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    • 9. 发明授权
    • Transglutaminase from oomycetes
    • 来自卵菌的转谷氨酰胺酶
    • US06428993B1
    • 2002-08-06
    • US08881742
    • 1997-06-24
    • Lisbeth BechGrethe RasmussenTorben HalkierMariko OkadaLene Nonboe AndersenMarkus Sakari KauppinenThomas Sandal
    • Lisbeth BechGrethe RasmussenTorben HalkierMariko OkadaLene Nonboe AndersenMarkus Sakari KauppinenThomas Sandal
    • C12N910
    • C12Y203/02013A21D8/042C12N9/1044C14C11/00Y10S435/911
    • The present invention relates to transglutaminases and transglutaminase preparations obtained from lower fungi belonging to the class Oomycetes. Unprecedented high-level expression is achievable by growing these coenocytium forming organisms, especially the strains Pythium sp., Pythium irregulare, Pythium dissotocum, Pythium periilum (or P. periplocum), Pythium torulosum, Pythium ultimum, Pythium aphanidermatum, Phytophthora cactorum, Phytophthora palmivora, Phytophthora porri, Phytophthora infestans, Phytoph-thora megasperma, Phytophthora cinnamomi and Phytophthora cryptogea. Arecombinant transglutaminase has been cloned and expressed, the enzyme and enzyme preparations being useful for cross-linking proteins, e.g. in flour, baked products, meat products, fish products, cosmetics, cheese, milk products, gelled food products and leather finishing, or as a glutaminase, e.g. in bread and other baked glutein-containing food products.
    • 本发明涉及从属于卵菌纲的较低真菌获得的转谷氨酰胺酶和转谷氨酰胺酶制剂。 通过生长这些细胞形成的细菌,特别是菌株腐霉菌,不锈钢腐霉,腐霉腐霉(P. periplocum),腐皮腐,腐霉,腐霉,腐皮疫霉,致病疫霉(Phytophthora palmivora),可以实现前所未有的高水平表达 ,疫霉疫霉(Phytophthora porri),致病疫霉(Phytophthora infestans),致病疫霉(Phytoph-thora megasperma),肉桂疫霉(Phytophthora cinnamomi)和疫霉疫霉(Phytophthora cryptogea)。 克隆和表达重组转谷氨酰胺酶,酶和酶制剂可用于交联蛋白质,例如, 在面粉,烘焙食品,肉制品,鱼制品,化妆品,奶酪,乳制品,凝胶食品和皮革整理中,或作为谷氨酰胺酶。 在面包和其他烘烤含麸质的食品中。