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1. 发明申请

US20080047832A1 Integrated portable biological detection system 失效
标题翻译：综合便携式生物检测系统
公开(公告)号：US20080047832A1
公开(公告)日：2008-02-28
申请号：US11702417
申请日：2007-02-05
申请人： Jing Cheng , Lei Wu , Michael Heller , Edward Sheldon , Jonathan Diver , James O'Connell , Dan Smolko , Shila Jalali , David Willoughby
发明人： Jing Cheng , Lei Wu , Michael Heller , Edward Sheldon , Jonathan Diver , James O'Connell , Dan Smolko , Shila Jalali , David Willoughby
IPC分类号： C12M1/42
CPC分类号： C12Q1/24 , B01L3/502 , B01L2300/0636 , B01L2300/0645 , B01L2300/0877 , B01L2400/0424 , B01L2400/0487 , G01N21/6428
摘要： We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.
摘要翻译：我们通过介电电泳在微细电子芯片中进行细胞和癌细胞与外周血液分离。通过用荧光染料染色细胞核，然后激光诱导荧光成像来检查分离的细胞。我们还通过电子方式从分离的细胞中释放DNA和RNA，并通过DNA扩增检测特异性标记序列，然后与固定的捕获探针进行电子杂交。提出了建立“芯片实验室”系统的努力，其中包括选择DNA探针，染料，试剂和完全集成的便携式仪器的原型。

2. 发明授权

US07172896B2 Integrated portable biological detection system 失效
标题翻译：综合便携式生物检测系统
公开(公告)号：US07172896B2
公开(公告)日：2007-02-06
申请号：US10163835
申请日：2002-06-05
申请人： Jing Cheng , Lei Wu , Michael J. Heller , Ed Sheldon , Jonathan Diver , James P. O'Connell , Dan Smolko , Shila Jalali , David Willoughby
发明人： Jing Cheng , Lei Wu , Michael J. Heller , Ed Sheldon , Jonathan Diver , James P. O'Connell , Dan Smolko , Shila Jalali , David Willoughby
IPC分类号： C12M3/00
CPC分类号： G11C13/0019 , B01D57/02 , B01J19/0046 , B01J19/0093 , B01J2219/00317 , B01J2219/00585 , B01J2219/0059 , B01J2219/00653 , B01J2219/00659 , B01J2219/00686 , B01J2219/00689 , B01J2219/00713 , B01J2219/00722 , B01J2219/00853 , B01J2219/00914 , B01L3/5027 , B01L3/502753 , B01L3/502761 , B01L7/52 , B01L2200/0631 , B01L2200/0647 , B01L2200/0668 , B01L2200/10 , B01L2300/0636 , B01L2300/0645 , B01L2300/0816 , B01L2300/087 , B01L2300/0877 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0496 , B01L2400/0644 , B03C5/026 , B03C5/028 , B82Y5/00 , B82Y10/00 , C07H21/00 , C07K1/045 , C12N13/00 , C12Q1/6806 , C12Q1/6825 , C12Q1/6832 , C12Q1/6837 , C40B40/06 , C40B60/14 , G01N27/44743 , G11C13/0014 , G11C13/04 , G11C19/00 , H01L25/50 , H01L2924/0002 , C12Q2565/515 , C12Q2565/607 , C12Q2565/629 , C12Q2525/107 , C12Q2531/119 , C12Q2531/101 , C12Q2531/113 , H01L2924/00
摘要： We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.
摘要翻译：我们通过介电电泳在微细电子芯片中进行细胞和癌细胞与外周血液分离。通过用荧光染料染色细胞核，然后激光诱导荧光成像来检查分离的细胞。我们还通过电子方式从分离的细胞中释放DNA和RNA，并通过DNA扩增检测特异性标记序列，然后与固定的捕获探针进行电子杂交。提出了建立“芯片实验室”系统的努力，其中包括选择DNA探针，染料，试剂和完全集成的便携式仪器的原型。

3. 发明授权

US06403367B1 Integrated portable biological detection system 失效
标题翻译：综合便携式生物检测系统
公开(公告)号：US06403367B1
公开(公告)日：2002-06-11
申请号：US09470448
申请日：1999-12-22
申请人： Jing Cheng , Lei Wu , Michael J. Heller , Ed Sheldon , Jonathan Diver , James P. O'Connell , Dan Smolko , Shila Jalali , David Willoughby
发明人： Jing Cheng , Lei Wu , Michael J. Heller , Ed Sheldon , Jonathan Diver , James P. O'Connell , Dan Smolko , Shila Jalali , David Willoughby
IPC分类号： C12M134
CPC分类号： G11C13/0019 , B01D57/02 , B01J19/0046 , B01J19/0093 , B01J2219/00317 , B01J2219/00585 , B01J2219/0059 , B01J2219/00653 , B01J2219/00659 , B01J2219/00686 , B01J2219/00689 , B01J2219/00713 , B01J2219/00722 , B01J2219/00853 , B01J2219/00914 , B01L3/5027 , B01L3/502753 , B01L3/502761 , B01L7/52 , B01L2200/0631 , B01L2200/0647 , B01L2200/0668 , B01L2200/10 , B01L2300/0636 , B01L2300/0645 , B01L2300/0816 , B01L2300/087 , B01L2300/0877 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0496 , B01L2400/0644 , B03C5/026 , B03C5/028 , B82Y5/00 , B82Y10/00 , C07H21/00 , C07K1/045 , C12N13/00 , C12Q1/6806 , C12Q1/6825 , C12Q1/6832 , C12Q1/6837 , C40B40/06 , C40B60/14 , G01N27/44743 , G11C13/0014 , G11C13/04 , G11C19/00 , H01L25/50 , H01L2924/0002 , C12Q2565/515 , C12Q2565/607 , C12Q2565/629 , C12Q2525/107 , C12Q2531/119 , C12Q2531/101 , C12Q2531/113 , H01L2924/00
摘要： We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.
摘要翻译：我们通过介电电泳在微细电子芯片中进行细胞和癌细胞与外周血液分离。通过用荧光染料染色细胞核，然后激光诱导荧光成像来检查分离的细胞。我们还通过电子方式从分离的细胞中释放DNA和RNA，并通过DNA扩增检测特异性标记序列，然后与固定的捕获探针进行电子杂交。提出了建立“芯片实验室”系统的努力，其中包括选择DNA探针，染料，试剂和完全集成的便携式仪器的原型。

4. 发明申请

US20110039258A1 METHODS AND COMPOSITIONS FOR DIFFERENTIAL EXPANSION OF FETAL CELLS IN MATERNAL BLOOD AND THEIR USE 审中-公开
标题翻译：方法和组合物在宫颈细胞中的差异扩大及其使用
公开(公告)号：US20110039258A1
公开(公告)日：2011-02-17
申请号：US12445732
申请日：2007-10-15
申请人： Patricia McNeeley , Philippe Marchand , Jonathan Diver
发明人： Patricia McNeeley , Philippe Marchand , Jonathan Diver
IPC分类号： C12Q1/68 , C12N5/02 , C12Q1/02
CPC分类号： C12N5/0634 , C12N2500/90 , C12N2500/98 , C12N2501/125 , C12N2501/23
摘要： Disclosed is a method and compositions for the differential expansion of fetal cells over maternal cells. In the method, cells from a sample of maternal blood containing CD34+ cells of both maternal and fetal origin are incubated in the presence of Stem Cell Factor in serum free media. It has been discovered that incubation of fetal cells in the presence of SCF will preferentially expand the fetal cells relative to adult cells. Fetal cells can also be identified, enriched or obtained by differential expansion of the fetal cells during colony formation. It has been discovered that differential expansion of fetal cells can result in colonies of fetal cells that are larger than colonies of adult cells. The fetal CD34+ cells can be expanded without generation of significant clonal genetic artifacts during expansion. Also disclosed is a method and compositions for producing differentiated fetal cells. It has been discovered that differentiated fetal cells have markers that distinguish the fetal cells from adult cells. Also disclosed are fetal cells made or obtained using the disclosed methods. For example, disclosed are expanded and/or differentiated fetal cells. The disclosed fetal cells can be used for any purpose and in any way that fetal cells can be used. The disclosed fetal cells are particularly useful for prenatal analysis of a gestating fetus.
摘要翻译：公开了胎儿细胞在母细胞上差异扩增的方法和组合物。在该方法中，含有母体和胎儿来源的含有CD34 +细胞的母体血液样品的细胞在无血清培养基中的干细胞因子存在下孵育。已经发现，在SCF存在下胎儿细胞的孵育将相对于成体细胞优先扩张胎儿细胞。胎儿细胞也可以在菌落形成期间通过胎儿细胞的差异扩增来鉴定，富集或获得。已经发现，胎儿细胞的差异扩增可导致比成体细胞的集落大的胎儿细胞的集落。胎儿CD34 +细胞可以在扩增期间扩增而不产生显着的克隆遗传伪像。还公开了用于产生分化的胎儿细胞的方法和组合物。已经发现，分化的胎儿细胞具有区分胎儿细胞和成体细胞的标记。还公开了使用所公开的方法制造或获得的胎儿细胞。例如，所公开的是扩增和/或分化的胎儿细胞。所公开的胎儿细胞可以用于任何目的，并且可以以任何方式使用胎儿细胞。所公开的胎儿细胞特别可用于孕胎分娩。

5. 发明申请

US20050136441A1 Primer extension detection methods on active electronic microarrays 审中-公开
标题翻译：活性电子微阵列引物扩增检测方法
公开(公告)号：US20050136441A1
公开(公告)日：2005-06-23
申请号：US10942565
申请日：2004-09-15
申请人： John Carrino , Louis Gerrue , Jonathan Diver
发明人： John Carrino , Louis Gerrue , Jonathan Diver
IPC分类号： C12Q1/68 , C12Q1/6825 , C12Q1/6837 , C12Q1/6844 , C40B40/06 , C40B60/14 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6844 , B01J2219/00317 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00614 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00644 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , C12Q1/6825 , C12Q1/6837 , C40B40/06 , C40B60/14 , Y10T436/143333 , C12Q2531/113 , C12Q2535/125 , C12Q2565/501
摘要： This invention relates to methods for detecting the extent of hybridization of a nucleic acid in a sample to a probe nucleic acid sequence by electronically hybridizing the nucleic acid in the sample to the probe, utilizing the hybridized nucleic acid as a template in a nucleic acid polymerase reaction to extend the bound probe and incorporate a labeled nucleotide, and detecting the labeled product. This invention also relates to methods of detecting the extent of hybridization of a plurality of nucleic acids in a sample to a plurality of nucleic acid probes using similar methods.
摘要翻译：本发明涉及通过使用杂交核酸作为模板在核酸聚合酶中将样品中的核酸与探针电子杂交来检测样品中的核酸与探针核酸序列的杂交程度的方法扩增结合探针并掺入标记核苷酸并检测标记产物的反应。本发明还涉及使用类似方法检测样品中多种核酸与多种核酸探针的杂交程度的方法。

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