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    • 36. 发明申请
    • ENZYMATIC PRODUCTION OF AN ETHYLENICALLY UNSATURATED GLYCOSIDE
    • 乙酰甘油的乙酰化生产
    • US20120028308A1
    • 2012-02-02
    • US13192771
    • 2011-07-28
    • Harald KELLERKatja LoosWouter Kloosterman
    • Harald KELLERKatja LoosWouter Kloosterman
    • C12P19/44
    • C12P19/44C07H15/10C12P19/14C12Y302/01021
    • Ethylenically unsaturated glycosides of formula I wherein n, A, X, R3 and R4 have the meanings given in the description are produced by reacting an ethylenically unsaturated alcohol of formula II with a saccharide of formula III in the presence of a glycosidase, such as an amylase, cellulase, glucosidase or galactosidase, (i) at a molar ratio of ethylenically unsaturated alcohol of formula II to saccharide of formula III of from 2:1 to 30:1; (ii) in the presence of a solvent mixture of water and a water miscible organic solvent that is no primary or secondary alcohol, such as acetone, acetonitrile, t-pentanol, t-butanol, 1,4-dioxane and tetrahydrofuran, at a weight ratio of water to organic solvent of from 0.1:1 to 9:1; and (iii) at a weight ratio of solvent mixture to saccharide of from 3:1 to 30:1.
    • 其中n,A,X,R 3和R 4具有本说明书中给出的含义的式I的烯键式不饱和糖苷通过式Ⅱ的烯属不饱和醇与式III的糖在糖苷酶例如 淀粉酶,纤维素酶,葡糖苷酶或半乳糖苷酶,(i)以式II的烯键式不饱和醇与式III的糖的摩尔比为2:1至30:1; (ii)在水和水混溶性有机溶剂的混合物存在下,其不是伯或仲醇,例如丙酮,乙腈,叔戊醇,叔丁醇,1,4-二恶烷和四氢呋喃,在 水与有机溶剂的重量比为0.1:1至9:1; 和(iii)溶剂混合物与糖的重量比为3:1至30:1。