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1. 发明申请

US20110287431A1 MODIFIED OLIGONUCLEOTIDES AND APPLICATIONS THEREOF 有权
标题翻译：修改后的寡核苷酸及其应用
公开(公告)号：US20110287431A1
公开(公告)日：2011-11-24
申请号：US13052382
申请日：2011-03-21
申请人： Khairuzzaman Bashar MULLAH , Zhaochun Ma , Wanli Bi
发明人： Khairuzzaman Bashar MULLAH , Zhaochun Ma , Wanli Bi
IPC分类号： C12Q1/68 , G01N33/559 , G01N27/447
CPC分类号： C07H21/04 , C07H21/02 , C12Q1/6848 , C12Q2525/117
摘要： Disclosed, among other things, are primers containing certain modified nucleobases in the 3′ terminal region of the primers that provide reduced formation of primer-dimers during amplification reactions, and various methods of use thereof.
摘要翻译：公开了其中包含在引物的3'末端区域中含有某些修饰的核碱基的引物，其在扩增反应期间提供缩短的引物二聚体的形成及其各种使用方法。

2. 发明授权

US07517978B1 Modified oligonucleotides and applications thereof 有权
标题翻译：修饰的寡核苷酸及其应用
公开(公告)号：US07517978B1
公开(公告)日：2009-04-14
申请号：US11250192
申请日：2005-10-12
申请人： Khairuzzaman Bashar Mullah , Zhaochun Ma , Wanli Bi
发明人： Khairuzzaman Bashar Mullah , Zhaochun Ma , Wanli Bi
IPC分类号： C07H21/04 , C07H21/02 , C12Q1/68 , C12P19/34
CPC分类号： C07H21/04 , C07H21/02 , C12Q1/6848 , C12Q2525/117
摘要： Disclosed, among other things, are primers containing certain modified nucleobases in the 3′ terminal region of the primers that provide reduced formation of primer-dimers during amplification reactions, and various methods of use thereof.
摘要翻译：公开了其中包含在引物的3'末端区域中含有某些修饰的核碱基的引物，其在扩增反应期间提供缩短的引物二聚体的形成及其各种使用方法。

3. 发明授权

US07943752B2 Modified oligonucleotides and applications thereof 有权
标题翻译：修饰的寡核苷酸及其应用
公开(公告)号：US07943752B2
公开(公告)日：2011-05-17
申请号：US12423753
申请日：2009-04-14
申请人： Khairuzzaman Bashar Mullah , Zhaochun Ma , Wanli Bi
发明人： Khairuzzaman Bashar Mullah , Zhaochun Ma , Wanli Bi
IPC分类号： C07H21/02 , C07H21/04 , C12Q1/68 , C12P19/34
CPC分类号： C07H21/04 , C07H21/02 , C12Q1/6848 , C12Q2525/117
摘要： Disclosed, among other things, are primers containing certain modified nucleobases in the 3′ terminal region of the primers that provide reduced formation of primer-dimers during amplification reactions, and various methods of use thereof.
摘要翻译：公开了其中包含在引物的3'末端区域中含有某些修饰的核碱基的引物，其在扩增反应期间提供缩短的引物二聚体的形成及其各种使用方法。

4. 发明授权

US07408051B2 Modified oligonucleotides and applications thereof 有权
公开(公告)号：US07408051B2
公开(公告)日：2008-08-05
申请号：US11106044
申请日：2005-04-14
申请人： Zhaochun Ma , Khairuzzaman Bashar Mullah
发明人： Zhaochun Ma , Khairuzzaman Bashar Mullah
IPC分类号： C07H21/02 , C07H21/04 , C07H19/04 , C12Q1/68
CPC分类号： C12Q1/6848 , C07H21/04 , C12Q1/6853 , C12Q2525/117
摘要： Disclosed, among other things, are primers containing certain modified nucleobases in the 3′ terminal region of the primers that provide reduced formation of primer-dimers during amplification reactions, and various methods of use thereof.

5. 发明授权

US06821727B1 Hybridization assay using self-quenching fluorescence probe 失效
标题翻译：使用自熄荧光探针的杂交测定
公开(公告)号：US06821727B1
公开(公告)日：2004-11-23
申请号：US09627753
申请日：2000-07-28
申请人： Kenneth J. Livak , Susan J. A. Flood , Jeffrey Mamoro , Khairuzzaman Bashar Mullah
发明人： Kenneth J. Livak , Susan J. A. Flood , Jeffrey Mamoro , Khairuzzaman Bashar Mullah
IPC分类号： C12Q168
CPC分类号： C12Q1/6818 , C12Q1/6834 , Y10S977/924 , C12Q2525/301 , C12Q2565/1015 , C12Q2565/107 , C12Q2561/101
摘要： A hybridization assay is provided which uses an oligonucleotide probe which includes a fluorescent reporter molecule and a quencher molecule capable of quenching the fluorescence of the reporter molecule. The oligonucleotide probe is constructed such that the probe exists in at least one single-stranded confirmation when unhybridized where the quencher molecule is near enough to the reporter molecule to quench the fluorescence of the reporter molecule. The oligonucleotide probe also exists in at least one conformation when hybridized to a target polynucleotide where the quencher molecule is not positioned close enough to the reporter molecule to quench the fluorescence of the reporter molecule. By adopting these hybridized and unhybridized conformations, the reporter molecule and quencher molecule on the probe exhibits different fluorescence signal intensities when the probe is hybridized and unhybridized. As a result, it is possible to determine whether the probe is hybridized or unhybridized based on a change in the fluorescence intensity of the reporter molecule, the quencher molecule, or a combination thereof. In addition, because the probe can be designed such that the quencher molecule quenches the reporter molecule when the probe is not hybridized, the probe can be designed such that the reporter molecule exhibits limited fluorescence until the probe is either hybridized or digested.
摘要翻译：提供了使用寡核苷酸探针的杂交测定法，其包括荧光报告分子和能够猝灭报告分子荧光的猝灭剂分子。构建寡核苷酸探针，使得探针在未杂交的情况下以至少一种单链确认存在，其中淬灭剂分子足够接近报道分子以淬灭报道分子的荧光。当寡核苷酸探针与靶多核苷酸杂交时，寡核苷酸探针也存在至少一个构象，其中猝灭剂分子不能与报道分子紧密接近以猝灭报道分子的荧光。通过采用这些杂交和非杂交构象，当探针杂交和未杂交时，探针上的报道分子和猝灭剂分子表现出不同的荧光信号强度。结果，可以基于报道分子，猝灭剂分子或其组合的荧光强度的变化来确定探针是杂交还是未杂交。此外，因为探针可以被设计成使得当探针不杂交时，猝灭剂分子淬灭报告分子，所以探针可被设计成使得报告分子在荧光探针杂交或消化之前显示有限的荧光。

6. 发明授权

US5723591A Self-quenching fluorescence probe 失效
标题翻译：自熄荧光探针
公开(公告)号：US5723591A
公开(公告)日：1998-03-03
申请号：US559405
申请日：1995-11-15
申请人： Kenneth J. Livak , Susan J.A. Flood , Jeffrey Marmaro , Khairuzzaman Bashar Mullah
发明人： Kenneth J. Livak , Susan J.A. Flood , Jeffrey Marmaro , Khairuzzaman Bashar Mullah
IPC分类号： C12N15/09 , C07H21/00 , C12N15/00 , C12Q1/68 , C07H19/00 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6818 , C12Q1/6834 , C12Q1/6837
摘要： An oligonucleotide probe is provided which includes a fluorescent reporter molecule and a quencher molecule capable of quenching the fluorescence of the reporter molecule. The oligonucleotide probe is constructed such that the probe exists in at least one single-stranded conformation when unhybridized where the quencher molecule is near enough to the reporter molecule to quench the fluorescence of the reporter molecule. The oligonucleotide probe also exists in at least one conformation when hybridized to a target polynucleotide where the quencher molecule is not positioned close enough to the reporter molecule to quench the fluorescence of the reporter molecule. By adopting these hybridized and unhybridized conformations, the reporter molecule and quencher molecule on the probe exhibit different fluorescence signal intensities when the probe is hybridized and unhybridized. As a result, it is possible to determine whether the probe is hybridized or unhybridized based on a change in the fluorescence intensity of the reporter molecule, the quencher molecule, or a combination thereof. In addition, because the probe can be designed such that the quencher molecule quenches the reporter molecule when the probe is not hybridized, the probe can be designed such that the reporter molecule exhibits limited fluorescence until the probe is either hybridized or digested.
摘要翻译：提供了一种寡核苷酸探针，其包括荧光报道分子和能够猝灭报道分子荧光的猝灭剂分子。构建寡核苷酸探针，使得探针在未杂交的情况下以至少一个单链构象存在，其中猝灭剂分子足够接近报道分子以淬灭报道分子的荧光。当寡核苷酸探针与靶多核苷酸杂交时，寡核苷酸探针也存在至少一个构象，其中猝灭剂分子不能与报道分子紧密接近以猝灭报道分子的荧光。通过采用这些杂交和非杂交构象，当探针杂交和未杂交时，探针上的报道分子和猝灭剂分子表现出不同的荧光信号强度。结果，可以基于报道分子，猝灭剂分子或其组合的荧光强度的变化来确定探针是杂交还是未杂交。此外，因为探针可以被设计成使得当探针不杂交时，猝灭剂分子淬灭报告分子，所以探针可被设计成使得报告分子在荧光探针杂交或消化之前显示有限的荧光。

7. 发明授权

US08658776B2 Synthesis of 2′,3′-dideoxynucleosides for automated DNA synthesis and pyrophosphorolysis activated polymerization 有权
标题翻译：用于自动DNA合成和焦磷酸裂解活化聚合的2'，3'-双脱氧核苷合成
公开(公告)号：US08658776B2
公开(公告)日：2014-02-25
申请号：US13117029
申请日：2011-05-26
申请人： Zhaochun Ma , Khairuzzaman Bashar Mullah , Robert G. Eason
发明人： Zhaochun Ma , Khairuzzaman Bashar Mullah , Robert G. Eason
IPC分类号： C07H19/06 , C07H21/00 , C07H21/02 , C07H21/04 , C07D239/54
CPC分类号： C07H19/16 , C07D239/54 , C07D405/04 , C07D473/18 , C07D473/34 , C07H19/06 , C07H21/00 , C07H21/02 , C07H21/04 , C12Q1/6806
摘要： Methods for preparation of 2′,3′-dideoxynucleotides support structures, such as 2′,3′-dideoxyguanosine, 2′,3′-dideoxyadenosine, and 3′-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated DNA synthesis and pyrophosphorolysis activated polymerization.
摘要翻译：公开了制备2'，3'-二脱氧核苷酸支持结构的方法，例如2'，3'-二脱氧鸟苷，2'，3'-二脱氧腺苷和3'-脱氧胸苷支持结构。还提供了使用这些结构的各种方法，例如其用于自动DNA合成和焦磷酸裂解活化聚合的用途。

8. 发明授权

US08637247B2 Methods of detecting target nucleic acids 有权
标题翻译：检测靶核酸的方法
公开(公告)号：US08637247B2
公开(公告)日：2014-01-28
申请号：US13590794
申请日：2012-08-21
申请人： Muhammad Sharaf , Timothy Woudenberg , Khairuzzaman Bashar Mullah
发明人： Muhammad Sharaf , Timothy Woudenberg , Khairuzzaman Bashar Mullah
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6825 , C12Q1/6816 , C12Q1/6823 , C12Q1/6827 , C12Q1/6832 , C12Q1/6876 , G01N27/44726 , G01N27/44791 , C12Q2565/631
摘要： The present disclosure relates to methods of identifying target nucleic acids by using coded molecules and its analysis by translocation through a nanopore. Generally, coded molecules are subject to a target polynucleotide dependent modification. The modified coded molecule is detected by isolating the modified coded molecules from the unmodified coded molecules prior to analysis through the nanopore or by detecting a change in the signal pattern of the coded molecule when analyzed through the nanopore.
摘要翻译：本公开内容涉及通过使用编码分子鉴定靶核酸的方法及其通过纳米孔易位的分析。通常，经编码的分子经受目标多核苷酸依赖性修饰。通过在通过纳米孔分析之前将经修饰的编码分子与未修饰的编码分子分离，或者当通过纳米孔分析时，通过检测编码分子的信号图谱的变化来检测修饰的编码分子。

9. 发明申请

US20100267938A1 USE OF ANTIBODY-SURROGATE ANTIGEN SYSTEMS FOR DETECTION OF ANALYTES 审中-公开
标题翻译：用于检测分析物的抗体表位抗原系统的应用
公开(公告)号：US20100267938A1
公开(公告)日：2010-10-21
申请号：US12705746
申请日：2010-02-15
申请人： Steven M. Menchen , Joe Y. L. Lam , Zhaochun Ma , Khairuzzaman Bashar Mullah
发明人： Steven M. Menchen , Joe Y. L. Lam , Zhaochun Ma , Khairuzzaman Bashar Mullah
IPC分类号： C07K16/00 , C07F7/02 , C07K1/00 , C07D473/00
CPC分类号： G01N33/582 , C07D311/88 , G01N33/532 , G01N33/58
摘要： Compositions, methods and kits for labeling proteins and uses in reporter systems for detecting, quantifying and/or characterizing analytes.
摘要翻译：用于标记蛋白质的组合物，方法和试剂盒以及报告系统中用于检测，定量和/或表征分析物的用途。

10. 发明授权

US06258569B1 Hybridization assay using self-quenching fluorescence probe 有权
标题翻译：使用自熄荧光探针的杂交测定
公开(公告)号：US06258569B1
公开(公告)日：2001-07-10
申请号：US09436454
申请日：1999-11-08
申请人： Kenneth J. Livak , Susan J. A. Flood , Jeffrey Mamoro , Khairuzzaman Bashar Mullah
发明人： Kenneth J. Livak , Susan J. A. Flood , Jeffrey Mamoro , Khairuzzaman Bashar Mullah
IPC分类号： C12N100
CPC分类号： C12Q1/6818 , C12Q1/6834 , C12Q1/6837 , C12Q2565/519 , C12Q2563/107 , C12Q2561/101 , C12Q2525/185 , C12Q2525/301 , C12Q2565/1015 , C12Q2565/107
摘要： Provided is a method of nucleic acid amplification. In one embodiment, the method comprises performing nucleic acid amplification on a target polynucleotide using a nucleic acid polymerase having 5′ to 3′ nuclease activity, a primer capable of hybridizing to the target polynucleotide, and an oligonucleotide probe under amplification conditions such that the probe hybridizes to the target polynucleotide 3′ relative to the primer and the probe does not hybridize with itself to form a hairpin structure. The oligonucleotide probe has at one end a fluorescent reporter and at the other end a quencher that quenches the fluorescence of the reporter molecule when both the fluorescent reporter and quencher are attached to the probe. Digestion of the oligonucleotide probe by the polymerase during amplification is effective to separate the reporter from the quencher, whereby a fluorescence signal of the reporter is increased.
摘要翻译：提供了核酸扩增的方法。在一个实施方案中，所述方法包括使用具有5'至3'核酸酶活性的核酸聚合酶在靶多核苷酸上进行核酸扩增，能够与靶多核苷酸杂交的引物和寡核苷酸探针在扩增条件下进行，使得探针与靶多核苷酸3'相对于引物杂交，并且探针不与其自身杂交以形成发夹结构。寡核苷酸探针在一端具有荧光报告基团，另一端是当荧光报道分子和猝灭剂连接到探针上时淬灭报道分子的荧光的猝灭剂。在扩增期间通过聚合酶消化寡核苷酸探针有效地将报道分子从猝灭剂中分离出来，从而增加了报道分子的荧光信号。

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