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1. 发明授权

US11754524B2 Apparatuses, systems and methods for sequencing using capillary electrophoresis 有权
公开(公告)号：US11754524B2
公开(公告)日：2023-09-12
申请号：US16922754
申请日：2020-07-07
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Ian Walton , Robert Cobene , Alexander Dukhovny , Adam Sannicandro , John Dixon , Achim Karger , Dan Kline
IPC分类号： G01N27/453 , G01N27/447 , G01N35/10
CPC分类号： G01N27/44791 , G01N27/44708 , G01N27/44721 , G01N27/44743 , G01N27/44756 , G01N35/1016 , G01N35/10
摘要： In one aspect, a biological sequencing device comprising a cartridge configured to be removed from the instrument is disclosed. In various embodiments the cartridge can include one or more capillaries suitable for capillary electrophoresis, a reservoir and a pump. In various embodiments the reservoir can contain a separation matrix. In various embodiments the pump can load a capillary with separation matrix. In another aspect the biological sequencing device can include one or more capillaries and an integrated valve assembly. In various embodiments the integrated valve assembly can provide a polymer to the one or more capillaries.

2. 发明申请

US20170038337A1 SAMPLE SEPARATION/TRANSFER DEVICE AND SAMPLE ANALYSIS METHOD 审中-公开
标题翻译：样品分离/转移装置和样品分析方法
公开(公告)号：US20170038337A1
公开(公告)日：2017-02-09
申请号：US15303554
申请日：2015-12-08
申请人： SHARP KABUSHIKI KAISHA
发明人： Shinichi GOTO , Hideki KINOSHITA
IPC分类号： G01N27/447 , G01N1/31 , G01N1/30
CPC分类号： G01N27/44739 , G01N1/30 , G01N1/31 , G01N27/44708 , G01N27/44747 , G01N2035/0487
摘要： A novel technique is provided for automatically performing the separation and transfer of analyte, and subsequent processing. A sample separation/transfer device (100) is a device for separating analyte by way of electrophoresis, dispensing the separated analyte from a dispensing part (50a) inside a buffer tank (30), and transferring the separated analyte to a transfer membrane (1) by way of causing the transfer membrane (1) to abut the dispensing part (50a) and move, in which the device includes a liquid delivery pump (11a, 11b) that replaces the liquid filling the buffer tank (30).
摘要翻译：提供了一种新技术，用于自动执行分析物的分离和转移以及随后的处理。样品分离/转移装置（100）是用于通过电泳分离分析物的装置，将分离的分析物从缓冲罐（30）内的分配部分（50a）分配，并将分离的分析物转移到转移膜（1 ）通过使转印膜（1）抵靠分注部（50a）并移动，其中该装置包括替换填充缓冲罐（30）的液体的液体输送泵（11a，11b）。

3. 发明授权

US09034163B1 Electrode for capillary electrophoresis 有权
标题翻译：毛细管电泳用电极
公开(公告)号：US09034163B1
公开(公告)日：2015-05-19
申请号：US13682533
申请日：2012-11-20
申请人： Tiansong Wang
发明人： Tiansong Wang
IPC分类号： G01N27/403 , G01N27/447
CPC分类号： G01N27/44708 , G01N27/447 , G01N27/44756
摘要： An electrode assembly for capillary electrophoresis (CE) comprises a manifold (310), a connector (305) a sheath (300), and a seal (325). A capillary tube (100) passes through the manifold, the connector, the sheath, and the seal, stopping just beyond the end of the sheath. The sheath is fillable with water (330) or another fluid that cools the capillary tube in the vicinity of the electrode, thereby preventing degradation of a sample due to heat. The sheath may be metal or plastic with a metal sleeve electrode on its exterior. The sheath is sufficiently strong to penetrate a rubber or other pierceable cap on a vial. The manifold and connector incorporate an air path (605, 312, 307) so that when the electrode is fully inserted into a vial, the contents (650) of the vial are at atmospheric pressure (or another applied pressure or vacuum).
摘要翻译：用于毛细管电泳（CE）的电极组件包括歧管（310），连接器（305），护套（300）和密封件（325）。毛细管（100）穿过歧管，连接器，护套和密封件，刚好超出护套的端部。护套可以用水（330）或在电极附近冷却毛细管的另一流体填充，从而防止样品由于热而劣化。护套可以是金属或塑料，其外部具有金属套管电极。护套足够坚固以穿透小瓶上的橡胶或其他可刺破的帽子。歧管和连接器包括空气路径（605,312,307），使得当电极完全插入小瓶中时，小瓶的内容物（650）处于大气压（或另一个施加的压力或真空）。

4. 发明授权

US07572358B2 Electrophoresis method and apparatus 失效
标题翻译：电泳法和仪器
公开(公告)号：US07572358B2
公开(公告)日：2009-08-11
申请号：US10982567
申请日：2004-11-05
申请人： Akira Wada
发明人： Akira Wada
IPC分类号： G01N33/559 , G01N27/00
CPC分类号： G01N27/44708 , G01N27/44773 , G01N27/44782
摘要： A first dimension electrophoresis apparatus of vertical type with a gel container 2 having a plurality of rectangular rod-shaped gel chambers is provided. The rod-type gels surrounded with non-conductive material plates are arranged in parallel, an upper open end of the gel container 2 is placed tightly into an upper buffer reservoir 4 and the lower open end thereof is placed tightly into a lower buffer reservoir 1. The gel container 2 and the main parts of the first buffer reservoir 4 and the lower buffer reservoir 1 are sunk in a cooling reservoir 5 filled with cooling liquid. The lower buffer reservoir has a panel portion 1b which is bent upward to penetrate the cooling reservoir. Outlet conductors thereof extend from each of the reservoirs and are insulated physically and electrically from the cooling liquid. As a result, the outer surface of the gel container can directly contact with the cooling liquid.
摘要翻译：提供了具有多个矩形棒状凝胶室的凝胶容器2的垂直型的一维电泳装置。用非导电材料板包围的棒状凝胶平行布置，凝胶容器2的上开口端紧紧地放置在上缓冲容器4中，其下开口端紧紧地放置在下缓冲储存器1中将凝胶容器2和第一缓冲容器4的主要部分以及下部缓冲容器1沉入装有冷却液的冷却容器5内。下缓冲储存器具有向上弯曲以穿透冷却储存器的面板部分1b。其出口导体从每个储存器延伸并且与冷却液物理和电气绝缘。结果，凝胶容器的外表面可以直接与冷却液接触。

5. 发明申请

US20090020429A1 ELECTROPHORESIS APPARATUS USING CAPILLARY ARRAY AND A SAMPLE TRAY 有权
标题翻译：电泳装置使用毛细管阵列和样品盘
公开(公告)号：US20090020429A1
公开(公告)日：2009-01-22
申请号：US12175998
申请日：2008-07-18
申请人： Miho Ozawa , Ryoji Inaba , Tomohiro Shoji , Manabu Akiba
发明人： Miho Ozawa , Ryoji Inaba , Tomohiro Shoji , Manabu Akiba
IPC分类号： G01N27/00
CPC分类号： G01N27/44708
摘要： Even if a standby time to the measurement becomes long, a sample in a sample container is prevented from deteriorating. A cooling mechanism is provided in a sample tray for holding a sample plate, or in a plate-mounting portion of a table-type auto sampler of a capillary electrophoresis apparatus, so that the sample plate is cooled during the measurement also.
摘要翻译：即使对测量的待机时间变长，也能够防止样本容器内的样品劣化。在用于保持样品板的样品托盘中或在毛细管电泳装置的台式自动取样器的板安装部分中设置冷却机构，使得样品板在测量期间也被冷却。

6. 发明授权

US07316770B2 Method of electrophoresis, electrophoresis apparatus and capillary array 有权
标题翻译：电泳方法，电泳装置和毛细管阵列
公开(公告)号：US07316770B2
公开(公告)日：2008-01-08
申请号：US09814860
申请日：2001-03-23
申请人： Ryoji Inaba , Tomoyuki Sakai , Satoshi Takahashi , Muneo Maeshima , Miho Ozawa , Masaya Kojima , Daizo Tokinaga
发明人： Ryoji Inaba , Tomoyuki Sakai , Satoshi Takahashi , Muneo Maeshima , Miho Ozawa , Masaya Kojima , Daizo Tokinaga
IPC分类号： G01N27/26
CPC分类号： G01N27/44708 , G01N27/44782
摘要： A method of electrophoresis, an electrophoresis apparatus and a capillary array in which a fluorescently labelled sample is supplied into capillaries in a capillary array constituted by a plurality of capillaries and including a sample supply portion, an electrophoresis medium supply portion and a detection portion while controlling the temperature of the capillary array through gas circulation, the sample is caused to migrate and separated in the capillaries through electrophoresis, and at the region where the capillaries are contacted or come close each other a plurality of capillaries are contacted to a solid body so as to dissipate heat generated from the capillaries to the solid body.
摘要翻译：一种电泳方法，电泳装置和毛细管阵列，其中荧光标记的样品被供应到由多个毛细管构成的毛细管阵列的毛细管中，并且包括样品供应部分，电泳介质供应部分和检测部分，同时控制通过气体循环的毛细管阵列的温度，通过电泳使样品在毛细管中迁移和分离，并且在毛细管接触或彼此靠近的区域，多个毛细管与固体接触，以便以将从毛细管产生的热量散发到固体。

7. 发明申请

US20060219559A1 Capillary array and capillary electrophoresis apparatus 有权
公开(公告)号：US20060219559A1
公开(公告)日：2006-10-05
申请号：US11395158
申请日：2006-04-03
申请人： Seiichi Ugai , Tomonari Morioka , Ryoji Inaba , Akihiro Suzuki , Yoshinori Ookoshi , Yoshiyuki Okishima , Tsukasa Ohira , Motohiro Yamazaki , Takashi Gomi
发明人： Seiichi Ugai , Tomonari Morioka , Ryoji Inaba , Akihiro Suzuki , Yoshinori Ookoshi , Yoshiyuki Okishima , Tsukasa Ohira , Motohiro Yamazaki , Takashi Gomi
IPC分类号： G01N27/00
CPC分类号： G01N27/44708
摘要： In a case where the temperature of capillaries cannot be controlled rapidly in accordance with the change of the amount of heat generated from the capillaries, the phoretic speed of the sample is not controlled constant to lower the analyzing performance for the sample. This invention intends to stabilize the electrophoretic speed. The surface of a substrate on which a plurality of capillaries are arranged is in contact with a member capable of temperature control thereby controlling the temperature of the capillaries. Since a member capable of controlling the temperature and the capillaries are in direct contact with each other according to the invention, the temperature of the capillary can be controlled rapidly at a high accuracy, so that electrophoretic speed is stabilized.

8. 发明申请

US20050139533A1 Electrophoretic separation system 审中-公开
标题翻译：电泳分离系统
公开(公告)号：US20050139533A1
公开(公告)日：2005-06-30
申请号：US10504963
申请日：2003-02-12
申请人： Kevin Auton , Sergei Schurov , Paul Ryan
发明人： Kevin Auton , Sergei Schurov , Paul Ryan
IPC分类号： G01N27/447 , B01D61/42
CPC分类号： G01N27/44773 , G01N27/44708 , G01N27/4476
摘要： An electrophoresis device for use in separating a mixture of analytes in a fluid sample is provided, the device comprising a first dimension separation medium through which the analytes may migrate, the separation medium being carried on one face of a flexible sheet, the surface area of that face being greater than that of the region of contact between the separation medium and the sheet, wherein the first dimension separation medium is located within a first separation zone which is defined at least partly by the flexible sheet, the device further comprising a fluid chamber, separated from the first dimension separation zone by the flexible sheet, in which fluid may be retained in contact with that face of the sheet opposite to the face on which the separation medium is carried. The device may additionally comprise a second separation zone, in which a second separation medium may be located, in order to carry out a second dimension electrophoretic separation.
摘要翻译：提供了一种用于分离流体样品中分析物混合物的电泳装置，该装置包括第一维分离介质，分析物可以通过其移动，分离介质承载在柔性片的一个面上，表面积所述表面大于所述分离介质和所述片材之间的接触区域的面，其中所述第一尺寸分离介质位于至少部分地由所述柔性片材限定的第一分离区域内，所述装置还包括流体室通过柔性片与第一尺寸分离区分离，其中流体可以保持与片材的与分离介质携带的面相反的面的接触面。该装置可以另外包括第二分离区，其中可以定位第二分离介质，以便执行第二维电泳分离。

9. 发明申请

US20040084402A1 Polymeric microfabricated fluidic device suitable for ultraviolet detection 失效
标题翻译：适用于紫外线检测的聚合物微制流体装置
公开(公告)号：US20040084402A1
公开(公告)日：2004-05-06
申请号：US10380608
申请日：2003-03-13
发明人： James William Ashmead , Sau Lan Tang
IPC分类号： G11B005/127
CPC分类号： G01N30/6095 , B01L3/5027 , B01L3/502707 , B01L3/50273 , B01L7/00 , B01L2200/0689 , B01L2200/12 , B01L2300/0816 , B01L2300/0887 , B01L2300/185 , B01L2300/1894 , B01L2400/0418 , G01N21/05 , G01N21/33 , G01N27/44708 , G01N27/44721 , G01N27/44791 , G01N2021/0346 , G01N2030/3007 , G01N30/74
摘要： A polymer-based microfluidic device and its manufacturing methods. This microfluidic device is suitable for chromatographic and electrophoretic separations in which the detection of the components in the fluid is by means of ultraviolet (UV) spectroscopy. The device further comprises at least one cooling channel adjacent the separation channel, said cooling channel conducting a cooling fluid through the device for removing heat from the the separation channel. At least one section of the separation channel has an increased cross-sectional dimension with respect to other sections of said channel to provide an increased optical path length when the device is used in an ultraviolet detection arrangement.
摘要翻译：一种基于聚合物的微流体装置及其制造方法。该微流体装置适用于通过紫外（UV）光谱法检测流体中的组分的色谱和电泳分离。所述装置还包括与所述分离通道相邻的至少一个冷却通道，所述冷却通道通过所述装置传导冷却流体，以从所述分离通道移除热量。分离通道的至少一个部分相对于所述通道的其它部分具有增加的横截面尺寸，以在该装置用于紫外线检测装置时提供增加的光程长度。

10. 发明申请

US20030116438A1 Multi-capillary electrophoresis apparatus 有权
公开(公告)号：US20030116438A1
公开(公告)日：2003-06-26
申请号：US10252557
申请日：2002-09-24
发明人： Motohiro Yamazaki , Masaya Kojima , Ryoji Inaba
IPC分类号： G01N027/26 , G01N027/447
CPC分类号： G01N27/44782 , G01N27/44708
摘要： Scattering on analysis in a multi-capillary electrophoresis apparatus is suppressed. The multi-capillary electrophoresis apparatus has a multi-capillary array that has a sample and a separation medium for separating a sample charged therein, and has a sampling section formed at ends of the capillaries and a detector part for acquiring information depending on the sample thus separated, a means for applying a voltage between the sampling section and the detector part, a chamber part having an air blowing mechanism and a temperature controlling mechanism, a capillary housing base provided on a leeward side of the air blowing mechanism and housing the multi-capillary array, and a first straightening plate provided between the chamber part and the capillary housing base.

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