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1. 发明授权

US08536099B2 Methods of bead manipulation and forming bead arrays 有权
标题翻译：珠粒操作和形成珠粒阵列的方法
公开(公告)号：US08536099B2
公开(公告)日：2013-09-17
申请号：US12872333
申请日：2010-08-31
申请人： Mark Oldham , George Fry , Christina Inman , John Bridgham , Timothy Hunkapillar , Charles Vann
发明人： Mark Oldham , George Fry , Christina Inman , John Bridgham , Timothy Hunkapillar , Charles Vann
IPC分类号： C40B30/00 , C40B30/04 , C40B30/08 , C40B30/10
CPC分类号： C12Q1/6869 , B01F13/0059 , B01F13/0222 , B01J19/0046 , B01J2219/00317 , B01J2219/00466 , B01J2219/00527 , B01J2219/00576 , B01J2219/00596 , B01J2219/00648 , B01J2219/00659 , B01J2219/00722 , B01L3/502707 , B01L3/50273 , B01L2200/027 , B01L2200/0647 , B01L2200/0684 , B01L2200/16 , B01L2300/0819 , B01L2300/0822 , B01L2300/0867 , B01L2300/0877 , B01L2300/0887 , B01L2300/1822 , B01L2400/0427 , C12Q1/6874 , G01N35/00069 , G01N35/1002 , G01N35/1097 , G01N2035/00148 , G01N2035/00237 , G01N2035/1034 , G02B21/34 , Y10T436/11 , Y10T436/143333
摘要： According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.
摘要翻译：根据各种实施方案，提供了一种方法，其包括用洗涤溶液洗涤基底上的DNA包被的珠粒阵列以从基底去除堆叠的珠粒。洗涤溶液可以在载体中包括惰性固体珠。 DNA包被的珠可以具有平均直径，并且洗涤溶液中的固体珠的平均直径可以是DNA包被的珠的直径的至少两倍。洗涤可以形成脱落的DNA包被的珠粒和单层DNA包被的珠粒。在一些实施方案中，用于形成阵列的第一珠粒与包含分子量约350Da或更低的PEG的聚（乙二醇）（PEG）溶液接触。在一些实施例中，用于形成珠阵列的滑块被提供为用于对其进行成像的系统。

2. 发明申请

US20090143244A1 System and apparatus for sequential processing of analytes 有权
公开(公告)号：US20090143244A1
公开(公告)日：2009-06-04
申请号：US11974239
申请日：2007-10-12
申请人： John Bridgham , Kevin Corcoran , George Golda , Michael C. Pallas , Sydney Brenner
发明人： John Bridgham , Kevin Corcoran , George Golda , Michael C. Pallas , Sydney Brenner
IPC分类号： C40B30/10 , C40B40/08 , C40B60/12
CPC分类号： C12Q1/6874 , B01J19/0046 , B01J2219/00274 , B01J2219/00317 , B01J2219/00648 , B01J2219/00659 , B01L3/502715 , B01L3/502761 , B01L2200/0647 , B01L2200/10 , B01L2300/0877 , B01L2400/0403 , B01L2400/0475 , B01L2400/0622 , B01L2400/0644 , C40B60/14 , G01N15/1456 , G01N15/1475 , G01N15/1484 , G01N21/253 , G01N2015/0015 , G01N2015/149 , C12Q2565/518 , C12Q2525/161 , C12Q2521/313 , C12Q2525/191
摘要： An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array. A key feature of the invention is the correlation of the sequence of optical signals generated by each microparticle in the planar array during the analytical process.

3. 发明授权

US06831994B2 System and apparatus for sequential processing of analytes 有权
标题翻译：用于顺序处理分析物的系统和设备
公开(公告)号：US06831994B2
公开(公告)日：2004-12-14
申请号：US09908130
申请日：2001-07-17
申请人： John Bridgham , Kevin Corcoran , George Golda , Michael C. Pallas , Sydney Brenner
发明人： John Bridgham , Kevin Corcoran , George Golda , Michael C. Pallas , Sydney Brenner
IPC分类号： G06K900
CPC分类号： C12Q1/6874 , B01J19/0046 , B01J2219/00274 , B01J2219/00317 , B01J2219/00648 , B01J2219/00659 , B01L3/502715 , B01L3/502761 , B01L2200/0647 , B01L2200/10 , B01L2300/0877 , B01L2400/0403 , B01L2400/0475 , B01L2400/0622 , B01L2400/0644 , C40B60/14 , G01N15/1456 , G01N15/1475 , G01N15/1484 , G01N21/253 , G01N2015/0015 , G01N2015/149 , C12Q2565/518 , C12Q2525/161 , C12Q2521/313 , C12Q2525/191
摘要： An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array. A key feature of the invention is the correlation of the sequence of optical signals generated by each microparticle in the planar array during the analytical process.
摘要翻译：提供了一种用于同时分析锚定到微粒的多个分析物的装置和系统。每个具有单一类型分析物的均匀群体的微粒被布置为在流动室内部基本上固定的平面阵列，其中分析过程的步骤通过将一系列处理试剂通过流体系统递送到微粒来进行，微处理器控制。响应于这样的处理步骤，在每个微粒的表面产生光信号，其特征在于微粒携带的分析物与递送的处理试剂之间的相互作用。通过收集和记录由平面阵列中的所有微粒产生的光学信号的图像来同时分析多个分析物。本发明的一个关键特征是在分析过程中由平面阵列中的每个微粒产生的光信号序列的相关性。

4. 发明授权

US06406848B1 Planar arrays of microparticle-bound polynucleotides 无效
标题翻译：微粒结合多核苷酸的平面阵列
公开(公告)号：US06406848B1
公开(公告)日：2002-06-18
申请号：US09424028
申请日：1999-11-16
申请人： John Bridgham , Kevin Corcoran , George Golda , Michael C. Pallas , Sydney Brenner
发明人： John Bridgham , Kevin Corcoran , George Golda , Michael C. Pallas , Sydney Brenner
IPC分类号： C12Q168
CPC分类号： C12Q1/6874 , B01J19/0046 , B01J2219/00274 , B01J2219/00317 , B01J2219/00648 , B01J2219/00659 , B01L3/502715 , B01L3/502761 , B01L2200/0647 , B01L2200/10 , B01L2300/0877 , B01L2400/0403 , B01L2400/0475 , B01L2400/0622 , B01L2400/0644 , C40B60/14 , G01N15/1456 , G01N15/1475 , G01N15/1484 , G01N21/253 , G01N2015/0015 , G01N2015/149 , C12Q2565/518 , C12Q2525/161 , C12Q2521/313 , C12Q2525/191
摘要： An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array. A key feature of the invention is the correlation of the sequence of optical signals generated by each microparticle in the planar array during the analytical process.
摘要翻译：提供了一种用于同时分析锚定到微粒的多个分析物的装置和系统。每个具有单一类型分析物的均匀群体的微粒被布置为在流动室内部基本上固定的平面阵列，其中分析过程的步骤通过将一系列处理试剂通过流体系统递送到微粒进行，微处理器控制。响应于这样的处理步骤，在每个微粒的表面产生光信号，其特征在于微粒携带的分析物与递送的处理试剂之间的相互作用。通过收集和记录由平面阵列中的所有微粒产生的光学信号的图像来同时分析多个分析物。本发明的一个关键特征是在分析过程中由平面阵列中的每个微粒产生的光信号序列的相关性。

5. 发明授权

US06806052B2 Planar arrays of microparticle-bound polynucleotides 有权
标题翻译：微粒结合多核苷酸的平面阵列
公开(公告)号：US06806052B2
公开(公告)日：2004-10-19
申请号：US10124884
申请日：2002-04-18
申请人： John Bridgham , Kevin P. Corcoran , George S. Golda , Michael C. Pallas , Sydney Brenner
发明人： John Bridgham , Kevin P. Corcoran , George S. Golda , Michael C. Pallas , Sydney Brenner
IPC分类号： C12Q168
CPC分类号： C12Q1/6874 , B01J19/0046 , B01J2219/00274 , B01J2219/00317 , B01J2219/00648 , B01J2219/00659 , B01L3/502715 , B01L3/502761 , B01L2200/0647 , B01L2200/10 , B01L2300/0877 , B01L2400/0403 , B01L2400/0475 , B01L2400/0622 , B01L2400/0644 , C40B60/14 , G01N15/1456 , G01N15/1475 , G01N15/1484 , G01N21/253 , G01N2015/0015 , G01N2015/149 , C12Q2565/518 , C12Q2525/161 , C12Q2521/313 , C12Q2525/191
摘要： An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array. A key feature of the invention is the correlation of the sequence of optical signals generated by each microparticle in the planar array during the analytical process.
摘要翻译：提供了一种用于同时分析锚定到微粒的多个分析物的装置和系统。每个具有单一类型分析物的均匀群体的微粒被布置为在流动室内部基本上固定的平面阵列，其中分析过程的步骤通过将一系列处理试剂通过流体系统递送到微粒进行，微处理器控制。响应于这样的处理步骤，在每个微粒的表面产生光信号，其特征在于微粒携带的分析物与递送的处理试剂之间的相互作用。通过收集和记录由平面阵列中的所有微粒产生的光学信号的图像来同时分析多个分析物。本发明的一个关键特征是在分析过程中由平面阵列中的每个微粒产生的光信号序列的相关性。

6. 发明授权

US4816513A Automated polypeptide synthesis process 失效
标题翻译：自动多肽合成过程
公开(公告)号：US4816513A
公开(公告)日：1989-03-28
申请号：US53324
申请日：1987-05-22
申请人： John Bridgham , Timothy G. Geiser , Michael W. Hunkapiller , Stephen B. H. Kent , Mark P. Marriott , Paul O. Ramstad , Eric S. Nordman
发明人： John Bridgham , Timothy G. Geiser , Michael W. Hunkapiller , Stephen B. H. Kent , Mark P. Marriott , Paul O. Ramstad , Eric S. Nordman
IPC分类号： C07K1/00 , B01J19/00 , C07K1/04 , C40B40/10 , C40B60/14 , C08L89/00 , C07C103/52
CPC分类号： C07K1/045 , B01J19/0046 , B01J2219/00286 , B01J2219/00389 , B01J2219/00484 , B01J2219/00488 , B01J2219/0049 , B01J2219/00495 , B01J2219/005 , B01J2219/0059 , B01J2219/00596 , B01J2219/00689 , B01J2219/00695 , B01J2219/00725 , C40B40/10 , C40B60/14
摘要： An apparatus is provided for automatically constructing a polypeptide of high purity, up to 50 amino acids in length, using only single couplings. The apparatus includes an activation system for receiving protected amino acids, one kind at a time, having a common vessel (an activator vessel) in which to activate each of the amino acids. Also included is a reaction vessel for containing a resin used in solid-phase peptide synthesis for attaching a peptide chain thereto. A transfer system is also provided, which operates under control of a computer, to transfer the activated species from the activation system to the reaction vessel and to transfer amino acids, reagents, gases, and solvents from one part of the apparatus to another. The activator system also includes a temperature controlled concentrator vessel in which an activator solvent is replaced by a coupling solvent to enhance the coupling of the activated species to the peptide chain in the reaction vessel. Also included in the synthesizer system is a vortexer for affecting total washing of materials in the reaction vessel and the reaction vessel itself, an automated peptide resin sampling system, and an autodelivery system for providing individual containers of amino acid to the synthesizer in the order desired in the peptide sequence. A liquid sensor system is also included to monitor transitions between gases and liquids in specific tubes in the synthesizer in order to provide input signals to the computer system for control purposes. The computer system software which controls the operation of the synthesizer is organized according to a series of menus which allows the user of the system to select individual cycles of operation for each vessel in the synthesizer. In addition, an algorithm has been developed which provides for optimum efficiency in the production of a peptide for any given selection of cycles.
摘要翻译：提供了一种用于仅使用单个耦合自动构建高纯度多达50个氨基酸长度的多肽的装置。该装置包括用于一次接收受保护的氨基酸的激活系统，其具有在其中激活每个氨基酸的共同血管（活化剂容器）。还包括用于含有固相肽合成中用于连接肽链的树脂的反应容器。还提供了在计算机控制下操作的转移系统，以将活化物质从活化系统转移到反应容器中，并将氨基酸，试剂，气体和溶剂从设备的一部分转移到另一部分。活化剂体系还包括温度控制的浓缩容器，其中活化剂溶剂被偶联溶剂代替以增强活化物质与反应容器中的肽链的偶联。还包括在合成器系统中的是用于影响反应容器和反应容器本身中的材料的全部洗涤的涡流器，自动化肽树脂取样系统和用于按合适的顺序向合成器提供单个氨基酸容器的自动输送系统在肽序列中。还包括液体传感器系统以监测合成器中特定管中的气体和液体之间的转换，以便为控制目的向计算机系统提供输入信号。控制合成器操作的计算机系统软件根据一系列菜单进行组织，这些菜单允许系统的用户为合成器中的每个容器选择各个操作周期。此外，已经开发了一种算法，其提供用于任何给定选择周期的肽的生产的最佳效率。

7. 发明申请

US20110136677A1 METHODS OF BEAD MANIPULATION AND FORMING BEAD ARRAYS 有权
标题翻译：珠子操纵和形成珠粒阵列的方法
公开(公告)号：US20110136677A1
公开(公告)日：2011-06-09
申请号：US12872333
申请日：2010-08-31
申请人： Mark OLDHAM , George Fry , Christina Inman , John Bridgham , Timothy Hunkapillar , Charles Vann
发明人： Mark OLDHAM , George Fry , Christina Inman , John Bridgham , Timothy Hunkapillar , Charles Vann
IPC分类号： C40B20/08 , C40B50/00 , C40B30/00 , B05D5/00 , B05D7/24
CPC分类号： C12Q1/6869 , B01F13/0059 , B01F13/0222 , B01J19/0046 , B01J2219/00317 , B01J2219/00466 , B01J2219/00527 , B01J2219/00576 , B01J2219/00596 , B01J2219/00648 , B01J2219/00659 , B01J2219/00722 , B01L3/502707 , B01L3/50273 , B01L2200/027 , B01L2200/0647 , B01L2200/0684 , B01L2200/16 , B01L2300/0819 , B01L2300/0822 , B01L2300/0867 , B01L2300/0877 , B01L2300/0887 , B01L2300/1822 , B01L2400/0427 , C12Q1/6874 , G01N35/00069 , G01N35/1002 , G01N35/1097 , G01N2035/00148 , G01N2035/00237 , G01N2035/1034 , G02B21/34 , Y10T436/11 , Y10T436/143333
摘要： According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.
摘要翻译：根据各种实施方案，提供了一种方法，其包括用洗涤溶液洗涤基底上的DNA包被的珠粒阵列以从基底去除堆叠的珠粒。洗涤溶液可以在载体中包括惰性固体珠。 DNA包被的珠可以具有平均直径，并且洗涤溶液中的固体珠的平均直径可以是DNA包被的珠的直径的至少两倍。洗涤可以形成脱落的DNA包被的珠粒和单层DNA包被的珠粒。在一些实施方案中，用于形成阵列的第一珠粒与包含分子量约350Da或更低的PEG的聚（乙二醇）（PEG）溶液接触。在一些实施例中，用于形成珠阵列的滑块被提供为用于对其进行成像的系统。

8. 发明申请

US20100111768A1 SYSTEMS AND DEVICES FOR SEQUENCE BY SYNTHESIS ANALYSIS 有权
标题翻译：通过合成分析进行序列的系统和设备
公开(公告)号：US20100111768A1
公开(公告)日：2010-05-06
申请号：US12295337
申请日：2007-03-30
申请人： Saibal Banerjee , Colin Barnes , Kevin Benson , John Bridgham , Jason Bryant , Dale Buermann , Sergey Etchin , Jonny Ho , Xavier Lee , Peter Lundberg , Klaus Maisinger , Bojan Obradovic , Mark Pratt , Isabelle Rasolonjatovo , Mark Reed , Chiara Rodighiero , Subra Sankar , Gary Schroth , Ning Sizto , Harold Swerdlow , Eric Vermaas
发明人： Saibal Banerjee , Colin Barnes , Kevin Benson , John Bridgham , Jason Bryant , Dale Buermann , Sergey Etchin , Jonny Ho , Xavier Lee , Peter Lundberg , Klaus Maisinger , Bojan Obradovic , Mark Pratt , Isabelle Rasolonjatovo , Mark Reed , Chiara Rodighiero , Subra Sankar , Gary Schroth , Ning Sizto , Harold Swerdlow , Eric Vermaas
IPC分类号： C12Q1/68 , C12M3/00
CPC分类号： C12Q1/6869 , C12Q2535/122 , C12Q2565/60 , G01N21/6456
摘要： The present invention comprises systems and devices for sequencing of nucleic acid, such as short DNA sequences from clonally amplified single-molecule arrays.
摘要翻译：本发明包括用于核酸测序的系统和装置，例如来自克隆扩增的单分子阵列的短DNA序列。

9. 发明授权

US07282370B2 System and apparatus for sequential processing of analytes 有权
标题翻译：用于顺序处理分析物的系统和设备
公开(公告)号：US07282370B2
公开(公告)日：2007-10-16
申请号：US11207443
申请日：2005-08-18
申请人： John Bridgham , Kevin Corcoran , George Golda , Michael C. Pallas , Sydney Brenner
发明人： John Bridgham , Kevin Corcoran , George Golda , Michael C. Pallas , Sydney Brenner
IPC分类号： G01N21/76 , G01N21/00 , G01N37/00
CPC分类号： G01N21/253 , B01J2219/00274 , B01L3/5027 , B01L3/502715 , B01L3/502761 , B01L2200/0647 , B01L2200/10 , B01L2300/0877 , B01L2400/0403 , B01L2400/0475 , B01L2400/0622 , B01L2400/0644 , G01N2015/144 , G01N2015/1452 , G01N2035/00158 , Y10S435/81 , Y10T436/13 , Y10T436/143333
摘要： An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array. A key feature of the invention is the correlation of the sequence of optical signals generated by each microparticle in the planar array during the analytical process.
摘要翻译：提供了一种用于同时分析锚定到微粒的多个分析物的装置和系统。每个具有单一类型分析物的均匀群体的微粒被布置为在流动室内部基本上固定的平面阵列，其中分析过程的步骤通过将一系列处理试剂通过流体系统递送到微粒进行，微处理器控制。响应于这样的处理步骤，在每个微粒的表面产生光信号，其特征在于微粒携带的分析物与递送的处理试剂之间的相互作用。通过收集和记录由平面阵列中的所有微粒产生的光学信号的图像来同时分析多个分析物。本发明的一个关键特征是在分析过程中由平面阵列中的每个微粒产生的光信号序列的相关性。

10. 发明申请

US20060051876A1 System and apparatus for sequential processing of analytes 有权
公开(公告)号：US20060051876A1
公开(公告)日：2006-03-09
申请号：US11207443
申请日：2005-08-18
申请人： John Bridgham , Kevin Corcoran , George Golda , Michael Pallas , Sydney Brenner
发明人： John Bridgham , Kevin Corcoran , George Golda , Michael Pallas , Sydney Brenner
IPC分类号： G01N33/50
CPC分类号： G01N21/253 , B01J2219/00274 , B01L3/5027 , B01L3/502715 , B01L3/502761 , B01L2200/0647 , B01L2200/10 , B01L2300/0877 , B01L2400/0403 , B01L2400/0475 , B01L2400/0622 , B01L2400/0644 , G01N2015/144 , G01N2015/1452 , G01N2035/00158 , Y10S435/81 , Y10T436/13 , Y10T436/143333
摘要： An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array. A key feature of the invention is the correlation of the sequence of optical signals generated by each microparticle in the planar array during the analytical process.

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