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1. 发明授权

US07626077B2 Glyphosate-resistant plants 有权
标题翻译：抗草甘膦植物
公开(公告)号：US07626077B2
公开(公告)日：2009-12-01
申请号：US11409909
申请日：2006-04-24
申请人： Bruce M. Held , Herbert M. Wilson , Philip E. Dykema , Carol J. Lewnau , Janelle C. Eby
发明人： Bruce M. Held , Herbert M. Wilson , Philip E. Dykema , Carol J. Lewnau , Janelle C. Eby
IPC分类号： C12N15/82
CPC分类号： C12N15/8275
摘要： This invention relates to glyphosate-resistant transgenic plants and methods of making the same. In a preferred embodiment, a DNA fragment which comprises an EPSPS 5′ regulatory sequence and a glyphosate-resistant EPSPS coding sequence is introduced into regenerable plant cells. The encoded EPSPS has a chloroplast transit peptide. The DNA fragment does not contain a non-EPSPS enhancer. Cells are selected for stable transformation, and the selected cells can be used to regenerate glyphosate-resistant transgenic plants. The DNA fragment used for transformation preferably comprises a modified plant genomic sequence, such as SEQ ID NO: 2, SEQ ID NO:4 or SEQ ID NO: 6. In one embodiment, two DNA fragments of this invention are stably transformed into a plant to confer glyphosate-resistance.
摘要翻译：本发明涉及抗草甘膦转基因植物及其制备方法。在优选的实施方案中，将包含EPSPS 5'调节序列和抗草甘磷EPSPS编码序列的DNA片段引入可再生植物细胞中。编码的EPSPS具有叶绿体转运肽。 DNA片段不含非EPSPS增强子。选择细胞用于稳定转化，并且所选择的细胞可用于再生抗草甘膦转基因植物。用于转化的DNA片段优选包含修饰的植物基因组序列，例如SEQ ID NO：2，SEQ ID NO：4或SEQ ID NO：6。在一个实施方案中，本发明的两个DNA片段稳定转化到植物赋予草甘膦抗性。

2. 发明授权

US07045684B1 Glyphosate-resistant plants 有权
公开(公告)号：US07045684B1
公开(公告)日：2006-05-16
申请号：US10223241
申请日：2002-08-19
申请人： Bruce M. Held , Herbert M. Wilson , Philip E. Dykema , Carol J. Lewnau , Janelle C. Eby
发明人： Bruce M. Held , Herbert M. Wilson , Philip E. Dykema , Carol J. Lewnau , Janelle C. Eby
IPC分类号： A01H5/00 , C12N15/82
CPC分类号： C12N15/8275
摘要： This invention relates to glyphosate-resistant transgenic plants and methods of making the same. In a preferred embodiment, a DNA fragment which comprises an EPSPS 5′ regulatory sequence and a glyphosate-resistant EPSPS coding sequence is introduced into regenerable plant cells. The encoded EPSPS has a chloroplast transit peptide. The DNA fragment does not contain a non-EPSPS enhancer. Cells are selected for stable transformation, and the selected cells can be used to regenerate glyphosate-resistant transgenic plants. The DNA fragment used for transformation preferably comprises a modified plant genomic sequence, such as SEQ ID NO: 2, SEQ ID NO:4 or SEQ ID NO: 6. In one embodiment, two DNA fragments of this invention are stably transformed into a plant to confer glyphosate-resistance.

3. 发明授权

US5633363A Root preferential promoter 失效
标题翻译：根优先启动子
公开(公告)号：US5633363A
公开(公告)日：1997-05-27
申请号：US253785
申请日：1994-06-03
申请人： James T. Colbert , Bruce M. Held , Eve S. Wurtele , Paul S. Dietrich
发明人： James T. Colbert , Bruce M. Held , Eve S. Wurtele , Paul S. Dietrich
IPC分类号： C07K14/415 , C12N15/82 , C12N15/11 , A01H5/00 , C12N15/29
CPC分类号： C12N15/8227 , C07K14/415
摘要： The present invention is directed to promoter fragments isolated from maize and particularly to a 4.7 kbp upstream promoter region designated ZRP2 of a ZRP2 genomic DNA clone, specific fragments thereof designated ZRP2(2.0) and ZRF2(1.0) and functional equivalents thereto. The promoter has particular utility in driving root preferential expression of heterologous genes that impart increased agronomic, horticultural and/or pesticidal characteristics to a given plant. This invention is also directed to DNA molecules including said promoters and transformed plant tissue including a DNA molecule comprising a promoter of the invention operably linked to a heterologous gene or genes and the seeds thereof.
摘要翻译：本发明涉及从玉米分离的启动子片段，特别涉及ZRP2基因组DNA克隆的ZRP2的4.7kbp上游启动子区域，其特定片段称为ZRP2（2.0）和ZRF2（1.0）及其功能等同物。启动子在驱动给定植物赋予增加的农艺，园艺和/或杀虫特性的异源基因的根优先表达方面具有特别的用途。本发明还涉及包括所述启动子和转化植物组织的DNA分子，其包括可操作地连接到异源基因或其基因的本发明启动子的DNA分子及其种子。

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