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1. 发明授权

US10786800B2 Methods and systems for epi-fluorescent monitoring and scanning for microfluidic assays 有权
公开(公告)号：US10786800B2
公开(公告)日：2020-09-29
申请号：US15477902
申请日：2017-04-03
申请人： CyVek, Inc.
发明人： Martin A. Putnam , Jeffrey T. Branciforte , Charles O. Stanwood
IPC分类号： G01N21/05 , B01J19/00 , G01N35/00 , G01N21/64 , B01L3/00 , G01N33/543 , G01N21/03
摘要： A method and system for heating and/or inspecting a portable microfluidic assay cartridge for performing an assay includes receiving the assay cartridge on a receiving region of a translatable table under automated control, heating the cartridge, during performance of the assay, with a planar radiant heater plate, the heater plate having an aperture through which an inspection axis extends, and/or inspecting the cartridge using an optical system constructed to inspect the cartridge along the inspection axis by reading a fluorescent light signal which passes through the aperture in the heater plate. In addition, the cartridge moves with movement of the translation table, and the heater plate and optical system may be stationary, and the inspection axis may be fixed.

2. 发明申请

US20200047476A1 Microfluidic Assay Assemblies and Methods of Manufacture 审中-公开
公开(公告)号：US20200047476A1
公开(公告)日：2020-02-13
申请号：US16570127
申请日：2019-09-13
申请人： CyVek, Inc.
发明人： Martin A. Putnam
IPC分类号： B32B37/10 , B32B37/00 , B32B37/02 , B01L3/00 , G01N21/05 , G01N21/64 , G01N33/543
摘要： A method of making at least a portion of at least one microfluidic actuator having a flexible diaphragm portion and an opposite surface portion, the diaphragm and opposite surface each having opposed faces, at least one of the faces comprising surface-activated PDMS, and the opposed faces being arranged such that when the opposed faces contact each other, they form a fluidic seal, including performing repeated make-and-break-contact protocol on the contacting opposed faces until the tendency for permanent bonds to form between the contacting faces has been neutralized, thereby enabling the diaphragm portion to perform actuated movements to engage and disengage with the opposite surface portion, without the diaphragm sticking to the opposite surface portion.

3. 发明申请

US20170297022A1 Methods and Systems for Manufacture of Microarray Assay Systems, Conducting Microfluidic Assays, and Monitoring and Scanning to Obtain Microfluidic Assay Results 审中-公开
公开(公告)号：US20170297022A1
公开(公告)日：2017-10-19
申请号：US15581526
申请日：2017-04-28
申请人： CyVek, Inc.
发明人： Martin A. Putnam , Jeffrey T. Branciforte , Charles O. Stanwood
IPC分类号： B01L3/00 , G01N33/543 , G01N21/64
CPC分类号： B01L3/502715 , B01L3/502707 , B01L3/50273 , B01L9/527 , B01L2200/027 , B01L2200/0689 , B01L2200/148 , B01L2200/16 , B01L2300/0636 , B01L2300/0819 , B01L2300/0838 , B01L2300/0864 , B01L2300/087 , B01L2300/0887 , B01L2300/168 , B01L2400/0481 , B01L2400/0638 , B01L2400/0655 , B01L2400/086 , G01N21/05 , G01N21/6428 , G01N21/645 , G01N33/54366 , G01N35/00712 , G01N2021/0346 , G01N2021/058 , G01N2021/6439 , G01N2035/00158 , Y10T29/494 , Y10T436/10
摘要： A method of flowing a fluid with a tracer in a microfluidic channel of an assay device and detecting the tracer for determining the channel location or condition of the channel.

4. 发明申请

US20150083313A1 Portable Microfluidic Assay Devices and Methods of Manufacture and Use 有权
标题翻译：便携式微流控测定装置及其制造和使用方法
公开(公告)号：US20150083313A1
公开(公告)日：2015-03-26
申请号：US14479287
申请日：2014-09-06
申请人： CyVek, Inc.
发明人： Martin A. Putnam
IPC分类号： G01N33/53 , B32B37/02 , B32B38/00 , B32B37/00
CPC分类号： B32B38/0004 , B01L3/502707 , B01L2200/0689 , B01L2200/16 , B01L2300/087 , B01L2300/0887 , B01L2300/168 , B01L2400/0481 , B01L2400/0638 , B01L2400/086 , B32B37/0076 , B32B37/02 , G01N21/05 , G01N21/645 , G01N33/54366 , G01N2021/0346 , G01N2021/058 , G01N2035/00158 , Y10T29/494 , Y10T156/10 , Y10T156/1052
摘要： A method of forming a pneumatically controlled microfluidic device containing a micro-fluidic network that includes micro-elements in the form of micro-particles or micro-length hollow flow elements, and a pneumatic network that includes pneumatic micro-channels and micro-valve features enabling membrane valves to operate to control fluid conditions in the microfluidic network, including the steps (a) forming two portions, denominated fluidic layer, in which microfluidic channels are formed, and pneumatic layer, in which pneumatic micro-channels and micro-valve features are formed, each having a backing that is rigid in the plane of extent of the layers, (b) providing an intervening elastic membrane, and (c) permanently bonding both layers to opposite sides of the membrane, the permanent bonding of the membrane to the fluidic layer being effective to permanently enclose a set of inserted micro-elements in the fluidic network and relate the two layers to enable pneumatic control of fluid conditions in the microfluidic network.
摘要翻译：一种形成包含微流体网络的气动微流体装置的方法，微流体网络包括微粒或微长度中空流动元件形式的微元件，以及包括气动微通道和微阀特征的气动网络使得膜阀能够操作以控制微流体网络中的流体状况，包括步骤（a）形成两个部分，其中形成有微流体通道的流体层和气动层，气动微通道和微阀特征形成，每个具有在层的平面中刚性的背衬，（b）提供中间弹性膜，和（c）将两层永久地粘合到膜的相对侧，将膜永久粘合到流体层有效地将一组插入的微元件永久地包围在流体网络中并且使两个层相关联以使得能够对流体进行气动控制 d微流控网络中的条件。

5. 发明授权

US10414143B2 Microfluidic assay assemblies and methods of manufacture 有权
公开(公告)号：US10414143B2
公开(公告)日：2019-09-17
申请号：US16118985
申请日：2018-08-31
申请人： CyVek, Inc.
发明人： Martin A. Putnam
IPC分类号： B32B37/10 , B32B37/00 , B32B37/02 , B01L3/00 , G01N21/05 , G01N21/64 , G01N33/543 , G01N21/03 , G01N35/00
摘要： A method of making at least a portion of at least one microfluidic actuator having a flexible diaphragm portion and an opposite surface portion, the diaphragm and opposite surface each having opposed faces, at least one of the faces comprising surface-activated PDMS, and the opposed faces being arranged such that when the opposed faces contact each other, they form a fluidic seal, including performing repeated make-and-break-contact protocol on the contacting opposed faces until the tendency for permanent bonds to form between the contacting faces has been neutralized, thereby enabling the diaphragm portion to perform actuated movements to engage and disengage with the opposite surface portion, without the diaphragm sticking to the opposite surface portion.

6. 发明授权

US10252263B2 Microfluidic devices and methods of manufacture and use 有权
公开(公告)号：US10252263B2
公开(公告)日：2019-04-09
申请号：US14955785
申请日：2015-12-01
申请人： CyVek, Inc.
发明人： Martin A. Putnam , Jeffrey T. Branciforte , Charles O. Stanwood
IPC分类号： B29C65/00 , B29C65/02 , B32B37/00 , B32B38/00 , B65C3/26 , B32B38/04 , B32B27/00 , G01N33/00 , G01N15/06 , G01N33/48 , B01L3/00 , C12M1/34 , C12M3/00 , F15C1/06 , G01N21/05 , G01N21/64 , G01N33/543 , B32B37/14 , B32B37/18 , G01N21/03
摘要： Microfluidic devices are provided for conducting fluid assays, for example biological assays, that have the ability to move fluids through multiple channels and pathways in a compact, efficient, and low cost manner. Discrete flow detection elements, preferably extremely short hollow flow elements, with length preferably less than 700 micron, preferably less than 500 micron, and internal diameter preferably of between about 50+/−25 micron, are provided with capture agent, and are inserted into microfluidic channels by tweezer or vacuum pick-and-place motions at fixed positions in which they are efficiently exposed to fluids for conducting assays. Close-field electrostatic attraction is employed to define the position of the elements and enable ready withdrawal of the placing instruments. The microfluidic devices feature flow elements, channels, valves, and on-board pumps that are low cost to fabricate accurately, are minimally invasive to the fluid path and when implemented for the purpose, can produce multiplex assays on a single portable assay cartridge (chip) that have low coefficients of variation. Novel methods of construction, assembly and use of these features are presented, including co-valent bonding of selected regions of faces of surface-activatable bondable materials, such as PDMS to PDMS and PDMS to glass, while contiguous portions of one flexible sheet completes and seals flow channels, fixes the position of inserted analyte-detection elements in the channels, especially short hollow flow elements through which sample and reagent flow, and other portions form flexible valve membranes and diaphragms of pumps. A repeated make-and-break-contact manufacturing protocol prevents such bonding to interfere with moving the integral valve diaphragm portions from their valve seats defined by the opposed sheet member, which the flexible sheet material engages. Preparation of two subassemblies, each having a backing of relatively rigid material, followed by their assembly face-to-face in a permanent bond is shown. Hollow detection flow elements are shown fixed in channels, that provide by-pass flow paths of at least 50% of the flow capacity through the elements; in preferred implementations, as much as 100% or more. Metallized polyester film is shown to have numerous configurations and advantages in non-permanently bonded constructions. A method of preparing detection elements for an assay comprises batch coating detection elements, or hollow flow elements by mixing and picking and placing the elements in flow channels of a microfluidic device, capturing the flow elements by bonding two opposed layers while sealing the flow channels.

7. 发明申请

US20150086424A1 Micro-Tube Particles for Microfluidic Assays and Methods of Manufacture 有权
标题翻译：用于微流控测定的微管颗粒和制造方法
公开(公告)号：US20150086424A1
公开(公告)日：2015-03-26
申请号：US14479283
申请日：2014-09-06
申请人： CyVek, Inc.
发明人： Martin A. Putnam , Jeffrey T. Branciforte , Charles O. Stanwood , Jane M. Tu
IPC分类号： G01N33/543 , B01L3/00
CPC分类号： B01L3/502707 , B01L3/502715 , B01L3/502761 , B01L2200/0689 , B01L2200/12 , B01L2200/16 , B01L2300/0832 , B01L2300/0838 , B01L2300/0864 , B01L2300/087 , B01L2300/0887 , B01L2300/0896 , B01L2300/12 , B01L2300/123 , B01L2300/168 , B01L2400/0481 , B01L2400/0638 , B01L2400/0655 , B01L2400/086 , G01N21/05 , G01N21/645 , G01N33/54366 , G01N33/54386 , G01N2021/0346 , G01N2021/058 , G01N2035/00158 , Y10T29/494
摘要： A microfluidic assay device that defines a micro-fluidic flow channel (44) having a flow axis, in which a series of discrete, axially-spaced apart, transparent hollow flow elements (32) are secured in fixed position, each flow element having at least one axially-extending flow passage through its interior, assay capture agent fixed to the interior surface of the elements for capture of an analyte in liquid flowing through the interior of the flow elements, the device constructed to enable light to be transmitted out of the elements for reading of fluorescence from captured analyte, wherein: the exterior axially-extending surfaces of the flow elements are free of active capture agent, while at least part of the interior surfaces carry deposits of active capture agent exposed to flow through the elements.
摘要翻译：一种限定具有流动轴线的微流体流动通道（44）的微流体测定装置，其中一系列离散的，轴向间隔开的透明中空流动元件（32）固定在固定位置，每个流动元件具有至少一个轴向延伸的通过其内部的流动通道，测定捕获剂固定到元件的内表面，用于捕获流过流动元件内部的液体中的分析物，该装置构造成使得光能够从用于从捕获的分析物读取荧光的元件，其中：流动元件的外部轴向延伸的表面没有活性捕获剂，而至少部分内表面携带暴露于流过元件的活性捕获剂的沉积物。

8. 发明申请

US20140377852A1 Methods and Systems for Epi-Fluorescent Monitoring and Scanning for Microfluidic Assays 有权
标题翻译：用于微流控测定的荧光显微镜监测和扫描的方法和系统
公开(公告)号：US20140377852A1
公开(公告)日：2014-12-25
申请号：US14479290
申请日：2014-09-06
申请人： CyVek, Inc.
发明人： Martin A. Putnam , Jeffrey T. Branciforte , Charles O. Stanwood
IPC分类号： G01N35/00
CPC分类号： B01J19/0046 , B01J2219/00286 , B01J2219/00468 , B01J2219/005 , B01J2219/00511 , B01J2219/00522 , B01J2219/00585 , B01J2219/00587 , B01J2219/00596 , B01J2219/00689 , B01J2219/00691 , B01L3/502707 , B01L3/502715 , B01L3/502738 , B01L2200/021 , B01L2200/025 , B01L2200/027 , B01L2200/028 , B01L2200/0631 , B01L2200/0689 , B01L2200/16 , B01L2300/0636 , B01L2300/0816 , B01L2300/0819 , B01L2300/0838 , B01L2300/0864 , B01L2300/087 , B01L2300/0874 , B01L2300/0887 , B01L2300/123 , B01L2300/168 , B01L2300/1805 , B01L2400/0481 , B01L2400/0487 , B01L2400/049 , B01L2400/0638 , B01L2400/0655 , B01L2400/086 , G01N21/05 , G01N21/645 , G01N21/6458 , G01N33/54366 , G01N35/00029 , G01N2021/0346 , G01N2021/058 , G01N2035/00039 , G01N2035/00108 , G01N2035/00158 , G01N2035/00752 , Y10T29/494
摘要： An operating and reading instrument for performing an assay employing a portable microfluidic assay cartridge, the instrument comprising a translatable table under automated control, the translatable table carrying a receiving region for the portable cartridge and carrying a port system connectible to the cartridge that includes at least one remotely automated valve carried by the translatable table, the valve arranged to apply pressurized flowable substance at selected times to the cartridge while the cartridge is on the translatable table.
摘要翻译：一种用于使用便携式微流控测定盒执行测定的操作和阅读仪器，所述仪器包括在自动控制下的可翻译工作台，所述可移动工作台承载用于所述便携式存储器的接收区域，并且携带可连接到所述盒的端口系统，所述端口系统至少包括一个远程自动化的阀，由可移动工作台承载，该阀设置成在该盒位于可平移台上时，将选定的时间加压的可流动物质施加到该盒。

9. 发明授权

US11292237B2 Microfluidic assay assemblies and methods of manufacture 有权
公开(公告)号：US11292237B2
公开(公告)日：2022-04-05
申请号：US16570127
申请日：2019-09-13
申请人： CyVek, Inc.
发明人： Martin A. Putnam
IPC分类号： B32B37/10 , B32B37/00 , B32B37/02 , B01L3/00 , G01N21/05 , G01N21/64 , G01N33/543 , G01N21/03 , G01N35/00
摘要： A method of making at least a portion of at least one microfluidic actuator having a flexible diaphragm portion and an opposite surface portion, the diaphragm and opposite surface each having opposed faces, at least one of the faces comprising surface-activated PDMS, and the opposed faces being arranged such that when the opposed faces contact each other, they form a fluidic seal, including performing repeated make-and-break-contact protocol on the contacting opposed faces until the tendency for permanent bonds to form between the contacting faces has been neutralized, thereby enabling the diaphragm portion to perform actuated movements to engage and disengage with the opposite surface portion, without the diaphragm sticking to the opposite surface portion.

10. 发明授权

US10209250B2 PDMS membrane-confined nucleic acid and antibody/antigen-functionalized microlength tube capture elements, and systems employing them, and methods of their use 有权
公开(公告)号：US10209250B2
公开(公告)日：2019-02-19
申请号：US15638526
申请日：2017-06-30
申请人： CyVek, Inc.
发明人： Martin A. Putnam , John H. Leamon , Jeffrey T. Branciforte , Charles O. Stanwood
IPC分类号： G01N33/543 , B01L3/00 , G01N21/05 , G01N21/64 , G01N21/03 , G01N35/00
摘要： A method for performing a combined protein and nucleic acid assay on a target captured by a capture agent, includes providing a microfluidic device having a microfluidic channel network having at least one microfluidic channel, the channel arranged to receive fluid, the device having at least two micro-particles disposed in fixed position in the channel, the micro-particles being functionalized with a capture agent for the assay, one of the micro-particles in the channel being functionalized with an antibody or antigen capture agent and another of the micro-particles being functionalized with a nucleic acid capture agent. In some embodiments, the network may have at least two microfluidic channels, each channel of the two channels arranged to receive portions of the same fluid and to be fluidicly isolatable from each other, the device having at least two micro-particles disposed in fixed position in the network channels, the micro-particles being functionalized with a capture agent, one of the micro-particles in one of the channels being functionalized with an antibody or antigen capture agent and another of the micro-particles in another of the channels being functionalized with a nucleic acid capture agent. The method may also include detecting both protein and nucleic acid present in an input sample using the respectively functionalized micro particles. In some embodiments, the micro particles may be micro-length tubes or glass nano reactors.

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