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61. 发明授权

US07480042B1 Luminescence reference standards 有权
标题翻译：发光参考标准
公开(公告)号：US07480042B1
公开(公告)日：2009-01-20
申请号：US11173609
申请日：2005-06-30
申请人： J. Michael Phillips , Aldrich N. K. Lau , Mark F. Oldham , Kevin S. Bodner , Steven J. Boege , Donald R. Sandell , David H. Tracy
发明人： J. Michael Phillips , Aldrich N. K. Lau , Mark F. Oldham , Kevin S. Bodner , Steven J. Boege , Donald R. Sandell , David H. Tracy
IPC分类号： G01J1/10
CPC分类号： G01N21/278 , B82Y15/00 , G01J1/10 , G01N21/01 , G01N21/4785 , G01N21/6452 , G01N21/76 , G01N2201/06113 , G01N2201/0612 , G01N2201/062 , G01N2201/0691 , G01N2201/0697 , Y10S977/943 , Y10S977/949
摘要： Systems, and components thereof, for detecting and/or analyzing light. These systems can include, among others, optical reference standards for calibrating, validating, and/or monitoring light-detection systems, before, during, and/or after sample analysis.
摘要翻译：系统及其组件，用于检测和/或分析光。样品分析之前，期间和/或之后，这些系统可以包括用于校准，验证和/或监测光检测系统的光学参考标准。

62. 发明授权

US07459068B2 Multi-capillary electrophoresis apparatus 有权
标题翻译：多毛细管电泳仪
公开(公告)号：US07459068B2
公开(公告)日：2008-12-02
申请号：US10309729
申请日：2002-12-04
申请人： Eric S. Nordman , Miho Ozawa , Masaya Kojima , Ryoji Inaba , Yoshitaka Kodama , Motohiro Yamazaki
发明人： Eric S. Nordman , Miho Ozawa , Masaya Kojima , Ryoji Inaba , Yoshitaka Kodama , Motohiro Yamazaki
IPC分类号： G01N27/26 , G01N27/447
CPC分类号： G01N27/44704 , G01N27/44708 , G01N27/44782
摘要： A multi-capillary electrophoresis apparatus is provided for reducing errors during analysis caused by fluctuations in electrophoresis time among plural capillaries of the apparatus. The multi-capillary electrophoresis apparatus can contain a multi-capillary array that has a separation medium filled therein for isolating a sample. A detector component can be provided at a position remote from a sample injecting end of the array for acquiring information from the sample. A buffer container can be provided for holding a buffer solution into which the sample injecting end can be immersed. One or more temperature controlling component devices can be arranged for controlling a temperature of the buffer solution, of the detector component, or of both the buffer solution and the detector component.
摘要翻译：提供了一种多毛细管电泳装置，用于减少由于装置的多个毛细管之间的电泳时间波动引起的分析过程中的误差。多毛细管电泳装置可以包含多个毛细管阵列，其具有填充在其中的分离介质，用于分离样品。可以在远离阵列的样品注入端的位置处提供检测器部件，以从样品获取信息。可以提供缓冲容器用于保持可以浸入样品注入端的缓冲溶液。可以布置一个或多个温度控制部件装置，用于控制缓冲溶液，检测器部件或缓冲溶液和检测器部件两者的温度。

63. 发明授权

US07414118B1 Modified oligonucleotides and applications thereof 有权
标题翻译：修饰的寡核苷酸及其应用
公开(公告)号：US07414118B1
公开(公告)日：2008-08-19
申请号：US11372984
申请日：2006-03-09
申请人： Khairuzzaman Bashar Mullah , Zhaochun Ma
发明人： Khairuzzaman Bashar Mullah , Zhaochun Ma
IPC分类号： C07H21/02 , C07H21/04 , C12Q1/68 , C07H19/04
CPC分类号： C07H19/04 , C07H21/02 , C07H21/04 , C12Q1/686 , Y10T436/143333 , C12Q2525/117
摘要： Disclosed, among other things, are primers containing certain modified nucleobases in the 3′ terminal region of the primers that provide reduced formation of primer-dimers during amplification reactions, and various methods of use thereof.
摘要翻译：公开了其中包含在引物的3'末端区域中含有某些修饰的核碱基的引物，其在扩增反应期间提供缩短的引物二聚体的形成及其各种使用方法。

64. 发明授权

US07413708B2 Methods and reagents for combined PCR amplification 失效
标题翻译：组合PCR扩增的方法和试剂
公开(公告)号：US07413708B2
公开(公告)日：2008-08-19
申请号：US11775151
申请日：2007-07-09
申请人： Paul E. Mayrand
发明人： Paul E. Mayrand
IPC分类号： B01L3/00 , C07H21/04 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6818 , C07H21/04 , C12Q1/6853 , C12Q1/686 , Y10S435/81 , Y10S435/911 , C12Q2565/107 , C12Q2565/1015 , C12Q2525/186 , C12Q2525/125 , C12Q2543/101 , C12Q2537/101 , C12Q2563/113 , C12Q2531/113 , C12Q2527/101 , C12Q2521/513
摘要： An oligonucleotide probe is disclosed, the probe including an oligonucleotide, a fluorescer molecule attached to a first end of the oligonucleotide and a quencher molecule attached to the opposite end of the oligonucleotide. The probe is rendered impervious to digestion by the 5′→3′ exonuclease activity of a polymerase and the 5′→3′ extension of by a polymerase. The invention also includes methods for performing combined PCR amplification and hybridization probing, one such method including the steps of contacting a target nucleic acid sequence with PCR reagents and an oligonucleotide probe as described above, and subjecting these reagents to thermal cycling. One preferred refinement of the above method further includes the addition of a strand displacer to facilitate amplification. Additional similar combined PCR hybridization methods are disclosed, such methods not requiring probes having their 5′ ends protected, wherein (i) the polymerase lacks 5′→3′ exonuclease activity, (ii) a 5′→3′ exonuclease inhibitor is included, and (iii) an exonuclease deactivation step is performed.
摘要翻译：公开了寡核苷酸探针，所述探针包含寡核苷酸，附着于寡核苷酸第一末端的荧光分子和连接于寡核苷酸相对末端的猝灭剂分子。该探针不被聚合酶的5'→3'核酸外切酶活性消化，并且通过聚合酶的5'→3'延伸。本发明还包括用于进行组合PCR扩增和杂交探测的方法，一种这样的方法包括如下步骤：使靶核酸序列与PCR试剂和如上所述的寡核苷酸探针接触，并对这些试剂进行热循环。上述方法的一个优选的改进还包括添加链置换剂以促进扩增。公开了另外类似的组合PCR杂交方法，这种方法不需要其5'末端被保护的探针，其中（i）聚合酶缺少5'→3'核酸外切酶活性，（ii）5'→3'外切核酸酶抑制剂包括，和（iii）进行外切核酸酶失活步骤。

65. 发明授权

US6054266A Nucleic acid detection with separation 失效
标题翻译：分离核酸检测
公开(公告)号：US6054266A
公开(公告)日：2000-04-25
申请号：US276139
申请日：1988-11-23
申请人： Mel N. Kronick , Douglas H. Keith , Lincoln J. McBride , Norman M. Whiteley , Michael W. Hunkapiller
发明人： Mel N. Kronick , Douglas H. Keith , Lincoln J. McBride , Norman M. Whiteley , Michael W. Hunkapiller
IPC分类号： G01N33/50 , C12Q1/68 , G01N30/88 , G01N33/58 , C07H21/04
CPC分类号： C12Q1/6813 , C12Q1/6804 , C12Q1/6823
摘要： Nucleic acid sequences are detected by a multi-step process, involving labeling sample nucleic acid sequences, duplexing the labeled sample with a probe having a coupling element, immobilizing all of the duplexed probe and target sequence and unduplexed probe, separating specifically immobilized nucleic acid from free and non-specifically immobilized nucleic acid, releasing specifically immobilized nucleic acid, and detecting the presence of the sequence of interest by means of the label. The labeled sequence may be characterized by sizing, e.g. electrophoresis. The method provides for a sensitive and rapid means for accurate detection of sequences of interest in a wide variety of situations.
摘要翻译：通过多步法检测核酸序列，包括标记样品核酸序列，用具有偶联元件的探针双链化标记的样品，固定所有双链体探针和靶序列和未折射的探针，将特异性固定的核酸与游离和非特异性固定化的核酸，释放特异性固定化的核酸，并通过标记物检测感兴趣的序列的存在。标记序列的特征可以是尺寸，例如电泳。该方法提供用于在各种情况下精确检测感兴趣序列的敏感且快速的方法。

66. 发明授权

US5841959A Robotic interface 失效
标题翻译：机器人界面
公开(公告)号：US5841959A
公开(公告)日：1998-11-24
申请号：US598188
申请日：1994-12-06
申请人： Harry A. Guiremand
发明人： Harry A. Guiremand
IPC分类号： G06F3/14 , B25J9/16 , G01N35/00 , G05B19/042 , G05B19/409 , G06F3/033 , G06F3/048 , G06F9/06 , G06F9/44 , G06F15/00
CPC分类号： B25J9/1671 , G01N35/0092 , G05B19/0426 , G06F3/0481 , G05B2219/23258 , G05B2219/36025
摘要： An automated apparatus is programmed to perform a process by arranging a sequence of first icons on a display in the order of the process, wherein the first icons represent functions of the apparatus, and wherein at least one of the first icons provides a visual representation of a function of the apparatus. Said at least one of the first icons can be expanded to show second icons that comprise the function of said at least one of the first icons, and at least one of the second icons provides a visual representation of a subfunction of the apparatus. In a preferred mode, when said at least one of the first icons is expanded, said at least one of the first icons maintains its same sequential relationship on the display to the other of the first icons in the sequence as before it was expanded.
摘要翻译：自动化装置被编程为通过按照处理的顺序在显示器上布置一系列第一图标来执行处理，其中第一图标表示装置的功能，并且其中第一图标中的至少一个提供视觉表示该装置的功能。所述第一图标中的至少一个可以被扩展以显示构成所述至少一个第一图标的功能的第二图标，并且所述第二图标中的至少一个提供所述装置的子功能的视觉表示。在优选模式中，当所述第一图标中的至少一个被展开时，所述第一图标中的至少一个在显示器上保持与序列中的第一图标中的另一个相同的顺序关系，就像在其展开之前一样。

67. 发明授权

US5384025A Notched spacer for slab-gel electrophoresis 失效
标题翻译：板状凝胶电泳用缺口间隔物
公开(公告)号：US5384025A
公开(公告)日：1995-01-24
申请号：US206995
申请日：1994-03-07
申请人： Andrew J. Blasband
发明人： Andrew J. Blasband
IPC分类号： G01N27/447 , C25B9/00
CPC分类号： G01N27/44704
摘要： The present invention is directed to improved side spacers for use in slab gel electrophoresis that prevent the formation of channels between the electrophoresis gel and the side spacer and methods employing the side spacer. The improvement consists of forming the side spacer with one or more notches cut into the edge of the side spacer in contact with the gel, such that, when the gel hardens, the side spacer is anchored into the gel, thereby preventing the formation of channels between the side spacer and the gel.
摘要翻译：本发明涉及用于板状凝胶电泳的改进的侧面间隔物，其防止电泳凝胶和侧面间隔物之间的通道形成以及采用侧面间隔物的方法。改进之处在于，形成侧隔离物，其中一个或多个切口切割成与凝胶接触的侧间隔物的边缘，使得当凝胶硬化时，侧间隔物锚定到凝胶中，从而防止通道的形成在侧间隔物和凝胶之间。

68. 发明授权

US5374527A High resolution DNA sequencing method using low viscosity medium 失效
标题翻译：高分辨率DNA测序方法采用低粘度培养基
公开(公告)号：US5374527A
公开(公告)日：1994-12-20
申请号：US3968
申请日：1993-01-21
申请人： Paul D. Grossman
发明人： Paul D. Grossman
IPC分类号： G01N33/483 , C12M1/00 , C12N15/09 , C12Q1/68 , G01N27/447 , G01N33/50 , C12P19/34 , G01N33/00
CPC分类号： C12Q1/6869 , Y10T436/143333
摘要： A DNA sequencing method for use in sequencing a DNA target sequence up to 300 bases, preferably up to 500 bases or greater in length, by electrophoretically separating a mixture of single-stranded DNA sequencing fragments in a capillary tube. The method employs an aqueous denaturing solution comprising between about 4 and about 7 weight percent linear polyacrylamide molecules having an average molecular weight of between about 20 and about 100 kDa. The low-viscosity of the solution allows rapid loading and reloading of such solution into the capillary tube.
摘要翻译：一种DNA测序方法，用于通过在毛细管中电泳分离单链DNA测序片段的混合物，将DNA靶序列测序长达300个碱基，优选长达500个碱基或更大。该方法使用包含约4至约7重量％的平均分子量为约20至约100kDa的线性聚丙烯酰胺分子的水性变性溶液。溶液的低粘度允许将这种溶液快速加载和重新加载到毛细管中。

69. 发明授权

US5346999A Method of nucleic acid extraction 失效
标题翻译：核酸提取方法
公开(公告)号：US5346999A
公开(公告)日：1994-09-13
申请号：US328471
申请日：1989-03-24
申请人： Guy R. Cathcart , Paul D. Grossman , P. Eric Mayrand , Eric S. Nordman , Norman M. Whiteley
发明人： Guy R. Cathcart , Paul D. Grossman , P. Eric Mayrand , Eric S. Nordman , Norman M. Whiteley
IPC分类号： B01J19/00 , C12M1/00 , C12N9/06 , C12N15/10 , C40B60/14 , C07H23/00
CPC分类号： C12N9/0012 , B01J19/0046 , C12M47/06 , C12M47/12 , C12N15/1003 , B01J2219/00286 , B01J2219/00308 , B01J2219/00331 , B01J2219/00389 , B01J2219/00488 , B01J2219/00689 , B01J2219/00695 , C40B60/14 , Y10T436/25125 , Y10T436/25375
摘要： An automated apparatus is provided which implements a new method of extracting and purifying nucleic acids from cells without the use of centrifugation. In the method, a lysate is created by treating the cells with proteinase K in the presence of a lysis buffer having a high concentration of a salt. The lysate is mixed with a phenol-based solvent system, thereby creating an emulsion. The emulsion is heated to promote phase separation. Similarly, the rate of phase separation is also enhanced by increasing the surface area of the emulsion. Once the phase separation is complete, the lower organic phase is removed and the upper aqueous phase is repeatedly extracted with the phenol-based solvent a preselected number of times, and is finally extracted using chloroform. The remaining aqueous phase is then dialyzed to further purify and concentrate the nucleic acid solution. Two preferred embodiments of apparatus are presented to accomplish this extraction.
摘要翻译：提供了一种自动化装置，其实现了从细胞中提取和纯化核酸而不使用离心的新方法。在该方法中，通过在具有高浓度盐的裂解缓冲液存在下用蛋白酶K处理细胞产生裂解物。将裂解物与苯酚基溶剂体系混合，从而产生乳液。将乳液加热以促进相分离。类似地，通过增加乳液的表面积也增加了相分离速率。一旦相分离完成，除去较低的有机相，并用苯酚类溶剂预选次数反复提取上部水相，最后用氯仿萃取。然后将剩余的水相透析以进一步净化并浓缩核酸溶液。呈现装置的两个优选实施例以完成该提取。

70. 发明授权

US5298259A Subunit delivery composition and method 失效
标题翻译：亚单位配送和方法
公开(公告)号：US5298259A
公开(公告)日：1994-03-29
申请号：US788322
申请日：1991-11-05
申请人： David H. Lloyd , Robert J. DeFranco
发明人： David H. Lloyd , Robert J. DeFranco
IPC分类号： B01J19/00 , C07K1/04 , C40B40/06 , C40B40/10 , C40B60/14 , A61K9/10 , A61K9/16 , B65D3/26 , C07K1/06
CPC分类号： C07K1/045 , B01J19/0046 , B01J2219/00286 , B01J2219/00389 , B01J2219/005 , B01J2219/0059 , B01J2219/00596 , B01J2219/00686 , B01J2219/00722 , B01J2219/00725 , C40B40/06 , C40B40/10 , C40B60/14
摘要： A polymer composition having a biopolymer subunit entrapped in a swellable polymer matrix is described. The composition may be supplied in a disposable cartridge, for use in delivering a solution of the subunit in an automated biopolymer synthesis operation.
摘要翻译：描述了具有包含在可溶胀聚合物基质中的生物聚合物亚单元的聚合物组合物。组合物可以以一次性药筒供应，用于在自动生物聚合物合成操作中递送亚单位的溶液。

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