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    • 4. 发明授权
    • Production of zeaxanthin
    • 玉米黄质的生产
    • US3951742A
    • 1976-04-20
    • US489473
    • 1974-07-18
    • David ShepherdJaroslav Dasek
    • David ShepherdJaroslav Dasek
    • A23K1/00C12P23/00C12R1/20C12D13/02
    • C12P23/00Y10S435/85
    • A process for the preparation of zeaxanthin which comprises culturing a microorganism of the genus Flavobacter producing this pigment, in a nutrient medium containing at least one carbohydrate as assimilable carbon source, at least one source of assimilable amino nitrogen containing free amino acids, mineral salts, oligoelements and vitamins, the spectrum of amino acids of the medium being modified by increasing the content in the medium of at least one of the sulphur-containing amino acids methionine, cystine and cysteine, in relation to the content in the medium of other amino acids, whilst limiting the quantity of the sulphur-containing amino acid added to the medium to a value not exceeding 1 mg/ml, and continuing culturing until a substantial quantity of intracellular zeaxanthin has been obtained.
    • 一种玉米黄质的制备方法,其包括在含有至少一种碳水化合物作为可同化碳源的营养培养基中培养产生该颜料的黄杆菌属的微生物,至少一种含有游离氨基酸的可同化氨基氮源,无机盐, 寡核苷酸和维生素,相对于其他氨基酸的培养基中的含量,通过增加至少一种含硫氨基酸甲硫氨酸,胱氨酸和半胱氨酸的培养基中培养基中培养基的氨基酸谱, 同时将添加到培养基中的含硫氨基酸的量限制在不超过1mg / ml的值,并继续培养直到获得大量的细胞内玉米黄质。