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41. 发明授权

US08604243B2 Fluorescent ion indicators for cadmium and lanthanide ion detection 有权
标题翻译：用于镉和镧系元素离子检测的荧光离子指示剂
公开(公告)号：US08604243B2
公开(公告)日：2013-12-10
申请号：US12158641
申请日：2006-12-20
申请人： Kyle Gee , Vladimir Martin
发明人： Kyle Gee , Vladimir Martin
IPC分类号： C07C211/00 , C07D311/82
CPC分类号： C07D491/22 , C07D311/90
摘要： The present invention provides a metal chelator and methods that facilitate binding, detecting, monitoring and quantitating of heavy metal ions in a sample. This metal chelating moiety has the following formula
摘要翻译：本发明提供金属螯合剂和促进样品中重金属离子结合，检测，监测和定量的方法。该金属螯合部分具有下式

42. 发明申请

US20130287689A1 Modified Hydrocyanine Dyes for the Detection of Reactive Oxygen Species 审中-公开
标题翻译：用于检测活性氧物质的改性水解染料
公开(公告)号：US20130287689A1
公开(公告)日：2013-10-31
申请号：US13881672
申请日：2011-11-01
申请人： Hee Chol Kang , Kyle Gee , Bhaskar Mandavilli , Lai-Qiang Ying , Bruce Branchaud
发明人： Hee Chol Kang , Kyle Gee , Bhaskar Mandavilli , Lai-Qiang Ying , Bruce Branchaud
IPC分类号： G01N21/64 , C09B57/00
CPC分类号： G01N21/6486 , C09B23/06 , C09B23/08 , C09B23/083 , C09B57/00
摘要： The present invention is directed to compounds, compositions, methods, and kits for detecting reactive oxygen species (ROS) by conventional fluorescence microscopy, fluorescence spectroscopy, flow cytometry, and/or high content imaging. The compounds disclosed herein are novel reduced dyes, including Cy-based hydrocyanine dyes and Cy-based deuterocyanine dyes, which dyes are probes for detecting ROS and measuring oxidative stress in cells either in vitro and/or in vivo. Also described herein are processes for preparing novel reduced dyes, i.e., ROS probes, for use in the disclosed compositions, methods and kits.
摘要翻译：本发明涉及通过常规荧光显微镜，荧光光谱，流式细胞术和/或高含量成像检测活性氧（ROS）的化合物，组合物，方法和试剂盒。本文公开的化合物是新的还原染料，包括基于Cy的氢化花青染料和Cy-基氘异氰染料，其染料是用于检测ROS的探针和测量体外和/或体内细胞中的氧化应激。本文还描述了用于制备用于所公开的组合物，方法和试剂盒的新型还原染料即ROS探针的方法。

43. 发明授权

US08318502B2 Long wavelength fluorogenic intracellular ion indicators that are well retained in the cytosol 有权
标题翻译：长波长的荧光细胞内离子指示剂，很好地保留在细胞溶质中
公开(公告)号：US08318502B2
公开(公告)日：2012-11-27
申请号：US12602973
申请日：2008-06-05
申请人： Kyle Gee , Vladimir Martin
发明人： Kyle Gee , Vladimir Martin
IPC分类号： G01N33/50 , C07D273/01 , C07D311/90 , C07D491/147
CPC分类号： G01N33/84 , A61K31/353 , C07D311/90 , C07D413/04 , C07D413/10 , C07D491/04 , C07D491/147 , C09B11/24 , G01N33/582 , G01N33/6872 , G01N2223/406 , G01N2458/00
摘要： Cell permeable metal ion indicator compounds and methods of their use and synthesis are described. The compound comprises a metal chelating moiety (Mc), a reporter molecule and two or more lipophilic groups (GL) covalently bonded through a linker to the reporter molecule, wherein the lipophilic groups, when present in a live cell, are cleaved resulting in two or more negatively charged groups.
摘要翻译：描述了细胞可渗透金属离子指示剂化合物及其使用和合成方法。化合物包括金属螯合部分（Mc），报告分子和通过接头共价键合到报告分子上的两个或多个亲油基团（GL），其中亲和基团当存在于活细胞中时被切割，导致两个或更多带负电的组。

44. 发明授权

US07989617B2 Crown ether derivatives 有权
标题翻译：冠醚衍生物
公开(公告)号：US07989617B2
公开(公告)日：2011-08-02
申请号：US12546179
申请日：2009-08-24
申请人： Kyle Gee , Vladimir Martin
发明人： Kyle Gee , Vladimir Martin
IPC分类号： C07D225/00 , C07D267/22
CPC分类号： C07D273/00 , C07D413/04 , C07D413/14
摘要： The invention describes crown ether chelators, including crown ethers having the formula:
摘要翻译：本发明描述了冠醚螯合剂，包括具有下式的冠醚：

45. 发明申请

US20110021372A1 Compositions and methods for detection and isolation of phosphorylated molecules 审中-公开
标题翻译：用于磷酸化分子检测和分离的组合物和方法
公开(公告)号：US20110021372A1
公开(公告)日：2011-01-27
申请号：US12821003
申请日：2010-06-22
申请人： Brian AGNEW , Joseph Beechem , Kyle Gee , Richard Haugland , Thomas Steinberg , Wayne Patton
发明人： Brian AGNEW , Joseph Beechem , Kyle Gee , Richard Haugland , Thomas Steinberg , Wayne Patton
IPC分类号： C40B30/04 , C12Q1/68 , G01N33/68 , G01N33/566
CPC分类号： G01N33/5008 , C12Q1/42 , C12Q1/485 , G01N33/502 , G01N33/5091 , G01N2333/4709 , Y10T436/142222 , Y10T436/145555 , Y10T436/147777 , Y10T436/201666
摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
摘要翻译：本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在磷酸化结合化合物和导致三元复合物的磷酸化靶分子。本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。

46. 发明申请

US20100068748A1 Fluorescent Ion Indicators for Cadmium and Lanthanide Ion Detection 有权
标题翻译：用于镉和镧系元素离子检测的荧光离子指示剂
公开(公告)号：US20100068748A1
公开(公告)日：2010-03-18
申请号：US12158641
申请日：2006-12-20
申请人： Kyle Gee , Vladimir Martin
发明人： Kyle Gee , Vladimir Martin
IPC分类号： C12Q1/02 , C07D311/86
CPC分类号： C07D491/22 , C07D311/90
摘要： The present invention provides a metal chelator and methods that facilitate binding, detecting, monitoring and quantitating of heavy metal ions in a sample. This metal chelating moiety has the following formula
摘要翻译：本发明提供金属螯合剂和促进样品中重金属离子结合，检测，监测和定量的方法。该金属螯合部分具有下式

47. 发明申请

US20080044804A1 COMPOSITIONS AND METHODS FOR DETECTION AND ISOLATION OF PHOSPHORYLATED MOLECULES 审中-公开
标题翻译：用于检测和分离磷酸化分子的组合物和方法
公开(公告)号：US20080044804A1
公开(公告)日：2008-02-21
申请号：US11697103
申请日：2007-04-05
申请人： Brian AGNEW , Joseph Beechem , Kyle Gee , Richard Haugland , Thomas Steinberg , Wayne Patton
发明人： Brian AGNEW , Joseph Beechem , Kyle Gee , Richard Haugland , Thomas Steinberg , Wayne Patton
IPC分类号： G01N33/00 , C12Q1/00 , G01N33/20
CPC分类号： G01N33/5008 , C12Q1/42 , C12Q1/485 , G01N33/502 , G01N33/5091 , G01N2333/4709 , Y10T436/142222 , Y10T436/145555 , Y10T436/147777 , Y10T436/201666
摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
摘要翻译：本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在磷酸化结合化合物和导致三元复合物的磷酸化靶分子。本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。

48. 发明申请

US20070161112A1 HEAVY METAL BINDING COMPOUNDS AND THEIR METHOD OF USE 有权
标题翻译：重金属结合化合物及其使用方法
公开(公告)号：US20070161112A1
公开(公告)日：2007-07-12
申请号：US11622900
申请日：2007-01-12
申请人： Kyle Gee , Jolene Bradford , Vladimir Martin
发明人： Kyle Gee , Jolene Bradford , Vladimir Martin
IPC分类号： G01N33/20 , C07D311/88 , C07D263/08 , C07D209/04 , C07D413/04
CPC分类号： C07D413/04 , C07D209/14 , C07D335/12 , C09B11/28
摘要： The present invention provides a metal chelator and methods that facilitate binding, detecting, monitoring and quantitating of heavy metal ions in a sample. This metal chelating moiety is a —N,N,O-triacetic acid analog of BAPTA and has the following formula
摘要翻译：本发明提供金属螯合剂和促进样品中重金属离子结合，检测，监测和定量的方法。该金属螯合部分是BAPTA的-N，N，O-三乙酸类似物，具有下式

49. 发明申请

US20070099222A1 Methods and compositions for labeling nucleic acids 有权
标题翻译：用于标记核酸的方法和组合物
公开(公告)号：US20070099222A1
公开(公告)日：2007-05-03
申请号：US11588697
申请日：2006-10-27
申请人： Kyle Gee , Brian Agnew
发明人： Kyle Gee , Brian Agnew
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6806 , C12Q1/68 , C12Q1/6816 , C12Q1/6841 , G01N33/5011 , G01N33/582 , C12Q2525/101 , C12Q2563/107
摘要： The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation. The methods of the invention can be used in a wide variety of applications including clinical diagnosis of diseases and disorders in which cellular proliferation is involved, toxicity assays, and as a tool for the study of chromosomes' ultrastructures.
摘要翻译：本发明涉及体外和体内标记核酸聚合物的方法。提供了包括掺入核酸聚合物的核苷酸类似物与附着于标记物上的试剂之间的[3 + 2]环加成的某些方法。提供了包括掺入核酸聚合物的核苷酸类似物与包含连接到标签上的取代的三芳基膦的试剂之间的施陶丁格连接的其它方法。这种方法不需要固定和变性，因此可以应用于活细胞和生物体中核酸聚合物的标记。还提供了测量细胞增殖的方法。在这些方法中，测量结合到DNA中的标记量作为细胞增殖的指示。本发明的方法可用于各种应用，包括涉及细胞增殖的疾病和病症的临床诊断，毒性测定以及用于研究染色体超微结构的工具。

50. 发明授权

US07169922B2 Derivatives of 1,2-dihydro-7-hydroxyquinolines containing fused rings 有权
标题翻译：含稠环的1,2-二氢-7-羟基喹啉的衍生物
公开(公告)号：US07169922B2
公开(公告)日：2007-01-30
申请号：US10713670
申请日：2003-11-13
申请人： Zhenjun Diwu , Jixiang Liu , Kyle Gee
发明人： Zhenjun Diwu , Jixiang Liu , Kyle Gee
IPC分类号： C07D471/04 , C07D491/04 , C07D495/04 , C07D495/14 , C07D495/22
CPC分类号： G01N33/582 , C07D471/04 , C07D491/04 , C07D491/044 , C07D491/14 , C07D491/147 , C07D491/22 , C07D495/04 , C07D495/14 , C07D495/22 , C07D498/14 , C12Q1/6816 , Y10S436/80 , Y10T436/141111 , Y10T436/143333 , Y10T436/145555 , C12Q2563/107
摘要： The present invention describes novel dyes, including coumarins, rhodamines, and rhodols that incorporate additional fused aromatic rings. The dyes of the invention absorb at a longer wavelength than structurally similar dyes that do not possess the fused aromatic rings. Many of the dyes of the invention are useful fluorescent dyes. The invention includes chemically reactive dyes, dye-conjugates, and the use of such dyes in staining samples and detecting ligands or other analytes.
摘要翻译：本发明描述了新颖的染料，包括掺入另外的稠合芳环的香豆素，罗丹明和罗丹醇。本发明的染料比不具有稠合芳环的结构相似的染料长的波长吸收。本发明的许多染料是有用的荧光染料。本发明包括化学活性染料，染料共轭物，以及这些染料在染色样品和检测配体或其他分析物中的用途。

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