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1. 发明申请

US20050271629A1 Means for biologically controlling cryptogamic plant diseases 失效
标题翻译：用于生物控制密码植物病害的手段
公开(公告)号：US20050271629A1
公开(公告)日：2005-12-08
申请号：US10520294
申请日：2003-07-01
申请人： Bernard Paul
发明人： Bernard Paul
IPC分类号： A01N63/04 , A01N63/00
CPC分类号： A01N63/04 , Y10S435/837 , Y10S435/838 , Y10S435/839 , Y10S435/874 , Y10S435/88 , Y10S435/938 , Y10S435/945 , A01N63/00 , A01N2300/00
摘要： The invention concerns the application of compositions of micro-organisms in biological control of vin crptogamic diseases. Said composition comprises a mixture of at least one bacterium and at least one yeast, the bacterium or bacteria and the yeast(s) being non-toxic for the plant. The invention also concerns bacterial and yeast strains, as well as biofungicide formulations containing an efficient amount of at least one composition of micro-organisms including in mixture at least one bacterium and one yeast, the bacterium or bacteria and the yeast(s) being non-toxic for the plant, and a composition of filamentous fungi, in particular of the genus Pichia, Pythium, Trichoderma, Gliocladium, Ampelomyces, Talaromyces, Epicococcum, combined with an inert carrier. The invention is useful for treating cryptogamic plant diseases, in particular crop plants and vine.
摘要翻译：本发明涉及微生物组合物在葡萄球菌病的生物学控制中的应用。所述组合物包含至少一种细菌和至少一种酵母，所述细菌或细菌和对植物无毒的酵母的混合物。本发明还涉及细菌和酵母菌株，以及含有有效量的至少一种微生物组合物的生物杀真菌剂，包括至少一种细菌和一种酵母混合物，细菌或细菌和酵母不是对于植物是有毒的，以及丝状真菌的组合物，特别是毕赤酵母属，腐霉属，木霉属，胶霉属，Ampelomyces，Talaromyces，Epicococcum，与惰性载体结合的组合物。本发明可用于治疗隐窝性植物病害，特别是作物植物和葡萄藤。

2. 发明授权

US06329011B1 Antimicrobial agent against acid-resistant and heat-resistant bacteria 失效
标题翻译：耐酸，耐热细菌的抗微生物剂
公开(公告)号：US06329011B1
公开(公告)日：2001-12-11
申请号：US09680403
申请日：2000-10-05
申请人： Shigeru Oita
发明人： Shigeru Oita
IPC分类号： A61K3800
CPC分类号： C07K14/415 , A23L2/02 , A23L2/44 , A61K38/168 , Y10S435/832 , Y10S435/833 , Y10S435/834 , Y10S435/835 , Y10S435/836 , Y10S435/837 , Y10S435/838 , Y10S435/839
摘要： An antimicrobial agent with a high degree of safety is provided, which is derived from a natural product and can exhibit growth-inhibitory activity against acid-resistant and heat-resistant bacteria such as Alicyclobacillus acidoterrestris, which is resistant against pasteurization and causes spoilage of fruit juice. The antimicrobial agent against acid-resistant and heat-resistant bacteria contains as an effective ingredient alpha-type thionin and/or beta-type thionin. A preservative for fruit juice is also provided, which contains as an effective ingredient the alpha-type thionin and/or beta-type thionin.
摘要翻译：提供了具有高度安全性的抗微生物剂，其来自天然产物，并且可以对抗酸碱环境酸杆菌的抗酸耐热细菌表现出抑制生物的活性，其耐巴氏灭菌并引起水果的腐败果汁。针对耐酸耐热细菌的抗微生物剂含有α型硫素和/或β型硫素的有效成分。还提供了果汁防腐剂，其含有α型硫素和/或β型硫素作为有效成分。

3. 发明授权

US5952204A .beta.-fructofuranosidase, its preparation and uses 失效
标题翻译： β-呋喃果糖苷酶，其制备和用途
公开(公告)号：US5952204A
公开(公告)日：1999-09-14
申请号：US941551
申请日：1997-09-30
申请人： Tetsuya Nakada , Hiroto Chaen , Toshiyuki Sugimoto
发明人： Tetsuya Nakada , Hiroto Chaen , Toshiyuki Sugimoto
IPC分类号： C12N9/24 , C12P19/14 , C07H3/04 , C12P19/20
CPC分类号： C12Y302/01026 , C12N9/2431 , Y10S435/832 , Y10S435/837
摘要： A .beta.-fructofuranosidase with a molecular weight of 49,000.+-.5,000 daltons on SDS-PAGE, an isoelectric point of 4.6.+-.0.5, an optimum pH of about 5.5-6.0, and an optimum temperature of about 50.degree. C. in the presence of calcium ion. The enzyme acts on saccharides with a .beta.-fructofuranosidic linkage and other substances including other saccharides, sugar alcohols, and alcohols to produce fructosyl-transferred saccharides in a relatively high yield.
摘要翻译：在SDS-PAGE上的分子量为49,000 +/- 5,000道尔顿的β-呋喃果糖苷酶，等电点为4.6 +/- 0.5，最适pH为约5.5-6.0，最适温度为约50℃ 钙离子的存在。该酶作用于具有β-呋喃果糖苷键的糖类和其它物质，包括其它糖类，糖醇和醇类，以相当高的产率产生果糖基转移的糖类。

4. 发明授权

US4665024A Fluorescent gram stain 失效
标题翻译：荧光革染色
公开(公告)号：US4665024A
公开(公告)日：1987-05-12
申请号：US656627
申请日：1984-10-01
申请人： James D. Mansour
发明人： James D. Mansour
IPC分类号： C12Q1/04 , C12Q1/14 , C12Q1/02 , G01N21/64
CPC分类号： C12Q1/14 , C12Q1/04 , Y10S435/802 , Y10S435/808 , Y10S435/837 , Y10S435/849 , Y10S435/852 , Y10S435/857 , Y10S435/882 , Y10S435/883 , Y10S435/885 , Y10S436/80
摘要： A method to determine the Gram sign of microorganisms includes staining the microorganisms with a plurality of fluorescent dyes, applying excitation energy to the stained microorganisms, and observing the color of the fluorescence emission of the stained microorganisms. Gram-positive and Gram-negative microorganisms stain different colors, and assignment of the Gram sign may be made on the basis of the color of the stained microorganisms.
摘要翻译：确定微生物的革兰氏号的方法包括用多种荧光染料染色微生物，向染色的微生物施加激发能，并观察染色的微生物的荧光发射的颜色。革兰氏阳性和革兰氏阴性微生物染色不同的颜色，并且可以基于染色的微生物的颜色进行赋形。

5. 发明授权

US4427771A Assay method for amylase activity and method of producing maltose dehydrogenase for use therein 失效
标题翻译：淀粉酶活性的测定方法和生产用于其中的麦芽糖脱氢酶的方法
公开(公告)号：US4427771A
公开(公告)日：1984-01-24
申请号：US311263
申请日：1981-10-14
申请人： Hideo Misaki , Eiji Muramatsu , Hidehiko Ishikawa , Kazuo Matsuura
发明人： Hideo Misaki , Eiji Muramatsu , Hidehiko Ishikawa , Kazuo Matsuura
IPC分类号： C12N9/04 , C12Q1/40 , C12N1/20 , C12Q1/32 , C12R1/11
CPC分类号： C12N9/0006 , C12Q1/40 , G01N2400/18 , Y10S435/837
摘要： An assay method for amylase activity in a biological specimen such as serum, saliva or urine. The enzyme amylase in the specimen is used to decompose a substrate which is a glucose polymer having a modified reducing terminal glucose residue or a cyclic glucose polymer. A component of the decomposed substrate is measured as an indication of amylase activity in the specimen. The residue may be amylose, amylopectin, starch, starch hydrolyzate, an etherified reducing terminal, an esterified reducing terminal, gluconolactone or a gluconic acid residue or its derivative. Decomposed substrate assay may be effected by contacting the same with maltose dehydrogenase and NAD or NADP, whereupon the assay is performed by measuring the amount of reduced NAD or reduced NADP, by reacting the same with reduced-form hydrogen transport colorimetric reaction reagent. This reagent may be a tetrazolium salt and diaphorase, or tetrazolium salt and phenazinemethosulfate. To remove pre-existing glucose and maltose present in the specimen, the specimen may be pretreated with alpha-glucosidase or kinase in the presence of Mg.sup.++ and ATP, the kinase being for example hexokinase. The preferred maltose dehydrogenase is produced by culturing Bacillus megaterium B-0779 FERM-P No. 5662.
摘要翻译：生物样品如血清，唾液或尿液中淀粉酶活性的测定方法。试样中的酶淀粉酶用于分解具有修饰的还原性末端葡萄糖残基或环状葡萄糖聚合物的葡萄糖聚合物的底物。测量分解的底物的组分作为样品中淀粉酶活性的指示。残基可以是直链淀粉，支链淀粉，淀粉，淀粉水解物，醚化还原末端，酯化还原末端，葡萄糖酸内酯或葡萄糖酸残基或其衍生物。分解的底物测定可以通过使其与麦芽糖脱氢酶和NAD或NADP接触来实现，因此通过使其与还原形式的氢转运比色反应试剂反应来测量还原的NAD或还原的NADP的量来进行测定。该试剂可以是四唑盐和心律黄素，或四唑鎓盐和吩嗪甲硫酸盐。为了除去样品中存在的现有葡萄糖和麦芽糖，可以在Mg ++和ATP存在下用α-葡糖苷酶或激酶预处理样品，所述激酶例如己糖激酶。优选的麦芽糖脱氢酶是通过培养巨大芽孢杆菌B-0779 FERM-P No.5662产生的。

6. 再颁专利

USRE30872E Process for producing 2-keto-L-gulonic acid 失效
标题翻译： 2-酮-L-古洛糖酸的制备方法
公开(公告)号：USRE30872E
公开(公告)日：1982-02-23
申请号：US114252
申请日：1980-01-22
申请人： Takayasu Sonoyama , Bunji Kageyama , Tahiko Honjo
发明人： Takayasu Sonoyama , Bunji Kageyama , Tahiko Honjo
IPC分类号： C07C59/215 , C07H7/027 , C12P7/60
CPC分类号： C12P7/60 , C07C59/215 , C07H7/027 , Y10S435/83 , Y10S435/837 , Y10S435/84 , Y10S435/859 , Y10S435/874 , Y10S435/883
摘要： 2-Keto-L-gulonic acid is prepared from 2,5-diketo-D-gluconic acid through microbial conversion. The 2-keto-L-gulonic acid producing microorganism used for this microbial conversion includes strains which belong to genera of Brevibacterium, Arthrobacter, Micrococcus, Staphylococcus, Pseudomonas and Bacillus. Both the incubation of the microorganism in a medium containing 2,5-diketo-L-gluconic acid and the direct contact of any products obtained from the cells with a substrate containing said 2,5-diketo-D-gluconic acid may be used in the disclosed process.

7. 发明授权

US4007088A Process of manufacturing native microbial protein with a low content of nucleic acids 失效
标题翻译：制造具有低含量核酸的天然微生物蛋白质的方法
公开(公告)号：US4007088A
公开(公告)日：1977-02-08
申请号：US603153
申请日：1975-08-08
申请人： Zdenek Fencl , Frantisek Machek , Vladimir Sillinger
发明人： Zdenek Fencl , Frantisek Machek , Vladimir Sillinger
IPC分类号： A23J1/00 , A23J1/18 , C12N1/08 , C12D13/06
CPC分类号： C12N1/08 , A23J1/008 , A23J1/18 , Y10S435/837 , Y10S435/849 , Y10S435/858 , Y10S435/911 , Y10S435/921 , Y10S435/93 , Y10S435/938 , Y10S435/944 , Y10S530/82 , Y10S530/821 , Y10S530/825
摘要： The invention relates to a process of manufacturing native microbial protein with a low content of nucleic acids, the product being useful as food or feed. The products obtained by the process of the invention may be widely used in the food industry, especially in the meat, milk, and bread industries. These products may replace meat or milk and can also be used in the nutrition of animals. The products may also be used as substitutes for casein, in the fortification of flour, or to improve the quality and value of bread and the like.
摘要翻译：本发明涉及制备具有低含量核酸的天然微生物蛋白质的方法，该产品可用作食物或饲料。通过本发明的方法获得的产品可广泛用于食品工业，特别是在肉类，牛奶和面包工业中。这些产品可以代替肉或牛奶，也可以用于动物的营养。这些产品也可用作酪蛋白的替代品，在面粉的强化中，或提高面包等的质量和价值。

8. 发明授权

US3888738A Method for purifying cyclodextrin-producing enzymes 失效
标题翻译：净化环糊精生产酶的方法
公开(公告)号：US3888738A
公开(公告)日：1975-06-10
申请号：US41121873
申请日：1973-10-31
申请人： HAYASHIBARA KABUSHIKI KAISHA
发明人： OKADA SHIGETAKA , KITAHATA SUMIO
IPC分类号： C12N9/10 , C07G7/02
CPC分类号： C12N9/1074 , Y10S435/815 , Y10S435/832 , Y10S435/837
摘要： A method for purifying cyclodextrin-producing enzymes, which is characterized in purifying the enzymes by adsorption on starch and/or cyclodextrins.

9. 发明授权

US3821081A Process for producing 7-amino desacetoxy cephalosporanic acid 失效
标题翻译：生产7-氨基甲酸羟丙酸的方法
公开(公告)号：US3821081A
公开(公告)日：1974-06-28
申请号：US23508572
申请日：1972-03-15
申请人： TOYO JOZO KK
发明人： ABE J , MATSUMOTO K , FUJII T , WATANABE T , YAMAGUCHI T
IPC分类号： A61K31/545 , C07D501/18 , C12N9/80 , C12P35/02 , C12R1/11 , C12B1/00
CPC分类号： C12N9/80 , C12P35/02 , Y10S435/815 , Y10S435/837
摘要： D R A W I N G
7-Amino desacetoxy cephalosporanic acid represented by the general formula,

IS PRODUCED ACCORDING TO A PROCESS IN WHICH AN ALKALI METAL SALT OF A 7-ACYLAMINO DESACETOXY CEPHALOSPORANIC ACID REPRESENTED BY THE GENERAL FORMULA,
WHEREIN R is a benzyl or phenoxymethyl group, and M is an alkali metal atom capable of forming a water-soluble salt, is treated with a carrier containing culture filtrate of an enzyme-producing strain belonging to the genus Bacillus which can decompose the amide bond of the said compound, said strain being Bacillus megaterium B-400 FERM-P No. 748, or is treated in an aqueous medium with an enzyme preparation containing said enzymeproducing strain.

10. 发明授权

US3770589A Process for the production of glucose isomerase 失效
标题翻译：生产葡萄糖异构体的方法
公开(公告)号：US3770589A
公开(公告)日：1973-11-06
申请号：US3770589D
申请日：1971-05-20
申请人： CPC INTERNATIONAL INC
发明人： HEADY R , JACAWAY W
IPC分类号： C12N9/92 , C12D13/10
CPC分类号： C12N9/92 , Y10S435/814 , Y10S435/822 , Y10S435/832 , Y10S435/837 , Y10S435/84 , Y10S435/848 , Y10S435/853 , Y10S435/863 , Y10S435/866 , Y10S435/886 , Y10S435/906 , Y10S435/921
摘要： An improved process for the production of glucose isomerase involves the addition of a specific amount of glycine (aminoacetic acid) to the fermentation medium. This increases the amount of the enzyme, glucose isomerase, that is produced by the microorganism, which preferably is a strain of Streptomyces, most preferably, S. olivochromogenes ATCC 21,114. Even further improvement is obtained by also adding a small amount of ammonium nitrate to the culture medium.
摘要翻译：用于生产葡萄糖异构酶的改进方法包括向发酵培养基中加入特定量的甘氨酸（氨基乙酸）。这增加了由微生物产生的酶，葡萄糖异构酶的量，其优选是链霉菌菌株，最优选S.olivochromogenes ATCC 21,114。通过向培养基中加入少量的硝酸铵也能进一步改善。

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