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1. 发明授权

US07102005B2 Compositions and methods for detection and isolation of phosphorylated molecules 有权
标题翻译：用于磷酸化分子检测和分离的组合物和方法
公开(公告)号：US07102005B2
公开(公告)日：2006-09-05
申请号：US10428192
申请日：2003-05-02
申请人： Brian Agnew , Joseph Beechem , Kyle Gee , Richard Haugland , Jixiang Liu , Vladimir Martin , Wayne Patton , Thomas Steinberg
发明人： Brian Agnew , Joseph Beechem , Kyle Gee , Richard Haugland , Jixiang Liu , Vladimir Martin , Wayne Patton , Thomas Steinberg
IPC分类号： C07D239/88 , C07D277/62 , C07D311/82 , C07D417/02 , C07D495/04
CPC分类号： G01N33/50 , G01N33/5008 , G01N33/502 , G01N33/5091 , G01N2333/4709
摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
摘要翻译：本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在磷酸化结合化合物和导致三元复合物的磷酸化靶分子。本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。

2. 发明申请

US20060063269A1 Fluorescent isotope tags and their method of use 审中-公开
标题翻译：荧光同位素标签及其使用方法
公开(公告)号：US20060063269A1
公开(公告)日：2006-03-23
申请号：US11157467
申请日：2005-06-20
申请人： Brian Agnew , Kyle Gee
发明人： Brian Agnew , Kyle Gee
IPC分类号： G01N33/48
CPC分类号： G01N33/582 , C09B11/24 , G01N33/533 , G01N33/534 , G01N33/583 , G01N33/60 , G01N33/6848
摘要： The present invention provides novel reactive fluorescent compounds that incorporate stable isotopic (deuterium, 13-carbon, 15-nitrogen, 18-oxygen) substitutions. The invention includes the use of these compounds, in combination with non-isotopically substituted analogs, for the purification, identification and relative quantification of proteins, peptides, saccharides, metabolites, and other biologically important compounds by combining liquid chromatography (LC) and mass spectrometry (MS). Fluorescent labeling of target compounds in this manner provides orders-of-magnitude sensitivity enhancement over traditional stable isotope labels, and also affords the possibility of simultaneous multiplexed analysis due to the multiwavelength nature of different fluorophores.
摘要翻译：本发明提供了引入稳定同位素（氘，13-碳，15-氮，18-氧）取代的新型反应性荧光化合物。本发明包括将这些化合物与非同位素取代的类似物组合用于通过液相色谱（LC）和质谱法的组合来纯化，鉴定和相对定量蛋白质，肽，糖，代谢物和其它生物学上重要的化合物（女士）。目标化合物的荧光标记以这种方式提供超过传统稳定同位素标记的数量级的灵敏度增强，并且由于不同荧光团的多波长性质，也提供同时多重分析的可能性。

3. 发明申请

US20050014197A1 Compositions and methods for detection and isolation of phosphorylated molecules 有权
标题翻译：用于磷酸化分子检测和分离的组合物和方法
公开(公告)号：US20050014197A1
公开(公告)日：2005-01-20
申请号：US10821522
申请日：2004-04-09
申请人： Brian Agnew , Kyle Gee , Vladimir Martin
发明人： Brian Agnew , Kyle Gee , Vladimir Martin
IPC分类号： C12Q1/48 , G01N33/53
CPC分类号： G01N33/5008 , G01N33/502 , G01N33/5091 , G01N2333/4709
摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
摘要翻译：本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在磷酸化结合化合物和导致三元复合物的磷酸化靶分子。本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。

4. 发明申请

US20100009381A1 Compositions and methods for detection and isolation of phosphorylated molecules 审中-公开
标题翻译：用于磷酸化分子检测和分离的组合物和方法
公开(公告)号：US20100009381A1
公开(公告)日：2010-01-14
申请号：US12500854
申请日：2009-07-10
申请人： Brian Agnew , Joseph Beechem , Kyle Gee , Richard Haugland , Thomas Steinberg , Wayne Patton
发明人： Brian Agnew , Joseph Beechem , Kyle Gee , Richard Haugland , Thomas Steinberg , Wayne Patton
IPC分类号： G01N33/53
CPC分类号： G01N33/5008 , C12Q1/42 , C12Q1/485 , G01N33/502 , G01N33/5091 , G01N2333/4709 , Y10T436/142222 , Y10T436/145555 , Y10T436/147777 , Y10T436/201666
摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
摘要翻译：本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在磷酸化结合化合物和导致三元复合物的磷酸化靶分子。本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。

5. 发明申请

US20110021372A1 Compositions and methods for detection and isolation of phosphorylated molecules 审中-公开
标题翻译：用于磷酸化分子检测和分离的组合物和方法
公开(公告)号：US20110021372A1
公开(公告)日：2011-01-27
申请号：US12821003
申请日：2010-06-22
申请人： Brian AGNEW , Joseph Beechem , Kyle Gee , Richard Haugland , Thomas Steinberg , Wayne Patton
发明人： Brian AGNEW , Joseph Beechem , Kyle Gee , Richard Haugland , Thomas Steinberg , Wayne Patton
IPC分类号： C40B30/04 , C12Q1/68 , G01N33/68 , G01N33/566
CPC分类号： G01N33/5008 , C12Q1/42 , C12Q1/485 , G01N33/502 , G01N33/5091 , G01N2333/4709 , Y10T436/142222 , Y10T436/145555 , Y10T436/147777 , Y10T436/201666
摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
摘要翻译：本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在磷酸化结合化合物和导致三元复合物的磷酸化靶分子。本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。

6. 发明申请

US20080044804A1 COMPOSITIONS AND METHODS FOR DETECTION AND ISOLATION OF PHOSPHORYLATED MOLECULES 审中-公开
标题翻译：用于检测和分离磷酸化分子的组合物和方法
公开(公告)号：US20080044804A1
公开(公告)日：2008-02-21
申请号：US11697103
申请日：2007-04-05
申请人： Brian AGNEW , Joseph Beechem , Kyle Gee , Richard Haugland , Thomas Steinberg , Wayne Patton
发明人： Brian AGNEW , Joseph Beechem , Kyle Gee , Richard Haugland , Thomas Steinberg , Wayne Patton
IPC分类号： G01N33/00 , C12Q1/00 , G01N33/20
CPC分类号： G01N33/5008 , C12Q1/42 , C12Q1/485 , G01N33/502 , G01N33/5091 , G01N2333/4709 , Y10T436/142222 , Y10T436/145555 , Y10T436/147777 , Y10T436/201666
摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
摘要翻译：本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在磷酸化结合化合物和导致三元复合物的磷酸化靶分子。本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。

7. 发明申请

US20070099222A1 Methods and compositions for labeling nucleic acids 有权
标题翻译：用于标记核酸的方法和组合物
公开(公告)号：US20070099222A1
公开(公告)日：2007-05-03
申请号：US11588697
申请日：2006-10-27
申请人： Kyle Gee , Brian Agnew
发明人： Kyle Gee , Brian Agnew
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6806 , C12Q1/68 , C12Q1/6816 , C12Q1/6841 , G01N33/5011 , G01N33/582 , C12Q2525/101 , C12Q2563/107
摘要： The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation. The methods of the invention can be used in a wide variety of applications including clinical diagnosis of diseases and disorders in which cellular proliferation is involved, toxicity assays, and as a tool for the study of chromosomes' ultrastructures.
摘要翻译：本发明涉及体外和体内标记核酸聚合物的方法。提供了包括掺入核酸聚合物的核苷酸类似物与附着于标记物上的试剂之间的[3 + 2]环加成的某些方法。提供了包括掺入核酸聚合物的核苷酸类似物与包含连接到标签上的取代的三芳基膦的试剂之间的施陶丁格连接的其它方法。这种方法不需要固定和变性，因此可以应用于活细胞和生物体中核酸聚合物的标记。还提供了测量细胞增殖的方法。在这些方法中，测量结合到DNA中的标记量作为细胞增殖的指示。本发明的方法可用于各种应用，包括涉及细胞增殖的疾病和病症的临床诊断，毒性测定以及用于研究染色体超微结构的工具。

8. 发明授权

US08785212B2 Oligosaccharide modification and labeling of proteins 有权
标题翻译：寡糖修饰和蛋白质标记
公开(公告)号：US08785212B2
公开(公告)日：2014-07-22
申请号：US13007506
申请日：2011-01-14
申请人： Brian Agnew , Kyle Gee , Schuyler Corry
发明人： Brian Agnew , Kyle Gee , Schuyler Corry
IPC分类号： G01N33/532 , G01N33/534 , G01N33/533 , G01N33/50 , A61K47/48
CPC分类号： C12P21/005 , A61K47/549 , A61K47/61 , C07K1/13 , C07K14/47 , C07K16/00 , C07K2317/14 , C07K2317/24 , C07K2317/41 , C12Y302/01096 , G01N33/5005 , G01N33/5008 , G01N33/531 , G01N33/532 , G01N33/533 , G01N33/534 , G01N33/581 , G01N33/582 , G01N33/583 , Y10T436/17
摘要： The present invention generally relates to methods of functionalizing proteins, particularly antibodies, at oligosaccharide linkages, methods of humanizing antibodies by modifying glycosylation, as well as to novel antibodies linked to modified oligosaccharides. The invention further relates to kits that may be used to produce the antibodies of the invention.
摘要翻译：本发明通常涉及在寡糖键上官能化蛋白质，特别是抗体的方法，通过修饰糖基化来人源化抗体的方法，以及与修饰的寡糖连接的新型抗体。本发明还涉及可用于产生本发明抗体的试剂盒。

9. 发明申请

US20070190597A1 OLIGOSACCHARIDE MODIFICATION AND LABELING OF PROTEINS 有权
标题翻译：蛋白质的寡糖修饰和标记
公开(公告)号：US20070190597A1
公开(公告)日：2007-08-16
申请号：US11674136
申请日：2007-02-12
申请人： Brian AGNEW , Schuyler Corry , Kyle Gee
发明人： Brian AGNEW , Schuyler Corry , Kyle Gee
IPC分类号： C12P21/06 , C07K16/46
CPC分类号： C12P21/005 , A61K47/549 , A61K47/61 , C07K1/13 , C07K14/47 , C07K16/00 , C07K2317/14 , C07K2317/24 , C07K2317/41 , C12Y302/01096 , G01N33/5005 , G01N33/5008 , G01N33/531 , G01N33/532 , G01N33/533 , G01N33/534 , G01N33/581 , G01N33/582 , G01N33/583 , Y10T436/17
摘要： The present invention generally relates to methods of functionalizing proteins, particularly antibodies, at oligosaccharide linkages, methods of humanizing antibodies by modifying glycosylation, as well as to novel antibodies linked to modified oligosaccharides. The invention further relates to kits that may be used to produce the antibodies of the invention.
摘要翻译：本发明通常涉及在寡糖键上官能化蛋白质，特别是抗体的方法，通过修饰糖基化来人源化抗体的方法，以及与修饰的寡糖连接的新型抗体。本发明还涉及可用于产生本发明抗体的试剂盒。

10. 发明授权

US09334523B2 Substrates and methods for staining live stem cells 有权
标题翻译：底物和染色活体干细胞的方法
公开(公告)号：US09334523B2
公开(公告)日：2016-05-10
申请号：US13985559
申请日：2012-02-14
申请人： Uma Lakshmipathy , Upinder Singh , Scott Grecian , Rene Quintanilla , Kyle Gee , Mahendra Rao
发明人： Uma Lakshmipathy , Upinder Singh , Scott Grecian , Rene Quintanilla , Kyle Gee , Mahendra Rao
IPC分类号： C12Q1/42 , G01N33/50 , G01N33/58
CPC分类号： G01N33/5005 , C09B11/24 , C09B19/00 , C09B21/00 , C09B57/00 , C09B57/02 , C12Q1/42 , C12Y301/03001 , G01N1/30 , G01N33/5073 , G01N33/581 , G01N33/582 , G01N33/586 , G01N2001/302 , G01N2333/916
摘要： The invention relates to novel substrates and methods for staining live stem cells. The stain may be used to identify induced pluripotent stem cell colonies during the process of somatic cell reprogramming.
摘要翻译：本发明涉及用于染色活的干细胞的新的底物和方法。染色可用于在体细胞重编程过程中鉴定诱导的多能干细胞集落。

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