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71. 发明申请

US20090000948A1 Methods for Improving Efficiency of Cell Electroporation Using Dielectrophoreses 审中-公开
标题翻译：提高细胞电穿孔效率的方法
公开(公告)号：US20090000948A1
公开(公告)日：2009-01-01
申请号：US12097409
申请日：2007-01-04
申请人： Lei Wang , Lei Wang , Min Guo , Jing Cheng
发明人： Lei Wang , Lei Wang , Min Guo , Jing Cheng
IPC分类号： G01N27/26 , G01N27/00
CPC分类号： C12N13/00 , C12M23/16 , C12M33/00 , C12M35/02
摘要： The present invention provides methods for enhancing the efficiency of cell electroporation using dielectrophoresis-assisted cell localization and uses thereof in a microfluidic biochip system. Cells are first subject to dielectrophoresis and localized to regions where the electric field intensity is high enough to render cells electroporated. The invention enhances the efficiency of in situ cell electroporation on a traditional microfluidic biochip.
摘要翻译：本发明提供使用介电电泳辅助细胞定位提高细胞电穿孔效率的方法及其在微流体生物芯片系统中的用途。细胞首先进行介导电泳，并定位于电场强度足以使细胞电穿孔的区域。本发明增强了传统微流体生物芯片上原位细胞电穿孔的效率。

72. 发明申请

US20080220979A1 Rapid Method To Detect Nucleic Acid Molecules 审中-公开
标题翻译：检测核酸分子的快速方法
公开(公告)号：US20080220979A1
公开(公告)日：2008-09-11
申请号：US10568206
申请日：2003-08-27
申请人： Dong Wang , Gang Li , Xuemei Ma , Chengxun Liu , Yuxiang Zhou , Jing Cheng
发明人： Dong Wang , Gang Li , Xuemei Ma , Chengxun Liu , Yuxiang Zhou , Jing Cheng
IPC分类号： C40B30/04 , C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6837 , C12Q2565/501 , C12Q2565/513 , C12Q2565/507
摘要： This invention relates to the field of detecting nucleic acid molecules using microarrays. The invention provides a method for detecting a target nucleic acid molecule in a biological sample by hybridizing a cell lysate directly probes immobilized on microarrays without any nucleic acid purification.
摘要翻译：本发明涉及使用微阵列检测核酸分子的领域。本发明提供了通过将固定在微阵列上的细胞裂解物直接杂交而不进行任何核酸纯化来检测生物样品中的靶核酸分子的方法。

73. 发明申请

US20080047832A1 Integrated portable biological detection system 失效
标题翻译：综合便携式生物检测系统
公开(公告)号：US20080047832A1
公开(公告)日：2008-02-28
申请号：US11702417
申请日：2007-02-05
申请人： Jing Cheng , Lei Wu , Michael Heller , Edward Sheldon , Jonathan Diver , James O'Connell , Dan Smolko , Shila Jalali , David Willoughby
发明人： Jing Cheng , Lei Wu , Michael Heller , Edward Sheldon , Jonathan Diver , James O'Connell , Dan Smolko , Shila Jalali , David Willoughby
IPC分类号： C12M1/42
CPC分类号： C12Q1/24 , B01L3/502 , B01L2300/0636 , B01L2300/0645 , B01L2300/0877 , B01L2400/0424 , B01L2400/0487 , G01N21/6428
摘要： We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.
摘要翻译：我们通过介电电泳在微细电子芯片中进行细胞和癌细胞与外周血液分离。通过用荧光染料染色细胞核，然后激光诱导荧光成像来检查分离的细胞。我们还通过电子方式从分离的细胞中释放DNA和RNA，并通过DNA扩增检测特异性标记序列，然后与固定的捕获探针进行电子杂交。提出了建立“芯片实验室”系统的努力，其中包括选择DNA探针，染料，试剂和完全集成的便携式仪器的原型。

74. 发明申请

US20070184433A1 Microparticle based biochip systems and uses thereof 审中-公开
标题翻译：基于微粒的生物芯片系统及其用途
公开(公告)号：US20070184433A1
公开(公告)日：2007-08-09
申请号：US10557328
申请日：2003-07-14
申请人： Shengce Tao , Guoqing Wang , Jing Cheng
发明人： Shengce Tao , Guoqing Wang , Jing Cheng
IPC分类号： C12Q1/70 , C12Q1/68 , C12M3/00 , C07H21/04
CPC分类号： C12Q1/706 , B01L3/508 , B01L2200/0647 , B01L2300/0636 , B01L2300/0819 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , C12Q1/6834 , C12Q1/689 , G01N33/552 , G01N33/558 , C12Q2563/149 , C12Q2563/143
摘要： This invention relates generally to the field of analyte assays. In particular, the invention provides a device for analyzing an analyte, which device comprises, inter alia, various means for moving analytes and other items to facilitate binding between analytes and their binding reagents immobilized on a surface and to facilitate clearance of undesirable items away from analyte-binding reagent interaction area to reduce background noise in the assay. Methods for analyzing an analyte using the devices are also disclosed.
摘要翻译：本发明一般涉及分析物测定领域。特别地，本发明提供了一种用于分析分析物的装置，该装置尤其包括用于移动分析物和其它物品的各种装置，以促进分析物与其固定在表面上的结合试剂之间的结合，并有利于将不期望的物品远离分析物结合试剂相互作用区域，以减少测定中的背景噪音。还公开了使用这些装置分析分析物的方法。

75. 发明申请

US20070059700A1 Methods and compositions for optimizing multiplex pcr primers 审中-公开
标题翻译：优化多重pcr引物的方法和组合物
公开(公告)号：US20070059700A1
公开(公告)日：2007-03-15
申请号：US10559951
申请日：2003-05-09
申请人： Shengce Tao , Jing Cheng
发明人： Shengce Tao , Jing Cheng
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q2537/143 , C12Q2525/155
摘要： This invention relates generally to the field of PCR. In particular, the invention provides methods, compositions and kits for optimizing multiplex PCR primers using, inter alia, a plurality of 5′ and 3′ specific primers and a 5′ and a 3′ universal primer at various ratios.
摘要翻译：本发明一般涉及PCR领域。特别地，本发明提供了使用尤其是多种5'和3'特异性引物以及各种比例的5'和3'通用引物优化多重PCR引物的方法，组合物和试剂盒。

76. 发明申请

US20070042350A1 Methods and compositions for detecting sars virus and other infectious agents 审中-公开
标题翻译：用于检测莎草病毒和其他感染因子的方法和组合物
公开(公告)号：US20070042350A1
公开(公告)日：2007-02-22
申请号：US10564378
申请日：2003-07-14
申请人： Ze Li , Shengee Tao , Jing Cheng
发明人： Ze Li , Shengee Tao , Jing Cheng
IPC分类号： C12Q1/70 , C12Q1/68 , C12M3/00
CPC分类号： C12Q1/701 , C12Q1/6837 , C12Q1/686 , Y02A50/54
摘要： This invention relates generally to the field of virus detection. In particular, the invention provides chips, probes, primers, kits and methods for amplifying and detecting SARS-CoV nucleotides sequence. The clinical and other uses of the present chips, probes, primers, kits and methods are also contemplated.
摘要翻译：本发明一般涉及病毒检测领域。特别地，本发明提供用于扩增和检测SARS-CoV核苷酸序列的芯片，探针，引物，试剂盒和方法。还考虑了本发明芯片，探针，引物，试剂盒和方法的临床和其它用途。

77. 发明申请

US20070037140A1 Methods and compositions for detecting sars virus 审中-公开
标题翻译：检测萨尔病毒的方法和组成
公开(公告)号：US20070037140A1
公开(公告)日：2007-02-15
申请号：US10556182
申请日：2003-05-09
申请人： Ze Li , Yiming Zhou , Shengce Tao , Jing Cheng , Jianwei Wang , Tao Hong , Yun Cheng , Quanzhong Shan
发明人： Ze Li , Yiming Zhou , Shengce Tao , Jing Cheng , Jianwei Wang , Tao Hong , Yun Cheng , Quanzhong Shan
IPC分类号： C12Q1/70 , C12Q1/68 , C12M1/34
CPC分类号： C07H21/00 , C12Q1/701
摘要： This invention relates generally to the field of virus detection. In particular, the invention provides chips, probes, primers, kits and methods for amplifying and detecting SARS-CoV nucleotide sequence. The clinical and other uses of the present chips, probes, primers, kits and methods are also contemplated.
摘要翻译：本发明一般涉及病毒检测领域。特别地，本发明提供用于扩增和检测SARS-CoV核苷酸序列的芯片，探针，引物，试剂盒和方法。还考虑了本发明芯片，探针，引物，试剂盒和方法的临床和其它用途。

78. 发明申请

US20060233648A1 Method for fluid transfer and the micro peristaltic pump 有权
标题翻译：流体转移方法和微蠕动泵
公开(公告)号：US20060233648A1
公开(公告)日：2006-10-19
申请号：US10543619
申请日：2003-07-14
申请人： Chengxun Liu , Min Guo , Jing Cheng
发明人： Chengxun Liu , Min Guo , Jing Cheng
IPC分类号： F04B49/06
CPC分类号： F04B43/02 , F04B43/028 , F04B43/14
摘要： This invention relates generally to a method for fluid transfer and a micro peristaltic pump based upon the method. The micro peristaltic pump, which comprises: a) an actuating part compring a motor and a first force effector driven by the motor; b) a cartridge part comprising an elastic membrane attached to a cartridge body, wherein said elastic membrane attached to said cartridge body forms an enclosed space within the cartridge body comprising at least three chambers, and the cartridge also comprising a second force effector which interacts with the first force effector; c) at least three said chambers have inlets and outlets, which are sealingly connected in tandem. Compared with prior art in the micro pump field, the micro peristaltic pump in this invention can generate much greater actuating forces with equivalent overall size. In addition, since the micro peristaltic pump is composed by two separate parts, with one entirely close and disposable and the other reusable, the performance of this micro pump is very high with low cost for production.
摘要翻译：本发明一般涉及一种基于该方法的流体转移方法和微型蠕动泵。微型蠕动泵，包括：a）一个驱动部件，包括一个马达和一个由马达驱动的第一个力效应器; b）包括附接到盒体的弹性膜的盒部件，其中附接到所述盒体的所述弹性膜在所述盒体内形成包括至少三个室的封闭空间，并且所述盒还包括与第二力效应器相互作用的第二力效应器第一个力效应器; c）至少三个所述腔室具有串联密封连接的入口和出口。与微泵领域的现有技术相比，本发明的微蠕动泵可以产生相当大的致动力。另外，由于微型蠕动泵由两个独立的部件组成，其中一个完全紧密且一次性可重复使用，另一个可重复使用，该微型泵的性能非常高，生产成本低。

79. 发明授权

US06989086B2 Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis 失效
标题翻译：通过介电电泳在生物电子芯片上无生物颗粒的分离
公开(公告)号：US06989086B2
公开(公告)日：2006-01-24
申请号：US09905755
申请日：2001-07-13
申请人： Jing Cheng , Edward L. Sheldon, III , Lei Wu , James P. O'Connell
发明人： Jing Cheng , Edward L. Sheldon, III , Lei Wu , James P. O'Connell
IPC分类号： G01N27/26 , G01N27/447
CPC分类号： B01L3/5027 , B01D57/02 , B01J19/0093 , B01J2219/00853 , B01J2219/00914 , B01L3/502753 , B01L3/502761 , B01L2200/0631 , B01L2200/0647 , B01L2200/0668 , B01L2200/10 , B01L2300/0636 , B01L2300/0645 , B01L2300/0816 , B01L2300/0877 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0496 , B01L2400/0644 , B03C5/026 , B03C5/028 , C12N13/00 , G01N27/44743 , H01L29/6656
摘要： The present invention comprises devices and methods for performing channel-less separation of cell particles by dielectrophoresis, DC high voltage-pulsed electronic lysis of separated cells, separation of desired components from crude mixtures such as cell lysates, and/or enzymatic reaction of such lysates, all of which can be conducted on a single bioelectronic chip. A preferred embodiment of the present invention comprises a cartridge (10) including a microfabricated silicon chip (12) on a printed circuit board (14) and a flow cell (16) mounted to the chip (12) to form a flow chamber. The cartridge (10) also includes output pins (22) for electronically connecting the cartridge (10) to an electronic controller. The chip (12) % includes a plurality of circular microelectrodes (24) which are preferably coated with a protective permeation layer. Specific cells from various cell mixtures were separated, lysed, and enzymatically digested on the chip.
摘要翻译：本发明包括用于通过介电电泳，DC分离细胞的DC高压脉冲电离裂解，细胞裂解物等粗制混合物分离所需组分和/或这些裂解物的酶反应来进行细胞颗粒的无通道分离的装置和方法所有这些都可以在单个生物电子芯片上进行。本发明的优选实施例包括在印刷电路板（14）上包括微细硅芯片（12）的盒（10）和安装到芯片（12）上以形成流动室的流动池（16）。盒（10）还包括用于将盒（10）电连接到电子控制器的输出销（22）。芯片（12）％包括多个圆形微电极（24），其优选地涂覆有保护性渗透层。分离来自各种细胞混合物的特异性细胞，裂解，并在芯片上酶消化。

80. 发明授权

US06403367B1 Integrated portable biological detection system 失效
标题翻译：综合便携式生物检测系统
公开(公告)号：US06403367B1
公开(公告)日：2002-06-11
申请号：US09470448
申请日：1999-12-22
申请人： Jing Cheng , Lei Wu , Michael J. Heller , Ed Sheldon , Jonathan Diver , James P. O'Connell , Dan Smolko , Shila Jalali , David Willoughby
发明人： Jing Cheng , Lei Wu , Michael J. Heller , Ed Sheldon , Jonathan Diver , James P. O'Connell , Dan Smolko , Shila Jalali , David Willoughby
IPC分类号： C12M134
CPC分类号： G11C13/0019 , B01D57/02 , B01J19/0046 , B01J19/0093 , B01J2219/00317 , B01J2219/00585 , B01J2219/0059 , B01J2219/00653 , B01J2219/00659 , B01J2219/00686 , B01J2219/00689 , B01J2219/00713 , B01J2219/00722 , B01J2219/00853 , B01J2219/00914 , B01L3/5027 , B01L3/502753 , B01L3/502761 , B01L7/52 , B01L2200/0631 , B01L2200/0647 , B01L2200/0668 , B01L2200/10 , B01L2300/0636 , B01L2300/0645 , B01L2300/0816 , B01L2300/087 , B01L2300/0877 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0496 , B01L2400/0644 , B03C5/026 , B03C5/028 , B82Y5/00 , B82Y10/00 , C07H21/00 , C07K1/045 , C12N13/00 , C12Q1/6806 , C12Q1/6825 , C12Q1/6832 , C12Q1/6837 , C40B40/06 , C40B60/14 , G01N27/44743 , G11C13/0014 , G11C13/04 , G11C19/00 , H01L25/50 , H01L2924/0002 , C12Q2565/515 , C12Q2565/607 , C12Q2565/629 , C12Q2525/107 , C12Q2531/119 , C12Q2531/101 , C12Q2531/113 , H01L2924/00
摘要： We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.
摘要翻译：我们通过介电电泳在微细电子芯片中进行细胞和癌细胞与外周血液分离。通过用荧光染料染色细胞核，然后激光诱导荧光成像来检查分离的细胞。我们还通过电子方式从分离的细胞中释放DNA和RNA，并通过DNA扩增检测特异性标记序列，然后与固定的捕获探针进行电子杂交。提出了建立“芯片实验室”系统的努力，其中包括选择DNA探针，染料，试剂和完全集成的便携式仪器的原型。

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