专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US07172887B2 Methods for producing citric acid using host cells deficient in oxaloacetate hydrolase 有权
标题翻译：使用缺乏草酰乙酸水解酶的宿主细胞产生柠檬酸的方法
公开(公告)号：US07172887B2
公开(公告)日：2007-02-06
申请号：US11122506
申请日：2005-05-04
申请人： Carsten Mailand Hjort , Henrik Pedersen
发明人： Carsten Mailand Hjort , Henrik Pedersen
IPC分类号： C12P7/48 , C12N15/63 , C12N1/15
CPC分类号： C12Q1/6895 , C12N9/14
摘要： The present invention relates to isolated nucleic acid sequences encoding polypeptides having oxaloacetate hydrolase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as recombinant methods for producing the polypeptides.
摘要翻译：本发明涉及编码具有草酰乙酸水解酶活性的多肽的分离的核酸序列。本发明还涉及包含核酸序列的核酸构建体，载体和宿主细胞以及用于产生多肽的重组方法。

2. 发明授权

US06936438B2 Method of producing a polypeptide using oxaloacetate hydrolase deficient fungal host cells 有权
标题翻译：使用草酰乙酸水解酶缺陷型真菌宿主细胞产生多肽的方法
公开(公告)号：US06936438B2
公开(公告)日：2005-08-30
申请号：US10336491
申请日：2003-01-03
申请人： Carsten Mailand Hjort , Henrik Pedersen
发明人： Carsten Mailand Hjort , Henrik Pedersen
IPC分类号： C12N1/21 , C12N9/14 , C12N15/55 , C12N21/06 , C12N9/10 , C12N15/63
CPC分类号： C12Q1/6895 , C12N9/14
摘要： The present invention relates to methods of producing a polypeptide comprising culturing a mutant of a parent cell, wherein the mutant produces less oxaloacetate hydrolase than the parent cell, wherein the oxaloacetate hydrolase (i) has an amino acid sequence that has at least 90% identity with amino acids 1-341 of SEQ ID NO: 2: (ii) is encoded by a nucleic acid sequence having at least 90% homology with a nucleotide sequence comprising nuclotides 1157-1411, 1504-1651 and 1764-2383 of SEQ ID NO: 1: (iii) is encoded by a nucleic acid sequence which hybridizes under high stringency conditions with the nucleic acid sequence of SEQ ID NO: 1, the cDNA sequence of SEQ ID NO: 1, the complementary strand of SEQ ID NO: 1, and/or the complementary strand of the cDNA sequence of SEQ ID NO: 1: and/or (iv) a subsequence of one or both of (i) and (ii), wherein the subsequence encodes a fragment that has oxaloacetate hydrolase activity.
摘要翻译：本发明涉及生产多肽的方法，其包括培养亲本细胞的突变体，其中所述突变体比母体细胞产生较少的草酰乙酸水解酶，其中草酰乙酸水解酶（i）具有至少90％同一性的氨基酸序列与SEQ ID NO：2的氨基酸1-341：（ii）由与SEQ ID NO的核苷酸1157-1411,1504-1651和1764-2383的核苷酸序列具有至少90％同源性的核酸序列编码 1：（iii）由高度严格条件下与SEQ ID NO：1的核酸序列，SEQ ID NO：1的cDNA序列，SEQ ID NO：1的互补链杂交的核酸序列编码，和/或SEQ ID NO：1的cDNA序列的互补链和/或（iv）（i）和（ii）中的一个或两个的亚序列，其中所述亚序列编码具有草酰乙酸水解酶活性的片段。

3. 发明授权

US06346244B1 Fungal protein disulfide isomerase 失效
标题翻译：真菌蛋白二硫键异构酶
公开(公告)号：US06346244B1
公开(公告)日：2002-02-12
申请号：US09262666
申请日：1999-03-04
申请人： Carsten Mailand Hjort
发明人： Carsten Mailand Hjort
IPC分类号： A61K3843
CPC分类号： D06M16/003 , A21D8/042 , A23K10/14 , A23L29/06 , A61K8/66 , A61K38/00 , A61Q5/00 , C12N9/90 , C12Y503/04001
摘要： The present invention relates to protein disulfide isomerases which are encoded by a nucleic acid sequence which hybridizes with (i) the DNA sequence of SEQ ID NO:1 or (ii) the DNA sequence of SEQ ID NO:2, under the following conditions: presoaking in 5×SSC and prehybridizing for 1 h at ˜40° C. in a solution of 5×SSC, 5×Denhardt's solution, 50 mM sodium phosphate, pH 6.8, and 50 &mgr;g of denatured sonicated calf thymus DNA, followed by hybridization in the same solution supplemented with 50 &mgr;Ci 32-P-dCTP labelled probe for 18 h at ˜40° C. followed by washing three times in 2×SSC, 0.2% SDS at 40° C. for 30 minutes; and fragments thereof. The present invention also relates to DNA sequences encoding the protein disulfide isomerases, compositions comprising said protein disulfide isomerases and methods of use thereof.
摘要翻译：本发明涉及由以下条件与（i）SEQ ID NO：1的DNA序列或（ii）SEQ ID NO：2的DNA序列杂交的核酸序列编码的蛋白质二硫键异构酶：在5xSSC中预浸泡，并在约40℃下在5xSSC，5xDenhardt溶液，50mM磷酸钠，pH6.8和50ml变性超声小牛胸腺DNA的溶液中预杂交1小时，然后在补充有 50μC32-P-dCTP标记的探针在〜40℃下培养18小时，然后在2xSSC，0.2％SDS中在40℃下洗涤3次30分钟; 及其片段。本发明还涉及编码蛋白质二硫键异构酶的DNA序列，包含所述蛋白质二硫键异构酶的组合物及其使用方法。

4. 发明授权

US07919275B2 Oxaloacetate hydrolase deficient fungal host cells 有权
标题翻译：草酰乙酸水解酶缺陷型真菌宿主细胞
公开(公告)号：US07919275B2
公开(公告)日：2011-04-05
申请号：US12719648
申请日：2010-03-08
申请人： Carsten Mailand Hjort , Henrik Pedersen , Jens Nielsen
发明人： Carsten Mailand Hjort , Henrik Pedersen , Jens Nielsen
IPC分类号： C12N1/15 , C12P21/00
CPC分类号： C12Q1/6895 , C12N9/14
摘要： An isolated mutant host cell produced by disrupting or deleting, in a parent cell, a nucleic acid sequence encoding an oxaloacetate hydrolase, or a promoter thereof, which results in the mutant cell producing less of the oxaloacetate hydrolase than the parent cell is disclosed. The disclosure also relates to recombinant methods for producing the products such as polypeptides.
摘要翻译：公开了一种分离的突变宿主细胞，其通过在母体细胞中破坏或缺失编码草酰乙酸水解酶的核酸序列或其启动子，导致突变体细胞产生比母细胞少的草酰乙酸酯水解酶。本公开还涉及用于生产诸如多肽的产物的重组方法。

5. 发明授权

US06544765B1 Oxaloacetate hydrolase deficient fungal host cells 有权
公开(公告)号：US06544765B1
公开(公告)日：2003-04-08
申请号：US09501612
申请日：2000-02-10
申请人： Carsten Mailand Hjort , Henrik Pedersen
发明人： Carsten Mailand Hjort , Henrik Pedersen
IPC分类号： C12N914
CPC分类号： C12Q1/6895 , C12N9/14
摘要： The present invention relates to isolated nucleic acid sequences encoding polypeptides having oxaloacetate hydrolase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as recombinant methods for producing the polypeptides.

6. 发明授权

US06423524B1 Cellulase preparation comprising an endoglucanase enzyme 失效
标题翻译：包含内切葡聚糖酶的纤维素酶制剂
公开(公告)号：US06423524B1
公开(公告)日：2002-07-23
申请号：US09189028
申请日：1998-11-10
申请人： Fred Hagen , Carsten Mailand Hjort , Sven Hastrup
发明人： Fred Hagen , Carsten Mailand Hjort , Sven Hastrup
IPC分类号： C12N1500
CPC分类号： C12Q1/6895 , C11D3/38645 , C12N9/2437 , C12N15/80 , C12Y302/01004 , C12Y302/01039 , C12Y302/01091 , D06M16/003 , D06P5/137 , D06P5/158 , D21H17/005 , D21H21/10
摘要： The present invention relates to cellulase preparations consisting essentially of a homogeneous endoglucanase component. The cellulase preparation may be employed in the treatment of cellulose-containing fabrics for harshness reduction, for color clarification, or to provide a localized variation in the color of such fabrics, or in the treatment of paper pulp.
摘要翻译：本发明涉及基本上由均匀的内切葡聚糖酶组分组成的纤维素酶制剂。纤维素酶制剂可用于处理含纤维素的织物以减少粗糙度，进行颜色澄清，或提供这种织物的颜色的局部变化，或纸浆的处理。

7. 发明授权

US5948672A Cellulase preparation comprising an endoglucanase enzyme 失效
标题翻译：包含内切葡聚糖酶的纤维素酶制剂
公开(公告)号：US5948672A
公开(公告)日：1999-09-07
申请号：US389423
申请日：1995-02-14
申请人： Grethe Rasmussen , Jan M.o slashed.ller Mikkelsen , Martin Schulein , Shamkant Anant Patkar , Fred Hagen , Carsten Mailand Hjort , Sven Hastrup
发明人： Grethe Rasmussen , Jan M.o slashed.ller Mikkelsen , Martin Schulein , Shamkant Anant Patkar , Fred Hagen , Carsten Mailand Hjort , Sven Hastrup
IPC分类号： C12N15/09 , C11D3/386 , C12N1/15 , C12N1/19 , C12N9/24 , C12N9/42 , C12N15/56 , C12N15/80 , C12Q1/68 , C12R1/645 , C12R1/69 , C12R1/77 , C12R1/865 , C12S3/04 , C12S3/08 , D06L4/40 , D06M16/00 , D06M101/00 , D06M101/02 , D06M101/06 , D06P5/00 , D06P5/08 , D06P5/13 , D06P5/15 , D21C5/02 , D21H11/20 , D21H17/00 , D21H21/10 , D21C1/00 , D21C3/00
CPC分类号： C12Q1/6895 , C11D3/38645 , C12N15/80 , C12N9/2437 , C12Y302/01004 , C12Y302/01039 , C12Y302/01091 , D06M16/003 , D06P5/137 , D06P5/158 , D21H17/005 , D21H21/10
摘要： A cellulase preparation consisting essentially of a homogeneous endoglucanase component which is immunoreactive with an antibody raised against a highly purified .about.43 kD endoglucanase derived from Humicola insolens, DSM 1800, or which is homogeneous to said .about.43 kD endoglucanase, may be employed in the treatment cellulose-containing fabrics for harshness reduction or color clarification or to provide a localized variation in the color of such fabrics, or it may be employed in the treatment of paper pulp.
摘要翻译：基本上由均匀的内切葡聚糖酶组分组成的纤维素酶制剂可以用于与针对来自Humicola insolens DSM 1800的高度纯化的差异43kD内切葡聚糖或与所述差异43kD内切葡聚糖酶相同的抗体产生免疫反应性的均一内切葡聚糖酶组分含有纤维素的织物用于粗糙度降低或颜色澄清或提供这种织物的颜色的局部变化，或者可以用于纸浆的处理。

8. 发明授权

US5686593A Enzyme capable of degrading cellulose or hemicellulose 失效
标题翻译：能够降解纤维素或半纤维素的酶
公开(公告)号：US5686593A
公开(公告)日：1997-11-11
申请号：US479939
申请日：1995-06-07
申请人： Helle Fabricius Woldike , Fred Hagen , Carsten Mailand Hjort , Sven Hastrup
发明人： Helle Fabricius Woldike , Fred Hagen , Carsten Mailand Hjort , Sven Hastrup
IPC分类号： C12N1/19 , C12N9/42 , C12N15/09 , C12R1/645 , C12R1/77 , C07H21/02 , A61K38/47 , C07H21/04 , G01N33/53
CPC分类号： C12N9/2437 , C12Y302/01004 , C12Y302/01091
摘要： The present invention relates to cellulose- or hemicellulose-degrading enzymes which is derivable from a fungus other than Trichoderma or Phanerochaete, and which comprises a carbohydrate binding domain homologous to a terminal A region of Trichoderma reesei cellulases, which carbohydrate binding domain comprises the following amino acid sequence. ##STR1## or a subsequence thereof capable of effecting binding of the enzyme to an insoluble cellulosic or hemicellulosic substrate.
摘要翻译：本发明涉及可从除木霉属或Phanerochaete之外的真菌衍生的纤维素或半纤维素降解酶，其包含与里氏木霉纤维素酶的末端A区同源的碳水化合物结合结构域，所述碳水化合物结合结构域包含以下氨基酸序列。将酶与不溶性纤维素或半纤维素基质结合起来。

9. 发明申请

US20100173390A1 Oxaloacetate Hydrolase Deficient Fungal Host Cells 有权
标题翻译：草酰乙酸水解酶缺乏真菌宿主细胞
公开(公告)号：US20100173390A1
公开(公告)日：2010-07-08
申请号：US12719648
申请日：2010-03-08
申请人： Carsten Mailand Hjort , Henrik Pedersen
发明人： Carsten Mailand Hjort , Henrik Pedersen
IPC分类号： C12N1/14
CPC分类号： C12Q1/6895 , C12N9/14
摘要： The present invention relates to isolated nucleic acid sequences encoding polypeptides having oxaloacetate hydrolase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as recombinant methods for producing the polypeptides.
摘要翻译：本发明涉及编码具有草酰乙酸水解酶活性的多肽的分离的核酸序列。本发明还涉及包含核酸序列的核酸构建体，载体和宿主细胞以及用于产生多肽的重组方法。

10. 发明授权

US5763254A Enzyme capable of degrading cellulose or hemicellulose 失效
标题翻译：能够降解纤维素或半纤维素的酶
公开(公告)号：US5763254A
公开(公告)日：1998-06-09
申请号：US483432
申请日：1995-06-07
申请人： Helle Fabricius Woldike , Fred Hagen , Carsten Mailand Hjort , Sven Hastrup
发明人： Helle Fabricius Woldike , Fred Hagen , Carsten Mailand Hjort , Sven Hastrup
IPC分类号： C12N1/19 , C12N9/42 , C12N15/09 , C12R1/645 , C12R1/77
CPC分类号： C12N9/2437 , C12Y302/01004 , C12Y302/01091
摘要： The present invention relates to isolated and purified cellulose- or hemicellulose-degrading enzymes, comprising a catalytically active domain, a carbohydrate binding domain and a linking B region which operably links the catalytically active domain and the carbohydrate binding domain, wherein the carbohydrate binding domain has the following core sequence: ##STR1## wherein the enzyme is endogenous to a strain of a fungus other than Trichoderma or Phanerochaete.
摘要翻译：本发明涉及分离和纯化的纤维素或半纤维素降解酶，其包含催化活性结构域，碳水化合物结合结构域和可操作地连接催化活性结构域和碳水化合物结合结构域的连接B区，其中碳水化合物结合结构域具有以下核心序列：其中所述酶对除木霉属或Phanerochaete之外的真菌菌株是内源的。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式