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    • 5. 发明申请
    • Compositions and methods for detection and isolation of phosphorylated molecules
    • 用于磷酸化分子检测和分离的组合物和方法
    • US20050014197A1
    • 2005-01-20
    • US10821522
    • 2004-04-09
    • Brian AgnewKyle GeeVladimir Martin
    • Brian AgnewKyle GeeVladimir Martin
    • C12Q1/48G01N33/53
    • G01N33/5008G01N33/502G01N33/5091G01N2333/4709
    • The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
    • 本发明涉及用于结合,检测和分离磷酸化靶分子的磷酸盐结合化合物,包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。 提供的结合溶液包括磷酸盐结合化合物,酸和金属离子,其中金属离子同时与靶分子上暴露的磷酸基团相互作用,并且磷酸盐结合化合物的金属螯合部分形成在 磷酸化结合化合物和导致三元复合物的磷酸化靶分子。 本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子,从复杂混合物中分离磷酸化靶分子,并辅助蛋白质组分析,其中可以鉴定激酶和磷酸酶底物和酶。