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1. 发明授权

US08680320B2 Heavy metal binding compounds and their method of use 有权
标题翻译：重金属结合化合物及其使用方法
公开(公告)号：US08680320B2
公开(公告)日：2014-03-25
申请号：US12636687
申请日：2009-12-11
申请人： Kyle Gee , Jolene Bradford , Vladimir Martin
发明人： Kyle Gee , Jolene Bradford , Vladimir Martin
IPC分类号： C07C229/00 , C07C321/00
CPC分类号： C07D413/04 , C07D209/14 , C07D335/12 , C09B11/28
摘要： The present invention provides a metal chelator and methods that facilitate binding, detecting, monitoring and quantitating of heavy metal ions in a sample. This metal chelating moiety is a —N,N,O-triacetic acid analog of BAPTA and has the following formula
摘要翻译：本发明提供金属螯合剂和促进样品中重金属离子结合，检测，监测和定量的方法。该金属螯合部分是BAPTA的-N，N，O-三乙酸类似物，具有下式

2. 发明申请

US20070161112A1 HEAVY METAL BINDING COMPOUNDS AND THEIR METHOD OF USE 有权
标题翻译：重金属结合化合物及其使用方法
公开(公告)号：US20070161112A1
公开(公告)日：2007-07-12
申请号：US11622900
申请日：2007-01-12
申请人： Kyle Gee , Jolene Bradford , Vladimir Martin
发明人： Kyle Gee , Jolene Bradford , Vladimir Martin
IPC分类号： G01N33/20 , C07D311/88 , C07D263/08 , C07D209/04 , C07D413/04
CPC分类号： C07D413/04 , C07D209/14 , C07D335/12 , C09B11/28
摘要： The present invention provides a metal chelator and methods that facilitate binding, detecting, monitoring and quantitating of heavy metal ions in a sample. This metal chelating moiety is a —N,N,O-triacetic acid analog of BAPTA and has the following formula
摘要翻译：本发明提供金属螯合剂和促进样品中重金属离子结合，检测，监测和定量的方法。该金属螯合部分是BAPTA的-N，N，O-三乙酸类似物，具有下式

3. 发明申请

US20100330584A1 LONG WAVELENGTH FLUOROGENIC INTRACELLULAR ION INDICATORS THAT ARE WELL RETAINED IN THE CYTOSOL 有权
标题翻译：长期存在于细胞周期中的长波长荧光细胞离子指示器
公开(公告)号：US20100330584A1
公开(公告)日：2010-12-30
申请号：US12602973
申请日：2008-06-05
申请人： Kyle Gee , Vladimir Martin
发明人： Kyle Gee , Vladimir Martin
IPC分类号： G01N33/53 , C12N5/00 , C07D413/10 , C07D311/82
CPC分类号： G01N33/84 , A61K31/353 , C07D311/90 , C07D413/04 , C07D413/10 , C07D491/04 , C07D491/147 , C09B11/24 , G01N33/582 , G01N33/6872 , G01N2223/406 , G01N2458/00
摘要： Cell permeable metal ion indicator compounds and methods of their use and synthesis are described. The compound comprises a metal chelating moiety (Mc), a reporter molecule and two or more lipophilic groups (GL) covalently bonded through a linker to the reporter molecule, wherein the lipophilic groups, when present in a live cell, are cleaved resulting in two or more negatively charged groups.
摘要翻译：描述了细胞可渗透金属离子指示剂化合物及其使用和合成方法。化合物包括金属螯合部分（Mc），报告分子和通过接头共价键合到报告分子上的两个或多个亲油基团（GL），其中亲和基团当存在于活细胞中时被切割，导致两个或更多带负电的组。

4. 发明授权

US07102005B2 Compositions and methods for detection and isolation of phosphorylated molecules 有权
标题翻译：用于磷酸化分子检测和分离的组合物和方法
公开(公告)号：US07102005B2
公开(公告)日：2006-09-05
申请号：US10428192
申请日：2003-05-02
申请人： Brian Agnew , Joseph Beechem , Kyle Gee , Richard Haugland , Jixiang Liu , Vladimir Martin , Wayne Patton , Thomas Steinberg
发明人： Brian Agnew , Joseph Beechem , Kyle Gee , Richard Haugland , Jixiang Liu , Vladimir Martin , Wayne Patton , Thomas Steinberg
IPC分类号： C07D239/88 , C07D277/62 , C07D311/82 , C07D417/02 , C07D495/04
CPC分类号： G01N33/50 , G01N33/5008 , G01N33/502 , G01N33/5091 , G01N2333/4709
摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
摘要翻译：本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在磷酸化结合化合物和导致三元复合物的磷酸化靶分子。本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。

5. 发明申请

US20050014197A1 Compositions and methods for detection and isolation of phosphorylated molecules 有权
标题翻译：用于磷酸化分子检测和分离的组合物和方法
公开(公告)号：US20050014197A1
公开(公告)日：2005-01-20
申请号：US10821522
申请日：2004-04-09
申请人： Brian Agnew , Kyle Gee , Vladimir Martin
发明人： Brian Agnew , Kyle Gee , Vladimir Martin
IPC分类号： C12Q1/48 , G01N33/53
CPC分类号： G01N33/5008 , G01N33/502 , G01N33/5091 , G01N2333/4709
摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
摘要翻译：本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在磷酸化结合化合物和导致三元复合物的磷酸化靶分子。本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。

6. 发明授权

US09341631B2 Long wavelength fluorogenic intracellular ion indicators that are well retained in the cytosol 有权
标题翻译：长波长的荧光细胞内离子指示剂，很好地保留在细胞溶质中
公开(公告)号：US09341631B2
公开(公告)日：2016-05-17
申请号：US14302175
申请日：2014-06-11
申请人： Kyle Gee , Vladimir Martin
发明人： Kyle Gee , Vladimir Martin
IPC分类号： A61K31/353 , C07D311/90 , C07D413/04 , C07D491/04 , G01N33/84 , C07D413/10 , C07D491/147 , C09B11/24 , G01N33/58 , G01N33/68
CPC分类号： G01N33/84 , A61K31/353 , C07D311/90 , C07D413/04 , C07D413/10 , C07D491/04 , C07D491/147 , C09B11/24 , G01N33/582 , G01N33/6872 , G01N2223/406 , G01N2458/00
摘要： Cell permeable metal ion indicator compounds and methods of their use and synthesis are described. The compound comprises a metal chelating moiety (Mc), a reporter molecule and two or more lipophilic groups (GL) covalently bonded through a linker to the reporter molecule, wherein the lipophilic groups, when present in a live cell, are cleaved resulting in two or more negatively charged groups. An exemplary compound for use as a cell permeable metal ion indicator is represented by the structure:
摘要翻译：描述了细胞可渗透金属离子指示剂化合物及其使用和合成方法。化合物包括金属螯合部分（Mc），报告分子和通过接头共价键合到报告分子上的两个或多个亲油基团（GL），其中亲和基团当存在于活细胞中时被切割，导致两个或更多带负电的组。用作细胞可渗透金属离子指示剂的示例性化合物由以下结构表示：

7. 发明授权

US08785208B2 Fluorescent metal ion indicators with large stokes shifts 有权
标题翻译：荧光金属离子指示器具有大的斯托克斯位移
公开(公告)号：US08785208B2
公开(公告)日：2014-07-22
申请号：US13194727
申请日：2011-07-29
申请人： Kyle Gee , Vladimir Martin
发明人： Kyle Gee , Vladimir Martin
IPC分类号： G01N21/76 , C07D209/14 , C07D311/82 , C07D311/90 , C07D413/04
CPC分类号： G01N33/84 , C07C229/18 , C07C311/44 , C07D209/14 , C07D221/14 , C07D265/38 , C07D311/82 , C07D311/90 , C07D413/04 , C07K14/195 , C07K14/43595 , C09B5/60 , C09B5/62 , C09B11/06 , C09B11/08 , C09B19/00 , C09B19/005 , C09B57/08 , C09K11/06 , C09K2211/1014 , G01N33/20 , G01N2458/00
摘要： The present invention provides fluorogenic compounds for the detection of target metal ions wherein the compounds exhibit a Stokes shift greater than 50 nm and the detectable signal is modulated by photoinduced electron transfer (PET). The present compounds consist of three functional elements, the ion sensing moiety (chelating moiety), the reporter moiety (fluorophore or fluorescent protein) and spacer or linker between the sensing and reporter moieties of the present compound that allows for PET upon binding of a metal ion and excitation by an appropriate wavelength.
摘要翻译：本发明提供用于检测靶金属离子的荧光化合物，其中化合物表现出大于50nm的斯托克斯位移，并且可检测信号通过光诱导电子转移（PET）调节。本发明化合物由三种功能元件组成，即离子感测部分（螯合部分），报道部分（荧光团或荧光蛋白）以及本发明化合物的感测和报道部分之间的间隔物或接头，其允许在结合金属时的PET 通过适当的波长离子和激发。

8. 发明授权

US08540521B2 Benzoxazole-based fluorescent metal ion indicators 有权
公开(公告)号：US08540521B2
公开(公告)日：2013-09-24
申请号：US13142584
申请日：2009-12-29
申请人： Kyle Gee , Vladimir Martin
发明人： Kyle Gee , Vladimir Martin
IPC分类号： C12Q1/02 , C07D455/04 , C07D417/04 , C07D413/10 , C07D263/56 , C07D491/147 , G01N21/75
CPC分类号： G01N21/75 , C07D263/56 , C07D263/57 , C07D401/04 , C07D413/04 , C07D417/04 , C07D471/06 , C07D491/16 , C09B57/00 , C09B57/02 , C09K11/06 , C09K2211/1007 , C09K2211/1014 , C09K2211/1029 , C09K2211/1033 , C09K2211/1037 , C09K2211/1048 , C09K2211/1088 , G01N33/582 , G01N33/84
摘要： Disclosed are benzoxazole-based compounds, kits, and methods of producing and using the described compounds in fluorescence-based detection of analytes (e.g., metal ions). Also disclosed are uses of benzoxazole-based compounds as ratiometric metal ion indicators.

9. 发明申请

US20110306082A1 "BENZOXAZOLE-BASED FLUORESCENT METAL ION INDICATORS" 有权
标题翻译： “基于苯并噻唑的荧光金属离子指示剂”
公开(公告)号：US20110306082A1
公开(公告)日：2011-12-15
申请号：US13142584
申请日：2009-12-29
申请人： Kyle Gee , Vladimir Martin
发明人： Kyle Gee , Vladimir Martin
IPC分类号： C12Q1/02 , C07D455/04 , C07D417/04 , G01N21/75 , C07D413/10 , C07D263/56 , C07D491/147 , C07D263/57 , C07D413/04
CPC分类号： G01N21/75 , C07D263/56 , C07D263/57 , C07D401/04 , C07D413/04 , C07D417/04 , C07D471/06 , C07D491/16 , C09B57/00 , C09B57/02 , C09K11/06 , C09K2211/1007 , C09K2211/1014 , C09K2211/1029 , C09K2211/1033 , C09K2211/1037 , C09K2211/1048 , C09K2211/1088 , G01N33/582 , G01N33/84
摘要： Disclosed are benzoxazole-based compounds, kits, and methods of producing and using the described compounds in fluorescence-based detection of analytes (e.g., metal ions). Also disclosed are uses of benzoxazole-based compounds as ratiometric metal ion indicators.
摘要翻译：公开了基于苯并恶唑的化合物，试剂盒以及在分析物（例如金属离子）的基于荧光的检测中制备和使用所述化合物的方法。还公开了苯并恶唑类化合物作为比例金属离子指示剂的用途。

10. 发明申请

US20080274907A1 FLUOROGENIC PH SENSITIVE DYES AND THEIR METHOD OF USE 审中-公开
标题翻译：荧光敏感性染料及其使用方法
公开(公告)号：US20080274907A1
公开(公告)日：2008-11-06
申请号：US11927588
申请日：2007-10-29
申请人： Daniel Beacham , Jeffrey Dzubay , Kyle Gee , Vladimir Martin , Aleksey Rukavishnikov
发明人： Daniel Beacham , Jeffrey Dzubay , Kyle Gee , Vladimir Martin , Aleksey Rukavishnikov
IPC分类号： C07D455/04 , C07D311/82 , C07D209/12 , C40B30/00 , G01N33/68 , G01N21/00 , C12Q1/02 , C07F5/02 , C07D413/10
CPC分类号： G01N33/84 , C07D209/14 , C07D311/82 , C07D311/90 , C07D405/12 , C07D413/04 , C07D491/22 , C07F5/02 , C07K1/13 , C09B7/00 , C09B11/12 , C09B11/24 , C09B11/28 , C09B57/00 , C09B69/00 , C09K11/06 , G01N31/22 , G01N33/5005 , G01N33/5058 , G01N33/5091 , G01N33/582 , G01N33/80 , G01N2333/245 , Y10T436/143333
摘要： A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I: wherein the variables are described throughout the application.
摘要翻译：描述了一类新的pH敏感荧光染料和与其相关的测定。染料和测定特别适用于生物学应用，包括吞噬和监测细胞内过程。本发明的pH敏感荧光染料包括式I化合物：其中在整个申请中描述了变量。

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