致蛋白质分泌水平升高或蛋白质分泌增加。

7. 发明授权

US5858727A Secretion of outer membrane proteins of gram-negative bacteria by means of gram-positive host organisms 失效
标题翻译：通过革兰氏阳性宿主生物分泌革兰氏阴性细菌的外膜蛋白
公开(公告)号：US5858727A
公开(公告)日：1999-01-12
申请号：US676378
申请日：1996-07-18
申请人： Jochen Meens , Michael Klose , Hermann Sahm , Roland Freudl
发明人： Jochen Meens , Michael Klose , Hermann Sahm , Roland Freudl
IPC分类号： A61K35/74 , A61K38/00 , A61K39/02 , A61P31/04 , C07H21/04 , C07K14/245 , C07K14/31 , C12N1/21 , C12N9/20 , C12N15/09 , C12N15/31 , C12N15/62 , C12P21/02 , G01N33/569 , C07K14/195
CPC分类号： C12N9/20 , C07K14/245 , C12N15/625 , G01N33/56911 , C07K2319/00 , C07K2319/02 , C07K2319/036 , Y10S530/825
摘要： The invention relates to a process for producing outer membrane proteins of gram-negative bacteria by gram-positive host cells. To this end, a gene structure containing a gene coded for an outer membrane protein is constructed; in front of this gene there is a pro-gene sequence which codes for a propeptide of an export protein of a gram-positive bacterium. In front of the pro-gene sequence there is a pre-gene sequence which codes for a signal peptide of an export protein of a gram-positive bacterium. After the cloning of a gene structure thus constructed into a vector and transformation into a gram-positive bacterium, the outer membrane protein is expressed with a signal peptide and propeptide, transported through the membrane of the gram-positive host cell and released into the test of the culture.
摘要翻译： PCT No.PCT / DE94 / 01394 Sec。 371日期：1996年7月18日 102（e）日期1996年7月18日PCT 1994年11月21日PCT PCT。 WO95 / 20048 PCT出版物日期1995年7月27日本发明涉及通过革兰氏阳性宿主细胞产生革兰氏阴性细菌的外膜蛋白的方法。为此，构建了含有外膜蛋白编码基因的基因结构体; 在该基因前面有一个pro-gene序列，其编码革兰氏阳性细菌的出口蛋白质的前肽。在前基因序列前面有一个前基因序列，其编码革兰氏阳性细菌的出口蛋白质的信号肽。克隆由此构建成载体并转化成革兰氏阳性细菌的基因结构后，外膜蛋白用信号肽和前肽表达，通过革兰氏阳性宿主细胞的膜转运并释放到测试中的文化。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式