专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20060211004A1 Methods and compositions for determining non-specific cytotoxicity of a transfection agent 审中-公开
公开(公告)号：US20060211004A1
公开(公告)日：2006-09-21
申请号：US11150409
申请日：2005-06-10
申请人： Diane Ilsley , Arindam Bhattacharjee , Emily Anderson , Anastasia Khvorova
发明人： Diane Ilsley , Arindam Bhattacharjee , Emily Anderson , Anastasia Khvorova
IPC分类号： C12Q1/68 , G01N33/53
CPC分类号： C12Q1/6883 , C12Q2600/142 , C12Q2600/158
摘要： Methods and compositions for determining cytotoxicity of a transfection agent are provided. In one aspect, a cell is contacted with a transfection agent. Following contact, a cytotoxic marker profile of the cell is evaluated to determine whether the transfection agent has mediated a cytotoxic response in the cell. Also provided are compositions and reagents, and kits for practicing the subject methods. The subject methods and compositions find use in a variety of different applications.

2. 发明申请

US20060183701A1 Methods and compositions for determining non-specific cytotoxicity of a transfection agent 审中-公开
标题翻译：用于测定转染试剂的非特异性细胞毒性的方法和组合物
公开(公告)号：US20060183701A1
公开(公告)日：2006-08-17
申请号：US11059209
申请日：2005-02-15
申请人： Diane Ilsley , Arindam Bhattacharjee
发明人： Diane Ilsley , Arindam Bhattacharjee
IPC分类号： A61K48/00 , C12Q1/68 , C12N15/63
CPC分类号： C12Q1/6809
摘要： Methods and compositions for determining cytotoxicity of a transfection agent are provided. In one aspect, a cell is contacted with a transfection agent. Following contact, a cytotoxic marker profile of the cell is evaluated to determine whether the transfection agent has mediated a cytotoxic response in the cell. Also provided are compositions and reagents, and kits for practicing the subject methods. The subject methods and compositions find use in a variety of different applications.
摘要翻译：提供了用于确定转染剂的细胞毒性的方法和组合物。在一个方面，使细胞与转染剂接触。接触后，评估细胞的细胞毒性标记物谱，以确定转染试剂是否在细胞中介导细胞毒性反应。还提供了组合物和试剂以及用于实施本发明方法的试剂盒。主题方法和组合物可用于各种不同的应用。

3. 发明申请

US20060121525A1 Methods and compositions for producing linearly amplified amounts of (+) strand RNA 审中-公开
标题翻译：用于产生线性放大量的（+）链RNA的方法和组合物
公开(公告)号：US20060121525A1
公开(公告)日：2006-06-08
申请号：US11341012
申请日：2006-01-27
申请人： Douglas Amorese , Diane Ilsley
发明人： Douglas Amorese , Diane Ilsley
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6865 , C12Q2525/179 , C12Q2525/15
摘要： Methods for producing linearly amplified amounts of (+) strand RNA from an initial mRNA source are provided. In the subject methods, an initial mRNA source, e.g., total RNA, is converted to double-stranded cDNA using a second strand cDNA promoter-primer having a promoter sequence recognized by an RNA polymerase located at its 5′ end. The resultant double-stranded cDNA is then transcribed into (+) RNA. The subject methods find use in a variety of different applications in-which the preparation of linearly amplified amounts of (+) RNA is desired. Also provided are kits for practicing the subject methods.
摘要翻译：提供从初始mRNA来源产生线性扩增量的（+）链RNA的方法。在本发明方法中，使用具有由位于其5'末端的RNA聚合酶识别的启动子序列的第二链cDNA启动子 - 引物将初始mRNA来源（例如总RNA）转化为双链cDNA。然后将所得的双链cDNA转录成（+）RNA。本发明方法可用于各种不同的应用，其中需要制备线性放大量的（+）RNA。还提供了用于实践主题方法的工具包。

4. 发明申请

US20060078899A1 Methods and compositions for reducing label variation in array-based comparative genome hybridization assays 审中-公开
标题翻译：基于阵列的比较基因组杂交测定中减少标记变异的方法和组合物
公开(公告)号：US20060078899A1
公开(公告)日：2006-04-13
申请号：US10964505
申请日：2004-10-12
申请人： Alicia Scheffer , Paige Anderson , Diane Ilsley , Michael Barrett , Bo Curry
发明人： Alicia Scheffer , Paige Anderson , Diane Ilsley , Michael Barrett , Bo Curry
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6841 , C12Q2565/501 , C12Q2525/185
摘要： Methods and compositions for producing a labeled population of nucleic acids for use in an array-based comparative genome hybridization (CGH) assay are provided. In general, the methods involve cleaving a genomic source to produce a sample containing nucleic acid fragments, and end-labeling the nucleic acid fragments to provide a population of nucleic acids having a terminal label. Kits and systems for use in practicing the subject methods are also provided.
摘要翻译：提供了用于生产用于基于阵列的比较基因组杂交（CGH）测定中的标记的核酸群的方法和组合物。通常，所述方法包括切割基因组来源以产生含有核酸片段的样品，并且终止标记核酸片段以提供具有末端标记的核酸群体。还提供了用于实践主题方法的套件和系统。

5. 发明申请

US20050148004A1 Chemical arrays and methods of using the same 失效
标题翻译：化学数组及其使用方法
公开(公告)号：US20050148004A1
公开(公告)日：2005-07-07
申请号：US11008603
申请日：2004-12-08
申请人： Paul Wolber , Robert Kincaid , Douglas Amorese , Diane Ilsley-Tyree , Andrew Atwell , Mel Kronick , Eric Leproust
发明人： Paul Wolber , Robert Kincaid , Douglas Amorese , Diane Ilsley-Tyree , Andrew Atwell , Mel Kronick , Eric Leproust
IPC分类号： B01J19/00 , C12Q1/68 , C40B40/06 , C40B70/00 , C12P19/34
CPC分类号： C12Q1/6883 , B01J2219/00497 , B01J2219/00572 , B01J2219/00574 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00617 , B01J2219/00626 , B01J2219/00659 , B01J2219/00675 , B01J2219/00722 , B82Y30/00 , C12Q1/6806 , C12Q1/6837 , C12Q1/6876 , C12Q2600/158 , C40B40/06 , C40B50/14 , C40B70/00 , C12Q2565/537 , C12Q2525/161 , C12Q2565/525 , C12Q2525/131 , C12Q2521/301
摘要： Methods and compositions for generating mixtures of product molecules from an initial chemical array are provided. In the subject methods, a chemical array of surface immobilized first moieties is subjected to cleavage conditions such that a composition of solution phase first moieties is produced. The resultant composition of solution phase first moieties is then contacted with one or more reactants to produce a mixture of product molecules that are different from the first moieties. Also provided are the arrays employed in the subject methods and kits for practicing the subject methods.
摘要翻译：提供了从初始化学阵列产生产物分子的混合物的方法和组合物。在本发明方法中，表面固定的第一部分的化学阵列经受切割条件，使得产生溶液相第一部分的组合物。然后将所得到的溶液相第一部分的组合物与一种或多种反应物接触以产生不同于第一部分的产物分子的混合物。还提供了用于实践本发明方法的主题方法和试剂盒中使用的阵列。

6. 发明申请

US20060223094A1 Methods and compositions for producing labeled probe nucleic acids for use in array based comparative genomic hybridization applications 审中-公开
标题翻译：用于生产标记探针核酸的方法和组合物，用于基于阵列的比较基因组杂交应用
公开(公告)号：US20060223094A1
公开(公告)日：2006-10-05
申请号：US11376727
申请日：2006-03-14
申请人： Douglas Amorese , Diane Ilsley
发明人： Douglas Amorese , Diane Ilsley
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q2521/101 , C12Q2563/107 , C12Q2565/501
摘要： Methods and compositions for producing labeled probe nucleic acids from genomic nucleic acid template are provided. In the subject methods, a conserved coding consensus region primer is employed to enzymatically generate a select set of labeled probe nucleic acids corresponding to coding regions of genes from a genomic template via a primer extension protocol. The subject methods find use in a variety of different applications, and are particularly suited for use in the preparation of labeled probe nucleic acids for use in array based comparative genomic hybridization applications. Also provided are kits for use in practicing the subject methods.
摘要翻译：提供了用于从基因组核酸模板生产标记的探针核酸的方法和组合物。在本发明方法中，采用保守编码共有区引物，通过引物延伸方案酶法产生对应于来自基因组模板的基因编码区的标准探针核酸选择集。本发明方法可用于各种不同的应用，并且特别适用于制备用于基于阵列的比较基因组杂交应用中的标记的探针核酸。还提供了用于实践主题方法的试剂盒。

7. 发明申请

US20050136411A1 Methods and compositions for linear mRNA amplification from small RNA samples 审中-公开
标题翻译：来自小RNA样品的线性mRNA扩增的方法和组合物
公开(公告)号：US20050136411A1
公开(公告)日：2005-06-23
申请号：US10744434
申请日：2003-12-22
申请人： Patrick Collins , Khanh Nguyen , Diane Ilsley
发明人： Patrick Collins , Khanh Nguyen , Diane Ilsley
IPC分类号： C12Q1/68 , C12P19/34 , G01N33/48 , G01N33/50 , G06F19/00
CPC分类号： C12Q1/6809 , C12Q1/6865 , C12Q2531/101 , C12Q2527/125 , C12Q2525/143
摘要： Methods and compositions for linearly amplifying mRNA from small RNA samples to produce antisense RNA are provided In the subject methods, mRNA is converted to a transcription template product by using a promoter primer, where the resultant product at least includes a first strand cDNA template domain and a double-stranded RNA polymerase promoter. A feature of the subject methods is that the ratio of the promoter-primer to the input RNA is chosen to provide for a high yield of selective RNA product. Additional optional features include the use of a thermostabilizing agent, e.g., raffinose, trehalose, etc., in the reverse transcription step and/or the use of a polyalkylene oxide, e.g., a PEG, in the in vitro transcription step. Also provided are compositions and kits that find use in practicing the subject methods. The subject invention finds use a variety of different applications in which the preparation of linearly amplified amounts of cRNA is desired.
摘要翻译：提供从小RNA样品线性扩增mRNA以产生反义RNA的方法和组合物在本主题方法中，通过使用启动子引物将mRNA转化为转录模板产物，其中所得产物至少包含第一链cDNA模板结构域和双链RNA聚合酶启动子。本发明方法的特征是选择启动子 - 引物与输入RNA的比例以提供高产率的选择性RNA产物。另外的任选特征包括在逆转录步骤中使用热稳定剂，例如棉子糖，海藻糖等，和/或在体外转录步骤中使用聚环氧烷，例如PEG。还提供了用于实践主题方法的组合物和试剂盒。本发明发现使用其中需要制备线性扩增量的cRNA的多种不同应用。

8. 发明授权

US07588889B2 Chemical arrays and methods of using the same 失效
标题翻译：化学数组及其使用方法
公开(公告)号：US07588889B2
公开(公告)日：2009-09-15
申请号：US11008603
申请日：2004-12-08
申请人： Paul K. Wolber , Robert H. Kincaid , Douglas Amorese , Diane Ilsley-Tyree , Andrew S. Atwell , Mel N. Kronick , Eric M. Leproust
发明人： Paul K. Wolber , Robert H. Kincaid , Douglas Amorese , Diane Ilsley-Tyree , Andrew S. Atwell , Mel N. Kronick , Eric M. Leproust
IPC分类号： C12Q1/68 , C12M1/00 , C07H21/04
CPC分类号： C12Q1/6883 , B01J2219/00497 , B01J2219/00572 , B01J2219/00574 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00617 , B01J2219/00626 , B01J2219/00659 , B01J2219/00675 , B01J2219/00722 , B82Y30/00 , C12Q1/6806 , C12Q1/6837 , C12Q1/6876 , C12Q2600/158 , C40B40/06 , C40B50/14 , C40B70/00 , C12Q2565/537 , C12Q2525/161 , C12Q2565/525 , C12Q2525/131 , C12Q2521/301
摘要： Methods and compositions for generating mixtures of product molecules from an initial chemical array are provided. In the subject methods, a chemical array of surface immobilized first moieties is subjected to cleavage conditions such that a composition of solution phase first moieties is produced. The resultant composition of solution phase first moieties is then contacted with one or more reactants to produce a mixture of product molecules that are different from the first moieties. Also provided are the arrays employed in the subject methods and kits for practicing the subject methods.
摘要翻译：提供了从初始化学阵列产生产物分子的混合物的方法和组合物。在本发明方法中，表面固定的第一部分的化学阵列经受切割条件，使得产生溶液相第一部分的组合物。然后将所得到的溶液相第一部分的组合物与一种或多种反应物接触以产生不同于第一部分的产物分子的混合物。还提供了用于实践本发明方法的主题方法和试剂盒中使用的阵列。

9. 发明申请

US20070111218A1 Label integrity verification of chemical array data 审中-公开
标题翻译：化学阵列数据的标签完整性验证
公开(公告)号：US20070111218A1
公开(公告)日：2007-05-17
申请号：US11282527
申请日：2005-11-17
申请人： Diane Ilsley , James Minor
发明人： Diane Ilsley , James Minor
IPC分类号： C12Q1/68 , G06F19/00
CPC分类号： G01N33/582 , C12Q1/6837 , G01N33/58 , C12Q2565/102 , C12Q2545/101
摘要： Methods, systems and computer readable media for checking label integrity of labeled biopolymers in a single sample assayed by chemical array analysis. At least first and second labels are incorporated into biopolymers in the single sample to produce a multi-labeled, single sample. The multi-labeled, single sample is hybridized with probes on a chemical array, and signal values are read from probes on the chemical array bound to a set of biopolymer sequences labeled with the at least first and second labels. First-labeled signal values from a probe, having the first label incorporated therein, are compared with second-labeled signal values from the probe bound to biopolymer having the second label incorporated therein. The reading signal values and comparing steps are repeated for at least one additional probe on the chemical microarray bound to a set of different biopolymer sequences labeled with the at t least first and second labels, and label integrity is determined to be of acceptable quality if divergence between the first-labeled signal values read from the probes and the second-labeled signal values read from the same probes is less than a predetermined threshold value.
摘要翻译：用于通过化学阵列分析测定的单个样品中标记的生物聚合物的标签完整性的方法，系统和计算机可读介质。至少第一和第二标签被并入单个样品中的生物聚合物中以产生多标记的单个样品。多标记的单个样品与化学阵列上的探针杂交，并且从与至少第一和第二标记标记的一组生物聚合物序列结合的化学阵列上的探针读取信号值。将具有掺入其中的第一标记的探针的第一标记信号值与来自结合到其中结合有第二标签的生物聚合物的探针的第二标记信号值进行比较。对于与至少第一和第二标签标记的一组不同生物聚合物序列结合的化学微阵列上的至少一个附加探针重复阅读信号值和比较步骤，并且如果发散发生，标签完整性被确定为具有可接受的质量在从探针读取的第一标记信号值和从相同探针读取的第二标记信号值之间小于预定阈值。

10. 发明申请

US20070092869A1 Spike-in controls and methods for using the same 审中-公开
标题翻译： Spike-in控件和使用它们的方法
公开(公告)号：US20070092869A1
公开(公告)日：2007-04-26
申请号：US11580675
申请日：2006-10-13
申请人： Stephanie Fulmer-Smentek , Anne Lucas , Diane Ilsley
发明人： Stephanie Fulmer-Smentek , Anne Lucas , Diane Ilsley
IPC分类号： C12Q1/70 , C12Q1/68 , G06F19/00
CPC分类号： C12Q1/6837 , G16B25/00 , C12Q2565/513
摘要： Methods, compositions, and kits for performing a one-color analysis of microarray data are provided. Also disclosed are compositions including control nucleic acid sequences, and methods for measuring the dynamic range of a microarray analysis.
摘要翻译：提供了用于进行微阵列数据的单色分析的方法，组合物和试剂盒。还公开了包含对照核酸序列的组合物和用于测量微阵列分析的动态范围的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式