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31. 发明申请

US20080014608A1 Modified Promoter 失效
标题翻译：修饰的启动子
公开(公告)号：US20080014608A1
公开(公告)日：2008-01-17
申请号：US10589960
申请日：2005-03-04
申请人： Keiji Endo , Katsuya Ozaki
发明人： Keiji Endo , Katsuya Ozaki
IPC分类号： C12P21/04 , C07H21/04 , C12N1/20 , C12N15/00 , C12N15/11
CPC分类号： C12N15/75 , C07K14/32 , C12N9/2408 , C12N9/2411 , C12N9/2417 , C12N9/2437
摘要： The present invention provides a modified promoter DNA capable of enhancing transcription of genes encoding proteins or polypeptides, and a method for producing proteins or polypeptides efficiently by use of the modified promoter DNA.A promoter DNA recognized by SigA and SigE, which is produced by modifying a nucleotide sequence including a promoter recognized by SigA and bases in the vicinity thereof; an expression vector harboring the promoter DNA; a recombinant microorganism containing the expression vector; and a method for producing proteins or polypeptides characterized by culturing the recombinant microorganism.
摘要翻译：本发明提供能够增强编码蛋白质或多肽的基因的转录的修饰的启动子DNA，以及通过使用经修饰的启动子DNA有效产生蛋白质或多肽的方法。由SigA和SigE鉴定的启动子DNA，其通过修饰包括SigA识别的启动子和其附近的碱基的核苷酸序列而产生; 携带启动子DNA的表达载体; 含有表达载体的重组微生物; 以及产生以培养重组微生物为特征的蛋白质或多肽的方法。

32. 发明申请

US20050181493A1 Mutant alpha-amylases 审中-公开
标题翻译：突变型α-淀粉酶
公开(公告)号：US20050181493A1
公开(公告)日：2005-08-18
申请号：US10988529
申请日：2004-11-16
申请人： Keiji Endo , Kazuaki Igarashi , Yasuhiro Hayashi , Hiroshi Hagihara , Katsuya Ozaki
发明人： Keiji Endo , Kazuaki Igarashi , Yasuhiro Hayashi , Hiroshi Hagihara , Katsuya Ozaki
IPC分类号： C11D3/386 , C12N1/21 , C12N9/28 , C12N15/56 , C11D7/42
CPC分类号： C12N9/2417 , C11D3/386
摘要： The invention relates to a mutant α-amylase obtained by making replacement or deletion of at least one of amino acid residues such as the 167th Gln, 169th Tyr and 178th Ala in the amino acid sequence set forth in SEQ ID NO:1 in an α-amylase having said amino acid sequence, or an α-amylase having a homology of at least 70% to said amino acid sequence, a gene encoding the mutant α-amylase, a vector, transformed cells, a process for producing a mutant α-amylase, comprising culturing the transformed cells, and a detergent composition comprising the mutant α-amylase. The mutant α-amylase of the invention has excellent properties of high resistance to chelating agents, high specific activity in an alkaline region and excellent stability to heat, and is hence useful for detergents for automatic dish washer, laundry detergents and the like.
摘要翻译：本发明涉及通过使SEQ ID NO：1所示的氨基酸序列中的至少一个氨基酸残基例如第167位Gln，第169位和第178位氨基酸置换成α位点而获得的突变型α-淀粉酶具有所述氨基酸序列的淀粉酶或与所述氨基酸序列具有至少70％同源性的α-淀粉酶，编码突变型α-淀粉酶的基因，载体，转化细胞，用于产生突变型α-淀粉酶的方法，包括培养转化细胞的淀粉酶和包含突变型α-淀粉酶的洗涤剂组合物。本发明的突变型α-淀粉酶具有优异的抗螯合剂抗性，在碱性区域中具有高比活性和优异的耐热稳定性，因此可用于自动洗碗机，衣物洗涤剂等的洗涤剂。

33. 发明授权

US4734824A Electrical insulating oil and electrical appliances impregnated with the same 失效
标题翻译：电绝缘油和电器浸渍相同
公开(公告)号：US4734824A
公开(公告)日：1988-03-29
申请号：US877272
申请日：1986-06-23
申请人： Atsushi Sato , Keiji Endo , Shigenobu Kawakami , Hitoshi Yanagishita , Shozo Hayashi
发明人： Atsushi Sato , Keiji Endo , Shigenobu Kawakami , Hitoshi Yanagishita , Shozo Hayashi
IPC分类号： C07C15/27 , C07C1/00 , C07C15/16 , C07C15/52 , C07C15/60 , C07C67/00 , H01B3/22 , H01G4/22
CPC分类号： H01B3/22 , H01G4/221
摘要： An electrical insulating oil which is suitable for use in impregnating electrical appliances such as capacitors, cables and transformers, especially those in which plastic materials are used partially or totally as dielectrics or insulating materials, and electrical appliances that are impregnated with the above electrical insulating oil. The electrical insulating oil of the invention contains at least one monoolefin and/or diolefin having three condensed or non-condensed aromatic rings.

34. 发明授权

US4686548A Pressure-sensitive recording material 失效
标题翻译：压敏记录材料
公开(公告)号：US4686548A
公开(公告)日：1987-08-11
申请号：US745317
申请日：1985-06-14
申请人： Naoya Takahashi , Keiji Endo , Eiichi Matsuzaka , Satoshi Narui , Atsushi Sato
发明人： Naoya Takahashi , Keiji Endo , Eiichi Matsuzaka , Satoshi Narui , Atsushi Sato
IPC分类号： B41M5/165 , B41M5/16 , B41M5/22
CPC分类号： B41M5/1655
摘要： A pressure-sensitive recording material of the type which is prepared by using an electron donating dye-precursor that produces a color when the dye-precursor is brought into contact with an electron accepting color developer. The pressure-sensitive recording material is characterized in that the solvent for said dye-precursor comprises an alkylated fraction which is prepared by alkylating a by-product fraction with at least one alkyl group having 1 to 5 carbon atoms, the by-product fraction containing the components within the boiling range of 255.degree. to 300.degree. C. on the atmospheric pressure basis and being obtained in the process for producing ethylbenzene or ethyltoluene from ethylene and benzene or toluene. The pressure-sensitive recording material of the invention has scarcely offensive odor and can develop a dense color with a higher color developing rate.
摘要翻译：一种压敏记录材料，其是通过使用当染料前体与电子接受显色剂接触时产生颜色的给电子染料前体制备的。压敏记录材料的特征在于，用于所述染料前体的溶剂包括烷基化部分，其通过用至少一个具有1至5个碳原子的烷基烷基化副产物部分制备，副产物部分含有该成分在大气压的基础上在255〜300℃的沸点范围内，并且在从乙烯和苯或甲苯生产乙苯或乙基甲苯的方法中得到。本发明的压敏记录材料几乎没有异味，并且可以产生具有更高显色速率的致密颜色。

35. 发明授权

US4654074A 2-chloro-N-(4,6-dimethoxy-5-pyrimidinyl)-N-[1,(1-py razolyl)ethyl]acetamide, herbicidal composition comprising said compound, and method of controlling weeds using said compound 失效
标题翻译： 2-氯-N-（4,6-二甲氧基-5-嘧啶基）-N- [1,1-（吡唑基）乙基]乙酰胺，包含所述化合物的除草组合物和使用所述化合物控制杂草的方法
公开(公告)号：US4654074A
公开(公告)日：1987-03-31
申请号：US829995
申请日：1986-02-18
申请人： Atsushi Goh , Mami Nakamura , Keiji Endo , Mitsuru Hikido
发明人： Atsushi Goh , Mami Nakamura , Keiji Endo , Mitsuru Hikido
IPC分类号： C07D403/12 , A01N43/56 , C07D521/00 , C07D247/02
CPC分类号： C07D231/12 , A01N43/56 , C07D233/56 , C07D249/08
摘要： 2-Chloro-N-(4,6-dimethoxy-5-pyrimidinyl)-N-[1-(1-pyrazolyl)ethyl]acetamide of the following formula: ##STR1## a herbicidal composition comprising a herbicidally effective amount of the compound of formula (I); and a method of controlling weeds which comprises applying a herbicidally effective amount of the formula (I) to the locus where the weeds are growing or will grow.
摘要翻译：具有下式的2-氯-N-（4,6-二甲氧基-5-嘧啶基）-N- [1-（1-吡唑基）乙基]乙酰胺：（I）除草组合物，其包含除草有效式（I）化合物的量; 以及控制杂草的方法，其包括将除草有效量的式（I）施用于杂草生长或将要生长的场所。

36. 发明授权

US4618914A Electrical insulating oil and oil-filled electrical appliances 失效
标题翻译：电绝缘油和充油电器
公开(公告)号：US4618914A
公开(公告)日：1986-10-21
申请号：US706408
申请日：1985-02-28
申请人： Atsushi Sato , Keiji Endo , Shigenobu Kawakami , Hitoshi Yanagishita , Shozo Hayashi
发明人： Atsushi Sato , Keiji Endo , Shigenobu Kawakami , Hitoshi Yanagishita , Shozo Hayashi
IPC分类号： H01B3/22 , H01B3/20 , H01G4/22
CPC分类号： H01G4/221 , H01B3/20
摘要： An improved electrical insulating oil which is stable in refining treatment and good electrical properties are maintained under high temperature conditions, and oil-filled electrical appliances impregnated therewith. The electrical insulating oil comprises 1,1,-diarylalkenes which are represented by the following general formula (I): ##STR1## wherein R.sub.1 to R.sub.4 's are the same or different and each of them is a hydrogen atom or an alkyl group having 1 to 5 carbon atoms, and m and n are integers from 0 to 3, inclusive.
摘要翻译：在高温条件下保持在精炼处理中稳定的良好电性能的改进的电绝缘油，以及浸渍在其中的充油电器。电绝缘油包含由以下通式（I）表示的1,1,1-二烯基烯烃：其中R 1至R 4相同或不同并且各自为氢原子或烷基具有1至5个碳原子，m和n是0至3的整数，包括0和3。

37. 发明授权

US4549034A Refined electrical insulating oil and oil-filled electrical appliances 失效
标题翻译：精密电绝缘油和充油电器
公开(公告)号：US4549034A
公开(公告)日：1985-10-22
申请号：US588654
申请日：1984-03-12
申请人： Atsushi Sato , Keiji Endo , Shigenobu Kawakami , Hitoshi Yanagishita , Shozo Hayashi
发明人： Atsushi Sato , Keiji Endo , Shigenobu Kawakami , Hitoshi Yanagishita , Shozo Hayashi
IPC分类号： C10M105/06 , A61B18/00 , C10M177/00 , C10N40/16 , C10N70/00 , H01B3/22 , H01G4/22
CPC分类号： H01B3/22 , A61B18/00 , H01G4/221
摘要： A refined electrical insulating oil and oil-filled electrical appliances impregnated therewith. The electrical insulating oil is quite suitable for use in oil-filled electrical appliances in which insulating materials or dielectric materials made of plastics are employed. The electrical insulating oil is prepared by refining a mixture of unsaturated dimers of styrenes and alkylbiphenyls and/or alkylnaphthalenes, using a solid acidic substance.
摘要翻译：精炼的电绝缘油和浸油的电油器具。电绝缘油非常适用于使用由塑料制成的绝缘材料或介电材料的充油电器。电绝缘油通过使用固体酸性物质精制苯乙烯和烷基联苯和/或烷基萘的不饱和二聚体的混合物来制备。

38. 发明授权

US4378990A Herbicidal composition 失效
标题翻译：除草成分
公开(公告)号：US4378990A
公开(公告)日：1983-04-05
申请号：US212679
申请日：1980-12-03
申请人： Keiji Endo , Tomomi Toriyama , Kisaku Mori
发明人： Keiji Endo , Tomomi Toriyama , Kisaku Mori
IPC分类号： A01N43/828 , A01N47/36 , A01N43/02 , A01N31/00
CPC分类号： A01N47/36
摘要： Herbicidal composition containing 5-(N-phenylcarbamoylamino)-1,2,3-thiadiazole and either a diphenyl ether of the formula ##STR1## or an acetanilide of the formula ##STR2## in which X is chlorine, methyl or trifluoromethyl, Y is hydrogen, lower alkoxy, ethoxyethoxyethoxy or methoxycarbonyl, n is 1 to 3, R.sub.1 and R.sub.2 are lower alkyl and R.sub.3 is lower alkoxy-lower alkyl. The active components display synergistic effectiveness as broad-spectrum herbicides for perennial as well as annual weeds in paddy fields. Suitable solid and liquid carrier materials for the composition include emulsifiers.
摘要翻译：含有5-（N-苯基氨基甲酰基氨基）-1,2,3-噻二唑和式“IMAGE”的二苯醚或式“IMAGE”的乙酰苯胺的除草组合物，其中X为氯，甲基或三氟甲基，Y为氢，低级烷氧基，乙氧基乙氧基乙氧基或甲氧基羰基，n为1至3，R 1和R 2为低级烷基，R 3为低级烷氧基 - 低级烷基。活性成分显示出作为广泛用于稻田多年生和一年生杂草的广谱除草剂的协同效应。用于组合物的合适的固体和液体载体材料包括乳化剂。

39. 发明授权

US08940514B2 Thioesterase and a method of producing fatty acids or lipids using the thioesterase 有权
标题翻译：硫酯酶和使用硫酯酶生产脂肪酸或脂质的方法
公开(公告)号：US08940514B2
公开(公告)日：2015-01-27
申请号：US13696147
申请日：2011-04-13
申请人： Takuto Tojo , Keiji Endo
发明人： Takuto Tojo , Keiji Endo
IPC分类号： C12N9/18 , A01H1/06 , C12N15/00 , C12N1/20 , C12N15/82 , C12N9/16 , C12P7/64
CPC分类号： C12N15/8247 , C12N9/16 , C12P7/6418 , C12P7/6436 , C12Y301/02014
摘要： A thioesterase comprising an amino acid sequence set forth in SEQ ID NO: 1, a thioesterase gene encoding the thioesterase, a transformant comprising the gene, and a method of producing fatty acids or lipids using the transformant.
摘要翻译：包含SEQ ID NO：1所示氨基酸序列的硫酯酶，编码硫酯酶的硫酯酶基因，包含该基因的转化体，以及使用该转化体产生脂肪酸或脂质的方法。

40. 发明授权

US08293516B2 Recombinant microorganism 有权
标题翻译：重组微生物
公开(公告)号：US08293516B2
公开(公告)日：2012-10-23
申请号：US12528039
申请日：2007-12-27
申请人： Keiji Endo , Katsutoshi Ara
发明人： Keiji Endo , Katsutoshi Ara
IPC分类号： C12N1/20 , C12P21/06
CPC分类号： C12N15/75 , C07K14/32 , C12P21/02
摘要： A recombinant microorganism is provided that has increased productivity of a protein or polypeptide of interest. A method for producing a protein of interest using the microorganism is also provided. The microorganism is prepared by inserting, in its genome, a transcription initiation regulatory region or both a transcription initiation regulatory region and a ribosome-binding site, upstream of a Bacillus subtilis prsA gene and by deleting or inactivating one or more of the abrB gene, dltA gene, dltB gene, dltC gene, dltD gene, dltE gene, or a gene (genes) corresponding thereto.
摘要翻译：提供了具有增加的感兴趣的蛋白质或多肽的生产力的重组微生物。还提供了使用该微生物生产感兴趣的蛋白质的方法。通过在其基因组中插入转录起始调节区或在枯草芽孢杆菌prsA基因的上游插入转录起始调节区和核糖体结合位点，以及通过缺失或失活一个或多个abrB基因， dltA基因，dltB基因，dltC基因，dltD基因，dltE基因或与其对应的基因（基因）。

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