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    • 70. 发明授权
    • Discrete plasmid construction from chromosomal genes in pseudomonas
    • 假单胞菌染色体基因的离散质粒构建
    • US3923603A
    • 1975-12-02
    • US49369174
    • 1974-08-01
    • GEN ELECTRIC
    • CHAKRABARTY ANANDA MFRIELLO DENISE A
    • C12N15/52C12N15/78C12B1/00
    • C12N15/52C12N15/78Y10S435/82Y10S435/874Y10S435/877
    • A transfer plasmid (factor K) is transferred from a first Pseudomonas organism to a second Pseudomonas organism. Factor K induces the mobilization of genes from the chromosome of the second Pseudomonas organism and under proper conditions will transfer the mobilized genes to a recipient organism in which those particular genes have been deleted. The factor K and the mobilized genes enter the recipient and become established there as stable separate plasmids constituting a plasmid aggregrate. Non-transmissible artificial hydrocarbon degradative plasmids have been created in Pseudomonas organisms in this manner and (with factor K present in the host to accomplish the transfer) have been transmitted to other Pseudomonas recipients.
    • 转移质粒(因子K)从第一假单胞菌生物体转移到第二假单胞菌生物体。 因子K诱导来自第二种假单胞菌生物体的染色体的基因的移动,并且在适当的条件下将移动的基因转移到这些特定基因已被缺失的受体生物体中。 因子K和移动的基因进入受体并在其中建立为稳定的构成质粒聚集体的分离质粒。 已经以这种方式在假单胞菌生物体中产生了不可传播的人造烃降解质粒,并且(将宿主中存在的K因子完成转移)已被传递给其他假单胞菌受体。