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1. 发明授权

US5888775A Peptide synthesis and purification by fusion to penI protein or precipitation effective portion thereof 失效
标题翻译：通过与penI蛋白融合或其沉淀有效部分的肽合成和纯化
公开(公告)号：US5888775A
公开(公告)日：1999-03-30
申请号：US235178
申请日：1994-04-29
申请人： Rony Tal , Hing C. Wong , Clayton Casipit , Pierre-Andre Chavaillaz , Vaughan Wittman
发明人： Rony Tal , Hing C. Wong , Clayton Casipit , Pierre-Andre Chavaillaz , Vaughan Wittman
IPC分类号： G01N33/53 , A61K38/00 , A61K39/00 , A61P37/00 , A61P37/04 , C07K1/30 , C07K14/32 , C07K14/465 , C07K14/47 , C12N15/09 , C12N15/62 , C12P21/02 , C12R1/10 , C12R1/19 , G01N33/531 , C07K1/00 , C07K19/00
CPC分类号： C07K14/32 , C07K14/465 , C07K14/4706 , C07K14/4716 , C12N15/62 , C07K2319/50 , C07K2319/72 , C07K2319/80
摘要： The present invention provides novel methods for the synthesis and isolation and purification of a peptide of interest (target peptide). In particular, the invention relates to peptide synthesis, isolation and purification methods that comprise use of penI fusion polypeptides and related gene fusion constructs that encode such polypeptides.
摘要翻译：本发明提供用于合成和分离和纯化目标肽（靶肽）的新方法。特别地，本发明涉及肽合成，分离和纯化方法，其包括使用penI融合多肽和编码这种多肽的相关基因融合构建体。

2. 发明授权

US5744098A Device for the automatic examination of blood samples 失效
标题翻译：血液样本自动检查装置
公开(公告)号：US5744098A
公开(公告)日：1998-04-28
申请号：US460352
申请日：1995-06-02
申请人： Michael Kratzer , Volker Freiherr von der Goltz
发明人： Michael Kratzer , Volker Freiherr von der Goltz
IPC分类号： G01N1/00 , G01N33/49 , G01N35/00 , G01N35/02 , G01N33/48
CPC分类号： G01N35/02 , G01N33/4905 , G01N1/14 , G01N35/00009 , Y10T436/114165
摘要： A measuring unit for performing plural bleeding time analyses has a vessel divided into at least two chambers by a separating wall. Each chamber has a capillary coupled to an outlet in the chamber base wall, and the outlet is also in communication with an aperture of an apertured filter unit placed adjacent the base wall.
摘要翻译：用于执行多次出血时间分析的测量单元具有通过分隔壁分成至少两个室的容器。每个室具有耦合到室底壁中的出口的毛细管，并且出口也与邻近底壁放置的有孔过滤器单元的孔连通。

3. 发明授权

US5702912A Method to determine the concentration of anticoagulants 失效
标题翻译：确定抗凝剂浓度的方法
公开(公告)号：US5702912A
公开(公告)日：1997-12-30
申请号：US564986
申请日：1995-11-30
申请人： Hendrick Coenraad Hemker , Robert Johan Wagenvoord , Hans-Jurgen Kolde
发明人： Hendrick Coenraad Hemker , Robert Johan Wagenvoord , Hans-Jurgen Kolde
IPC分类号： C12Q1/56 , G01N33/53 , G01N33/86
CPC分类号： C12Q1/56 , G01N2333/815 , G01N2333/974 , G01N2400/40
摘要： The present invention relates to a method to determine the concentration of anticoagulants as a function of the inhibition of thrombin formation in an assay mixture. The assay mixture is composed of two reagents and the diluted plasma sample. The diluted plasma sample containing an anticoagulatory substance is mixed with a clotting factor reagent, which is a combination of an excess amount of purified coagulation factors of the endogenous system, phospholipids and a weak thrombin inhibitor, which can be potentiated by an anticoagulatory substance. Then a coagulation cascade activator and calcium chloride, an activator reagent, is added to the mixture and formed thrombin is measured after a chosen incubation time. A proportionality between the inhibition of the thrombin formation and the anticoagulatory active components allows for a determination of the concentration of anticoagulatory active components in the sample.
摘要翻译：本发明涉及一种确定抗凝血剂浓度作为测定混合物中凝血酶形成抑制的函数的方法。测定混合物由两种试剂和稀释的血浆样品组成。含有抗凝物质的稀释血浆样品与凝血因子试剂混合，凝血因子试剂是过量的内源性系统的纯化凝血因子，磷脂和弱凝血酶抑制剂的组合，其可以被抗凝物质加强。然后将凝血级联活化剂和氯化钙（活化剂）加入到混合物中，并在选择的孵育时间后测量形成的凝血酶。抑制凝血酶形成与抗凝活性成分之间的比例允许测定样品中抗凝活性成分的浓度。

4. 发明授权

US5651941A Sample tube carrier 失效
标题翻译：样品管载体
公开(公告)号：US5651941A
公开(公告)日：1997-07-29
申请号：US488894
申请日：1995-05-17
申请人： William A. Stark , Kempton H. Hardiman , Richard A. Scribner , John Mazza
发明人： William A. Stark , Kempton H. Hardiman , Richard A. Scribner , John Mazza
IPC分类号： B01L3/00 , B01L9/06 , B01L99/00 , G01N21/01 , G01N35/04 , B01L9/00
CPC分类号： B01L9/06 , G01N35/04 , B65G2201/0261
摘要： A self-adjusting linkable carrier for sample tubes, cuvettes, cells and the like comprising a longitudinally symmetrical vertically oriented polygonal body having two pairs of orthogonally disposed opposed vertical faces and a horizontal bottom sliding surface is disclosed. The top surface of the body is provided with a central bore opening into a vertical cavity adapted to releasably receive and retain a wide variety of sample tube configurations in a stable vertical orientation. Machine readable indicia may be provided on the external surface and vertical slots are provided in each external face to provide access to the central vertical cavity. A releasably locking horizontally aligned dovetail feature is provided to link adjacent carriers into conveniently handled racks or magazines which may be assembled and disassembled by machine. Additional horizontally sliding indexing elements are provided to facilitate the stable storage and transport of the carrier.
摘要翻译：公开了一种用于样品管，比色皿，电池等的自调节可连接载体，其包括纵向对称的垂直取向的多边体，其具有两对正交设置的相对的垂直面和水平底部滑动表面。本体的顶表面设置有一个中心孔，该中心孔开放到垂直腔中，适于可释放地接收和保持在稳定的垂直方向上的各种样品管构型。可以在外表面上提供机读标记，并且在每个外表面中设置垂直槽以提供对中心垂直腔的通路。提供可释放地锁定的水平对准的燕尾形特征，以将相邻的托架连接到可由机器组装和拆卸的便利处理的机架或杂志中。提供附加的水平滑动分度元件以便于载体的稳定的储存和运输。

5. 发明授权

US5625036A Preparation of prothrombin time reagents from recombinant human tissue factor and purified natural and synthetic phospholipids 失效
标题翻译：从重组人组织因子和纯化的天然和合成磷脂制备凝血酶原时间试剂
公开(公告)号：US5625036A
公开(公告)日：1997-04-29
申请号：US371052
申请日：1995-01-10
申请人： Pamela L. Hawkins , Liliana Tejidor , James Maynard , Kevin B. Johnson
发明人： Pamela L. Hawkins , Liliana Tejidor , James Maynard , Kevin B. Johnson
IPC分类号： C12Q1/56 , A61K9/127 , C07K14/745 , G01N33/86 , C07K4/00
CPC分类号： B82Y5/00 , A61K9/127 , A61K9/1277 , C07K14/745 , G01N33/86 , G01N2333/745 , G01N2405/04
摘要： A prothrombin time reagent is disclosed for use in a prothrombin time test. The reagent utilizes recombinant human tissue factor, phospholipids of a natural or synthetic origin, a buffer composition and calcium ion. Stabilizers and salts may also be utilized in the reagent. In addition, a method for creating lipid micelles containing tissue factor is also disclosed.
摘要翻译：公开了凝血酶原时间试剂用于凝血酶原时间试验。该试剂使用重组人组织因子，天然或合成来源的磷脂，缓冲组合物和钙离子。稳定剂和盐也可用于试剂中。此外，还公开了一种产生含有组织因子的脂质胶束的方法。

6. 发明授权

US5565328A Measurement of color reactions by monitoring a change of fluorescence 失效
标题翻译：通过监测荧光的变化来测量显色反应
公开(公告)号：US5565328A
公开(公告)日：1996-10-15
申请号：US579089
申请日：1995-12-27
申请人： Shoshana Bascomb , Frank J. Swenson , Ted Sand
发明人： Shoshana Bascomb , Frank J. Swenson , Ted Sand
IPC分类号： C12Q1/04 , C12Q1/26 , H01L21/306
CPC分类号： C12Q1/04 , Y10S435/968
摘要： The present invention relates to a method to determine the concentration of an unknown substance in a colorimetric reaction using a fluorometric reader. The change in color can be monitored by observing the change of fluorescence of a known amount of a suitable fluorophore in the reaction chamber. Under appropriate conditions the absorption spectrum of the chromophore overlaps with the emission spectrum of the fluorophore thereby allowing the change in fluorescence to be proportional to the intensity of color in the reaction and thus, proportional to the quantity of the substance of interest. Specifically, 8-methoxypyrene tri-sulfonic acid is used as the fluorophore.
摘要翻译：本发明涉及使用荧光读数器确定比色反应中未知物质的浓度的方法。可以通过观察已知量的合适的荧光团在反应室中的荧光变化来监测颜色变化。在合适的条件下，发色团的吸收光谱与荧光团的发射光谱重叠，从而允许荧光的变化与反应中颜色的强度成比例，因此与感兴趣的物质的数量成比例。具体地说，使用8-甲氧基芘三磺酸作为荧光团。

7. 发明授权

US5543327A Apparatus for conducting the cytotoxicity assays on tumor cells 失效
标题翻译：用于对肿瘤细胞进行细胞毒性测定的装置
公开(公告)号：US5543327A
公开(公告)日：1996-08-06
申请号：US279311
申请日：1994-07-22
申请人： Yu P. Yen-Maguire , Tom Cox , Fred Lewis
发明人： Yu P. Yen-Maguire , Tom Cox , Fred Lewis
IPC分类号： C12M1/20 , G01N33/50 , C12M1/34 , C12Q1/18 , C12Q1/20
CPC分类号： C12M23/12 , C12M25/14 , C12M41/46 , G01N33/5011 , G01N2800/52 , Y10S435/81
摘要： A method and apparatus for assaying the sensitivity of biopsied tumor cells to chemotherapeutic agents using a predetermined amount of chemotherapeutic agent in an easily deliverable form is disclosed and claimed.
摘要翻译：公开和要求保护的方法和装置，用于使用预定量的化学治疗剂以易于递送的形式测定活检肿瘤细胞对化学治疗剂的敏感性。

8. 发明授权

US5532941A Inter-laboratory performance monitoring system 失效
标题翻译：实验室间性能监测系统
公开(公告)号：US5532941A
公开(公告)日：1996-07-02
申请号：US272239
申请日：1994-07-08
申请人： Lawrence I. Lin
发明人： Lawrence I. Lin
IPC分类号： G01D21/00 , G01N35/00 , G05B19/418 , G08C25/00 , G06F15/16 , G06G7/48
CPC分类号： G01N35/00594 , G01N35/00871 , G05B19/41875 , G08C25/00 , G01N2035/00653 , G01N35/00712 , G05B2219/31392 , G05B2219/32187 , Y02P90/22
摘要： A system and method for producing quality control evaluation information for each instrument in a large group of instruments making up a peer group which periodically (such as daily) run a set of control samples from a common lot of control materials. The control data from all instruments is reported electronically to a central station which collects all of the data and stores it along with identification information. The central station selects a golden peer group of the instruments which meet certain minimum operating criteria, and determines control targets from the golden peer group using techniques which assure horizontal and vertical robustness, so as to eliminate the need for any manual editing of the control data. A concordance correlation coefficient is determined for each instrument against the control targets, with the CCC covering an interval (such as a month). The distribution of CCC's for all instruments in the peer group is determined, and each instrument is rated with respect to the distribution. The CCC evaluation is also used to select the golden peer group for the next interval.
摘要翻译：在构成对等组织的大型仪器组中每个仪器生产质量控制评估信息的系统和方法，其定期（例如每天）从一大批控制材料运行一组对照样品。来自所有仪器的控制数据以电子方式报告给收集所有数据并将其与识别信息一起存储的中心站。中央电视台选择符合某些最低运作标准的黄金同伴组，并通过确保水平和垂直稳健性的技术确定黄金同伴组的控制目标，从而消除对控制数据的任何手动编辑的需要。每个仪器针对控制目标确定一致性相关系数，CCC覆盖间隔（如一个月）。确定了CCC对同行群体中所有工具的分配情况，并对每个工具进行分配评估。 CCC评估也用于在下一个时间间隔内选择黄金对等体组。

9. 发明授权

US5516637A Method involving display of protein binding pairs on the surface of bacterial pili and bacteriophage 失效
标题翻译：涉及在细菌菌毛和噬菌体表面显示蛋白质结合对的方法
公开(公告)号：US5516637A
公开(公告)日：1996-05-14
申请号：US258026
申请日：1994-06-10
申请人： Grace P. Huang , Peter R. Rhode , Jeffrey R. Stinson , Hing C. Wong
发明人： Grace P. Huang , Peter R. Rhode , Jeffrey R. Stinson , Hing C. Wong
IPC分类号： G01N33/53 , C07K14/245 , C07K16/00 , C07K19/00 , C12N1/21 , C12N15/09 , C12N15/10 , C12N15/70 , C12P21/04 , C12Q1/02 , C12Q1/70 , C12R1/19 , C40B40/02 , G01N33/569 , C12Q1/68 , G01N33/554
CPC分类号： C40B40/02 , C07K14/245 , C07K16/00 , C12N15/1037 , C12N15/70 , C12Q1/02 , C12Q1/70 , C07K2319/00 , C07K2319/02
摘要： The present invention relates to a fusion protein, comprising a pilin protein or a portion thereof and a heterologous polypeptide (target protein). In a preferred embodiment it relates to a method for displaying the target protein on the outer surface of a bacterial host cell capable of forming pilus. In certain embodiments, it is desirable that the pilus is a receptor for bacteriophage attachment and infection. The F pilus is preferred.
摘要翻译：本发明涉及一种融合蛋白，其包含比林蛋白或其部分和异源多肽（靶蛋白）。在一个优选实施方案中，其涉及在能够形成菌毛的细菌宿主细胞的外表面上显示靶蛋白的方法。在某些实施方案中，希望菌毛是噬菌体附着和感染的受体。 F菌毛是优选的。

10. 发明授权

US5506105A In situ assay of amplified intracellular mRNA targets 失效
标题翻译：扩增细胞内mRNA靶的原位测定
公开(公告)号：US5506105A
公开(公告)日：1996-04-09
申请号：US216233
申请日：1994-03-22
申请人： Paul V. Haydock
发明人： Paul V. Haydock
IPC分类号： C12Q1/68 , C12Q1/70 , C12P19/34
CPC分类号： C12Q1/708 , C12Q1/6841 , C12Q1/6865
摘要： In an in situ hybridization assay, cells are fixed under conditions which preserve essential morphology, followed by transcription-based nucleic acid amplification of intracellular mRNA targets. The amplified targets are visualized by hybridizing labelled probes to the target sequences. The assay thereby provides a convenient method for detecting specific gene expression associated with morphologic characteristics of diagnostic significance.
摘要翻译：在原位杂交测定中，细胞在保持基本形态，然后是基于转录的核酸扩增细胞内mRNA靶标的条件下固定。通过将标记的探针杂交到靶序列来显现扩增的目标。因此，该测定提供了用于检测与具有诊断意义的形态学特征相关的特异性基因表达的方便方法。

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